BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0008.p (279 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LVL5.1 A6LVL5_CLOB8 Enoyl-CoA hydratase/isomerase 514 e-144 A6LVL6.1 A6LVL6_CLOB8 Enoyl-CoA hydratase/isomerase 421 e-116 C4E2T5.1 C4E2T5_STRRS Enoyl-CoA hydratase/carnithine racemase 326 1e-87 Q11L40.1 Q11L40_MESSB Enoyl-CoA hydratase/isomerase 157 1e-36 B5WG86.1 B5WG86_9BURK Enoyl-CoA hydratase/isomerase 155 5e-36 >A6LVL5.1 A6LVL5_CLOB8 Enoyl-CoA hydratase/isomerase Length = 276 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 238/276 (86%), Positives = 257/276 (93%) Query: 1 MTERRMIGPTXXXXXXXXXXXXLLMTRRDGIIEVRLHTGGGPYKHNWEAHTAWSHAWSDI 60 M+E RMIGPT L MTRRDGIIEVRLHT GGPY+HNWEAHTAWSHAWSDI Sbjct: 1 MSENRMIGPTKFEEYSEKYKEFLSMTRRDGIIEVRLHTNGGPYEHNWEAHTAWSHAWSDI 60 Query: 61 GRDPENEVMIISGTGDKWVIGDPEVWNTKFMDWPKQKKLEQYHESLRLLENLIFCIDIPT 120 GRDPENEVMIISGTGDKWV+G+P+VWNTKFMDWPKQKKLEQYHESLRLLEN+IFCIDIPT Sbjct: 61 GRDPENEVMIISGTGDKWVVGNPDVWNTKFMDWPKQKKLEQYHESLRLLENMIFCIDIPT 120 Query: 121 IGAVNGPGTHCELATLCDITICTEDADFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYA 180 IGA+NGPGTHCE+A+LCDITICTEDADFFDPH++GGTPPGDGM+LTLQNMIGFKKAAYYA Sbjct: 121 IGAINGPGTHCEIASLCDITICTEDADFFDPHFIGGTPPGDGMILTLQNMIGFKKAAYYA 180 Query: 181 YTGKNINGQTALDLGIVSEVLPREQLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQAL 240 YTGKNINGQTAL+LGIVSEVLPRE+LLPRAWELAEMIMQAPRS RHL+HSI+SRPWK+AL Sbjct: 181 YTGKNINGQTALELGIVSEVLPREKLLPRAWELAEMIMQAPRSARHLTHSILSRPWKKAL 240 Query: 241 VADQGFQLAHQMYDMAIDEEGALERLKKMQGRLMGK 276 V+DQGFQL HQMYDMAIDEEG LERL KM+GRLMGK Sbjct: 241 VSDQGFQLTHQMYDMAIDEEGVLERLTKMKGRLMGK 276 >A6LVL6.1 A6LVL6_CLOB8 Enoyl-CoA hydratase/isomerase Length = 278 Score = 421 bits (1081), Expect = e-116, Method: Compositional matrix adjust. Identities = 188/277 (67%), Positives = 229/277 (82%) Query: 1 MTERRMIGPTXXXXXXXXXXXXLLMTRRDGIIEVRLHTGGGPYKHNWEAHTAWSHAWSDI 60 M++R M+GPT +MTRR+GIIEVR HT GGP++ +W TA+ + WSDI Sbjct: 1 MSDRCMVGPTRFEEYSEKYKDFFIMTRRNGIIEVRFHTNGGPFQFDWPGQTAYGNVWSDI 60 Query: 61 GRDPENEVMIISGTGDKWVIGDPEVWNTKFMDWPKQKKLEQYHESLRLLENLIFCIDIPT 120 GRDPENEVMI++GTGD W IG+PEVW TKFMDWP+ +KLE YHESLR++EN+I+CIDIPT Sbjct: 61 GRDPENEVMILTGTGDLWQIGNPEVWKTKFMDWPRNRKLEMYHESLRMIENMIYCIDIPT 120 Query: 121 IGAVNGPGTHCELATLCDITICTEDADFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYA 180 IGA+NG G+H +L T CDITIC E +FFD HYLGG PPGDG++LTLQN++G K+AA+YA Sbjct: 121 IGAINGTGSHWQLGTFCDITICAEGTEFFDAHYLGGIPPGDGIVLTLQNLLGIKRAAFYA 180 Query: 181 YTGKNINGQTALDLGIVSEVLPREQLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQAL 240 YTG NIN Q AL+LG+VSEVLPRE+LLPRAWELAEMIMQAPRS RHL+HSI+SRPWK+AL Sbjct: 181 YTGMNINAQEALNLGMVSEVLPREKLLPRAWELAEMIMQAPRSARHLTHSILSRPWKKAL 240 Query: 241 VADQGFQLAHQMYDMAIDEEGALERLKKMQGRLMGKD 277 V+DQGFQL Q+YDM+IDEEG LERL K++GR G + Sbjct: 241 VSDQGFQLTRQLYDMSIDEEGVLERLVKIKGRFQGNE 277 >C4E2T5.1 C4E2T5_STRRS Enoyl-CoA hydratase/carnithine racemase Length = 307 Score = 326 bits (836), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 157/270 (58%), Positives = 203/270 (75%), Gaps = 1/270 (0%) Query: 7 IGPTXXXXXXXXXXXXLLMTRRDGIIEVRLHTGGGPYKHNWEAHTAWSHAWSDIGRDPEN 66 + PT +MTRR+GIIE+R+HT GGP++H+W H AW AW+++G DPEN Sbjct: 29 VKPTPFEEYSRKYEEFFVMTRREGIIELRMHTDGGPFRHSWIGHNAWHQAWAEVGNDPEN 88 Query: 67 EVMIISGTGDKWVIGDP-EVWNTKFMDWPKQKKLEQYHESLRLLENLIFCIDIPTIGAVN 125 EV+II+GTGD+W GDP E+W+T F W +L+ Y + ++LLEN + +DIPTI AVN Sbjct: 89 EVLIITGTGDRWHTGDPAELWHTPFPQWSTDSQLKMYGDMVKLLENFVSALDIPTIAAVN 148 Query: 126 GPGTHCELATLCDITICTEDADFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKN 185 GPGTHCE AT CDIT+C EDADFFDPH+LGG PGDGM LTLQ+ IG K+A+YY YTG+ Sbjct: 149 GPGTHCEFATACDITLCVEDADFFDPHFLGGGVPGDGMGLTLQHTIGTKRASYYMYTGEP 208 Query: 186 INGQTALDLGIVSEVLPREQLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQALVADQG 245 I+G TAL LG+V+EVLPRE+LLPRAWE+AEM+M PR++R L+H+I SRPWK+A + D G Sbjct: 209 IDGPTALRLGLVNEVLPRERLLPRAWEIAEMMMARPRASRLLTHAIASRPWKRAFLDDLG 268 Query: 246 FQLAHQMYDMAIDEEGALERLKKMQGRLMG 275 F AHQ++ MAI E G L+RL++M R G Sbjct: 269 FHAAHQLWGMAIGEAGQLDRLRQMTERFRG 298 >Q11L40.1 Q11L40_MESSB Enoyl-CoA hydratase/isomerase Length = 254 Score = 157 bits (396), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 88/233 (37%), Positives = 132/233 (56%), Gaps = 14/233 (6%) Query: 25 MTRRDGIIEVRLHTGGGPYKHNWEAHTAWSHAWSDIGRDPENEVMIISGTGDKWVI---- 80 M R DGI+E+R HT GGP++ + H A+ D+GRD +NEV+I++GTGD++ Sbjct: 16 MRREDGILEMRFHTDGGPFQWSLIPHAELEQAFLDVGRDYDNEVVILTGTGDEYCGPLVK 75 Query: 81 --GDPEVWNTKFMDWPKQKKLEQYHESLRLLENLIFCIDIPTIGAVNGPGT-HCELATLC 137 G P N + + Y E+ LL NL+ I++P I A+NGP H E+ L Sbjct: 76 EGGHPNRANATPNRYDRI-----YWEAKHLLNNLL-NIEVPVISAINGPAVRHAEIPLLS 129 Query: 138 DITICTEDADFFDP-HYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKNINGQTALDLGI 196 DI + ++ F D HY GG PGDG+ + ++G +A YY TG+ I A +G+ Sbjct: 130 DIVLASDTTYFMDSAHYQGGMVPGDGVHIIFPLLMGANRARYYLLTGQKIPADEAKAVGL 189 Query: 197 VSEVLPREQLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQALVADQGFQLA 249 V+EVLP ++LPRAWELA +M+ PR R S ++++ K+ + G+ LA Sbjct: 190 VNEVLPANEVLPRAWELARQLMKQPRLVRRYSRVVLTQDLKERMQGLLGYGLA 242 >B5WG86.1 B5WG86_9BURK Enoyl-CoA hydratase/isomerase Length = 252 Score = 155 bits (391), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 89/225 (39%), Positives = 134/225 (59%), Gaps = 13/225 (5%) Query: 30 GIIEVRLHTGGGPYKHNWEAHTAWSHAWSDIGRDPENEVMIISGTGDKWVIGDPEVWNTK 89 GI+ +RLH+ GGP + + H W +A+ D+GRD +N V+I++GTGD ++ + W+ Sbjct: 20 GILVMRLHSDGGPVAYRAQHHADWCNAFYDVGRDRDNRVVILTGTGDAFI--NQMAWDKS 77 Query: 90 FM---DWPKQKKLEQYHESLRLLENLIFCIDIPTIGAVNGPGT-HCELATLCDITICTED 145 DW + + E RLL NL+ I++P IGAVNGP T H ELA L DIT+ + Sbjct: 78 IENPGDWE-----DIHSEGKRLLRNLL-DIEVPVIGAVNGPATIHAELAVLSDITLASTT 131 Query: 146 ADFFD-PHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKNINGQTALDLGIVSEVLPRE 204 A F D PH T PGDG+ + ++G + Y+ TG+ ++ Q ALDLG+V+EVLP + Sbjct: 132 ATFQDFPHMNLHTVPGDGVHVVWTELLGSNRGRYFLLTGQTLSAQQALDLGVVNEVLPPD 191 Query: 205 QLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQALVADQGFQLA 249 Q+L RA ELA +++ P T + + ++ WK+ L G+ LA Sbjct: 192 QVLARARELARQLVEKPPLTVRYARTAMTVRWKRLLDEALGYGLA 236 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,829,092,524 Number of extensions: 81965667 Number of successful extensions: 154890 Number of sequences better than 1.0e-05: 1996 Number of HSP's gapped: 155906 Number of HSP's successfully gapped: 1997 Length of query: 279 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 145 Effective length of database: 1,798,280,193 Effective search space: 260750627985 Effective search space used: 260750627985 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)