BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0051.p (425 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A0AG51.1 A0AG51_LISW6 Complete genome EC=1.1.1.1 521 e-146 C4BX16.1 C4BX16_9FUSO Alcohol dehydrogenase, class IV EC=1.1.1.244 492 e-137 B2E5E5.1 B2E5E5_STRPN NAD-dependent methanol dehydrogenase (Medh... 458 e-127 A5M1V5.1 A5M1V5_STRPN Alcohol dehydrogenase, iron-containing 458 e-127 B1S264.1 B1S264_STRPN NAD-dependent methanol dehydrogenase (Medh... 457 e-127 >A0AG51.1 A0AG51_LISW6 Complete genome EC=1.1.1.1 Length = 383 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 250/346 (72%), Positives = 291/346 (84%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDKFL S I K++A L +AG+ ++++D+VKPNPTC+NV+ GLAFL + CD++IS+GG Sbjct: 38 TDKFLSSSPILDKVIAPLKKAGVAFSIFDDVKPNPTCENVNQGLAFLKKENCDFIISVGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG ++DYEGVH+SK K LP+VA+NTTAGTSAE+TINYVITDE+R Sbjct: 98 GSPQDAASGISILATNGGDMRDYEGVHQSKKKGLPVVAINTTAGTSAEITINYVITDEDR 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 VKMV VDKNS+A ISVNDPELM+DKPAALTAATGMDALTHA+EALVTPGAYPVTDATA+ Sbjct: 158 GVKMVCVDKNSLARISVNDPELMVDKPAALTAATGMDALTHAVEALVTPGAYPVTDATAV 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 AAVELIF L R VK+GHDIEAREQMVYAI +VHAMAHQLGGVYDLPH Sbjct: 218 AAVELIFNYLPRAVKDGHDIEAREQMVYAIFLGGMAFNNAGLGFVHAMAHQLGGVYDLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNAMLLP VE ENAK VPEKFR IAKAIGL + KS+ ECAD+ I+ I+ LS EVGIP Sbjct: 278 GVCNAMLLPIVERENAKRVPEKFRKIAKAIGLDVTNKSNQECADYAIKKIRELSIEVGIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KLSELGVTEVDL+ LA+NS+KDACAPGNPF+PTKEE I +F++IL Sbjct: 338 EKLSELGVTEVDLNKLADNSLKDACAPGNPFMPTKEETIAMFKEIL 383 >C4BX16.1 C4BX16_9FUSO Alcohol dehydrogenase, class IV EC=1.1.1.244 Length = 383 Score = 492 bits (1267), Expect = e-137, Method: Compositional matrix adjust. Identities = 239/346 (69%), Positives = 283/346 (81%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDKFL S I K+ A+LD++GI+Y ++D+VKPNPTC+NV++GL L + CD +IS+GG Sbjct: 38 TDKFLSSSKIIEKVKAILDKSGIEYVIFDDVKPNPTCENVNNGLEMLKKEKCDCIISVGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQDTA I I+A NGG +KDYEGV+KS+ P++AVNTTAGTSAE+TINYVITDEER Sbjct: 98 GSPQDTASGISILAANGGEMKDYEGVNKSEKTGFPVIAVNTTAGTSAELTINYVITDEER 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 VKM+ +DKNS+A +SVNDPELM+DKPA+LTAATGMDALTHAIEALVTPGAYPVTDA AL Sbjct: 158 HVKMICIDKNSLAKMSVNDPELMLDKPASLTAATGMDALTHAIEALVTPGAYPVTDAMAL 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 +AV++IF L R VKNG+DIEAREQMVYAI +VHAMAHQLGG+YDLPH Sbjct: 218 SAVKIIFEYLPRAVKNGNDIEAREQMVYAIFLTGMAFSNAGLGFVHAMAHQLGGLYDLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNAMLLP VEE NAK+VPEKFR IA+ IGL + KSD ECA+FVI SI+ LSK V IP Sbjct: 278 GVCNAMLLPTVEEANAKYVPEKFRPIAEYIGLDTKNKSDDECAEFVISSIRELSKTVEIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 L+ELGVT+ DLDLLAENS+KDACA NP++P KEE I LF+KIL Sbjct: 338 ETLTELGVTDPDLDLLAENSLKDACAFANPYLPDKEETIELFKKIL 383 >B2E5E5.1 B2E5E5_STRPN NAD-dependent methanol dehydrogenase (Medh) EC=1.1.1.244 Length = 383 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/348 (64%), Positives = 269/348 (77%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K+L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP ALTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V+NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 >A5M1V5.1 A5M1V5_STRPN Alcohol dehydrogenase, iron-containing Length = 383 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/348 (64%), Positives = 269/348 (77%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K+L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP ALTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V+NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 >B1S264.1 B1S264_STRPN NAD-dependent methanol dehydrogenase (Medh) EC=1.1.1.244 Length = 383 Score = 457 bits (1177), Expect = e-127, Method: Compositional matrix adjust. Identities = 223/348 (64%), Positives = 268/348 (77%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K+L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP ALTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,375,750,572 Number of extensions: 92817334 Number of successful extensions: 273137 Number of sequences better than 1.0e-05: 3765 Number of HSP's gapped: 264050 Number of HSP's successfully gapped: 3766 Length of query: 425 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 287 Effective length of database: 1,760,596,133 Effective search space: 505291090171 Effective search space used: 505291090171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)