BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0498.0011.p (510 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KF69.1 B3KF69_9BACI Extracellular solute-binding protein family 1 281 8e-74 C5C4Z8.1 C5C4Z8_9MICO Extracellular solute-binding protein family 1 260 2e-67 C0UXG2.1 C0UXG2_9BACT Carbohydrate ABC transporter substrate-bin... 160 3e-37 B1DFA2.1 B1DFA2_9BACL Extracellular solute-binding protein family 1 153 4e-35 B3KF70.1 B3KF70_9BACI Putative uncharacterized protein 149 6e-34 >B3KF69.1 B3KF69_9BACI Extracellular solute-binding protein family 1 Length = 383 Score = 281 bits (720), Expect = 8e-74, Method: Compositional matrix adjust. Identities = 156/383 (40%), Positives = 228/383 (59%), Gaps = 12/383 (3%) Query: 1 MRKKNRVMAWTAL--AMTVALFSGWGG---GSNTPKTAE--GDASAKSAAGPVDIEAAEI 53 M+K ++ +L AM A G G G+ T TA+ + + + + PV ++ E Sbjct: 1 MKKLGTLLVAVSLVGAMLTACSGGNSGDNPGNQTGATAKPGAETTEQPSGKPVKLDIIET 60 Query: 54 SWGTSLPADDFIKKALDEQVGVNLKLTLVGDQNDYENQMNVRAASGNLPDLLMATSKAHL 113 P D IK+ LD+ + ++L LT+ +DY NQ+NVR +SGN PDL M + L Sbjct: 61 GNNLPSPDKDMIKQELDKALNIDLNLTVYPAADDYNNQLNVRMSSGNFPDLFM-VDRQQL 119 Query: 114 QKLAQSGSLLDLTPYLDKLPEYTKFVGDEVLKKGILDSKQYALPKTGQSVSYTYWIRKDW 173 + ++ G LLDLTPY+DKL + F+G++ +KKG++D K Y + K+ Q T WIRKDW Sbjct: 120 IQFSKQGLLLDLTPYMDKLEQTVAFIGEDSVKKGMVDGKVYGISKSPQIPYNTLWIRKDW 179 Query: 174 LDNLQLQPPATVDELLEVAKAFTTNDPDKNGKNDTFGLTGTNFQTFDPIFGAYGLTNIFD 233 LD L+L+ P TV++L VA+AFT NDPD NGK DTFGLTG+ F +FGA+G+ Sbjct: 180 LDKLKLEVPTTVEQLASVAEAFTKNDPDGNGKPDTFGLTGSKLSAFSTVFGAFGVGM--- 236 Query: 234 TSQLFVRDGKVVSTFYQPEMKEALTTIKGFLDAGVVDPEIVSNKGTMAIDKAFQGKVGII 293 + +DGKVV++ ++P MK+AL IK + +G VDPE+++N G +KA +G+ GI+ Sbjct: 237 PGNFYEKDGKVVNSLHEPAMKDALAFIKDMIGSGSVDPELLANSGLQHQEKAIKGQAGIV 296 Query: 294 NTEWAQIMKDDKVAVWKGANPKAEWIQLAPPKGPTGIANANSLNIGASSGLWVIPKKLES 353 +W + K+ V K NP AEW+Q+A P G TG S +IGA+ G + IPK LE Sbjct: 297 YIDWPNMTKEQFVEQIKTVNPNAEWLQIAAPSG-TGGQFDGSWDIGAAPGRYAIPKALEK 355 Query: 354 EPEKLNKVLELLNYVSTKEGNRL 376 +PEKL +V +LL + R Sbjct: 356 DPEKLQRVFDLLTIFRIRTEARC 378 >C5C4Z8.1 C5C4Z8_9MICO Extracellular solute-binding protein family 1 Length = 492 Score = 260 bits (665), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 167/513 (32%), Positives = 256/513 (49%), Gaps = 37/513 (7%) Query: 3 KKNRVMAWTALAMTVALFSGW---GGGSNTPKTAEGDASAKSAAGPVDIEAAEISWGTSL 59 ++ R ++ A++ V L +G GGG N E + G D+ T + Sbjct: 4 RRGRRLSVAAISAIVLLSAGCSVLGGGDND----EERVLRINYGGSADV--------TEI 51 Query: 60 PADDFIKKALDEQVGVNLKLTLVGDQNDYENQMNVRAASGNLPDLLMATSKAHLQKLAQS 119 PAD+ I L+E G + + + A PDL + TS++ +++ + Sbjct: 52 PADNPIADKLEEITGYEIVV------EKSPEDLGAALAGDQAPDLFL-TSRSQVRQFDEQ 104 Query: 120 GSLLDLTPYLDKLPEYTKFVGDEVLKKGILDSKQYALPKTGQSVSYT-YWIRKDWLDNLQ 178 G LLDLT + D+L +Y +VG E + +LD + ++ + + SY WIR DWL+NL Sbjct: 105 GLLLDLTDHRDELSDYESYVGSENVDATLLDGRLVSVVRKPREFSYNGLWIRADWLENLG 164 Query: 179 LQPPATVDELLEVAKAFTTNDPDKNGKNDTFGLTGTNFQTFDPIFGAYGLTNIFDTSQLF 238 L P T E +V +AFTT DPD NG DT G+TG+ TF +FGAYG L+ Sbjct: 165 LAMPTTTAEFHDVLQAFTTGDPDGNGAADTVGITGSGMGTFSAVFGAYGTGG---PGTLY 221 Query: 239 VRDGKVVSTFYQPEMKEALTTIKGFLDAGVVDPEIVSNKGTMAIDKAFQGKVGIINTEWA 298 +V + P + E LT I +VDP++ S + A D+AFQG G++ W Sbjct: 222 ADGPAIVDGYNDPAVIEPLTYINTLFAEQLVDPDLFSIEIAEARDRAFQGSAGVVAISWD 281 Query: 299 QIMKDDKVAVWKGANPKAEWIQLAPPKGPTGIANANSLNIGASSGLWVIPKKLESEPEKL 358 Q+ K + V + A P ++W ++ GP G G + IP + +P+K+ Sbjct: 282 QMTKPEFVEAQQAAQPGSDWQMISVLDGPDGPGAQPGSPFGV---VQAIPASVGDDPDKV 338 Query: 359 NKVLELLNYVSTKEGNRLVQYGVENTHYTLEGDTVKITDKGRSDASFTWLYQFTGRPETD 418 ++ LLNY+ST +G+RLV +GVE + ++G+ V ++ S+ + YQ TGR E Sbjct: 339 EALITLLNYISTPDGSRLVMFGVEGETFEMDGEEVVPLPALDTEGSYFFAYQMTGRDEDT 398 Query: 419 YLKSKFSNLA----AYIDFEANLPRIQTLDGFVTSPPGYNPADANRFIEEEMIKFVYGKA 474 YL +KF + A A +D E +I D V P GYN ADA RF +E+++ FV G+ Sbjct: 399 YLATKFPDEAWAWQACMDRE----QIVLYDALVAPPDGYNVADAARFADEQIVLFVTGER 454 Query: 475 KIDSYSKFLETLENTFSYKNYLDTAISQLNELG 507 ++ Y FL L F Y Y+ +A QL LG Sbjct: 455 QLTDYDAFLSELNGQFGYAEYVSSAQEQLAALG 487 >C0UXG2.1 C0UXG2_9BACT Carbohydrate ABC transporter substrate-binding protein, CUT1 family Length = 567 Score = 160 bits (405), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 134/473 (28%), Positives = 220/473 (46%), Gaps = 42/473 (8%) Query: 45 PVDIEAAEISWGTSL---PADDFIKKALDEQVGVNLKLTLVGDQNDYENQMNVRAASGNL 101 PV IE T + P + F A+ + +++K++ + D + Y + RA + +L Sbjct: 86 PVSIEIYATVGSTEVGPPPKNWFWYDAVKKATNIDVKISWITDPSQYTPTLQTRAGANDL 145 Query: 102 PDLLMATSKAHLQKLAQSGSLLDLTPYLDKLPEYTKFVG-DEVLKKGILDSKQYAL-PKT 159 PDL T + LQ+L Q G + D +PYL +P + K +E+ K G D K Y L K Sbjct: 146 PDLF-TTDRNTLQQLVQQGLVADWSPYLKYMPNFVKDRDVNELAKYGTFDGKHYGLVTKN 204 Query: 160 GQSVSYTYWIRKDWLDNLQLQPPATVDELLEVAKAFTTNDPDKNGKNDTFGLTG-----T 214 IR+DWLD L L+PP T+DE LEV KAFT DPD NGK DT+G T Sbjct: 205 AYPYKGIVIIRQDWLDKLGLKPPKTIDEYLEVMKAFTEKDPDGNGKKDTYGYTAFIDDNG 264 Query: 215 NFQTFDPIFGAYGLTNIFDTSQL---FVRDGKVVSTFYQPEMKEALTTIKGFLDAGVVDP 271 FDP F A+G +QL F +D +VV + ++AL I ++ GV+DP Sbjct: 265 QISGFDPFFAAFG-------AQLDWKFEKD-QVVPMRISDQRRQALEFINKMVNEGVLDP 316 Query: 272 EIVSNKGTMAIDKAFQGKVGIINTEWAQIMKDDKVAVWKGANPKAEWIQLAPPKGPTGIA 331 + + +G +K GKVGI +W I + ANP + + + PP GP G + Sbjct: 317 DWKTQQGQDYANKWKTGKVGIFAGDWCAIFCRQGYQEFARANPNGKMVIIDPPVGPNGDS 376 Query: 332 NANSLNIGASSGLWVIPKKLESEPEKLNKVLELLNYVSTKEGNRLVQYGVENTHYTLEGD 391 A + + ++V+ KK ++ K + LL ++ T +G L +G E ++ + Sbjct: 377 AAGTYS--RLGNMYVMSKK-AADAGKGEAIARLLEWIDT-DGYYLTAFGQEGVNWKRDSQ 432 Query: 392 -TVKITDKGRSDASFTWLYQFTG---RPETDYLKSKFSN---------LAAYIDFEA--N 436 + K D L Q G + + L++++ N ++ + ++ Sbjct: 433 GHIVPGPKSYLDPENLVLTQLRGYAYKGTEEELRARYDNTYKAANGQTISVWSVYQRALK 492 Query: 437 LPRIQTLDGFVTSP-PGYNPADANRFIEEEMIKFVYGKAKIDSYSKFLETLEN 488 LP++ T + + P P AD R + E +KF G+ + ++ +++ Sbjct: 493 LPKLDTTNQAIIPPAPPEISADLTRTMAEAELKFATGQRPFSDWPNYVNQVKS 545 >B1DFA2.1 B1DFA2_9BACL Extracellular solute-binding protein family 1 Length = 525 Score = 153 bits (387), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 126/411 (30%), Positives = 190/411 (46%), Gaps = 40/411 (9%) Query: 3 KKNRVMAWTALAMTVALFSGWGGGSNTPKTAEGDASAKSAAGPVDIEAAEISW---GTSL 59 KK + A + + + +G TP A + + PV++ SW G Sbjct: 5 KKKWIAALGVVPLLAGVLAGCSDNGKTPAAASASQQPEKSKAPVELTV--FSWMFEGADA 62 Query: 60 PADDFIKKALDEQVGVNLKLTLVGDQNDYENQMNVRAASGNLPDLLMATSKA---HLQKL 116 P + I K L E++ + +K +V ND+E ++NV ASG +PD+ ++ A ++ Sbjct: 63 P-ESAIYKRLQEKLNIKIK-PIVASWNDWEEKLNVMIASGEMPDVFISNGIAKPVQYKQW 120 Query: 117 AQSGSLLDLTPYLDKLPEYTKFVGD-EVLKKGILDSKQYALPKTGQSVS-------YTYW 168 + G LL+L+ Y + P K + + EVL K + YALP +S S + Sbjct: 121 VKEGLLLNLSDYTAQYPNIGKSLANFEVLTKAT-EGNHYALPIYNESGSGKNSINGHNIL 179 Query: 169 IRKDWLDNLQLQPPATVDELLEVAKAFTTNDPDKNGKNDTFGLTGTNFQTFDPIFGAYGL 228 IR+DWLD L L+ P T+DE EVAKAFT +DPD NG DT+G T ++ ++ Y + Sbjct: 180 IRQDWLDKLNLKMPTTIDEFYEVAKAFTESDPDGNGIKDTYGYTSSS----GGVWWQYPI 235 Query: 229 TNIFDTSQLFVR---DGKVVSTFYQPEMKEALTTIKGFLDAGVVDPEIVSNKGTMAIDKA 285 N FD S + DGK P M++AL+ + + V+DP+ + N ++K Sbjct: 236 FNAFDASTDRFKKSADGKWEPEVISPGMEQALSFLNKMYNDKVIDPDFMINTDDQKVEKF 295 Query: 286 FQGKVGIINTEWAQIMKDD------KVAVWKGANPKAEWIQLAPPKGPTGIANANSLNIG 339 GKVG+I +D KV K N W+ KG G + Sbjct: 296 ITGKVGMIVNNLNATFYNDIYNKFKKVYEDKDPNTIINWV--GTLKGVNG-----EQRMD 348 Query: 340 ASSGLWV-IPKKLESEPEKLNKVLELLNYVSTKEGNRLVQYGVENTHYTLE 389 SS W EK K L LL+Y+ + EG L+ YG+EN HY +E Sbjct: 349 GSSNFWCETSINAGISDEKKQKALGLLDYLLSDEGQNLMLYGIENIHYKVE 399 >B3KF70.1 B3KF70_9BACI Putative uncharacterized protein Length = 133 Score = 149 bits (377), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 68/133 (51%), Positives = 98/133 (73%), Gaps = 1/133 (0%) Query: 377 VQYGVENTHYTLEGDTVKITDKGRSDASFTWLYQFTGRPETDYLKSKFSNLAAYIDFEAN 436 +Q+GV++TH+TLE K+TDK + +WLYQFTGRPE +YL++KF+ +A I+F + Sbjct: 1 MQFGVKDTHFTLEDGKPKMTDKA-GEVGHSWLYQFTGRPEMEYLQAKFAAQSAAIEFAND 59 Query: 437 LPRIQTLDGFVTSPPGYNPADANRFIEEEMIKFVYGKAKIDSYSKFLETLENTFSYKNYL 496 PRI+ L+GFV +P GYNPADANR++EEE+ KF+Y K + Y F++TLE + +YK + Sbjct: 60 QPRIEALNGFVDNPEGYNPADANRYMEEEIAKFIYDKRPLTEYDDFIKTLETSMNYKALM 119 Query: 497 DTAISQLNELGYG 509 D+A+ QL LGYG Sbjct: 120 DSAMDQLKALGYG 132 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,224,339,808 Number of extensions: 139410902 Number of successful extensions: 286289 Number of sequences better than 1.0e-05: 322 Number of HSP's gapped: 286004 Number of HSP's successfully gapped: 323 Length of query: 510 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 370 Effective length of database: 1,741,754,103 Effective search space: 644449018110 Effective search space used: 644449018110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)