BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0005.p (303 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBB3.1 B3KBB3_9BACI 1-phosphofructokinase 376 e-102 Q71X93.1 Q71X93_LISMF 1-phosphofructokinase EC=2.7.1.56 358 5e-97 Q4EJJ9.1 Q4EJJ9_LISMO 1-phosphofructokinase EC=2.7.1.56 358 5e-97 Q8Y4U5.1 Q8Y4U5_LISMO Fructose-1-phosphate kinase 357 6e-97 Q4ES33.1 Q4ES33_LISMO 1-phosphofructokinase EC=2.7.1.56 357 6e-97 >B3KBB3.1 B3KBB3_9BACI 1-phosphofructokinase Length = 303 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 192/302 (63%), Positives = 223/302 (73%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYTVTLNPSIDYIVEVE + LGGLNRM RD+K PGGKGINVSRVLNQLG NTA GFLG Sbjct: 1 MIYTVTLNPSIDYIVEVEHIELGGLNRMTRDMKFPGGKGINVSRVLNQLGADNTAIGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTGRYI + L +E I +DFV +A+DTRIN+KLK ETEING GP IR Sbjct: 61 GFTGRYIDEMLGKEGIRTDFVNIADDTRINVKLKHGGETEINGLGPDIREDETKELLHKL 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 GDI+V FY RLI C+ AEFV+DTTG+AL ALA +PLLVKP Sbjct: 121 SQLEAGDIVVLSGSVPPSLGSDFYGRLIEACRKGGAEFVMDTTGEALMRALAYKPLLVKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELAEL+GV I SR++++ YGRRL+E GA++VL+SMAGEGALFI+ VY A+ P G Sbjct: 181 NHHELAELYGVVIDSREQLVEYGRRLLEKGAQHVLISMAGEGALFITADRVYHAHVPPGT 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 V+NSVGAGDSMIAGFVGTL+ +EAFRTGVASGSATAFSDDLA R I +L ++I Sbjct: 241 VRNSVGAGDSMIAGFVGTLAKGQGPIEAFRTGVASGSATAFSDDLAAREAIDELLPLVRI 300 Query: 301 EQ 302 + Sbjct: 301 TE 302 >Q71X93.1 Q71X93_LISMF 1-phosphofructokinase EC=2.7.1.56 Length = 307 Score = 358 bits (918), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 176/303 (58%), Positives = 220/303 (72%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYT+TLNPSIDYIV+++ L LGGLNRM++D KLPGGKGINVSRVLNQL V + ATGFLG Sbjct: 1 MIYTITLNPSIDYIVQIDQLNLGGLNRMKQDYKLPGGKGINVSRVLNQLNVPSLATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG +I WL E + + FVTV +DTRINIKLK EETEING GP I Sbjct: 61 GFTGNFIKDWLQNEGVKTGFVTVKDDTRINIKLKHGEETEINGLGPAISEKEINGFLKVM 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 DI++ FY+++I CK KKAEF+IDTTGQ L DAL P+L+KP Sbjct: 121 DKVTANDIVILSGSVPPSLGNDFYNKIIQICKEKKAEFMIDTTGQELLDALPNHPILIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELA+LFGV +GS +E+I YG++ +E GA++V+VSMAG+GAL + + VY A+A KG+ Sbjct: 181 NHHELADLFGVKLGSVEELIPYGKKCLELGAQHVIVSMAGDGALLFTGEDVYFADALKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 +KNSVGAGDSMIAGFVGT T D ++AF GVA+G ATAFS DLA + LI +L Q++I Sbjct: 241 LKNSVGAGDSMIAGFVGTFDKTRDPVKAFAAGVATGGATAFSTDLAQKELIDELLPQVKI 300 Query: 301 EQL 303 ++ Sbjct: 301 TKI 303 >Q4EJJ9.1 Q4EJJ9_LISMO 1-phosphofructokinase EC=2.7.1.56 Length = 307 Score = 358 bits (918), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 176/303 (58%), Positives = 220/303 (72%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYT+TLNPSIDYIV+++ L LGGLNRM++D KLPGGKGINVSRVLNQL V + ATGFLG Sbjct: 1 MIYTITLNPSIDYIVQIDQLNLGGLNRMKQDYKLPGGKGINVSRVLNQLNVPSLATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG +I WL E + + FVTV +DTRINIKLK EETEING GP I Sbjct: 61 GFTGNFIKDWLQNEGVKTGFVTVKDDTRINIKLKHGEETEINGLGPAISEKEINGFLKVM 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 DI++ FY+++I CK KKAEF+IDTTGQ L DAL P+L+KP Sbjct: 121 DKVTANDIVILSGSVPPSLGNDFYNKIIQICKEKKAEFMIDTTGQELLDALPNHPILIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELA+LFGV +GS +E+I YG++ +E GA++V+VSMAG+GAL + + VY A+A KG+ Sbjct: 181 NHHELADLFGVKLGSVEELIPYGKKCLELGAQHVIVSMAGDGALLFTGEDVYFADALKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 +KNSVGAGDSMIAGFVGT T D ++AF GVA+G ATAFS DLA + LI +L Q++I Sbjct: 241 LKNSVGAGDSMIAGFVGTFDKTRDPVKAFAAGVATGGATAFSTDLAQKELIDELLPQVKI 300 Query: 301 EQL 303 ++ Sbjct: 301 TKI 303 >Q8Y4U5.1 Q8Y4U5_LISMO Fructose-1-phosphate kinase Length = 307 Score = 357 bits (917), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 176/303 (58%), Positives = 220/303 (72%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYT+TLNPSIDYIV+++ L LGGLNRM++D KLPGGKGINVSRVLNQL V + ATGFLG Sbjct: 1 MIYTITLNPSIDYIVQIDQLNLGGLNRMKQDYKLPGGKGINVSRVLNQLNVPSLATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG +I WL E + + FVTV +DTRINIKLK EETEING GP I Sbjct: 61 GFTGSFIKDWLQNEGVKTGFVTVKDDTRINIKLKHGEETEINGLGPAISEKEINEFLKVM 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 DI++ FY+++I CK KKAEF+IDTTGQ L DAL P+L+KP Sbjct: 121 DKVTANDIVILSGSVPPSLGNDFYNKIIQICKEKKAEFMIDTTGQELLDALPNRPILIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELA+LFGV +GS +E+I YG++ +E GA++V+VSMAG+GAL + + VY A+A KG+ Sbjct: 181 NHHELADLFGVKLGSVEELIPYGKKCLELGAQHVIVSMAGDGALLFTGEDVYFADALKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 +KNSVGAGDSMIAGFVGT T D ++AF GVA+G ATAFS DLA + LI +L Q++I Sbjct: 241 LKNSVGAGDSMIAGFVGTFDKTRDPVKAFAAGVATGGATAFSTDLAQKELIDELLPQVKI 300 Query: 301 EQL 303 ++ Sbjct: 301 TKI 303 >Q4ES33.1 Q4ES33_LISMO 1-phosphofructokinase EC=2.7.1.56 Length = 307 Score = 357 bits (917), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 176/303 (58%), Positives = 220/303 (72%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYT+TLNPSIDYIV+++ L LGGLNRM++D KLPGGKGINVSRVLNQL V + ATGFLG Sbjct: 1 MIYTITLNPSIDYIVQIDQLNLGGLNRMKQDYKLPGGKGINVSRVLNQLNVPSLATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG +I WL E + + FVTV +DTRINIKLK EETEING GP I Sbjct: 61 GFTGSFIKDWLQNEGVKTGFVTVKDDTRINIKLKHGEETEINGLGPAISEKEINEFLKVM 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 DI++ FY+++I CK KKAEF+IDTTGQ L DAL P+L+KP Sbjct: 121 DKVTANDIVILSGSVPPSLGNDFYNKIIQICKEKKAEFMIDTTGQELLDALTNRPILIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELA+LFGV +GS +E+I YG++ +E GA++V+VSMAG+GAL + + VY A+A KG+ Sbjct: 181 NHHELADLFGVKLGSVEELIPYGKKCLELGAQHVIVSMAGDGALLFTGEDVYFADALKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 +KNSVGAGDSMIAGFVGT T D ++AF GVA+G ATAFS DLA + LI +L Q++I Sbjct: 241 LKNSVGAGDSMIAGFVGTFDKTRDPVKAFAAGVATGGATAFSTDLAQKELIDELLPQVKI 300 Query: 301 EQL 303 ++ Sbjct: 301 TKI 303 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,517,025,518 Number of extensions: 58734267 Number of successful extensions: 165462 Number of sequences better than 1.0e-05: 1248 Number of HSP's gapped: 163859 Number of HSP's successfully gapped: 1445 Length of query: 303 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 168 Effective length of database: 1,788,859,178 Effective search space: 300528341904 Effective search space used: 300528341904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)