BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0022.p (278 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BAB3.1 Q2BAB3_9BACI Multiple sugar-binding transport system pe... 219 2e-55 B3KDP8.1 B3KDP8_9BACI Binding-protein-dependent transport system... 212 4e-53 A6CLU7.1 A6CLU7_9BACI Multiple sugar-binding transport system pe... 210 1e-52 B1D7N8.1 B1D7N8_9BACL Binding-protein-dependent transport system... 205 3e-51 C4L2X2.1 C4L2X2_EXISA Binding-protein-dependent transport system... 205 3e-51 >Q2BAB3.1 Q2BAB3_9BACI Multiple sugar-binding transport system permease Length = 275 Score = 219 bits (557), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 108/250 (43%), Positives = 165/250 (66%) Query: 28 PIFFNLMSAFKSNAEIMKDAIAFPKGLYLDSFKYLLTETDFPRAIVNSLILTLVSIVAQV 87 P +F +++ KS AEI+ DA AFPK + ++ + +FPRA NSLI+T++S + V Sbjct: 26 PFYFVAVNSVKSFAEILLDAAAFPKEVLFANYSKVWEIINFPRAFWNSLIITVLSNIGLV 85 Query: 88 LIIPMAGYAIERRDTRWTKMVFLYFLAGMMIPFQAYMIPLFKELRVFGLYGSLAGPILVY 147 +I MA + + R R++K++F+ F++ M+IPFQ MIPL K + S+ G I++Y Sbjct: 86 IISSMAAWKMVRTPGRFSKILFILFVSAMVIPFQTVMIPLMKLGGALNMTNSIPGLIMMY 145 Query: 148 VAGAVGFGCLLYTSFVKGIPKEIEEAAEIDGCSRYGIFWKIVFPLLGPVTASMVVLNGLG 207 V LY FVK +P+EIEE+A IDGCS++G+FW+IVFPLL P+T ++V+LN L Sbjct: 146 FGFGVPLSLFLYHGFVKTVPREIEESALIDGCSQFGVFWRIVFPLLKPITVTVVILNTLW 205 Query: 208 IWNDFLMPMLVLPSGEPKTMVVEIYRYIGEFSSRWDMIFAGTAMSVVPVLIVFIALQKYF 267 IWNDFL+P+LVL E +T+ + + +++ +WDM A + + PV+I F+ LQK+ Sbjct: 206 IWNDFLLPLLVLQDAELRTIPLATSSFFAQYTKQWDMGLAALVLGITPVIIFFLFLQKHI 265 Query: 268 VKGIASGATK 277 +KGIASG+ K Sbjct: 266 IKGIASGSIK 275 >B3KDP8.1 B3KDP8_9BACI Binding-protein-dependent transport systems inner membrane component Length = 279 Score = 212 bits (539), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 100/255 (39%), Positives = 164/255 (64%) Query: 24 IFFFPIFFNLMSAFKSNAEIMKDAIAFPKGLYLDSFKYLLTETDFPRAIVNSLILTLVSI 83 IF P +F L+++ KS +IM DA +P + D++ T FP A NS+I+T++S Sbjct: 25 IFLAPFYFLLVNSVKSLGDIMVDAANWPTAFHFDNYSKAWEMTRFPEAFTNSIIITVISN 84 Query: 84 VAQVLIIPMAGYAIERRDTRWTKMVFLYFLAGMMIPFQAYMIPLFKELRVFGLYGSLAGP 143 + L+ MA Y + R +TR+ ++VF+ F++ M+IPFQ+ MIPL + + G+ S+ G Sbjct: 85 LIIALLSAMAAYRMVRSNTRFNRIVFMLFVSAMVIPFQSIMIPLLQVINFLGVNNSIVGL 144 Query: 144 ILVYVAGAVGFGCLLYTSFVKGIPKEIEEAAEIDGCSRYGIFWKIVFPLLGPVTASMVVL 203 IL Y+ + L+ FVKGIP EIEEAA +DG S + +F +IV P+L P+ ++++L Sbjct: 145 ILSYLGLGIPLSVFLFHGFVKGIPLEIEEAATVDGASPFRVFARIVMPMLKPMMVTVIIL 204 Query: 204 NGLGIWNDFLMPMLVLPSGEPKTMVVEIYRYIGEFSSRWDMIFAGTAMSVVPVLIVFIAL 263 N L +WND+L+P L+L S E +T+ + + + G++S +WDM + + P++I F++L Sbjct: 205 NCLWVWNDYLLPSLILQSPELRTIPLATFAFFGQYSKQWDMALPALVLGITPIIIFFLSL 264 Query: 264 QKYFVKGIASGATKG 278 QKY V+G+A+G+ KG Sbjct: 265 QKYIVEGVAAGSVKG 279 >A6CLU7.1 A6CLU7_9BACI Multiple sugar-binding transport system permease Length = 275 Score = 210 bits (534), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 104/254 (40%), Positives = 163/254 (64%) Query: 24 IFFFPIFFNLMSAFKSNAEIMKDAIAFPKGLYLDSFKYLLTETDFPRAIVNSLILTLVSI 83 IF P +F L+++ K A I+ DA A+PK ++ + +FPRA NSL++T+ S Sbjct: 22 IFLIPFYFVLVNSVKPFAAILIDAAAWPKEFLFSNYAKVWEIINFPRAFWNSLVITVFSN 81 Query: 84 VAQVLIIPMAGYAIERRDTRWTKMVFLYFLAGMMIPFQAYMIPLFKELRVFGLYGSLAGP 143 + V+I MA + + R +++K++F+ F++ M+IPFQ MIPL K L S+ G Sbjct: 82 IGLVIISSMAAWKMVRTPGKFSKILFIIFVSAMVIPFQTVMIPLMKLGGALNLTNSIPGL 141 Query: 144 ILVYVAGAVGFGCLLYTSFVKGIPKEIEEAAEIDGCSRYGIFWKIVFPLLGPVTASMVVL 203 I++Y V L+ FVK +P EIEE+A IDGCS++G+FW+IVFPLL P+T ++++L Sbjct: 142 IIMYFGFGVPLSLFLFHGFVKTVPMEIEESAMIDGCSQFGVFWRIVFPLLKPITVTVIIL 201 Query: 204 NGLGIWNDFLMPMLVLPSGEPKTMVVEIYRYIGEFSSRWDMIFAGTAMSVVPVLIVFIAL 263 N L IWND+L+P+LVL E +T+ + + +++ +WDM A + + PV+I F+ L Sbjct: 202 NTLWIWNDYLLPLLVLQDAELRTIPLAASSFFAQYTKQWDMGLAALVLGITPVIIFFLFL 261 Query: 264 QKYFVKGIASGATK 277 QK+ +KGIASG+ K Sbjct: 262 QKHIIKGIASGSIK 275 >B1D7N8.1 B1D7N8_9BACL Binding-protein-dependent transport systems inner membrane component Length = 276 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 94/252 (37%), Positives = 165/252 (65%), Gaps = 1/252 (0%) Query: 28 PIFFNLMSAFKSNAEIMKDAIAFPKGLYLDSFKYLLTETDFPRAIVNSLILTLVSIVAQV 87 P +F L+++ K EIM DA + P ++ + FP+A++NS+++T +S+ V Sbjct: 25 PFYFVLVNSVKGLGEIMVDAASLPDKYLFTNYSKVWDIIKFPKALLNSVLITGISVAGIV 84 Query: 88 LIIPMAGYAIERRDTRWTKMVFLYFLAGMMIPFQAYMIPLFKELRVFGLYGSLAGPILVY 147 +I M GY + R+ T + K++F F++ M+IPFQ+ M+ L + L ++ G ++ Y Sbjct: 85 IISAMTGYRLVRKPTLFNKILFTAFISAMIIPFQSVMLQLVRVTSFLELRANMFGIVVCY 144 Query: 148 VAGAVGFGCLLYTSFVKGIPKEIEEAAEIDGCSRYGIFWKIVFPLLGPVTASMVVLNGLG 207 + + L+ F+K +P EIEEAA +DGCS YG+FW+IVFPLL P+ ++++LNGL Sbjct: 145 LGFGLSLSVFLFHGFIKSVPLEIEEAAVVDGCSPYGVFWRIVFPLLRPIIVTVIILNGLW 204 Query: 208 IWNDFLMPMLVLPSGEPKTMV-VEIYRYIGEFSSRWDMIFAGTAMSVVPVLIVFIALQKY 266 IWND+L+P+L++ S + T + + I R+ G+++ +WD+ AG M ++P++++F+ LQK+ Sbjct: 205 IWNDYLLPVLIIGSNKDLTTIPLAITRFFGQYTKQWDLALAGLTMGIMPIIVMFLMLQKH 264 Query: 267 FVKGIASGATKG 278 ++GI+SG+ KG Sbjct: 265 IIEGISSGSLKG 276 >C4L2X2.1 C4L2X2_EXISA Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 100/254 (39%), Positives = 157/254 (61%) Query: 24 IFFFPIFFNLMSAFKSNAEIMKDAIAFPKGLYLDSFKYLLTETDFPRAIVNSLILTLVSI 83 ++ P ++ + ++FKS AEI + A P ++ ++ R +NSL++T+ + Sbjct: 47 LYLVPFYYVVANSFKSMAEIFTNTSALPNEWLTSNYTEAWEAMNYGRVFLNSLLITVGAN 106 Query: 84 VAQVLIIPMAGYAIERRDTRWTKMVFLYFLAGMMIPFQAYMIPLFKELRVFGLYGSLAGP 143 V+ MA + + R + M+F F+A M+IPFQ+ MIPL K + GL S G Sbjct: 107 AVIVIFTSMAAWKLVRTKHWISTMIFFLFVAAMVIPFQSIMIPLVKVSSILGLLNSHWGL 166 Query: 144 ILVYVAGAVGFGCLLYTSFVKGIPKEIEEAAEIDGCSRYGIFWKIVFPLLGPVTASMVVL 203 I++Y+ G LY F+K IP+EIEEAA IDGCS +G+FW+IVFPLL P+T ++V+L Sbjct: 167 IVMYLGFGSGISVFLYHGFMKSIPEEIEEAAIIDGCSTWGVFWRIVFPLLKPITVTIVIL 226 Query: 204 NGLGIWNDFLMPMLVLPSGEPKTMVVEIYRYIGEFSSRWDMIFAGTAMSVVPVLIVFIAL 263 N L IWNDFL+P LVL E +T+ + + + G+++ +WD+ AG + +VP+L+ F + Sbjct: 227 NSLWIWNDFLLPSLVLRDVELRTIPLATFYFFGQYTKQWDLALAGLVLGIVPLLVFFFGM 286 Query: 264 QKYFVKGIASGATK 277 QK+ +KGI SG+ K Sbjct: 287 QKHIIKGITSGSIK 300 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.145 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,494,089,427 Number of extensions: 60281002 Number of successful extensions: 209102 Number of sequences better than 1.0e-05: 6347 Number of HSP's gapped: 201925 Number of HSP's successfully gapped: 6347 Length of query: 278 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 144 Effective length of database: 1,798,280,193 Effective search space: 258952347792 Effective search space used: 258952347792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)