BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0041.p (357 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K7M8.1 B3K7M8_9BACI Molybdenum cofactor biosynthesis protein A 355 3e-96 Q4ERS9.1 Q4ERS9_LISMO Molybdenum cofactor biosynthesis protein A 328 4e-88 Q8Y870.1 MOAA_LISMO Molybdenum cofactor biosynthesis protein A 328 4e-88 Q4EI22.1 Q4EI22_LISMO Molybdenum cofactor biosynthesis protein A 327 1e-87 Q721B9.1 MOAA_LISMF Molybdenum cofactor biosynthesis protein A 327 1e-87 >B3K7M8.1 B3K7M8_9BACI Molybdenum cofactor biosynthesis protein A Length = 334 Score = 355 bits (912), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 180/352 (51%), Positives = 242/352 (68%), Gaps = 22/352 (6%) Query: 8 QALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSP--EPLTFDEIATVVSVLADM 65 + L DSFGR+H+Y+RISVTDRCNL C YC P++ F P + ++++EIA+++ VLA M Sbjct: 3 EPLKDSFGRVHDYIRISVTDRCNLRCVYCMPEEG-MEFQPHDQIMSYEEIASIMRVLAPM 61 Query: 66 GVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNI 125 GV+KVRLTGGEPL+R LE LV ++ ++ G++ I LTTNGI+L SKAR+L+EAGLT +NI Sbjct: 62 GVSKVRLTGGEPLVRKDLETLVHQIASIEGVQDISLTTNGIMLPSKARLLKEAGLTRINI 121 Query: 126 SLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTL 185 SLDSLQ ER+ARIT GG V +VL I ++ AG +K+NMV+MKG N+DEI F+ LTL Sbjct: 122 SLDSLQEERYARITRGGRVHKVLEGIEAAYEAGLNPIKLNMVLMKGFNEDEIRDFIALTL 181 Query: 186 KYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDV 245 PL +RFIEYMPIG+ ++ W+ Y+PL + E C AGWA + ++ G Sbjct: 182 DRPLHVRFIEYMPIGQASDTWRDSYLPLSRVSEVCQEAGWAIQ----EEQG--------- 228 Query: 246 TMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVR 305 + +GP+R ++ GA G FGLI PVS HFC +CNRLR+TA GHIK CLYW+DE+NVR Sbjct: 229 ---PSGNGPSRNMKVVGATGTFGLIHPVSDHFCDSCNRLRLTADGHIKACLYWSDEFNVR 285 Query: 306 PYIGEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQENTVRQMSQIGG 357 I + + LF ++L KP H+M L++ TVR+MSQIGG Sbjct: 286 RVIDDPSAVANLFRKALGAKPLNHEMALALEKKA---QSHTPTVRRMSQIGG 334 >Q4ERS9.1 Q4ERS9_LISMO Molybdenum cofactor biosynthesis protein A Length = 333 Score = 328 bits (841), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 170/354 (48%), Positives = 226/354 (63%), Gaps = 26/354 (7%) Query: 8 QALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEP--LTFDEIATVVSVLADM 65 Q L D FGR+H+Y+RISVTDRCNL C YC P++ F P L+ DEI + + ++ Sbjct: 2 QLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEG-LTFLPHEKVLSKDEIVSFMELMVKF 60 Query: 66 GVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNI 125 G+ KVR+TGGEPLLR + E+V L A+ IE I +TTN + LA KA L+EAGLT VNI Sbjct: 61 GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNI 120 Query: 126 SLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTL 185 SLDSL +RF IT GG + +VL I + G +K+N+V++KG NDDEI FL+ T Sbjct: 121 SLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK 180 Query: 186 KYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDV 245 ++IRFIEYMPIG W+ Y+PL I E C+ AG+ YEPV S Sbjct: 181 DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDS------------- 227 Query: 246 TMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVR 305 +GP+ +RIKGA G FG+I PVS HFC +CNRLR+TA G+IK CLYW++E N+R Sbjct: 228 ---IRGNGPSENFRIKGAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIR 284 Query: 306 PYIGEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQEN--TVRQMSQIGG 357 P+I + L QL +++++KP H+M +L++ VQ N T R+MSQIGG Sbjct: 285 PFIQDPVKLMQLVQKAIDNKPENHEMALKLQDE-----VQSNKPTWRRMSQIGG 333 >Q8Y870.1 MOAA_LISMO Molybdenum cofactor biosynthesis protein A Length = 333 Score = 328 bits (841), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 170/354 (48%), Positives = 226/354 (63%), Gaps = 26/354 (7%) Query: 8 QALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEP--LTFDEIATVVSVLADM 65 Q L D FGR+H+Y+RISVTDRCNL C YC P++ F P L+ DEI + + ++ Sbjct: 2 QLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEG-LTFLPHEKVLSKDEIVSFMELMVKF 60 Query: 66 GVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNI 125 G+ KVR+TGGEPLLR + E+V L A+ IE I +TTN + LA KA L+EAGLT VNI Sbjct: 61 GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKEAGLTRVNI 120 Query: 126 SLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTL 185 SLDSL +RF IT GG + +VL I + G +K+N+V++KG NDDEI FL+ T Sbjct: 121 SLDSLHADRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK 180 Query: 186 KYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDV 245 ++IRFIEYMPIG W+ Y+PL I E C+ AG+ YEPV S Sbjct: 181 DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDS------------- 227 Query: 246 TMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVR 305 +GP+ +RIKGA G FG+I PVS HFC +CNRLR+TA G+IK CLYW++E N+R Sbjct: 228 ---IRGNGPSENFRIKGAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIR 284 Query: 306 PYIGEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQEN--TVRQMSQIGG 357 P+I + L QL +++++KP H+M +L++ VQ N T R+MSQIGG Sbjct: 285 PFIQDPVKLMQLVQKAIDNKPENHEMALKLQDE-----VQSNKPTWRRMSQIGG 333 >Q4EI22.1 Q4EI22_LISMO Molybdenum cofactor biosynthesis protein A Length = 333 Score = 327 bits (838), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 169/354 (47%), Positives = 226/354 (63%), Gaps = 26/354 (7%) Query: 8 QALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEP--LTFDEIATVVSVLADM 65 Q L D FGR+H+Y+RISVTDRCNL C YC P++ F P L+ DEI + + ++ Sbjct: 2 QLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEG-LTFLPHEKVLSKDEIVSFMELMVKF 60 Query: 66 GVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNI 125 G+ KVR+TGGEPLLR + E+V L A+ IE I +TTN + LA KA L++AGLT VNI Sbjct: 61 GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNI 120 Query: 126 SLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTL 185 SLDSL +RF IT GG + +VL I + G +K+N+V++KG NDDEI FL+ T Sbjct: 121 SLDSLHEDRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK 180 Query: 186 KYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDV 245 ++IRFIEYMPIG W+ Y+PL I E C+ AG+ YEPV S Sbjct: 181 DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDS------------- 227 Query: 246 TMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVR 305 +GP+ +RIKGA G FG+I PVS HFC +CNRLR+TA G+IK CLYW++E N+R Sbjct: 228 ---IRGNGPSENFRIKGAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIR 284 Query: 306 PYIGEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQEN--TVRQMSQIGG 357 P+I + L QL +++++KP H+M +L++ VQ N T R+MSQIGG Sbjct: 285 PFIQDPVKLMQLVQKAIDNKPENHEMALKLQDE-----VQSNKPTWRRMSQIGG 333 >Q721B9.1 MOAA_LISMF Molybdenum cofactor biosynthesis protein A Length = 333 Score = 327 bits (838), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 169/354 (47%), Positives = 226/354 (63%), Gaps = 26/354 (7%) Query: 8 QALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEP--LTFDEIATVVSVLADM 65 Q L D FGR+H+Y+RISVTDRCNL C YC P++ F P L+ DEI + + ++ Sbjct: 2 QLLKDKFGRVHDYIRISVTDRCNLRCVYCMPEEG-LTFLPHEKVLSKDEIVSFMELMVKF 60 Query: 66 GVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNI 125 G+ KVR+TGGEPLLR + E+V L A+ IE I +TTN + LA KA L++AGLT VNI Sbjct: 61 GIKKVRITGGEPLLRTDIVEIVRGLGAIPEIEDISITTNAMYLAKKAEALKDAGLTRVNI 120 Query: 126 SLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTL 185 SLDSL +RF IT GG + +VL I + G +K+N+V++KG NDDEI FL+ T Sbjct: 121 SLDSLHEDRFKAITRGGRLQKVLDGIQKAEEVGLFPIKLNVVLIKGQNDDEITDFLRFTK 180 Query: 186 KYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDV 245 ++IRFIEYMPIG W+ Y+PL I E C+ AG+ YEPV S Sbjct: 181 DKDINIRFIEYMPIGHAGTSWKEKYLPLDTIFEACNEAGYEYEPVDS------------- 227 Query: 246 TMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVR 305 +GP+ +RIKGA G FG+I PVS HFC +CNRLR+TA G+IK CLYW++E N+R Sbjct: 228 ---IRGNGPSENFRIKGAKGTFGVIHPVSAHFCDSCNRLRLTADGYIKACLYWDEEMNIR 284 Query: 306 PYIGEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQEN--TVRQMSQIGG 357 P+I + L QL +++++KP H+M +L++ VQ N T R+MSQIGG Sbjct: 285 PFIQDPVKLMQLVQKAIDNKPENHEMALKLQDE-----VQSNKPTWRRMSQIGG 333 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,204,689,525 Number of extensions: 95370984 Number of successful extensions: 227415 Number of sequences better than 1.0e-05: 2086 Number of HSP's gapped: 223765 Number of HSP's successfully gapped: 2122 Length of query: 357 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 220 Effective length of database: 1,770,017,148 Effective search space: 389403772560 Effective search space used: 389403772560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)