BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0028.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WCV2.1 Q5WCV2_BACSK Unsaturated glucuronyl hydrolase 306 1e-81 A9KKJ2.1 A9KKJ2_CLOPH Glycosyl hydrolase family 88 296 1e-78 B1D7I5.1 B1D7I5_9BACL Glycosyl hydrolase family 88 291 4e-77 B3KCR7.1 B3KCR7_9BACI Glycosyl hydrolase family 88 291 6e-77 B1D7S3.1 B1D7S3_9BACL Glycosyl hydrolase family 88 237 8e-61 >Q5WCV2.1 Q5WCV2_BACSK Unsaturated glucuronyl hydrolase Length = 480 Score = 306 bits (784), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 139/240 (57%), Positives = 182/240 (75%), Gaps = 1/240 (0%) Query: 10 MDKLDTKVTRMAEQIGGKCPHYAGADGKYDDMASDWWTTGFWPGLLWIMHDMTGKELYKE 69 +D+L+ ++TRMA+Q+ + PH + + G+YDDM WWT+GF+PGLLW+MHD+TG +K Sbjct: 121 IDELEQRITRMAKQMENRSPHASDSSGRYDDMPVSWWTSGFYPGLLWLMHDVTGNAFFKN 180 Query: 70 AAWHWDATLEEWFVKPTVELHHDVGFQFLPTAVIKHTLTGDEDGLRRGIEAANFLAARYN 129 A W +EE F + + LHHDVGFQFL TAV+++ TG EDGLR G++AAN+LA R+N Sbjct: 181 QAEGWSRKIEEAFQEHPIILHHDVGFQFLLTAVMEYEQTGSEDGLRIGLKAANYLAGRFN 240 Query: 130 PAGKFIRAWN-EDKYGWVIIDCMLNISLLFWASKVTGDPRYKHIAVSHAETTMQYGIRED 188 P G FI+AWN +DK GW IIDCM+NISLL+WAS+VT DP Y IA +HA TT+Q+G+R+D Sbjct: 241 PIGGFIKAWNGDDKKGWAIIDCMMNISLLYWASRVTNDPSYGQIASAHANTTLQWGVRDD 300 Query: 189 GSTKHILSFDAETGAYIENFGGQGYSPESCWSRGAAWGLYGFFNTYRHTGDERFLNTAKR 248 GST HILSF++ TG +IE+ GGQGY +S WSRG AW LYGF N RHTG +L+ AKR Sbjct: 301 GSTSHILSFNSHTGEFIESIGGQGYGSDSAWSRGNAWALYGFANACRHTGKRAYLHAAKR 360 >A9KKJ2.1 A9KKJ2_CLOPH Glycosyl hydrolase family 88 Length = 364 Score = 296 bits (758), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 141/241 (58%), Positives = 178/241 (73%), Gaps = 2/241 (0%) Query: 8 EIMDKLDTKVTRMAEQIGGKCPHYAGADGKYDDMASDWWTTGFWPGLLWIMHDMTGKELY 67 E++ K++ K+ M E +G P Y G DG Y D W +GFWPG+LWI++D+TG+E Y Sbjct: 5 ELIQKIEAKMVFMLEHLGDCVPWY-GEDGVYHGWQEDSWVSGFWPGMLWILYDLTGRESY 63 Query: 68 KEAAWHWDATLEEWFVKPTVELHHDVGFQFLPTAVIKHTLTGDEDGLRRGIEAANFLAAR 127 K+AAW WD +E+ +++ + LHHDVGFQFLPTAV+K+ LTGDEDG RRG+ AANFLA R Sbjct: 64 KQAAWEWDGRIEQCYLRNS-NLHHDVGFQFLPTAVMKYKLTGDEDGRRRGLFAANFLAGR 122 Query: 128 YNPAGKFIRAWNEDKYGWVIIDCMLNISLLFWASKVTGDPRYKHIAVSHAETTMQYGIRE 187 +N AG FIRAWN DK GW IID +N+SLLFWAS T DPR++ I V+ AET +++ IR Sbjct: 123 FNLAGSFIRAWNLDKIGWSIIDSTMNLSLLFWASNETKDPRFRQIGVAQAETVLKHFIRP 182 Query: 188 DGSTKHILSFDAETGAYIENFGGQGYSPESCWSRGAAWGLYGFFNTYRHTGDERFLNTAK 247 DGS HILSFD ETGA++E GGQGY +S WSRG AW +YG N YR+TGD RFL+ AK Sbjct: 183 DGSVCHILSFDPETGAFLEPLGGQGYDKDSSWSRGQAWAIYGMANVYRYTGDRRFLDAAK 242 Query: 248 R 248 R Sbjct: 243 R 243 >B1D7I5.1 B1D7I5_9BACL Glycosyl hydrolase family 88 Length = 376 Score = 291 bits (745), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 137/240 (57%), Positives = 171/240 (71%), Gaps = 1/240 (0%) Query: 9 IMDKLDTKVTRMAEQIGGKCPHYAGADGKYDDMASDWWTTGFWPGLLWIMHDMTGKELYK 68 I L+ KV RM QIG K PH A ADG YDD DWWT+GFWPG+LW+M++ TG E YK Sbjct: 15 IWKNLEEKVGRMIVQIGEKSPHVAKADGIYDDQRIDWWTSGFWPGILWLMNEATGDEKYK 74 Query: 69 EAAWHWDATLEEWFVKPTVELHHDVGFQFLPTAVIKHTLTGDEDGLRRGIEAANFLAARY 128 AAW+WD LE ++P HDVGF FLPTAV K+ +TGD+D RRG+ AANF+A R+ Sbjct: 75 AAAWNWDERLEAQLLQPN-SFDHDVGFHFLPTAVAKYRITGDKDAQRRGLFAANFMAGRF 133 Query: 129 NPAGKFIRAWNEDKYGWVIIDCMLNISLLFWASKVTGDPRYKHIAVSHAETTMQYGIRED 188 NP G+F+RAWN D +GW IID +N+SLL+WAS +GD R+ HIA +HA+T + Y IRED Sbjct: 134 NPNGRFLRAWNGDMHGWSIIDTCMNLSLLYWASAESGDSRFAHIASAHADTVLNYFIRED 193 Query: 189 GSTKHILSFDAETGAYIENFGGQGYSPESCWSRGAAWGLYGFFNTYRHTGDERFLNTAKR 248 GS HI+ FD ETG + + GGQG +P S WSRGA+W LYG NTYR+TG +L A+R Sbjct: 194 GSVNHIVCFDPETGEFDQVRGGQGAAPNSGWSRGASWALYGMANTYRYTGKIEYLRAAQR 253 >B3KCR7.1 B3KCR7_9BACI Glycosyl hydrolase family 88 Length = 397 Score = 291 bits (744), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 143/245 (58%), Positives = 172/245 (70%), Gaps = 10/245 (4%) Query: 12 KLDTKVTRMAEQIGGKCPHYAGADGKYDDMASDWWTTGFWPGLLWIMHDMTGKELYKEAA 71 KL+ KVTRM Q+ GK PH A +G YD DWWT+GFWPG+LW+M+DMT + YKE A Sbjct: 18 KLEKKVTRMTGQLQGKVPH-ATKNGIYDSTRIDWWTSGFWPGMLWMMYDMTKQPQYKEWA 76 Query: 72 WHWDATLEEWFVKPTVELHHDVGFQFLPTAVIKHTLTGDEDGLRRGIEAANFLAARYNPA 131 W WD LEE + + HHDVGFQFLPTAVIK+ LTGD D RRG+ AA LA RYNP Sbjct: 77 WEWDIRLEEALIGDS-NFHHDVGFQFLPTAVIKYKLTGDPDARRRGLLAATLLAGRYNPV 135 Query: 132 GKFIRAWNEDK--------YGWVIIDCMLNISLLFWASKVTGDPRYKHIAVSHAETTMQY 183 G+FIRAWN+ K GW IID +N+SLLFWAS+ +GDPR+ HIA A T ++ Sbjct: 136 GRFIRAWNQVKPTSWNHGNEGWSIIDSTMNLSLLFWASEESGDPRFAHIAREQANTVAEH 195 Query: 184 GIREDGSTKHILSFDAETGAYIENFGGQGYSPESCWSRGAAWGLYGFFNTYRHTGDERFL 243 IR DGS +HI FD TG Y+E+ GGQG+ PES WSRGAAW L+G NTYR+TGD R+L Sbjct: 196 FIRPDGSVRHICRFDPLTGEYMESLGGQGFGPESAWSRGAAWALHGMANTYRYTGDIRYL 255 Query: 244 NTAKR 248 + AKR Sbjct: 256 DAAKR 260 >B1D7S3.1 B1D7S3_9BACL Glycosyl hydrolase family 88 Length = 384 Score = 237 bits (604), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 122/257 (47%), Positives = 162/257 (63%), Gaps = 17/257 (6%) Query: 7 QEIMDKLDTKVTRMAEQIGGKCPHYAGADGKYDDMASD-----------WWTTGFWPGLL 55 +++DK+ TK+ ++E+ K P Y +G +DD A D WWT GFW G++ Sbjct: 11 NQVIDKIRTKMDWVSEKSRNKIP-YTTINGTHDDRAVDNPSGYDTDGINWWTNGFWGGMM 69 Query: 56 WIMHDMTGKELYKEAAWHWDATLEEWFVKPTVELHHDVGFQFLPTAVIKHTLTGDEDGLR 115 W+MH TG E YKE A +A L++ F T LHHDVGF +LPT+V + +TG+ + + Sbjct: 70 WLMHHETGNEKYKEIAGMSEAKLDQCFHDYT-GLHHDVGFMWLPTSVANYRVTGNLESRK 128 Query: 116 RGIEAANFLAARYNPAGKFIRAWNE----DKYGWVIIDCMLNISLLFWASKVTGDPRYKH 171 R + AAN LA R+N AG FIRAWN+ D GW IIDCM NI LL+WA++ TGDPR+K Sbjct: 129 RAMHAANLLAGRFNLAGGFIRAWNDLDEGDTRGWAIIDCMFNIPLLYWATEETGDPRFKQ 188 Query: 172 IAVSHAETTMQYGIREDGSTKHILSFDAETGAYIENFGGQGYSPESCWSRGAAWGLYGFF 231 IA+ HA+TTM +R DGS HI+ FD G + +GGQGY S W+RG W +YGF Sbjct: 189 IAMKHADTTMSAFVRPDGSVNHIVEFDPFEGGVVRTYGGQGYEEGSSWTRGQTWAMYGFM 248 Query: 232 NTYRHTGDERFLNTAKR 248 +Y HTG E +L TAKR Sbjct: 249 MSYIHTGKEEYLATAKR 265 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,892,772,540 Number of extensions: 84766091 Number of successful extensions: 180836 Number of sequences better than 1.0e-05: 297 Number of HSP's gapped: 180220 Number of HSP's successfully gapped: 297 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)