BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0009.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LVL6.1 A6LVL6_CLOB8 Enoyl-CoA hydratase/isomerase 478 e-133 A6LVL5.1 A6LVL5_CLOB8 Enoyl-CoA hydratase/isomerase 416 e-114 C4E2T5.1 C4E2T5_STRRS Enoyl-CoA hydratase/carnithine racemase 291 5e-77 Q11L40.1 Q11L40_MESSB Enoyl-CoA hydratase/isomerase 160 8e-38 A0QWH9.1 A0QWH9_MYCS2 6-oxocamphor hydrolase 154 1e-35 >A6LVL6.1 A6LVL6_CLOB8 Enoyl-CoA hydratase/isomerase Length = 278 Score = 478 bits (1229), Expect = e-133, Method: Compositional matrix adjust. Identities = 218/273 (79%), Positives = 244/273 (89%) Query: 1 MIGPTRFEEYSEKYKDLFLMTRHDGILEVRMHTNGGPLQFDWPVQAAYGHVWSDIGRDPE 60 M+GPTRFEEYSEKYKD F+MTR +GI+EVR HTNGGP QFDWP Q AYG+VWSDIGRDPE Sbjct: 6 MVGPTRFEEYSEKYKDFFIMTRRNGIIEVRFHTNGGPFQFDWPGQTAYGNVWSDIGRDPE 65 Query: 61 NEVMILTGTGELWQIGNPEVWTTKFMDWPNRRKLEMYHESLRMIENMVHCLDIPTIGAIN 120 NEVMILTGTG+LWQIGNPEVW TKFMDWP RKLEMYHESLRMIENM++C+DIPTIGAIN Sbjct: 66 NEVMILTGTGDLWQIGNPEVWKTKFMDWPRNRKLEMYHESLRMIENMIYCIDIPTIGAIN 125 Query: 121 GTGAHWQLGTLCDITICAEDTAFFDAHYLGGVPPGDGIVLALQKILGSKKAAYYAYTGLN 180 GTG+HWQLGT CDITICAE T FFDAHYLGG+PPGDGIVL LQ +LG K+AA+YAYTG+N Sbjct: 126 GTGSHWQLGTFCDITICAEGTEFFDAHYLGGIPPGDGIVLTLQNLLGIKRAAFYAYTGMN 185 Query: 181 ITAQEALDLGLISEVLPREQLLPRAWGLAEMIMQAPRSTRHLTHSIVSHPWKQAFAENQG 240 I AQEAL+LG++SEVLPRE+LLPRAW LAEMIMQAPRS RHLTHSI+S PWK+A +QG Sbjct: 186 INAQEALNLGMVSEVLPREKLLPRAWELAEMIMQAPRSARHLTHSILSRPWKKALVSDQG 245 Query: 241 LQLTHQLVDMAIDEEGIHERLMKLKERFQRNGR 273 QLT QL DM+IDEEG+ ERL+K+K RFQ N R Sbjct: 246 FQLTRQLYDMSIDEEGVLERLVKIKGRFQGNER 278 >A6LVL5.1 A6LVL5_CLOB8 Enoyl-CoA hydratase/isomerase Length = 276 Score = 416 bits (1070), Expect = e-114, Method: Compositional matrix adjust. Identities = 185/268 (69%), Positives = 222/268 (82%) Query: 1 MIGPTRFEEYSEKYKDLFLMTRHDGILEVRMHTNGGPLQFDWPVQAAYGHVWSDIGRDPE 60 MIGPT+FEEYSEKYK+ MTR DGI+EVR+HTNGGP + +W A+ H WSDIGRDPE Sbjct: 6 MIGPTKFEEYSEKYKEFLSMTRRDGIIEVRLHTNGGPYEHNWEAHTAWSHAWSDIGRDPE 65 Query: 61 NEVMILTGTGELWQIGNPEVWTTKFMDWPNRRKLEMYHESLRMIENMVHCLDIPTIGAIN 120 NEVMI++GTG+ W +GNP+VW TKFMDWP ++KLE YHESLR++ENM+ C+DIPTIGAIN Sbjct: 66 NEVMIISGTGDKWVVGNPDVWNTKFMDWPKQKKLEQYHESLRLLENMIFCIDIPTIGAIN 125 Query: 121 GTGAHWQLGTLCDITICAEDTAFFDAHYLGGVPPGDGIVLALQKILGSKKAAYYAYTGLN 180 G G H ++ +LCDITIC ED FFD H++GG PPGDG++L LQ ++G KKAAYYAYTG N Sbjct: 126 GPGTHCEIASLCDITICTEDADFFDPHFIGGTPPGDGMILTLQNMIGFKKAAYYAYTGKN 185 Query: 181 ITAQEALDLGLISEVLPREQLLPRAWGLAEMIMQAPRSTRHLTHSIVSHPWKQAFAENQG 240 I Q AL+LG++SEVLPRE+LLPRAW LAEMIMQAPRS RHLTHSI+S PWK+A +QG Sbjct: 186 INGQTALELGIVSEVLPREKLLPRAWELAEMIMQAPRSARHLTHSILSRPWKKALVSDQG 245 Query: 241 LQLTHQLVDMAIDEEGIHERLMKLKERF 268 QLTHQ+ DMAIDEEG+ ERL K+K R Sbjct: 246 FQLTHQMYDMAIDEEGVLERLTKMKGRL 273 >C4E2T5.1 C4E2T5_STRRS Enoyl-CoA hydratase/carnithine racemase Length = 307 Score = 291 bits (744), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 142/269 (52%), Positives = 191/269 (71%), Gaps = 1/269 (0%) Query: 2 IGPTRFEEYSEKYKDLFLMTRHDGILEVRMHTNGGPLQFDWPVQAAYGHVWSDIGRDPEN 61 + PT FEEYS KY++ F+MTR +GI+E+RMHT+GGP + W A+ W+++G DPEN Sbjct: 29 VKPTPFEEYSRKYEEFFVMTRREGIIELRMHTDGGPFRHSWIGHNAWHQAWAEVGNDPEN 88 Query: 62 EVMILTGTGELWQIGNP-EVWTTKFMDWPNRRKLEMYHESLRMIENMVHCLDIPTIGAIN 120 EV+I+TGTG+ W G+P E+W T F W +L+MY + ++++EN V LDIPTI A+N Sbjct: 89 EVLIITGTGDRWHTGDPAELWHTPFPQWSTDSQLKMYGDMVKLLENFVSALDIPTIAAVN 148 Query: 121 GTGAHWQLGTLCDITICAEDTAFFDAHYLGGVPPGDGIVLALQKILGSKKAAYYAYTGLN 180 G G H + T CDIT+C ED FFD H+LGG PGDG+ L LQ +G+K+A+YY YTG Sbjct: 149 GPGTHCEFATACDITLCVEDADFFDPHFLGGGVPGDGMGLTLQHTIGTKRASYYMYTGEP 208 Query: 181 ITAQEALDLGLISEVLPREQLLPRAWGLAEMIMQAPRSTRHLTHSIVSHPWKQAFAENQG 240 I AL LGL++EVLPRE+LLPRAW +AEM+M PR++R LTH+I S PWK+AF ++ G Sbjct: 209 IDGPTALRLGLVNEVLPRERLLPRAWEIAEMMMARPRASRLLTHAIASRPWKRAFLDDLG 268 Query: 241 LQLTHQLVDMAIDEEGIHERLMKLKERFQ 269 HQL MAI E G +RL ++ ERF+ Sbjct: 269 FHAAHQLWGMAIGEAGQLDRLRQMTERFR 297 >Q11L40.1 Q11L40_MESSB Enoyl-CoA hydratase/isomerase Length = 254 Score = 160 bits (406), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 91/237 (38%), Positives = 140/237 (59%), Gaps = 15/237 (6%) Query: 5 TRFEEYSEKYKDLFLMTRHDGILEVRMHTNGGPLQFDWPVQAAYGHVWSDIGRDPENEVM 64 + F +Y+EKY + M R DGILE+R HT+GGP Q+ A + D+GRD +NEV+ Sbjct: 2 STFADYAEKYS-MIKMRREDGILEMRFHTDGGPFQWSLIPHAELEQAFLDVGRDYDNEVV 60 Query: 65 ILTGTGELW------QIGNPEVWTTKFMDWPNRRKLEMYHESLRMIENMVHCLDIPTIGA 118 ILTGTG+ + + G+P PNR +Y E+ ++ N+++ +++P I A Sbjct: 61 ILTGTGDEYCGPLVKEGGHPNRANAT----PNRYD-RIYWEAKHLLNNLLN-IEVPVISA 114 Query: 119 INGTGA-HWQLGTLCDITICAEDTAFFD-AHYLGGVPPGDGIVLALQKILGSKKAAYYAY 176 ING H ++ L DI + ++ T F D AHY GG+ PGDG+ + ++G+ +A YY Sbjct: 115 INGPAVRHAEIPLLSDIVLASDTTYFMDSAHYQGGMVPGDGVHIIFPLLMGANRARYYLL 174 Query: 177 TGLNITAQEALDLGLISEVLPREQLLPRAWGLAEMIMQAPRSTRHLTHSIVSHPWKQ 233 TG I A EA +GL++EVLP ++LPRAW LA +M+ PR R + +++ K+ Sbjct: 175 TGQKIPADEAKAVGLVNEVLPANEVLPRAWELARQLMKQPRLVRRYSRVVLTQDLKE 231 >A0QWH9.1 A0QWH9_MYCS2 6-oxocamphor hydrolase Length = 271 Score = 154 bits (388), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 87/238 (36%), Positives = 141/238 (59%), Gaps = 15/238 (6%) Query: 4 PTRFEEYSEKYKDLFLMTRHDGILEVRMHTNGGPLQFDWPVQAAYGHVWSDIGRDPENEV 63 PT F+ Y ++Y+ + M R DG+LE+ HTNGGPLQ+ + + +IGRD EN+V Sbjct: 21 PTTFDSYKDRYETI-RMERTDGVLELHFHTNGGPLQWSLLAHNEFEDAFLEIGRDRENDV 79 Query: 64 MILTGTGELWQIGNPEVWTTKFMDWPNRRKL--EMYH----ESLRMIENMVHCLDIPTIG 117 +I+TGTGE + P + + PNRR + E Y E +++ N++ ++ P I Sbjct: 80 VIMTGTGE--EFSGPAIAPGQH---PNRRTMTPETYDPIQWEGKQLLPNLL-SIEAPVIA 133 Query: 118 AINGTGA-HWQLGTLCDITICAEDTAFFD-AHYLGGVPPGDGIVLALQKILGSKKAAYYA 175 AING H ++ + DI + EDT F D AH+ GG+ PGDG+ + + ++G +A Y+ Sbjct: 134 AINGPAVRHAEIPLVSDIVLATEDTYFQDTAHFPGGMVPGDGMHIVMPLLMGPTRARYFL 193 Query: 176 YTGLNITAQEALDLGLISEVLPREQLLPRAWGLAEMIMQAPRSTRHLTHSIVSHPWKQ 233 TG ++ EA ++GL++EV+PR+ LL RA LA +M+ PR R + ++++ +Q Sbjct: 194 LTGQQLSVAEAREIGLVNEVVPRDDLLSRARELAAQLMRQPRLVRRYSRTVLTEDLRQ 251 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,770,690,481 Number of extensions: 76787782 Number of successful extensions: 158838 Number of sequences better than 1.0e-05: 1568 Number of HSP's gapped: 159673 Number of HSP's successfully gapped: 1568 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)