BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0042.p (484 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E3M7.1 B9E3M7_CLOK1 Putative uncharacterized protein 560 e-157 A5MZP7.1 A5MZP7_CLOK5 AspA2 EC=4.3.1.1 560 e-157 Q97II9.1 Q97II9_CLOAB Aspartate ammonia-lyase 551 e-155 A6LYE3.1 A6LYE3_CLOB8 Fumarate lyase 539 e-151 B0KBX7.1 B0KBX7_THEP3 Fumarate lyase 519 e-145 >B9E3M7.1 B9E3M7_CLOK1 Putative uncharacterized protein Length = 465 Score = 560 bits (1444), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/461 (56%), Positives = 347/461 (75%) Query: 8 RMERDALGSLPVPQDAYYGIHTARAKENFAAGGRPANRKLMHAIARVKQAAAKVNGRNHA 67 R+E+D LG + +Y+GI+T RA ENF GG+ N L+ IA +K+AAA VN Sbjct: 4 RIEKDLLGEKEIDNSSYFGINTKRALENFNLGGKTVNLNLIKEIALIKKAAAIVNEDLKE 63 Query: 68 LPQQKVQAILAACEEILDGCLDAHLIVDAYQGGAGTSSNMNVNEVIANRAIELLGGSKGD 127 LP+ K +AI+ A EE+++G + + A+QGGAGTS+NMNVNEVIANRAIE+LGG KGD Sbjct: 64 LPEDKAEAIIKASEEVMEGNFNDQFFLSAFQGGAGTSTNMNVNEVIANRAIEILGGKKGD 123 Query: 128 YSIVHPIDDVNLHQSTNDVYPTALRIAVIPLLRQLAEELASLQEVFQVKEHEYADLLRLG 187 Y++VHP++DVN+ QSTNDVYPTALRI+ I +R+L+ LA LQE QVKE+E++D+++LG Sbjct: 124 YNLVHPLNDVNMSQSTNDVYPTALRISAIRSIRRLSSCLADLQEELQVKENEFSDIIKLG 183 Query: 188 RTQLMDALPILAGQSFGAYAKAAARDRWRLYKAEERLREINIGGTAVGTGMNAPLTYIYT 247 RTQLMDALP++ GQ FGAYAKA ARDRWR+YK EERLRE+NIGGTA+GTG+NA +IY Sbjct: 184 RTQLMDALPMMVGQGFGAYAKAIARDRWRIYKVEERLREVNIGGTAIGTGLNATNKFIYE 243 Query: 248 IADELRELTGLGLCRSDFPMDGTQNMDVFVEVSGLLKAAAVNLLKISNDFRLLSSGPAGG 307 I D L++LTGLG+ RSD+PMD TQN D+FVEVSGLLK+ AVNLLKISND R+L+SGP GG Sbjct: 244 ITDVLQDLTGLGIARSDYPMDVTQNCDIFVEVSGLLKSLAVNLLKISNDLRILNSGPVGG 303 Query: 308 IGEYILPAVQAGSSIMPGKVNPVIAEMLGSVAMRVIADDTAVTLAAASGQLELNAFTPVX 367 IGE ILP VQ+GS+IMPGKVNPVIAEM V++RVI++DTA+T+A++SGQLELNAFTP+ Sbjct: 304 IGEIILPKVQSGSTIMPGKVNPVIAEMTAQVSIRVISNDTAITMASSSGQLELNAFTPLI 363 Query: 368 XXXXXXXXXXXXHGIPLFRERCLSGLKLDEHRCREHVDRSGVLAAATITYLGYDNXXXXX 427 + +FRE+C+ G+K++E CR++++ S LA A + Y+GYD Sbjct: 364 AECLLESLELLEKTVIIFREKCIKGIKVNEENCRKNLEASTALATALVHYIGYDKAGKLA 423 Query: 428 XXXXXSGRPLEQLLVEGGWMTEKQIQEVLHPLEVTKPGIPG 468 + + ++L E + ++I E+++P ++TKPGIPG Sbjct: 424 EKALKEHKTIREILYEEEVLPREKIDEIINPYQLTKPGIPG 464 >A5MZP7.1 A5MZP7_CLOK5 AspA2 EC=4.3.1.1 Length = 465 Score = 560 bits (1444), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/461 (56%), Positives = 347/461 (75%) Query: 8 RMERDALGSLPVPQDAYYGIHTARAKENFAAGGRPANRKLMHAIARVKQAAAKVNGRNHA 67 R+E+D LG + +Y+GI+T RA ENF GG+ N L+ IA +K+AAA VN Sbjct: 4 RIEKDLLGEKEIDNSSYFGINTKRALENFNLGGKTVNLNLIKEIALIKKAAAIVNEDLKE 63 Query: 68 LPQQKVQAILAACEEILDGCLDAHLIVDAYQGGAGTSSNMNVNEVIANRAIELLGGSKGD 127 LP+ K +AI+ A EE+++G + + A+QGGAGTS+NMNVNEVIANRAIE+LGG KGD Sbjct: 64 LPEDKAEAIIKASEEVMEGNFNDQFFLSAFQGGAGTSTNMNVNEVIANRAIEILGGKKGD 123 Query: 128 YSIVHPIDDVNLHQSTNDVYPTALRIAVIPLLRQLAEELASLQEVFQVKEHEYADLLRLG 187 Y++VHP++DVN+ QSTNDVYPTALRI+ I +R+L+ LA LQE QVKE+E++D+++LG Sbjct: 124 YNLVHPLNDVNMSQSTNDVYPTALRISAIRSIRRLSSCLADLQEELQVKENEFSDIIKLG 183 Query: 188 RTQLMDALPILAGQSFGAYAKAAARDRWRLYKAEERLREINIGGTAVGTGMNAPLTYIYT 247 RTQLMDALP++ GQ FGAYAKA ARDRWR+YK EERLRE+NIGGTA+GTG+NA +IY Sbjct: 184 RTQLMDALPMMVGQGFGAYAKAIARDRWRIYKVEERLREVNIGGTAIGTGLNATNKFIYE 243 Query: 248 IADELRELTGLGLCRSDFPMDGTQNMDVFVEVSGLLKAAAVNLLKISNDFRLLSSGPAGG 307 I D L++LTGLG+ RSD+PMD TQN D+FVEVSGLLK+ AVNLLKISND R+L+SGP GG Sbjct: 244 ITDVLQDLTGLGIARSDYPMDVTQNCDIFVEVSGLLKSLAVNLLKISNDLRILNSGPVGG 303 Query: 308 IGEYILPAVQAGSSIMPGKVNPVIAEMLGSVAMRVIADDTAVTLAAASGQLELNAFTPVX 367 IGE ILP VQ+GS+IMPGKVNPVIAEM V++RVI++DTA+T+A++SGQLELNAFTP+ Sbjct: 304 IGEIILPKVQSGSTIMPGKVNPVIAEMTAQVSIRVISNDTAITMASSSGQLELNAFTPLI 363 Query: 368 XXXXXXXXXXXXHGIPLFRERCLSGLKLDEHRCREHVDRSGVLAAATITYLGYDNXXXXX 427 + +FRE+C+ G+K++E CR++++ S LA A + Y+GYD Sbjct: 364 AECLLESLELLEKTVIIFREKCIKGIKVNEENCRKNLEASTALATALVHYIGYDKAGKLA 423 Query: 428 XXXXXSGRPLEQLLVEGGWMTEKQIQEVLHPLEVTKPGIPG 468 + + ++L E + ++I E+++P ++TKPGIPG Sbjct: 424 EKALKEHKTIREILYEEEVLPREKIDEIINPYQLTKPGIPG 464 >Q97II9.1 Q97II9_CLOAB Aspartate ammonia-lyase Length = 470 Score = 551 bits (1421), Expect = e-155, Method: Compositional matrix adjust. Identities = 261/464 (56%), Positives = 350/464 (75%) Query: 5 SAYRMERDALGSLPVPQDAYYGIHTARAKENFAAGGRPANRKLMHAIARVKQAAAKVNGR 64 S +R+E+D LG +P+++YYGI++ RA ENF + N L++A+ VK+AAA + + Sbjct: 6 SDFRIEKDLLGEKNLPKESYYGINSLRAFENFNISNKKVNLNLIYAVVIVKKAAALAHKK 65 Query: 65 NHALPQQKVQAILAACEEILDGCLDAHLIVDAYQGGAGTSSNMNVNEVIANRAIELLGGS 124 L +K AI++AC++IL G D I ++ QGGAGTS+NMNVNEVIANRAIELL GS Sbjct: 66 LKELKDEKADAIISACDDILSGKFDDEFITNSLQGGAGTSTNMNVNEVIANRAIELLNGS 125 Query: 125 KGDYSIVHPIDDVNLHQSTNDVYPTALRIAVIPLLRQLAEELASLQEVFQVKEHEYADLL 184 KGDYSI+HPI+DVN+ QSTNDVYPTALRIA I LLR L++ L+ LQE FQ+KE++++D+L Sbjct: 126 KGDYSIIHPIEDVNMSQSTNDVYPTALRIAAIKLLRPLSDALSKLQESFQIKENDFSDIL 185 Query: 185 RLGRTQLMDALPILAGQSFGAYAKAAARDRWRLYKAEERLREINIGGTAVGTGMNAPLTY 244 LGRT+LMDALP++ GQ FGAYAKA ARDRWR+YK EERLREINIGG A+GTG+NA Y Sbjct: 186 MLGRTELMDALPMMVGQGFGAYAKAFARDRWRIYKVEERLREINIGGNAIGTGINASYDY 245 Query: 245 IYTIADELRELTGLGLCRSDFPMDGTQNMDVFVEVSGLLKAAAVNLLKISNDFRLLSSGP 304 I+T+ D L++LTGLGL RSD+PMD TQNMDVFVEVSGLLK+ AVNL+KISND RLL+SGP Sbjct: 246 IFTVTDILQDLTGLGLARSDYPMDVTQNMDVFVEVSGLLKSCAVNLIKISNDIRLLNSGP 305 Query: 305 AGGIGEYILPAVQAGSSIMPGKVNPVIAEMLGSVAMRVIADDTAVTLAAASGQLELNAFT 364 GGIGE+I+ VQAGSSIMPGKVNPVI EM +M+V+A+DTA++LA++ GQL+LNAF+ Sbjct: 306 VGGIGEFIVEEVQAGSSIMPGKVNPVICEMTAQTSMKVMANDTAISLASSFGQLQLNAFS 365 Query: 365 PVXXXXXXXXXXXXXHGIPLFRERCLSGLKLDEHRCREHVDRSGVLAAATITYLGYDNXX 424 P+ + + +FRE+C+ +K++E +C E+++ S L A + Y+GYD Sbjct: 366 PLIAESLLESLEILTNAVNVFREKCIDSIKINEEKCLENLEASTALVTALVHYIGYDKAS 425 Query: 425 XXXXXXXXSGRPLEQLLVEGGWMTEKQIQEVLHPLEVTKPGIPG 468 + + +LL+ +T+++I ++L+P E+TKPGIPG Sbjct: 426 IIAKKALKEKKSIRELLLLEKILTKEEIDKILNPYEITKPGIPG 469 >A6LYE3.1 A6LYE3_CLOB8 Fumarate lyase Length = 465 Score = 539 bits (1389), Expect = e-151, Method: Compositional matrix adjust. Identities = 251/463 (54%), Positives = 340/463 (73%) Query: 7 YRMERDALGSLPVPQDAYYGIHTARAKENFAAGGRPANRKLMHAIARVKQAAAKVNGRNH 66 YR+E+D LG + Y GI+TARA ENF + N L+ IA +K+AAA N Sbjct: 3 YRLEQDVLGEKNIDNSTYSGINTARALENFDLNSKSVNLNLIREIALIKKAAAMTNKTLK 62 Query: 67 ALPQQKVQAILAACEEILDGCLDAHLIVDAYQGGAGTSSNMNVNEVIANRAIELLGGSKG 126 L +K AI+ A EE++DG D + A+QGGAGTS+NMNVNEVIANRAIELLGG KG Sbjct: 63 FLAPEKADAIIKASEEVIDGKFDYEFKLSAFQGGAGTSTNMNVNEVIANRAIELLGGVKG 122 Query: 127 DYSIVHPIDDVNLHQSTNDVYPTALRIAVIPLLRQLAEELASLQEVFQVKEHEYADLLRL 186 DY++VHP++ VN+ QSTNDVYP+ALRIA I L+R+L++ L+SLQE Q+KE+E++D+++L Sbjct: 123 DYNLVHPLNHVNMSQSTNDVYPSALRIAAIRLIRKLSDSLSSLQEALQIKENEFSDIIKL 182 Query: 187 GRTQLMDALPILAGQSFGAYAKAAARDRWRLYKAEERLREINIGGTAVGTGMNAPLTYIY 246 GRTQLMDALP+ AGQSFGAY+KA RDRWR+YK EERLR+IN+GGTA+GTG+NA Y++ Sbjct: 183 GRTQLMDALPMTAGQSFGAYSKAIERDRWRIYKVEERLRQINLGGTAIGTGLNATNKYVF 242 Query: 247 TIADELRELTGLGLCRSDFPMDGTQNMDVFVEVSGLLKAAAVNLLKISNDFRLLSSGPAG 306 + D ++ LTGLG+ RSD+PMD TQN D+FVE SGLLK+ +VNLLKISND RLL+SGP G Sbjct: 243 MMTDIIQNLTGLGIARSDYPMDITQNCDIFVETSGLLKSCSVNLLKISNDLRLLNSGPRG 302 Query: 307 GIGEYILPAVQAGSSIMPGKVNPVIAEMLGSVAMRVIADDTAVTLAAASGQLELNAFTPV 366 GIGE ILP +QAGS+IMPGKVNPVI EM+ V++RVI++D+A+T+A++ GQLELNAFTP+ Sbjct: 303 GIGEVILPKMQAGSTIMPGKVNPVIPEMVAQVSLRVISNDSAITMASSMGQLELNAFTPL 362 Query: 367 XXXXXXXXXXXXXHGIPLFRERCLSGLKLDEHRCREHVDRSGVLAAATITYLGYDNXXXX 426 + LF+++C++ LK+DE RC E+++ S V A + + +LGYD Sbjct: 363 IAECLLESLELLERSVTLFKDKCINDLKMDEDRCLENLENSLVSATSLVPHLGYDKASLI 422 Query: 427 XXXXXXSGRPLEQLLVEGGWMTEKQIQEVLHPLEVTKPGIPGR 469 +G+ + ++L+E + E I ++L P E+ + IPG+ Sbjct: 423 SKKALATGKTIREILLEEQILPEDIIDKILSPTELIRTHIPGK 465 >B0KBX7.1 B0KBX7_THEP3 Fumarate lyase Length = 472 Score = 519 bits (1336), Expect = e-145, Method: Compositional matrix adjust. Identities = 253/468 (54%), Positives = 335/468 (71%) Query: 7 YRMERDALGSLPVPQDAYYGIHTARAKENFAAGGRPANRKLMHAIARVKQAAAKVNGRNH 66 YR+E D LG VP DAYYGIHT RA ENF G+ +R+L+ ++A VK+AAA N + Sbjct: 3 YRIEHDLLGQKEVPVDAYYGIHTLRAYENFNISGQKIHRELIISLAMVKKAAAIANMKAG 62 Query: 67 ALPQQKVQAILAACEEILDGCLDAHLIVDAYQGGAGTSSNMNVNEVIANRAIELLGGSKG 126 L +++ +AI+ AC+EI+ G IVDA QGGAGTSSNMN NEVIANRAIELLGGSKG Sbjct: 63 LLDEKRAKAIILACDEIISGKYHDQFIVDALQGGAGTSSNMNANEVIANRAIELLGGSKG 122 Query: 127 DYSIVHPIDDVNLHQSTNDVYPTALRIAVIPLLRQLAEELASLQEVFQVKEHEYADLLRL 186 DYSIVHP++ VNL QSTNDV+PTA+RIA I LL+ L+E+LA LQ V Q KE E++D+ ++ Sbjct: 123 DYSIVHPLEHVNLSQSTNDVFPTAVRIAAIRLLKPLSEDLAELQSVLQEKEEEFSDVCKM 182 Query: 187 GRTQLMDALPILAGQSFGAYAKAAARDRWRLYKAEERLREINIGGTAVGTGMNAPLTYIY 246 GRT+L DALP++ GQ FGAYA+A ARDRWR+YK EERLR++NIGGTAVGTG+NA L YI+ Sbjct: 183 GRTELQDALPVMLGQEFGAYAQAVARDRWRIYKVEERLRQVNIGGTAVGTGVNAELKYIF 242 Query: 247 TIADELRELTGLGLCRSDFPMDGTQNMDVFVEVSGLLKAAAVNLLKISNDFRLLSSGPAG 306 ++ + LRE+TGLGL R+++ MD TQN DVFVEVSGLLKAAAVNL+KISND RL+SSGP Sbjct: 243 SVIEILREITGLGLARAEYMMDPTQNNDVFVEVSGLLKAAAVNLIKISNDIRLMSSGPKC 302 Query: 307 GIGEYILPAVQAGSSIMPGKVNPVIAEMLGSVAMRVIADDTAVTLAAASGQLELNAFTPV 366 G+ E +P VQ+GSSIMPGKVNPVI E + A +VIA D A+TL A +G+ ELN P Sbjct: 303 GLMEINIPEVQSGSSIMPGKVNPVIPEAVKQAAFQVIACDHAITLCAQAGEFELNFTLPQ 362 Query: 367 XXXXXXXXXXXXXHGIPLFRERCLSGLKLDEHRCREHVDRSGVLAAATITYLGYDNXXXX 426 + I +F ++C+ G+ ++ +E +++S A A + Y+GY+ Sbjct: 363 IAYNLFMQIGLLRNAIRIFIDKCIKGITANKDYLKETIEKSYSYATALVPYIGYEKATEI 422 Query: 427 XXXXXXSGRPLEQLLVEGGWMTEKQIQEVLHPLEVTKPGIPGRSGQSG 474 G+ + ++++E G TE+++ + +P E+TKPGIPG+ G Sbjct: 423 AKMAKEKGKTIREVVLEAGLFTEEELDIIFNPYEMTKPGIPGKRRLKG 470 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,768,395,572 Number of extensions: 112965412 Number of successful extensions: 275269 Number of sequences better than 1.0e-05: 2588 Number of HSP's gapped: 271801 Number of HSP's successfully gapped: 2592 Length of query: 484 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 344 Effective length of database: 1,741,754,103 Effective search space: 599163411432 Effective search space used: 599163411432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)