BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0012.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9B7V5.1 A9B7V5_HERA2 Radical SAM domain protein 276 2e-72 C4RCQ8.1 C4RCQ8_9ACTO Putative uncharacterized protein 271 4e-71 B9XL15.1 B9XL15_9BACT Radical SAM domain-containing protein 271 5e-71 A3ITC0.1 A3ITC0_9CHRO Radical SAM domain protein 268 4e-70 Q1D8C3.1 Q1D8C3_MYXXD Radical SAM domain protein 267 1e-69 >A9B7V5.1 A9B7V5_HERA2 Radical SAM domain protein Length = 288 Score = 276 bits (706), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 138/282 (48%), Positives = 179/282 (63%), Gaps = 2/282 (0%) Query: 1 MRAVLYYRGALTSCNYDCPYCPFGKTRDSAERLAKDREELEAFVRWASAQGAEGHCLSIF 60 M+ + YRG L+SCNY C YCPF K +++A A D L+ FV W + Q LSIF Sbjct: 1 MKLAILYRGPLSSCNYGCSYCPFAKHQETAAEHAHDEAALQRFVAWVAQQIQLE--LSIF 58 Query: 61 FNPYGEALIHRWYKEAMITLSNLDHIDKVAIQTNLSAGLDFAARLNPHKAAFWATYHPGQ 120 F P+GEALI R Y+ A++ L+NL H+ KVAIQTNLSA LD+A + WATYHP Q Sbjct: 59 FTPWGEALIRRRYQRAIVELANLPHVQKVAIQTNLSARLDWAEDCPKQRLGLWATYHPTQ 118 Query: 121 VTEQRFLAQCMAVYEQGIPLSVGSVGIRSSFEAIASLRAALPEEVYLWVNAYKDKPNYYT 180 +RFLA+C +GIP+SVG VG+ IA+LR ALP+++YLW+NAYK + YY+ Sbjct: 119 TPRERFLARCHEAITRGIPISVGVVGMHEHQAEIAALRKALPKQIYLWINAYKREVGYYS 178 Query: 181 GEDIAFLSGIDPYFMLNARDYESLGKACGAGSSVFYVQGPGQVKRCYKDRGVIGNLYRDG 240 E+I L+ IDP F +N + S G+AC G SV V G G ++RC+ R IGN+Y Sbjct: 179 PEEITQLTAIDPLFPINNTRHPSEGRACATGESVISVDGDGTMRRCHFVREPIGNIYNVD 238 Query: 241 LEGLSAERSCRMKVCDCYIGYIHMPELQLQQVYGAGLLERIP 282 + R C C C+IGYIHMP L LQQV+G G+LERIP Sbjct: 239 WQTALQPRVCANATCGCHIGYIHMPHLGLQQVFGQGMLERIP 280 >C4RCQ8.1 C4RCQ8_9ACTO Putative uncharacterized protein Length = 294 Score = 271 bits (694), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 134/284 (47%), Positives = 176/284 (61%), Gaps = 4/284 (1%) Query: 5 LYYRGALTSCNYDCPYCPFGKTRDSAERLAKDREELEAFVRWASAQGAEGHCLSIFFNPY 64 + YRG L SCNYDCPYCPF K D + LA DR LE F W +A LS+ F P+ Sbjct: 15 ILYRGPLASCNYDCPYCPFAKRNDPPQVLAADRAALERFTGWVAATTDVR--LSVLFTPW 72 Query: 65 GEALIHRWYKEAMITLSNLDHIDKVAIQTNLSAGLDFAARLNPHKAAFWATYHPGQVTEQ 124 GE L RWY+EAM+ LS+L H+D+V IQTNL+A +D+ A +P +AA W TYHPGQVT + Sbjct: 73 GEGLTRRWYREAMVALSHLPHVDRVVIQTNLAARVDWLADADPTRAALWTTYHPGQVTRE 132 Query: 125 RFLAQCMAVYEQGIPLSVGSVGIRSSFEAIASLRAALPEEVYLWVNAYKDKPNYYTGEDI 184 RFLA+C + E G+ SVG VG+ + +LRAALP +VYLWVNA + + +Y + Sbjct: 133 RFLARCARLAELGVRFSVGVVGLPEHLDEARALRAALPPQVYLWVNAAEHR--HYDAAEE 190 Query: 185 AFLSGIDPYFMLNARDYESLGKACGAGSSVFYVQGPGQVKRCYKDRGVIGNLYRDGLEGL 244 A + +DP F + R + SLG+ C AG + V+G G V+RC+ IGNLY Sbjct: 191 ATWTELDPLFGYSVRPHLSLGRPCHAGETAISVRGDGTVRRCHFVDQPIGNLYDGSWRAA 250 Query: 245 SAERSCRMKVCDCYIGYIHMPELQLQQVYGAGLLERIPCGVREG 288 R+C CDC+IGY+H+ L L+ V+ GLLERIP G G Sbjct: 251 LRPRTCVNGACDCHIGYVHLKPLGLRDVFAGGLLERIPAGWPRG 294 >B9XL15.1 B9XL15_9BACT Radical SAM domain-containing protein Length = 288 Score = 271 bits (693), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 130/282 (46%), Positives = 176/282 (62%), Gaps = 2/282 (0%) Query: 1 MRAVLYYRGALTSCNYDCPYCPFGKTRDSAERLAKDREELEAFVRWASAQGAEGHCLSIF 60 M + YRG L+SCNY C YCPF K +++A +LA D + L FV W S++ G +SI Sbjct: 1 MNLSILYRGPLSSCNYGCDYCPFAKRQETAAQLAIDEKALTRFVSWVSSRS--GDRISIL 58 Query: 61 FNPYGEALIHRWYKEAMITLSNLDHIDKVAIQTNLSAGLDFAARLNPHKAAFWATYHPGQ 120 F P+GEALI RWY+ A + LS+L H+ K+AIQTNLS LD+ N + A W T+HP + Sbjct: 59 FTPWGEALIRRWYQRAFVELSHLPHVKKIAIQTNLSCRLDWIEDCNKSRIALWTTFHPSE 118 Query: 121 VTEQRFLAQCMAVYEQGIPLSVGSVGIRSSFEAIASLRAALPEEVYLWVNAYKDKPNYYT 180 +T +RF+AQC + ++G+ SVG VG++ I LR L VYLW+NAYK P+YYT Sbjct: 119 ITRKRFVAQCHKLDQRGVRYSVGVVGLKEHASEIDELRRELAPNVYLWINAYKRVPDYYT 178 Query: 181 GEDIAFLSGIDPYFMLNARDYESLGKACGAGSSVFYVQGPGQVKRCYKDRGVIGNLYRDG 240 E I + IDP F +N + S G C AG SV V G G ++RC+ R IGN+Y G Sbjct: 179 PELIKRFTSIDPLFPVNNTRHPSAGLECHAGESVISVDGEGVIRRCHFIRDPIGNIYEPG 238 Query: 241 LEGLSAERSCRMKVCDCYIGYIHMPELQLQQVYGAGLLERIP 282 E + R+C C C+IGY+HM +L L V+G G+LER+P Sbjct: 239 FEKVLLPRTCPQPSCGCHIGYVHMNKLNLYDVFGDGVLERVP 280 >A3ITC0.1 A3ITC0_9CHRO Radical SAM domain protein Length = 285 Score = 268 bits (686), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 125/282 (44%), Positives = 174/282 (61%), Gaps = 2/282 (0%) Query: 1 MRAVLYYRGALTSCNYDCPYCPFGKTRDSAERLAKDREELEAFVRWASAQGAEGHCLSIF 60 M + YRG+L SCNY C YCPF K + SA LAKD++EL F+ W + + H SI Sbjct: 1 MHLSILYRGSLISCNYGCQYCPFAKKQQSAIELAKDKQELTKFINWI--EQNQQHDFSIL 58 Query: 61 FNPYGEALIHRWYKEAMITLSNLDHIDKVAIQTNLSAGLDFAARLNPHKAAFWATYHPGQ 120 F P+GEALIH WY++A+I L+NL ++ K AIQTNLS LD+ + +K A WAT+HP Sbjct: 59 FTPWGEALIHDWYQQALIRLTNLPNVKKAAIQTNLSCKLDWVEDCDKNKLALWATFHPEW 118 Query: 121 VTEQRFLAQCMAVYEQGIPLSVGSVGIRSSFEAIASLRAALPEEVYLWVNAYKDKPNYYT 180 V R+L+QC+ +Y + + S G VG IA+LR LP++VYLW+NA K + + Sbjct: 119 VKRDRYLSQCLELYRRQVSFSAGVVGFPQFKSEIATLRQELPDDVYLWINAVKKELPNMS 178 Query: 181 GEDIAFLSGIDPYFMLNARDYESLGKACGAGSSVFYVQGPGQVKRCYKDRGVIGNLYRDG 240 D+ F IDP F LN Y S ++C AG SV V G G ++RC+ + IGN+Y Sbjct: 179 KADLEFFEAIDPLFPLNTHHYPSRDRSCKAGKSVISVDGDGNMRRCHFIKQPIGNIYHSN 238 Query: 241 LEGLSAERSCRMKVCDCYIGYIHMPELQLQQVYGAGLLERIP 282 E + +R C + C C+IGY+H+ L+L +V+G +LERIP Sbjct: 239 WESVLGDRLCTNETCHCHIGYVHLDYLELDKVFGERILERIP 280 >Q1D8C3.1 Q1D8C3_MYXXD Radical SAM domain protein Length = 294 Score = 267 bits (682), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 130/283 (45%), Positives = 177/283 (62%), Gaps = 3/283 (1%) Query: 1 MRAVLYYRGALTSCNYDCPYCPFGKTRDSAERLAKDREELEAFVRWASAQGAEGHCLSIF 60 M+ + YRG L+SCNY C YCPFGK + + E LAKDR +LE FV W A+ + +S+F Sbjct: 1 MKLTVLYRGPLSSCNYGCEYCPFGKWKHTDEELAKDRADLERFVAWVEARTQD--TVSVF 58 Query: 61 FNPYGEALIHRWYKEAMITLSNLDHIDKVAIQTNLSAGLDFAARLNPHKAAFWATYHPGQ 120 F P+GEALI WY+EA+ LS+L H++++AIQTNLS LD+ A + K WATYHP Sbjct: 59 FTPWGEALIWPWYQEALARLSHLPHVERLAIQTNLSCKLDWVAGVRADKLGIWATYHPEW 118 Query: 121 VTEQRFLAQCMAVYEQGIPLSVGSVGIRSSFEAIASLRAALPEEVYLWVNAYKD-KPNYY 179 RF+AQC + E G+ SVG VG E +LRA LP + YLW+NA KD + Y Sbjct: 119 TKRHRFVAQCAKLTELGVRHSVGVVGFLRFTEEAEALRAELPADTYLWINAVKDGQEEPY 178 Query: 180 TGEDIAFLSGIDPYFMLNARDYESLGKACGAGSSVFYVQGPGQVKRCYKDRGVIGNLYRD 239 T ED+A + +DP F +N + SLG+AC G SV V G G +RC+ IGN+Y Sbjct: 179 TAEDVARFTRLDPLFPVNNTRHPSLGRACRGGESVISVDGEGTARRCHFIEEAIGNIYAP 238 Query: 240 GLEGLSAERSCRMKVCDCYIGYIHMPELQLQQVYGAGLLERIP 282 + R C + C C+IGY+H+ L+L +V+G+G+LER+P Sbjct: 239 DFDSALRPRPCAKQTCGCHIGYVHLEYLELDRVFGSGILERVP 281 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,788,504,279 Number of extensions: 73796699 Number of successful extensions: 149915 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 149798 Number of HSP's successfully gapped: 46 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)