BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0017.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1TT66.1 C1TT66_9SPHI Beta-hydroxyacid dehydrogenase, 3-hydroxyi... 207 8e-52 C1ZWE1.1 C1ZWE1_RHOMR Beta-hydroxyacid dehydrogenase, 3-hydroxyi... 188 4e-46 Q08S14.1 Q08S14_STIAU 6-phosphogluconate dehydrogenase, NAD-binding 182 3e-44 Q1D363.1 Q1D363_MYXXD 3-hydroxyisobutyrate dehydrogenase family ... 182 4e-44 B3DXR0.1 B3DXR0_METI4 3-hydroxyisobutyrate dehydrogenase or rela... 177 6e-43 >C1TT66.1 C1TT66_9SPHI Beta-hydroxyacid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase Length = 294 Score = 207 bits (527), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 110/249 (44%), Positives = 159/249 (63%), Gaps = 4/249 (1%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVVGSEDFL 82 +A Y L V+NRTA KA+PL GA DA+T GGIV +++ DD A+E + S + + Sbjct: 23 EAGYELVVWNRTASKAEPLTKLGATVVENAIDAITPGGIVFSVLADDAAVEELF-SMELV 81 Query: 83 ERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQE 142 E+LG G+HVSMST+SP+T R+LA +H +G+ YV AP+F RPEA AK I ++G Sbjct: 82 EKLGKDGVHVSMSTISPETSRQLAQVHEWYGAHYVGAPIFARPEAVRAKVGNICLSGNAG 141 Query: 143 AKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPK 202 AKER++P++E +G+FDFG++ GAA ++KL GNF+I + + EA MAE+ G+ + Sbjct: 142 AKERIKPIVENF-VKGVFDFGDDPGAANVIKLAGNFMIACSLEMMGEAFTMAEKNGISRQ 200 Query: 203 AVVNMLTSTLFPAPIYQSYGKMIAEKTANISESKIP--QKDVGLFLAAAQNAQFPAPLAG 260 ++ MLTSTLF API+Q+YGK++A T + P KD+ L L A + P P A Sbjct: 201 SIYEMLTSTLFAAPIFQNYGKLVASNTYEPVAFRFPLGLKDINLTLQTASDVNAPMPFAD 260 Query: 261 QLLDMLESG 269 + + SG Sbjct: 261 IIRNRFISG 269 >C1ZWE1.1 C1ZWE1_RHOMR Beta-hydroxyacid dehydrogenase, 3-hydroxyisobutyrate dehydrogenase Length = 297 Score = 188 bits (478), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 115/254 (45%), Positives = 156/254 (61%), Gaps = 9/254 (3%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVV-GSEDF 81 +A Y + VYNRT +KA+ LV++GA A+ P +A T G+V+T+V DD ALE+VV G + Sbjct: 22 KAGYEVVVYNRTPEKAEALVSEGARLASTPGEAARTTGLVLTMVADDAALEAVVEGPDGL 81 Query: 82 LERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQ 141 L L GGIHV+MST+SP+ L H G +V APVFGRP+AAAA +L I AGP Sbjct: 82 LAHLPAGGIHVAMSTISPELSASLTRRHQERGQFFVGAPVFGRPDAAAAARLRIVAAGPT 141 Query: 142 EAKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDP 201 EA ER RPVLE +G++ +F G E A LVKL GNFL+ S +L EA + + GLDP Sbjct: 142 EAIERCRPVLEVLGSQ-LFVVGAEPAQAHLVKLAGNFLLASMLEALSEAFALTRKAGLDP 200 Query: 202 KAVVNMLTSTLFPAPIYQSYGKMIAEK--TANISESKIPQKDVGLFLAAAQNAQFPAPLA 259 + ++ + LF +PIY +YG++IA + T ++ KDV L AA Q P PLA Sbjct: 201 QQFFEIIDA-LFGSPIYHAYGQLIASQRYTPPGFTIRLGLKDVRLARQAAHALQVPMPLA 259 Query: 260 ----GQLLDMLESG 269 L++ L +G Sbjct: 260 SLVENHLIEALAAG 273 >Q08S14.1 Q08S14_STIAU 6-phosphogluconate dehydrogenase, NAD-binding Length = 295 Score = 182 bits (462), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 111/250 (44%), Positives = 149/250 (59%), Gaps = 4/250 (1%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALES-VVGSEDF 81 A + L VYNRT KA+P A+GA A P +A V +++ DD ALE+ V G E Sbjct: 21 NAGHELVVYNRTRAKAEPFQAKGARIAGTPQEAARGAEAVFSILADDPALEAAVFGGEGL 80 Query: 82 LERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQ 141 L L G IH+S ST+S +LA HA+ G YV APVFGRPEAA AKQLW+ AG + Sbjct: 81 LSGLARGAIHISSSTISVALSERLAAEHAQAGQGYVSAPVFGRPEAAEAKQLWVLAAGAK 140 Query: 142 EAKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDP 201 ERVRP+LEA+G RG+ GE+A +A +VKL GNFLI S +L EA +A++ G++P Sbjct: 141 ADVERVRPLLEAIG-RGLTVLGEKASSANVVKLSGNFLIASMVEALGEAFALAQKSGVEP 199 Query: 202 KAVVNMLTSTLFPAPIYQSYGKMIAEKTANIS--ESKIPQKDVGLFLAAAQNAQFPAPLA 259 K + + S +PI++ Y +IA + ++ KD+GL L AA +AQ P PLA Sbjct: 200 KTFLEVFQSVFAKSPIFERYASLIAGRQFQPPGFALRLGLKDIGLVLEAAGDAQVPMPLA 259 Query: 260 GQLLDMLESG 269 + D L G Sbjct: 260 SLVKDHLLGG 269 >Q1D363.1 Q1D363_MYXXD 3-hydroxyisobutyrate dehydrogenase family protein Length = 296 Score = 182 bits (461), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 105/249 (42%), Positives = 151/249 (60%), Gaps = 4/249 (1%) Query: 24 ARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALES-VVGSEDFL 82 A + LTV+NRT KA PL +GA A PA+A +V +++ DD A+ S V G + L Sbjct: 22 AGHELTVWNRTESKAAPLKEKGARVARSPAEAARGAEVVFSMLADDAAVTSAVFGQDGLL 81 Query: 83 ERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQE 142 L PG +HVS ST+S +LA+ HA G Y+ APVFGRP AAA+KQLW+ AGP++ Sbjct: 82 AGLAPGAVHVSSSTLSVALSERLAEAHASAGQRYLAAPVFGRPTAAASKQLWVVAAGPKQ 141 Query: 143 AKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPK 202 +R RP+LE +G RG+ G+ A AA +VKL GNFLI S +L E+ + + G++PK Sbjct: 142 DMDRCRPLLETLG-RGLTVLGDRAPAANVVKLSGNFLIASMMEALAESFALTRKSGIEPK 200 Query: 203 AVVNMLTSTLFPAPIYQSYGKMIAEKTANIS--ESKIPQKDVGLFLAAAQNAQFPAPLAG 260 + + S API++ Y ++IAE+ + + + ++ KDV L L AA+ A+ P PLA Sbjct: 201 VFLEVFQSVFARAPIFEGYAQLIAEEKYSPAGFKMRLGLKDVELVLGAARTAEVPMPLAS 260 Query: 261 QLLDMLESG 269 + D G Sbjct: 261 LVKDQFLGG 269 >B3DXR0.1 B3DXR0_METI4 3-hydroxyisobutyrate dehydrogenase or related beta-hydroxyacid dehydrogenase Length = 307 Score = 177 bits (450), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 101/251 (40%), Positives = 148/251 (58%), Gaps = 12/251 (4%) Query: 28 LTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVVG-SEDFLERLG 86 L++YNRT KA+PLV+ GA + P +A +V+T+V +DEAL V G +E + L Sbjct: 40 LSIYNRTPHKAEPLVSMGAKIFSCPREAAQNCDVVITMVANDEALLEVTGGAEGLINGLK 99 Query: 87 PGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQEAKER 146 GGIH+SMSTVSP T + + LHA + V APVFGRP AAA K+LWI AGP EA R Sbjct: 100 EGGIHLSMSTVSPRTIQVIQSLHASKNQIVVSAPVFGRPNAAAKKELWIVTAGPLEAVRR 159 Query: 147 VRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPKAVVN 206 P+ +A+G RG + GEE A +VK++GNFLI+S +L E+ +A++ + + +++ Sbjct: 160 CHPIFQALG-RGYSNLGEETIKANIVKILGNFLIMSVVEALAESFCVAQKANISAQDLLS 218 Query: 207 MLTSTLFPAPIYQSYGKMIAEKTANISES----KIPQKDVGLFLAAAQNAQFPAPL---- 258 + LF +P+Y++YG++I K+ SE K+ KD L ++ P P Sbjct: 219 AVNQALFRSPLYENYGQLICNKS--FSEPGFTLKLGFKDANLVRELVESYLSPMPFLDIV 276 Query: 259 AGQLLDMLESG 269 G+ L L G Sbjct: 277 YGRFLSALNHG 287 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,459,111,400 Number of extensions: 60214201 Number of successful extensions: 170670 Number of sequences better than 1.0e-05: 2678 Number of HSP's gapped: 167083 Number of HSP's successfully gapped: 2678 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)