BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0045.p (340 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1KU52.1 B1KU52_CLOBM Radical SAM domain protein 385 e-105 A0PYW3.1 A0PYW3_CLONN Biotin synthase 385 e-105 C3KWD5.1 C3KWD5_CLOB6 Radical SAM domain protein 379 e-103 B1QMG5.1 B1QMG5_CLOBO Radical SAM domain protein 379 e-103 A7FZE1.1 A7FZE1_CLOB1 Radical SAM domain protein 366 1e-99 >B1KU52.1 B1KU52_CLOBM Radical SAM domain protein Length = 348 Score = 385 bits (989), Expect = e-105, Method: Compositional matrix adjust. Identities = 177/330 (53%), Positives = 240/330 (72%) Query: 1 VSQQNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRS 60 S L L+++++ ++ L AH+ + Y VYLRGLIEF+N CK CLYCG+ Sbjct: 14 TSNDKLLYLLNNIDEENKKYLIYKAHETSLKYYSNKVYLRGLIEFTNYCKNGCLYCGISC 73 Query: 61 GNRTLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAA 120 N RYRL EEIL+CC +GY+LGYRTFVLQ GED +YT DK++ ++ S+K FPD A Sbjct: 74 QNNNAERYRLSKEEILNCCSKGYSLGYRTFVLQGGEDPYYTDDKIIDIVKSIKNLFPDCA 133 Query: 121 LTLSVGERDDESYTRLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGY 180 +TLS+GE+D + Y + Y AG DR+LLRHETAS +LY LHP M + + IGY Sbjct: 134 ITLSIGEKDYDIYKKFYDAGVDRYLLRHETASENLYEKLHPDMSFENRRRCLKNLKEIGY 193 Query: 181 QVGAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDM 240 QVGAGFM+GLPGQ+ +L+EL+P M+GIGPF+PH+DT L+++ GT+E+T + Sbjct: 194 QVGAGFMIGLPGQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLKNQSAGTLEDTTTL 253 Query: 241 IALARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICT 300 +AL RL++PD L+PATTA+GT +P GRE+ L+AGANV+MPNLSP +VR KYMLY+NKICT Sbjct: 254 LALIRLLLPDVLLPATTALGTINPMGREKGLKAGANVVMPNLSPTNVRNKYMLYDNKICT 313 Query: 301 GDESAQCRFCLEQRVKSAGFHVDMGRGDSL 330 GDE+A+CR C+E R+ +AGF +D+ RGD++ Sbjct: 314 GDEAAECRKCIENRINNAGFSLDLTRGDNI 343 >A0PYW3.1 A0PYW3_CLONN Biotin synthase Length = 348 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 174/329 (52%), Positives = 240/329 (72%) Query: 1 VSQQNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRS 60 +S + L L+++++ + L A++ R YG V++RGLIEF+N CK C YCG+R Sbjct: 14 LSGEELLYLLNNIDDENKEYLKAKANETRFKYYGDRVFMRGLIEFTNYCKNTCTYCGIRV 73 Query: 61 GNRTLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAA 120 N+ + RYRL E+IL+CC EGY LGYRTFVLQ GED ++T DK+V+++ ++K++F D A Sbjct: 74 FNKKVDRYRLSLEDILNCCSEGYRLGYRTFVLQGGEDPYFTDDKIVEIVKNIKEKFKDCA 133 Query: 121 LTLSVGERDDESYTRLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGY 180 +TLS+GE+ ESY + Y AGADR+LLRHETAS +LY LHP M + + IGY Sbjct: 134 VTLSIGEKSYESYKKYYDAGADRYLLRHETASKTLYEKLHPGMSFENRRRCLRDLKEIGY 193 Query: 181 QVGAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDM 240 QVGAGFM+G+PGQ+ +L+EL+P M+GIGPF+P DTPL +E+ GTVE T+ M Sbjct: 194 QVGAGFMIGIPGQTNEDFVKDLLFLKELEPHMVGIGPFIPQCDTPLGNEKGGTVEVTVLM 253 Query: 241 IALARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICT 300 +++ RL++P L+P+TTA+GT HP GRE L+AGANV+MPNLSP VR KY LY+ KICT Sbjct: 254 LSIIRLLLPQVLLPSTTALGTIHPMGREMGLKAGANVVMPNLSPTCVREKYALYDGKICT 313 Query: 301 GDESAQCRFCLEQRVKSAGFHVDMGRGDS 329 GDE+A+CR C++ R++SAGF +DM RGD+ Sbjct: 314 GDEAAECRMCIQNRIESAGFTLDMSRGDN 342 >C3KWD5.1 C3KWD5_CLOB6 Radical SAM domain protein Length = 348 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 171/306 (55%), Positives = 231/306 (75%) Query: 25 AHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEILDCCREGYT 84 +H+ R+ Y VYLRGLIEF+N CK +CLYCG+ N RYRL EEIL CC +GY+ Sbjct: 38 SHETRLKHYNNKVYLRGLIEFTNYCKNSCLYCGISCRNNNAERYRLSKEEILKCCSKGYS 97 Query: 85 LGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYTRLYAAGADRF 144 LGYRTFVLQ GED +YT DK++ ++ S+K+ FPD A+TLS+GE+ E+Y + Y AGADR+ Sbjct: 98 LGYRTFVLQGGEDPYYTDDKIINIVKSIKKLFPDCAITLSIGEKSYETYRKFYDAGADRY 157 Query: 145 LLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQSXXXXXXXXXY 204 LLRHETAS +LY LHP M + ++IGYQVGAGFM+GLP Q+ + Sbjct: 158 LLRHETASKNLYEKLHPDMSFENRRSCLENLKKIGYQVGAGFMIGLPNQTNEDYVNDLIF 217 Query: 205 LQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALIPATTAMGTAHP 264 L+EL+P M+GIGPF+PH++T L+++ GT+E+T ++AL RL++PD L+PATTA+GT +P Sbjct: 218 LKELKPHMVGIGPFIPHKNTILKNQSAGTLEDTTTLLALIRLLLPDVLLPATTALGTINP 277 Query: 265 NGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQRVKSAGFHVDM 324 GRE L+AGANV+MPNLSP +VR KYMLY+NKICTGDE+A+CR C+E R+ +AGF +++ Sbjct: 278 MGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICTGDEAAECRKCIENRINNAGFSLNL 337 Query: 325 GRGDSL 330 RGD++ Sbjct: 338 TRGDNI 343 >B1QMG5.1 B1QMG5_CLOBO Radical SAM domain protein Length = 348 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 171/306 (55%), Positives = 231/306 (75%) Query: 25 AHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEILDCCREGYT 84 +H+ R+ Y VYLRGLIEF+N CK +CLYCG+ N RYRL EEIL CC +GY+ Sbjct: 38 SHETRLKHYNNKVYLRGLIEFTNYCKNSCLYCGISCRNNNAERYRLSKEEILKCCSKGYS 97 Query: 85 LGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYTRLYAAGADRF 144 LGYRTFVLQ GED +YT DK++ ++ S+K+ FPD A+TLS+GE+ E+Y + Y AGADR+ Sbjct: 98 LGYRTFVLQGGEDPYYTDDKIINIVKSIKKLFPDCAITLSIGEKSYETYRKFYDAGADRY 157 Query: 145 LLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQSXXXXXXXXXY 204 LLRHETAS +LY LHP M + ++IGYQVGAGFM+GLP Q+ + Sbjct: 158 LLRHETASKNLYEKLHPDMSFENRRSCLENLKKIGYQVGAGFMIGLPNQTNEDYVNDLIF 217 Query: 205 LQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALIPATTAMGTAHP 264 L+EL+P M+GIGPF+PH++T L+++ GT+E+T ++AL RL++PD L+PATTA+GT +P Sbjct: 218 LKELKPHMVGIGPFIPHKNTILKNQSAGTLEDTTTLLALIRLLLPDVLLPATTALGTINP 277 Query: 265 NGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQRVKSAGFHVDM 324 GRE L+AGANV+MPNLSP +VR KYMLY+NKICTGDE+A+CR C+E R+ +AGF +++ Sbjct: 278 MGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICTGDEAAECRKCIENRINNAGFSLNL 337 Query: 325 GRGDSL 330 RGD++ Sbjct: 338 TRGDNI 343 >A7FZE1.1 A7FZE1_CLOB1 Radical SAM domain protein Length = 348 Score = 366 bits (940), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 175/330 (53%), Positives = 243/330 (73%) Query: 1 VSQQNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRS 60 S L L+++++ ++ L AH+ + Y + VYLRGLIEF+N CK CLYCG+ Sbjct: 14 TSNDKLLYLLNNIDKENKKYLIYKAHETSLKHYNSKVYLRGLIEFTNYCKNACLYCGISC 73 Query: 61 GNRTLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAA 120 N RYRL EEIL+CC++GY+LGYRTFVLQ GED +YT +K++ +I S+K+ FP+ A Sbjct: 74 NNNNAERYRLSEEEILNCCKKGYSLGYRTFVLQGGEDPYYTDNKIIDIIKSIKKLFPNCA 133 Query: 121 LTLSVGERDDESYTRLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGY 180 +TLS+GE+ E+Y + Y AGADR+LLRHETAS +LY LHP M + ++IGY Sbjct: 134 VTLSIGEKSYETYKKFYDAGADRYLLRHETASKNLYEKLHPDMSFENRHSCLRNLKKIGY 193 Query: 181 QVGAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDM 240 QVGAGFM+GLP Q+ +L+EL+P M+GIGPF+PH+DT L+++ GT+E+T + Sbjct: 194 QVGAGFMIGLPSQTNEDYVNDLIFLKELKPHMVGIGPFIPHKDTVLKNQSAGTLEDTTTL 253 Query: 241 IALARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICT 300 +AL RL++P+ L+PATTA+GT +P GRE L+AGANV+MPNLSP +VR KYMLY+NKICT Sbjct: 254 LALIRLLLPEVLLPATTALGTINPMGREAGLKAGANVVMPNLSPTNVRDKYMLYDNKICT 313 Query: 301 GDESAQCRFCLEQRVKSAGFHVDMGRGDSL 330 GDE+A+CR C+E R+ +AGF +D+ RGD++ Sbjct: 314 GDEAAECRKCIENRINNAGFSLDLTRGDNI 343 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,870,930,116 Number of extensions: 72212885 Number of successful extensions: 152351 Number of sequences better than 1.0e-05: 525 Number of HSP's gapped: 152375 Number of HSP's successfully gapped: 526 Length of query: 340 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 204 Effective length of database: 1,779,438,163 Effective search space: 363005385252 Effective search space used: 363005385252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)