BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0013.p (589 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAF1.1 B1DAF1_9BACL Integral membrane sensor signal transducti... 163 4e-38 B1DAE2.1 B1DAE2_9BACL Histidine kinase internal region 157 2e-36 B1DGY1.1 B1DGY1_9BACL Histidine kinase internal region 154 3e-35 A1HM13.1 A1HM13_9FIRM Integral membrane sensor signal transducti... 154 4e-35 B3KCS3.1 B3KCS3_9BACI Histidine kinase internal region 153 5e-35 >B1DAF1.1 B1DAF1_9BACL Integral membrane sensor signal transduction histidine kinase Length = 596 Score = 163 bits (413), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 95/290 (32%), Positives = 156/290 (53%), Gaps = 13/290 (4%) Query: 295 SFVLSLTLVQPIKKLLQLMKHVEVGDLRVRFSSKYTDEIGLLGRNFNRMLSNLSEMIDKM 354 S +L+ + + ++++ LM+ V G+L V F K DE+G+LG FNRML + E+I ++ Sbjct: 319 SVILAFMMTRSLRQMTDLMRTVRGGNLDVSFDVKQKDEVGILGTEFNRMLRRMKELISEI 378 Query: 355 YVVEMEKINAEMKQKDATMLAMQHQINPHFLYNTLEVINCQAILNDVPEISRMSKAMADF 414 YV++ K K+A + A+Q QINPHF+YNTLE I A +ND E++ M+ + Sbjct: 379 YVIQSRK-------KEADLEALQSQINPHFIYNTLETIRMTAEINDDEEVAEMTFTLGKL 431 Query: 415 FRYTVDSPRIEVELSVEIQHVRTYLEIQHERYPD-IEIDLDGLELFYAYPIIKLTVQPIV 473 RY+++ R VEL E+QH+ YL++ R+ + + D + +P++KLT QPIV Sbjct: 432 LRYSINRGRETVELREELQHLERYLQLLRYRFSNRFVVAFDIPQELMNFPVMKLTFQPIV 491 Query: 474 ENAFQYGFIGGRDYYLKVYAADISPDTYALIIEDNGEGMQTAAMEAMNALFAKESMELPQ 533 ENA +G G + +A++ I+D G A M+ M +++M + Sbjct: 492 ENAILHGMEGKPNEVTIRLSAELKEQLLIFSIKDEG-----AGMDGMTLDLLRQAMNGTR 546 Query: 534 GNASSRHGIGLLNVHHRIXXXXXXXXXXTLAESLTGGISVEILLPKEGKE 583 +ASS+ G+GL NV+ RI + G +V + LP++ E Sbjct: 547 ESASSKTGVGLRNVNERIKLHYGETYGLEIESKWGEGTTVNLKLPRKHGE 596 >B1DAE2.1 B1DAE2_9BACL Histidine kinase internal region Length = 627 Score = 157 bits (398), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 104/315 (33%), Positives = 165/315 (52%), Gaps = 36/315 (11%) Query: 295 SFVLSLTLVQPIKKLLQLMKHVEVGDLRVRFSSKYTDEIGLLGRNFNRMLSNLSEMIDKM 354 S+ LS ++ +PI +L M+ VE G++ +R + DEIGLLGR+FN+MLS ++ +I ++ Sbjct: 319 SYYLSHSISRPISQLASFMRKVEEGNMSIRIPEEREDEIGLLGRSFNKMLSQMTRLISQV 378 Query: 355 YVVEMEKINAEMKQKDATMLAMQHQINPHFLYNTLEVINCQAILNDVPEISRMSKAMADF 414 + + K AE++ ++Q I PHFLYNTL+ I A + E + M +A++ Sbjct: 379 WAEQRLKREAELR-------SLQAHIQPHFLYNTLDTIQWLARKDGAKEATEMVEALSKL 431 Query: 415 FRYTVDSPRIEVELSVEIQHVRTYLEIQHERYPD-----IEIDLDGLELFYAYPIIKLTV 469 FR + + + L+ EI+H+R+YL+IQ RY D IE+D +LF ++KL + Sbjct: 432 FRIGLSKGQEMIPLTDEIEHIRSYLKIQQTRYKDKLNYNIEVDGSCDDLF----VLKLIL 487 Query: 470 QPIVENAFQYGFIGGRDYYLKVYAADISPDTYALIIEDNGEGMQTAAMEAMNALF----- 524 QP+VENA +G R + A IS + L+I+D+G GM A +EA+ A+ Sbjct: 488 QPLVENAIYHGIKERRGPGMITIRAIISEEILELVIQDDGAGMTEARLEALRAVLQAVPS 547 Query: 525 ---------AKESMELPQGNASSRHGIGLLNVHHRIXXXXXXXXXXTLAESLTGGISVEI 575 A E + QG A+S GL NV RI +A G +V I Sbjct: 548 VHEANAAQRAAEERQQGQGQAAS---YGLRNVQERIRLSFGETYGIAIASKEKAGTTVII 604 Query: 576 LLP---KEGKENEDS 587 P ++G++ D+ Sbjct: 605 KHPIIHRKGQDTHDA 619 >B1DGY1.1 B1DGY1_9BACL Histidine kinase internal region Length = 597 Score = 154 bits (388), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 96/287 (33%), Positives = 147/287 (51%), Gaps = 13/287 (4%) Query: 295 SFVLSLTLVQPIKKLLQLMKHVEVGDLRVRFSSKYTDEIGLLGRNFNRMLSNLSEMIDKM 354 S +++ +PI +L Q MK VE+ + V +DEIG L R FN MLS + E+I Sbjct: 317 SVLIAYITTRPIIRLTQSMKAVEIQNFEVDIREPRSDEIGTLERRFNSMLSRIKELIQTE 376 Query: 355 YVVEMEKINAEMKQKDATMLAMQHQINPHFLYNTLEVINCQAILNDVPEISRMSKAMADF 414 Y +EK A++K AMQ QINPHFL+N L+ I A+ +VPEI+ +A++D Sbjct: 377 YKATIEKRTAQVK-------AMQAQINPHFLHNALQAIGGIALSRNVPEINDHVRAISDL 429 Query: 415 FRYTVDSPRIEVELSVEIQHVRTYLEIQHERYPD-IEIDLDGLELFYAYPIIKLTVQPIV 473 FRYT+ V ++ E++H R YL+IQ R+ IEI +D E I K ++QPIV Sbjct: 430 FRYTIRMKSDLVTIADELEHARNYLQIQKLRFQSMIEIRIDADEECLGCRIPKFSLQPIV 489 Query: 474 ENAFQYGFIGGRDYYLKVYAADISPDTYALIIEDNGEGMQTAAMEAMNALFAKESMELPQ 533 EN F +G G D + + D + I DNG G+ A + + + + P Sbjct: 490 ENCFVHGLEGKMDAWTISIRVEKVLDEIEIDIADNGMGIDEARLAEI-----RRQLSQPA 544 Query: 534 GNASSRHGIGLLNVHHRIXXXXXXXXXXTLAESLTGGISVEILLPKE 580 + G+G+ NV+ RI ++ G +V++L+P + Sbjct: 545 DGQENGEGMGISNVNARIKLLFGDEYGLFISSEWNAGTTVKLLIPAQ 591 >A1HM13.1 A1HM13_9FIRM Integral membrane sensor signal transduction histidine kinase Length = 612 Score = 154 bits (388), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 83/256 (32%), Positives = 144/256 (56%), Gaps = 8/256 (3%) Query: 298 LSLTLVQPIKKLLQLMKHVEVGDLRVRFSSKYTDEIGLLGRNFNRMLSNLSEMIDKMYVV 357 + +L++PI++L +MK VEVG+ VR + DEIG L R FN+M+ L+ +++++Y Sbjct: 325 FAASLIRPIRRLQHVMKSVEVGNFNVRAAILSQDEIGELARGFNKMVERLAGLMEEVYFT 384 Query: 358 EMEKINAEMKQKDATMLAMQHQINPHFLYNTLEVINCQAILNDVPEISRMSKAMADFFRY 417 ++ + A + QK+ + A+Q Q+NPHFL NTLE + A+ D+ +I+ ++ ++ RY Sbjct: 385 KLRETEARLGQKEMELKALQSQLNPHFLCNTLETVRGMALARDMEDIATIAASLGQLLRY 444 Query: 418 TVDSPRIEVELSVEIQHVRTYLEIQHERYPD-IEIDLDGLELFYAYPIIKLTVQPIVENA 476 + S + V L E++ + YL+IQ R+ + D E + I+K ++QP++EN Sbjct: 445 NLRSHALLVPLREEVKFCQLYLKIQQFRFGNRFTYKFDIPEWAWDLQIVKFSLQPLLENC 504 Query: 477 FQYGF-IGGRDYYLKVYAADISPDTYALIIEDNGEGMQTAAMEAMNALFAKESMELPQGN 535 F +GF G + V A D Y + + DNG GM+ A +E + A ++ + Sbjct: 505 FAHGFQYGESPLAITVSARREGDDAYIVEVADNGAGMEPAVLERIRADLTQKDV------ 558 Query: 536 ASSRHGIGLLNVHHRI 551 + IG++NVH RI Sbjct: 559 TAGGQSIGIVNVHRRI 574 >B3KCS3.1 B3KCS3_9BACI Histidine kinase internal region Length = 623 Score = 153 bits (386), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 99/302 (32%), Positives = 161/302 (53%), Gaps = 14/302 (4%) Query: 301 TLVQPIKKLLQLMKHVEVGD-LRVRFSSKYTDEIGLLGRNFNRMLSNLSEMIDKMYVVEM 359 ++V PI+ L M+ E G+ + DEIG L ++N M+S LS+MI+++Y E+ Sbjct: 317 SIVNPIRNLKNGMRQTEKGNWTHITDVKPREDEIGGLIHSYNLMVSRLSDMIEQVYDTEL 376 Query: 360 EKINAEMKQKD-------ATMLAMQHQINPHFLYNTLEVINCQAILNDVPEISRMSKAMA 412 +++ +D A A+Q QINPHFLYNTLE INC AI+ D EIS M +AMA Sbjct: 377 HNQKTQLELQDIQLERQRAEFQALQLQINPHFLYNTLETINCYAIVQDSDEISDMVEAMA 436 Query: 413 DFFRYTVDSPRIEVELSVEIQHVRTYLEI-QHERYPDIEIDLDGLELFYAYPIIKLTVQP 471 RY++ + E+ ++ E+ HVR Y+ I +H D EI++ +++L++QP Sbjct: 437 FMLRYSIQTNLEEITIANELNHVRHYMMILKHRIGRDFEIEVATPPELLLEKMVRLSLQP 496 Query: 472 IVENAFQYGFIGGRD--YYLKVYAADISPDTYALIIEDNGEGMQTAAMEAMNALFAKESM 529 IVEN FQ+ F G + +Y+++ + D +I+EDNG G+ + + + + Sbjct: 497 IVENIFQHAFPEGIEAWHYIRI-DTRVDGDNLLVIVEDNGAGIADHKLHKLRKRLEENRL 555 Query: 530 --ELPQGNASSRHGIGLLNVHHRIXXXXXXXXXXTLAESLTGGISVEILLPKEGKENEDS 587 + A R GIG++NVH RI T+ S + G S+ + +P+ ++ E Sbjct: 556 VDDEEGKRARRRGGIGIVNVHRRIQMVYGEAYGITVHSSPSQGTSIVMTMPRMERKCEQE 615 Query: 588 GR 589 R Sbjct: 616 NR 617 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,055,396,624 Number of extensions: 119636119 Number of successful extensions: 331572 Number of sequences better than 1.0e-05: 1582 Number of HSP's gapped: 329404 Number of HSP's successfully gapped: 1583 Length of query: 589 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 447 Effective length of database: 1,722,912,073 Effective search space: 770141696631 Effective search space used: 770141696631 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)