BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0008.p (238 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6TPG6.1 A6TPG6_ALKMQ Bacitracin ABC transporter, permease protein 307 5e-82 Q8TSE6.1 Q8TSE6_METAC Bacitracin ABC transporter, permease protein 129 2e-28 A3CPP4.1 A3CPP4_STRSV Bacitracin ABC transporter, permease prote... 107 8e-22 B3KFD7.1 B3KFD7_9BACI Bacitracin ABC transporter, permease protein 89 3e-16 Q97DF8.1 Q97DF8_CLOAB Predicted membrane protein, possible permease 87 2e-15 >A6TPG6.1 A6TPG6_ALKMQ Bacitracin ABC transporter, permease protein Length = 238 Score = 307 bits (786), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 153/238 (64%), Positives = 181/238 (76%) Query: 1 MNILSVLHCEIRKIIRSNVFWIMFLVLGFGPVMMGVGNVLSSDAGGVQWEAYLTGLLDTL 60 MNI+S+LHCEIRKIIRSNVFW++FLV FGP+MMGVG +LSS+ GG+ W+ YLT L + L Sbjct: 1 MNIISMLHCEIRKIIRSNVFWLVFLVFAFGPIMMGVGIILSSNTGGINWQMYLTELHNNL 60 Query: 61 APLGLIGYTFVAAWVFGREFSDRTIKDLLAKPISRAKIVLSKFLVILAWCLLLSIYMFXX 120 A LG+IGYTF+AAWVFGREF+D+TIKDLLAKPI R++IV+SK LVILAW +LLS+YMF Sbjct: 61 AALGVIGYTFIAAWVFGREFTDKTIKDLLAKPIPRSQIVMSKLLVILAWNVLLSVYMFAI 120 Query: 121 XXXXXXXXXXXXXXXXXIWSLFIKFLITSLLYIFVTAPSILLANVTKGYLAPXXXXXXXX 180 IW+ F +F ITSLL+I T P LLANV+KGYLAP Sbjct: 121 GLAVGGVFGLTGWSTYLIWNSFFRFTITSLLFIVATTPGALLANVSKGYLAPLGLILVIV 180 Query: 181 XXSNVLASFGFAPYFPWTIPSVFQSTGSLQLSSIVILAYTGIIGIAGTFAWWRFAEQQ 238 S+V+A+ GFAPYFPWTIPSV+ STGSL LSSI+ILA TGI GI GTF WWRFAEQQ Sbjct: 181 IFSSVMATLGFAPYFPWTIPSVYLSTGSLNLSSIIILASTGIAGIVGTFVWWRFAEQQ 238 >Q8TSE6.1 Q8TSE6_METAC Bacitracin ABC transporter, permease protein Length = 258 Score = 129 bits (325), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 82/255 (32%), Positives = 124/255 (48%), Gaps = 19/255 (7%) Query: 2 NILSVLHCEIRKIIRSNVFWIMFLVLGF-------------GPVMMGVGNVLSSDA---G 45 I S + E K+ RS W F V+ PV+ +LS A G Sbjct: 3 GIESAIWAEFLKMRRSTALWATFAVVSIMILMMGLMMFVLKNPVLAKDLGLLSVKAQLFG 62 Query: 46 GVQWEAYLTGLLDTLAPLGLIGYTFVAAWVFGREFSDRTIKDLLAKPISRAKIVLSKFLV 105 W ++ T +++ + ++ + FV++WVFGRE+SDRT KDLLA P+SR IV+SKF+V Sbjct: 63 AADWPSFFTIFSESMPGMEMVIFGFVSSWVFGREYSDRTAKDLLALPVSRTSIVISKFIV 122 Query: 106 ILAWCLLLSIYMFXXXXXXXXXXXXXXXXXXXIWSLFIKFLITSLLYIFVTAPSILLANV 165 IL WC++L ++ F + FI + L +F+ L+A+ Sbjct: 123 ILIWCVVLYLFAFALAITVGNVIQLSGWSFGHAFDRFIVLFEVAFLAVFLDTTVALIASY 182 Query: 166 TKGYLAPXXXXXXXXXXSNVLASFGFAPYFPWTIPSVFQSTG---SLQLSSIVILAYTGI 222 T+GYLA N + G+ Y+PW I ++ + G L +S +I+ TG Sbjct: 183 TRGYLAAIGFIIFAMVIVNFALTLGYGQYYPWAIAMLYATEGMEEQLSATSWIIVFITGS 242 Query: 223 IGIAGTFAWWRFAEQ 237 IG GT AWWR+A+Q Sbjct: 243 IGFFGTLAWWRYADQ 257 >A3CPP4.1 A3CPP4_STRSV Bacitracin ABC transporter, permease protein, putative Length = 247 Score = 107 bits (267), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 64/194 (32%), Positives = 96/194 (49%), Gaps = 3/194 (1%) Query: 48 QWEAYLTGLLDTLAPLGLIGYTFVAAWVFGREFSDRTIKDLLAKPISRAKIVLSKFLVIL 107 W +YL + ++ GL+ + F A+WVFGRE+SD+T+ DLLA P+ R IVLSKF+VI Sbjct: 54 DWSSYLMTVSQVISVGGLLIFGFTASWVFGREYSDKTMIDLLALPMKRDTIVLSKFIVIA 113 Query: 108 AWCLLLSIYMFXXXXXXXXXXXXXXXXXXXIWSLFIKFLITSLLYIFVTAPSILLANVTK 167 W +LSI+ I+ + F + L I ++ P A + + Sbjct: 114 LWSYILSIFALLLGLLAGNLIGLEGGSLTVIFKGILTFAFCTTLTIVLSTPVAFFACLGR 173 Query: 168 GYLAPXXXXXXXXXXSNVLASFGFAPYFPWTIPSV---FQSTGSLQLSSIVILAYTGIIG 224 GYL+P S + ++ F Y PW IP++ + L SIV L I+G Sbjct: 174 GYLSPLAFIIFTIIFSQLGSALNFGKYIPWAIPALASGIEGKALLNFGSIVSLFGVSILG 233 Query: 225 IAGTFAWWRFAEQQ 238 + T AWWR+A+ Sbjct: 234 LIATVAWWRYADYD 247 >B3KFD7.1 B3KFD7_9BACI Bacitracin ABC transporter, permease protein Length = 236 Score = 89.0 bits (219), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 71/242 (29%), Positives = 113/242 (46%), Gaps = 15/242 (6%) Query: 3 ILSVLHCEIRKIIRSNVFWIMFLVLGFGPVMMGVGNVLSSDAGGVQWEAYLTGLLDTLAP 62 +L+ E+ K+ RS V W L + F V++ G W ++ A Sbjct: 4 LLTACSVEVYKLRRSFVLWGTLLFILF---------VITLFLGQPDWPSFFERTAFLYAS 54 Query: 63 L-GLIGYTFVAAWVFGREFSDRTIKDLLAKPISRAKIVLSKFLVILAWCLLLSIYMFXXX 121 + GL+G+ V +W FGRE+SDRT+KDLL PISR KI ++K++ I+ WCL++ + F Sbjct: 55 VFGLLGFGLVTSWTFGREYSDRTLKDLLVLPISRGKIAIAKYVAIIFWCLVMMLISFAYA 114 Query: 122 XXXXXXXXXXXXXXXXIWSLFIKFLITSLLYIFVTAPSILLANVTKGYLAPXXXXXXXXX 181 + LI L++ ++AP LLA +++GYL P Sbjct: 115 LILGFAIGLPGFSFAIVQHYLFLILIIGALHLLLSAPLALLACISRGYLVPIGFAFTTLM 174 Query: 182 XSNVLASFGFAPYFPWTIPSV-FQSTG----SLQLSSIVILAYTGIIGIAGTFAWWRFAE 236 + V Y PW++P++ Q G L +S ++ G+ G GTF W + E Sbjct: 175 LALVFGPTAIGKYLPWSVPALHLQENGIASFPLAETSYFLVFLIGLAGFIGTFKWLQNRE 234 Query: 237 QQ 238 Q+ Sbjct: 235 QR 236 >Q97DF8.1 Q97DF8_CLOAB Predicted membrane protein, possible permease Length = 217 Score = 86.7 bits (213), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 55/192 (28%), Positives = 95/192 (49%), Gaps = 2/192 (1%) Query: 49 WEAYLTG-LLDTLAPLGLIGYTFVAAWVFGREFSDRTIKDLLAKPISRAKIVLSKFLVIL 107 WEA+L G L +GLIG++ ++ WVFGRE+ D+T KDLL+ PI R +V +KFL + Sbjct: 26 WEAFLKGTFFMYLTVIGLIGFSILSTWVFGREYQDQTYKDLLSLPIPRTTLVTAKFLALE 85 Query: 108 AWCLLLSIYMFXXXXXXXXXXXXXXXXXXXIWSLFIKFLITSLLYIFVTAPSILLANVTK 167 + +++ ++ + ++ +L I ++ L+A++ + Sbjct: 86 LSFVGITVIAIMATFSLGYCFNLADFNVSLAGNIVKRLVMATLYNIGLSFLFPLIASMVR 145 Query: 168 GYLAPXXXXXXXXXXSNVLASFGFAPYFPWTIPSVFQSTGSL-QLSSIVILAYTGIIGIA 226 G LAP + V S PY PW+IP ++ + L L S + ++ IG+ Sbjct: 146 GVLAPVSISFVALIMAVVFGSQSVGPYIPWSIPGIYLNNPQLISLVSRMTISIIVFIGVY 205 Query: 227 GTFAWWRFAEQQ 238 GT WW + +Q+ Sbjct: 206 GTILWWNYVDQK 217 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.143 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,197,019,609 Number of extensions: 44016305 Number of successful extensions: 151890 Number of sequences better than 1.0e-05: 87 Number of HSP's gapped: 151826 Number of HSP's successfully gapped: 87 Length of query: 238 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 106 Effective length of database: 1,817,122,223 Effective search space: 192614955638 Effective search space used: 192614955638 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 129 (54.3 bits)