BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0057.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIL4.1 B3KIL4_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB E... 575 e-162 B1DLE1.1 B1DLE1_9BACL tRNA-i(6)A37 thiotransferase enzyme MiaB E... 551 e-155 C3J7B1.1 C3J7B1_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB E... 523 e-146 B7YFD5.1 B7YFD5_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB E... 523 e-146 Q5L0E8.1 MIAB_GEOKA (Dimethylallyl)adenosine tRNA methylthiotran... 521 e-146 >B3KIL4.1 B3KIL4_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB EC=1.3.1.74 Length = 519 Score = 575 bits (1483), Expect = e-162, Method: Compositional matrix adjust. Identities = 268/341 (78%), Positives = 296/341 (86%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREGMRAWVNIMYGCDKFC 60 +IFGTHN+HRLP L++EA FSKE+VVEVWSKEGDI+ENLPKKREG+R WVNIMYGCDKFC Sbjct: 173 LIFGTHNVHRLPHLIQEALFSKEMVVEVWSKEGDIVENLPKKREGLRGWVNIMYGCDKFC 232 Query: 61 TYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDLM 120 TYCIVP+TRGKERSRRPEDVIAE+RELARQG+KE+TLLGQNVNAYGKDF DI Y FGDLM Sbjct: 233 TYCIVPYTRGKERSRRPEDVIAEVRELARQGYKEITLLGQNVNAYGKDFEDIQYRFGDLM 292 Query: 121 DDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYSR 180 DD+ KIDIPR+RF TSHPRDFDD LIEVL KGGNL EHIHLPVQSGS+ VLKKMSRKY+R Sbjct: 293 DDIHKIDIPRVRFTTSHPRDFDDHLIEVLAKGGNLVEHIHLPVQSGSSQVLKKMSRKYTR 352 Query: 181 ETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPREG 240 ETYLEL KIK ++P+ TTDIIVGFPGET+EQFEETLSLVREVGYD AYTFIYSPREG Sbjct: 353 ETYLELAHKIKKAIPNVAFTTDIIVGFPGETDEQFEETLSLVREVGYDSAYTFIYSPREG 412 Query: 241 TPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSGR 300 TPAA MEDNVP EVKSERLQRLN I E SR SNDR+ SKNN ++LSGR Sbjct: 413 TPAAVMEDNVPMEVKSERLQRLNAAINEYSRKSNDRLLGSTVEVLVEGVSKNNDSMLSGR 472 Query: 301 SRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAV 341 +R NKLVHFEG EL+G+FV VRITDTKTWYIKGD + +A+ Sbjct: 473 TRTNKLVHFEGSTELVGSFVNVRITDTKTWYIKGDMIPDAI 513 >B1DLE1.1 B1DLE1_9BACL tRNA-i(6)A37 thiotransferase enzyme MiaB EC=1.3.1.74 Length = 525 Score = 551 bits (1420), Expect = e-155, Method: Compositional matrix adjust. Identities = 253/342 (73%), Positives = 291/342 (85%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREGMRAWVNIMYGCDKFC 60 MIFGTHNIHRLP L++ AYFSKE+VVEVWSKEGDIIEN+PKKR+GMRAWVNIMYGCDKFC Sbjct: 181 MIFGTHNIHRLPHLLQNAYFSKEMVVEVWSKEGDIIENMPKKRDGMRAWVNIMYGCDKFC 240 Query: 61 TYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDLM 120 TYCIVP+TRGKERSRRPEDVIAE+RELARQGFKE+TLLGQNVNAYGKDF DI Y FGDLM Sbjct: 241 TYCIVPYTRGKERSRRPEDVIAEVRELARQGFKEITLLGQNVNAYGKDFEDISYRFGDLM 300 Query: 121 DDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYSR 180 D+ KIDIPRIRF TSHPRDFDD LIEVL +GGNL EHIHLP+QSGST VLK+MSRKYSR Sbjct: 301 HDISKIDIPRIRFTTSHPRDFDDHLIEVLAQGGNLVEHIHLPIQSGSTEVLKRMSRKYSR 360 Query: 181 ETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPREG 240 E EL KIK ++P+ L+TDIIVGFPGET+EQFE+T++LVR++G+D AYTFIYSPREG Sbjct: 361 EQIFELAAKIKRAIPNVSLSTDIIVGFPGETDEQFEDTMTLVRQIGFDFAYTFIYSPREG 420 Query: 241 TPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSGR 300 TPAA MEDNVP E+K +RL+RLN+L+ E R N+RM SKNNPN+L+GR Sbjct: 421 TPAAGMEDNVPMELKKKRLKRLNELMAELGRRGNERMEGEIVEVLVEGESKNNPNILAGR 480 Query: 301 SRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAVS 342 +R NKLVH EGPKE IG F+ VR+T+ KTWYIK + +A + S Sbjct: 481 TRTNKLVHLEGPKEWIGQFIHVRVTEAKTWYIKAEPLANSAS 522 >C3J7B1.1 C3J7B1_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB EC=1.3.1.74 Length = 531 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 246/344 (71%), Positives = 284/344 (82%), Gaps = 1/344 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 +IFGTHNIHRLP ++ EAY SKE+VVEVWSKEGD++ENLPK R+G ++AWVNIMYGCDKF Sbjct: 179 LIFGTHNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGKIKAWVNIMYGCDKF 238 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 CTYCIVP+TRGKERSRRPED+I E+R+LA QG+KE+TLLGQNVNAYGKDFTDI Y+ GDL Sbjct: 239 CTYCIVPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFTDIQYSLGDL 298 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 MD++RKIDI RIRF TSHPRDFDDRLIEVL K GNL EHIHLPVQSGST +LK M RKY+ Sbjct: 299 MDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYT 358 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 RE YLELVRKIKA++PD LTTDIIVGFP ET+EQFEETLSL REV +D AYTFIYSPRE Sbjct: 359 REEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPRE 418 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 GTPAA+M+DNVP EVK ERL+RLNDL++E + + SK NP+VL+G Sbjct: 419 GTPAANMKDNVPMEVKKERLKRLNDLVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAG 478 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAVSI 343 +R NKLVHF GPK LIG V VRIT KTW + G+ V EA+ + Sbjct: 479 YTRKNKLVHFVGPKSLIGQLVNVRITQAKTWTLTGELVNEAIEV 522 >B7YFD5.1 B7YFD5_9BACI tRNA-i(6)A37 thiotransferase enzyme MiaB EC=1.3.1.74 Length = 531 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 246/344 (71%), Positives = 284/344 (82%), Gaps = 1/344 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 +IFGTHNIHRLP ++ EAY SKE+VVEVWSKEGD++ENLPK R+G ++AWVNIMYGCDKF Sbjct: 179 LIFGTHNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGKIKAWVNIMYGCDKF 238 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 CTYCIVP+TRGKERSRRPED+I E+R+LA QG+KE+TLLGQNVNAYGKDFTDI Y+ GDL Sbjct: 239 CTYCIVPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFTDIQYSLGDL 298 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 MD++RKIDI RIRF TSHPRDFDDRLIEVL K GNL EHIHLPVQSGST +LK M RKY+ Sbjct: 299 MDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYT 358 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 RE YLELVRKIKA++PD LTTDIIVGFP ET+EQFEETLSL REV +D AYTFIYSPRE Sbjct: 359 REEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPRE 418 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 GTPAA+M+DNVP EVK ERL+RLNDL++E + + SK NP+VL+G Sbjct: 419 GTPAANMKDNVPMEVKKERLKRLNDLVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAG 478 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAVSI 343 +R NKLVHF GPK LIG V VRIT KTW + G+ V EA+ + Sbjct: 479 YTRKNKLVHFVGPKSLIGQLVNVRITQAKTWTLTGELVNEAIEV 522 >Q5L0E8.1 MIAB_GEOKA (Dimethylallyl)adenosine tRNA methylthiotransferase miaB EC=2.-.-.- Length = 531 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 246/344 (71%), Positives = 283/344 (82%), Gaps = 1/344 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 +IFGTHNIHRLP ++ EAY SKE+VVEVWSKEGD++ENLPK R+G ++AWVNIMYGCDKF Sbjct: 179 LIFGTHNIHRLPYILHEAYMSKEMVVEVWSKEGDVVENLPKVRKGKIKAWVNIMYGCDKF 238 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 CTYCIVP+TRGKERSRRPED+I E+R+LA QG+KE+TLLGQNVNAYGKDFTDI Y GDL Sbjct: 239 CTYCIVPYTRGKERSRRPEDIIQEVRQLAAQGYKEITLLGQNVNAYGKDFTDIQYGLGDL 298 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 MD++RKIDI RIRF TSHPRDFDDRLIEVL K GNL EHIHLPVQSGST +LK M RKY+ Sbjct: 299 MDELRKIDIARIRFTTSHPRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYT 358 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 RE YLELVRKIKA++PD LTTDIIVGFP ET+EQFEETLSL REV +D AYTFIYSPRE Sbjct: 359 REEYLELVRKIKAAIPDVALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPRE 418 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 GTPAA+M+DNVP EVK ERL+RLNDL++E + + SK NP+VL+G Sbjct: 419 GTPAANMKDNVPMEVKKERLKRLNDLVQEIAAKKMKQYEGQVVEVLVEGESKTNPDVLAG 478 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAVSI 343 +R NKLVHF GPK LIG V VRIT KTW + G+ V EA+ + Sbjct: 479 YTRKNKLVHFVGPKSLIGQLVNVRITQAKTWTLTGELVNEAIEV 522 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,021,720,974 Number of extensions: 83137253 Number of successful extensions: 222478 Number of sequences better than 1.0e-05: 3052 Number of HSP's gapped: 217827 Number of HSP's successfully gapped: 3071 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)