BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0059.p (256 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIL6.1 B3KIL6_9BACI NUDIX hydrolase 434 e-120 B1DD56.1 B1DD56_9BACL NUDIX hydrolase 303 7e-81 C0Z6C4.1 C0Z6C4_BREBN Putative uncharacterized protein 249 2e-64 B1HY80.1 B1HY80_LYSSC MutT/NUDIX family protein 196 1e-48 B1D579.1 B1D579_9BACL NUDIX hydrolase 195 3e-48 >B3KIL6.1 B3KIL6_9BACI NUDIX hydrolase Length = 261 Score = 434 bits (1115), Expect = e-120, Method: Compositional matrix adjust. Identities = 206/247 (83%), Positives = 217/247 (87%) Query: 10 NVKKYRTPDGVPADIVMFTLTKRERKTVTKTLPLRELKVMLVKRKKWPYAGMWALPGGFC 69 + KKYRTPDGVPADIVMFTLTKRERKT TKTLP+RELKVML++R+ WP AGMWALPGGFC Sbjct: 15 SAKKYRTPDGVPADIVMFTLTKRERKTFTKTLPIRELKVMLIRRRSWPCAGMWALPGGFC 74 Query: 70 QEDESIYDAATRELKEETGVDGGHLEYLGVYSKPGRDPRGWIISHAFFALVEEWMLEHRQ 129 +E ESIYDAA RELKEETGVDGGHLEYLGVYS PGRDPRGWIISHAF+ALVEEWMLEHRQ Sbjct: 75 RESESIYDAAKRELKEETGVDGGHLEYLGVYSAPGRDPRGWIISHAFYALVEEWMLEHRQ 134 Query: 130 ASDDAGEVGLFTXXXXXXXXXXXFDHHDIITDAYLRIQQQMLQTTIARQFLPHHFTLSEL 189 A+DDA EVGLFT FDH DII DAY +IQ QMLQTTIARQFLP HFTLSEL Sbjct: 135 AADDAEEVGLFTVAEALEELELAFDHRDIIMDAYRKIQLQMLQTTIARQFLPEHFTLSEL 194 Query: 190 YQVIQTVVPEFKEPNFIRKITSTRSRQGILKEVRDEAGNPVSSNQYSQRPAQLYMFTDHE 249 YQVIQTVVPEF EPNFIRKITSTRSRQGIL+E RDE G P+SSNQYSQRPAQLY FTDH Sbjct: 195 YQVIQTVVPEFNEPNFIRKITSTRSRQGILEEARDENGKPLSSNQYSQRPAQLYRFTDHA 254 Query: 250 PLLSIYT 256 PLLSIYT Sbjct: 255 PLLSIYT 261 >B1DD56.1 B1DD56_9BACL NUDIX hydrolase Length = 256 Score = 303 bits (777), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 149/256 (58%), Positives = 187/256 (73%), Gaps = 2/256 (0%) Query: 1 VSENEEQTYNVKKYRTPDGVPADIVMFTLTKRERKTVTKTLPLRELKVMLVKRKKWPYAG 60 ++ENE Y+ YRTPDG P DIV+FT+T +E++ K LP REL+V+L++RK WP+ G Sbjct: 3 LNENE-TNYSASNYRTPDGAPTDIVIFTITSQEKEKGKKALPNRELQVLLIRRKVWPFEG 61 Query: 61 MWALPGGFCQEDESIYDAATRELKEETGVDGGHLEYLGVYSKPGRDPRGWIISHAFFALV 120 WALPGGF +E E++ + A RELKEE GV+ +E+L VYSKPGRDPRGW+ISHAF ALV Sbjct: 62 QWALPGGFTRETETVEECAIRELKEEAGVERVRMEFLKVYSKPGRDPRGWMISHAFCALV 121 Query: 121 EEWMLEHRQASDDAGEVGLFTXXXXXXXXXXXFDHHDIITDAYLRIQQQMLQTTIARQFL 180 E L+ R+ASDDA +V LF FDHH II DAY RIQQQM+ IA++FL Sbjct: 122 NERYLKDRKASDDAEDVQLFP-LSEALEMELAFDHHGIIRDAYRRIQQQMVTEPIAKEFL 180 Query: 181 PHHFTLSELYQVIQTVVPEFKEPNFIRKITSTRSRQGILKEVRDEAGNPVSSNQYSQRPA 240 P FT+ ELYQ+IQTVVP F+E NFIRK+TST+SR+G+++EVR G+ SNQYSQR A Sbjct: 181 PEEFTIGELYQIIQTVVPAFEEKNFIRKVTSTQSRKGLIEEVRHADGSLKQSNQYSQRAA 240 Query: 241 QLYMFTDHEPLLSIYT 256 QLY FT P LSIY+ Sbjct: 241 QLYRFTGFVPELSIYS 256 >C0Z6C4.1 C0Z6C4_BREBN Putative uncharacterized protein Length = 262 Score = 249 bits (635), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 128/251 (50%), Positives = 165/251 (65%), Gaps = 5/251 (1%) Query: 9 YNVKKYRTPDGVPADIVMFTLTKRERKTVTKTLPLRELKVMLVKRKKWPYAGMWALPGGF 68 + KKYR+PDG+P DI +FTL R+T TK+LPL++L V+L+KRK + WALPGGF Sbjct: 13 FRTKKYRSPDGLPTDICLFTLLSIPRQTKTKSLPLKQLAVLLIKRKSNTFGEQWALPGGF 72 Query: 69 CQEDESIYDAATRELKEETGVDGG-HLEYLGVYSKPGRDPRGWIISHAFFALVEEWMLEH 127 E E++ + A RELKEET +D H+E L Y KPGRDPRGWI S A+ +LV E +L+H Sbjct: 73 SHESETLDECAYRELKEETNIDRDVHIEQLKTYYKPGRDPRGWIPSVAYVSLVHEDLLKH 132 Query: 128 RQASDDAGEVGLFTXXXXXXXXXXXFDHHDIITDAYLRIQQQMLQTTIARQFLPHHFTLS 187 QA+DDA + LF FDH +I+TDA R+++QMLQTTIA+ FLP FTLS Sbjct: 133 AQANDDASDAQLFP-IEEAFALNLAFDHREILTDALQRVREQMLQTTIAKAFLPDEFTLS 191 Query: 188 ELYQVIQTVVPEFKEP---NFIRKITSTRSRQGILKEVRDEAGNPVSSNQYSQRPAQLYM 244 EL QVI+TVVP F NF R++ +T RQG+L+ V G PV S ++S PA+LY Sbjct: 192 ELLQVIETVVPSFSAKDRGNFERRLLATTRRQGLLEPVLSADGQPVYSTEFSNSPAKLYR 251 Query: 245 FTDHEPLLSIY 255 FT P IY Sbjct: 252 FTGFTPTAMIY 262 >B1HY80.1 B1HY80_LYSSC MutT/NUDIX family protein Length = 266 Score = 196 bits (499), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 115/257 (44%), Positives = 154/257 (59%), Gaps = 15/257 (5%) Query: 9 YNVKKYRTPDGVPADIVMFTLTKRERKTVTKTLPLRELKVMLVKRKKWPY-------AGM 61 Y+ KY+TPDG ADI +FT+T KT K P LK+ML+KR G Sbjct: 14 YDSSKYKTPDGYTADIAIFTITSE--KTEEKAPPNMTLKIMLIKRATKDADGNANIEGGK 71 Query: 62 WALPGGF--CQEDESIYDAATRELKEETGVDGGHLEYLGVYSKPGRDPRGWIISHAFFAL 119 WALPGGF + ES Y AA RELKEET V+G H+++ GVY RDPRGW+IS+AF+A+ Sbjct: 72 WALPGGFVDAHQKESAYIAAKRELKEETNVEGLHVQHFGVYDDIDRDPRGWMISNAFYAI 131 Query: 120 VEEWMLEHRQASDDAGEVGLFTXXXXXXXXXXXFDHHDIITDAYLRIQQQMLQTTIARQF 179 V E +E RQA+DDA +V LF FDH +II DA I++ M+QTT+A+ F Sbjct: 132 VPEVYMEQRQANDDADDVELFD-IENVFTLQLAFDHRNIIADALEFIKRDMVQTTVAKNF 190 Query: 180 LPHHFTLSELYQVIQTVVPEFKEPNFIRKITSTRS-RQGILKEVRDEAGNPVSSNQYSQR 238 LP FTLSEL +V+ TV + + N + T++ + +++V D G P + + S R Sbjct: 191 LPQEFTLSELQRVLLTVEEDPRIAN--DSVFFTKAPKLPFIEKVVDAEGKPKKTKRNSFR 248 Query: 239 PAQLYMFTDHEPLLSIY 255 P+QLY F D E + SIY Sbjct: 249 PSQLYTFNDFEVVESIY 265 >B1D579.1 B1D579_9BACL NUDIX hydrolase Length = 263 Score = 195 bits (496), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 115/257 (44%), Positives = 151/257 (58%), Gaps = 16/257 (6%) Query: 8 TYNVKKYRTPDGVPADIVMFTLTKRERKTVTKTLPLRELKVMLVKRKKWPY-------AG 60 Y+ KKYRTPDG +DI +FT+ P R +K+ML++R + G Sbjct: 13 NYDPKKYRTPDGYTSDIAVFTIISE--PIAPNKPPKRTMKLMLIRRARHDREGSPNIEGG 70 Query: 61 MWALPGGFCQEDESIYDAATRELKEETGVDGGHLEYLGVYSKPGRDPRGWIISHAFFALV 120 WALPGGF +E Y+AA REL EETGV G H+ + GVY K GRD RGWIIS+A +A+V Sbjct: 71 KWALPGGFIDPEEVAYEAAKRELSEETGVQGIHIRHYGVYDKLGRDKRGWIISNAHYAIV 130 Query: 121 EEWMLEHRQASDDAGEVGLFTXXXXXXXXXXXFDHHDIITDAYLRIQQQMLQTTIARQFL 180 E LE R+A+DDA EV LFT FDH ++I DAY I++ ML +T+AR FL Sbjct: 131 PEDYLEKRKAADDAEEVALFT-IEEIKELELAFDHREVIADAYEMIRKDMLLSTVARSFL 189 Query: 181 PHHFTLSELYQVIQTVVPEFKEPNFIRKITSTRSRQGILK--EVRDEAGNPVSSNQYSQR 238 P FTLSEL V+ TV K + K ++ R+R L EV G ++++ S+R Sbjct: 190 PQEFTLSELQSVLLTVTDSPK----VAKYSTFRNRAERLPFIEVVRIDGEAKTTSRNSKR 245 Query: 239 PAQLYMFTDHEPLLSIY 255 P+ LY F + EPL SIY Sbjct: 246 PSILYRFKEGEPLESIY 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,518,777,842 Number of extensions: 59931040 Number of successful extensions: 137359 Number of sequences better than 1.0e-05: 559 Number of HSP's gapped: 137113 Number of HSP's successfully gapped: 559 Length of query: 256 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 123 Effective length of database: 1,807,701,208 Effective search space: 222347248584 Effective search space used: 222347248584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)