BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0002.p (254 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8UQB5.1 A8UQB5_9FLAO Peptidase M15D, vanX D-ala-D-ala dipeptidase 240 8e-62 A0LZ24.1 A0LZ24_GRAFK D-alanyl-D-alanine dipeptidase EC=3.4.13.- 235 3e-60 C2G5F4.1 C2G5F4_9SPHI D-alanyl-D-alanine dipeptidase EC=3.4.13.- 229 2e-58 A2U1F8.2 A2U1F8_9FLAO D-ala-D-ala dipeptidase 228 5e-58 A0LFK7.1 A0LFK7_SYNFM VanX D-Ala-D-Ala dipeptidase. Metallo pept... 223 9e-57 >A8UQB5.1 A8UQB5_9FLAO Peptidase M15D, vanX D-ala-D-ala dipeptidase Length = 226 Score = 240 bits (613), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 108/207 (52%), Positives = 147/207 (71%) Query: 48 QKKNNLPKGFVYLDEIIPTAQYEIRYFSENNFTGSRVDGYKAPLAIFSQVGAKALKAVSD 107 Q K +LP+GFVY IIP E+RY++ NNF G +DGYK+ I ++ A+ LK V + Sbjct: 20 QDKQSLPEGFVYAKTIIPDLDIELRYYTSNNFVGDTIDGYKSNKLIITKGTAEKLKLVQE 79 Query: 108 DLARKGYILRIYDAYRPQKAVDDFVAWSQDAADIKMKQQYYPKLDKRNLFKLGFIAKKSG 167 +L + L++YD YRPQ+AV+ F+ W++D D KQQ+YP+++KRNLFK G+IA +SG Sbjct: 80 ELHLQNLCLKVYDGYRPQRAVNHFIRWARDLNDTINKQQFYPEVNKRNLFKAGYIASRSG 139 Query: 168 HTRGGTIDLTLAYAKTGNLVDMGSPYDFFGEISYYNTSLISTTQHANRKILKDAMVKHGF 227 H+RG TIDLT+ +TG +DMGSPYDFFGE S+ N I+ Q NR++L+ M+KH F Sbjct: 140 HSRGSTIDLTITNGETGEPLDMGSPYDFFGEQSWVNYKDITEEQRKNRQLLQTVMLKHNF 199 Query: 228 KPYSKEWWHFTLIKEPFPSQYFNFDVE 254 + YSKEWWHFTL EPFP YF+F+VE Sbjct: 200 RNYSKEWWHFTLRWEPFPKTYFDFEVE 226 >A0LZ24.1 A0LZ24_GRAFK D-alanyl-D-alanine dipeptidase EC=3.4.13.- Length = 226 Score = 235 bits (599), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 110/211 (52%), Positives = 151/211 (71%), Gaps = 3/211 (1%) Query: 44 PVKVQKKNNLPKGFVYLDEIIPTAQYEIRYFSENNFTGSRVDGYKAPLAIFSQVGAKALK 103 P+K Q+ +P+GFVY+DE+IP YEIRY +NF G +DGY+A AI S+ AKAL Sbjct: 19 PLKAQE---IPEGFVYIDEVIPDIIYEIRYAGSHNFVGKPIDGYEAKQAIISKPAAKALA 75 Query: 104 AVSDDLARKGYILRIYDAYRPQKAVDDFVAWSQDAADIKMKQQYYPKLDKRNLFKLGFIA 163 V +L +K ++L+++DAYRPQ+AV+ F+ W++D D MKQ +YP + K+NLF+LG+IA Sbjct: 76 KVQKELIQKDFMLKVFDAYRPQRAVNHFMEWARDKDDTLMKQDFYPDIAKKNLFQLGYIA 135 Query: 164 KKSGHTRGGTIDLTLAYAKTGNLVDMGSPYDFFGEISYYNTSLISTTQHANRKILKDAMV 223 KSGH+RG TIDLT+ VDMG YDFFGEIS++N S IS Q NR+IL+ M Sbjct: 136 TKSGHSRGSTIDLTMIGVSDCENVDMGGTYDFFGEISHHNASQISEQQKKNREILRLTMR 195 Query: 224 KHGFKPYSKEWWHFTLIKEPFPSQYFNFDVE 254 K+GF+ YS+EWWH+T EP+P+ YF+F V+ Sbjct: 196 KYGFRSYSEEWWHYTYDMEPYPATYFDFLVK 226 >C2G5F4.1 C2G5F4_9SPHI D-alanyl-D-alanine dipeptidase EC=3.4.13.- Length = 233 Score = 229 bits (583), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 110/225 (48%), Positives = 151/225 (67%), Gaps = 13/225 (5%) Query: 30 SSAAAEPAQKAVSQPVKVQKKNNLPKGFVYLDEIIPTAQYEIRYFSENNFTGSRVDGYKA 89 S A+ P QK LP GFVY+ ++IP QY+IRY +NFTG +++ Y Sbjct: 22 SCASIHPVQK-------------LPDGFVYIHKLIPNIQYDIRYAGNHNFTGKKINSYNK 68 Query: 90 PLAIFSQVGAKALKAVSDDLARKGYILRIYDAYRPQKAVDDFVAWSQDAADIKMKQQYYP 149 +AI S+ AKALK + +L RKG L+I+DAYRPQ AVD+F W+ D K+++YP Sbjct: 69 EVAILSEPAAKALKKIQRELNRKGLGLKIFDAYRPQAAVDNFKEWALIIEDTLAKKEFYP 128 Query: 150 KLDKRNLFKLGFIAKKSGHTRGGTIDLTLAYAKTGNLVDMGSPYDFFGEISYYNTSLIST 209 DKR+LFK G+IA+KS H+RG T+DLTL Y K+ +DMGSP+D+FG S+++ + ++ Sbjct: 129 DTDKRDLFKSGYIAEKSAHSRGSTVDLTLIYIKSKIELDMGSPFDYFGTPSHHDYADLTG 188 Query: 210 TQHANRKILKDAMVKHGFKPYSKEWWHFTLIKEPFPSQYFNFDVE 254 Q NR+ILK M K+GFK Y KEWWH+TL EP+P++YFNF VE Sbjct: 189 RQKENRQILKTIMEKYGFKSYRKEWWHYTLKDEPYPNEYFNFPVE 233 >A2U1F8.2 A2U1F8_9FLAO D-ala-D-ala dipeptidase Length = 222 Score = 228 bits (580), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 108/202 (53%), Positives = 140/202 (69%) Query: 53 LPKGFVYLDEIIPTAQYEIRYFSENNFTGSRVDGYKAPLAIFSQVGAKALKAVSDDLARK 112 + KGFVYL +I PT Q E+RY S+NNF G+ +DGYK I ++ A AL+ V +L +K Sbjct: 21 MAKGFVYLSDIAPTIQMELRYNSKNNFIGTPIDGYKNNCVILTKQTAFALERVQSELIKK 80 Query: 113 GYILRIYDAYRPQKAVDDFVAWSQDAADIKMKQQYYPKLDKRNLFKLGFIAKKSGHTRGG 172 G L+I+DAYRPQKAVD FV W++ D MK++YYP + K LF++G+IA KSGHTRG Sbjct: 81 GLSLKIFDAYRPQKAVDHFVKWAKVLNDTLMKKEYYPNVPKSELFQIGYIASKSGHTRGS 140 Query: 173 TIDLTLAYAKTGNLVDMGSPYDFFGEISYYNTSLISTTQHANRKILKDAMVKHGFKPYSK 232 T+DLT+ KTG +DMGSPYDFFG S+ + IS Q NR +L+ M+ F PY Sbjct: 141 TVDLTIIDLKTGKELDMGSPYDFFGVQSHPFYNDISKEQKENRMLLRRVMMAQKFIPYKN 200 Query: 233 EWWHFTLIKEPFPSQYFNFDVE 254 EWWHFTL EPF +QYFNF++E Sbjct: 201 EWWHFTLSNEPFRNQYFNFNIE 222 >A0LFK7.1 A0LFK7_SYNFM VanX D-Ala-D-Ala dipeptidase. Metallo peptidase. MEROPS family M15D Length = 234 Score = 223 bits (569), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 101/214 (47%), Positives = 148/214 (69%), Gaps = 3/214 (1%) Query: 44 PVKVQKKNNLPKGFVYLDEIIPTAQYEIRYFSENNFTGSRVDGYKAPLAIFSQVGAKALK 103 P+ V ++ LP GFVY+DE++P E+RY +E+NF G+ +DGY+ I ++ A+ALK Sbjct: 21 PLAVAQEAGLPDGFVYVDEVVPGVNVELRYHTEHNFVGTPIDGYRGARCILTREAAEALK 80 Query: 104 AVSDDLARKGYILRIYDAYRPQKAVDDFVAWSQDAADIKMKQQYYPKLDKRNLFKLGFIA 163 V +DL G L+IYD YRPQ+AV+ FV W+QD +D +MKQ++YP +DK +LFK G+IA Sbjct: 81 GVQEDLKPFGLGLKIYDGYRPQRAVNHFVRWAQDLSDTRMKQEFYPGVDKADLFKEGYIA 140 Query: 164 KKSGHTRGGTIDLT---LAYAKTGNLVDMGSPYDFFGEISYYNTSLISTTQHANRKILKD 220 +KS H+RG T+DLT L A++ +DMG +DFFG S + L++ ++ A+R +L+ Sbjct: 141 EKSSHSRGSTVDLTIVPLEAAESEAELDMGGAFDFFGPESRPDWPLLTPSRRAHRMLLQT 200 Query: 221 AMVKHGFKPYSKEWWHFTLIKEPFPSQYFNFDVE 254 M HGF+PY KEWWH+TL EP+P F+F V+ Sbjct: 201 LMRNHGFQPYPKEWWHYTLKNEPYPDTVFDFPVQ 234 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,543,984,754 Number of extensions: 63760848 Number of successful extensions: 143184 Number of sequences better than 1.0e-05: 323 Number of HSP's gapped: 142950 Number of HSP's successfully gapped: 324 Length of query: 254 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 121 Effective length of database: 1,807,701,208 Effective search space: 218731846168 Effective search space used: 218731846168 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)