BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0011.p (455 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5ESH8.1 C5ESH8_9FIRM Extracellular solute-binding protein 522 e-146 C1XUW3.1 C1XUW3_9DEIN Carbohydrate ABC transporter substrate-bin... 205 8e-51 C5CS15.1 C5CS15_VARPD Extracellular solute-binding protein family 1 197 2e-48 Q1J2N4.1 Q1J2N4_DEIGD ABC-type sugar transport system, periplasm... 188 1e-45 Q9ZC22.1 Q9ZC22_YERPE Similar to AF039956_5 Sinorhizobium fredii 182 6e-44 >C5ESH8.1 C5ESH8_9FIRM Extracellular solute-binding protein Length = 455 Score = 522 bits (1344), Expect = e-146, Method: Compositional matrix adjust. Identities = 247/436 (56%), Positives = 321/436 (73%), Gaps = 4/436 (0%) Query: 20 KNAENTKGEAAQSSAGGSTD--TLEAWGKTIKEKYSGQEIKLAFASHPSTESFQKMTGDF 77 K+ + AAQ S S D T+E WGK +KE+Y G I ++ ASHPSTE+FQ M G+F Sbjct: 21 KSGTEAQDTAAQESTEASADASTMEGWGKLMKEQYDGTRIVVSMASHPSTEAFQTMAGEF 80 Query: 78 EKLTGIKVKWEVMEETYLKNKQLLDFTGKTGSYDVLMVDGFWMSEYGAKKVVEPLDTFIG 137 LTGI+V+W+V+E+TYLKNKQLLDF G SYDV MVD FW +EYGAK VV+PLD ++ Sbjct: 81 TDLTGIQVEWDVVEQTYLKNKQLLDFQG-AHSYDVFMVDSFWNAEYGAKNVVQPLDDYMN 139 Query: 138 NKDLTPAWFDYEDIVPAYRNGISKYNDKIYGIPTAGETRFVAYRKDLFEKYDKQPPKTMA 197 + TP W+D D++PAY + + +Y + GIP AGETRF+AYR DLFEKY K+PPKTM Sbjct: 140 DPQKTPEWYDKADLIPAYCD-LGQYKGETIGIPIAGETRFLAYRTDLFEKYGKEPPKTMD 198 Query: 198 EFLELAKFFNGKESGLYGVSMRAQRGIHAASGLMTVMYNFGGGFMDQKTGAVTMTDPKTV 257 EFLELA+FFNGKE GLYGV++RAQRGIH ASG +T MY F G MDQKT + DP V Sbjct: 199 EFLELARFFNGKEDGLYGVALRAQRGIHFASGWLTTMYAFCDGMMDQKTLEPKINDPGVV 258 Query: 258 EAMQFYVDLLKNAPKDVASYTHEEALSAFTSGKAAMWLDATALATRILDPSSSQVYDKVG 317 E++Q+Y+D+L+ AP DV++YTHEEAL AF SGK AMWLDATA+A+ I DP S+VYDKV Sbjct: 259 ESLQWYLDMLECAPPDVSTYTHEEALGAFMSGKTAMWLDATAIASAITDPQKSKVYDKVA 318 Query: 318 FVPTPEGPAGKASALAGWNMSLSALSKNKEAAWAFIVYMNSKSNAKTYVQGGGVPVRTSI 377 F P+GPAG+++ALA WN+S+ +KN +AAWAF++YM S+ + YV+ GG R S+ Sbjct: 319 FAAPPDGPAGESAALADWNISIPNGAKNPDAAWAFMMYMTSREKSLEYVKNGGAATRYSV 378 Query: 378 YKDEELVAADPSYPVQLEALNDANVLVEKGISWIPPHEKLGQILDRVGYYVSAAMTGEMT 437 Y++EEL+ +PSYP QL AL AN LV++G+ W PPHE++ Q+LD +G Y S A GEMT Sbjct: 379 YENEELIKENPSYPAQLAALEKANGLVKRGLQWTPPHEQINQMLDIMGSYASRAQAGEMT 438 Query: 438 VQEAAQKSQTELEDIV 453 QEA K+Q ++ D++ Sbjct: 439 AQEACDKAQADVLDLL 454 >C1XUW3.1 C1XUW3_9DEIN Carbohydrate ABC transporter substrate-binding protein, CUT1 family Length = 449 Score = 205 bits (521), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 121/409 (29%), Positives = 215/409 (52%), Gaps = 11/409 (2%) Query: 52 YSGQEIKLAFASHPSTESFQKMTGDFEKLTGIKVKWEVMEETYLKNKQLLDFTGKTGSYD 111 Y+G IK F P ++ K+ +FEK+TGIKV EV+ + K +LD T + G YD Sbjct: 35 YAGTSIKAIFLDRPGYKAAAKLLPEFEKITGIKVNTEVIPYENTREKIVLDMTARGGEYD 94 Query: 112 VLMVDGFWMSEYGAKKVVEPLDTFIGNKDLTPAWFDYEDIVPAYRNGISKYNDKIYGIPT 171 V++ D W+ E+ + + PL+ F + L + + P + ++ KIYG+P Sbjct: 95 VVLTDVVWIGEFASNGWLVPLEKFYNDPKLADPALNLKGFFPILLDSFGTWDKKIYGLPF 154 Query: 172 AGETRFVAYRKDLFEKYD-KQPPKTMAEFLEL-AKFFNGKESGLYGVSMRAQRG-IHAAS 228 + Y K + +K +PP T E L++ A ++G+Y +++++RG +A Sbjct: 155 DNYAGLLFYNKCMLQKAGFSKPPATWEELLKVYAPKLTNPKAGVYAFALQSRRGETQSAD 214 Query: 229 GLMTVMYNFGGGFMDQKTGAVTMTDPKTVEAMQFYVDLLKNAPKDVASYTHEEALSAFTS 288 M +++ FGG +D KT + P ++ +QF DL+K P + Y H EA++A Sbjct: 215 SFMRMLWPFGGSLIDPKTFEPNLLSPASLRGLQFRQDLMKYMPPGIVDYDHNEAVNALAQ 274 Query: 289 GKAAMWLDATALATRILDPSSSQVYDKVGFVPTPEGPAGKASALAGWNMSLSALS--KNK 346 GK AM + +A T + DP SS++ + +G P GPAG AL G++ ++A S + + Sbjct: 275 GKVAMITEWSAFYTTLADPKSSKLGNCLGVATEPAGPAGLKGALGGFSYGVNAFSPPERQ 334 Query: 347 EAAWAFIVYMNSKSNAKTYVQGGGVPVRTSIYKDEELVAADPSYPVQLEALNDANVLVEK 406 AA+ FI ++ S++ AK YV+ GGV R S+YKD + A P ++A N + Sbjct: 335 AAAYLFIQWITSEAKAKDYVRAGGVSARMSVYKDPAIQKAFPFTVPMVKAWEQGNPIFRP 394 Query: 407 GISWIPPHEKLGQILDRVGYYVSAAMTGEMTVQEAAQKSQTELEDIVGK 455 P ++ +++ ++G + M G+++V++ A + ++++I+ K Sbjct: 395 R---FPEWPEISEVIAQIG---TEMMLGKVSVKDGAAQINAKVKEILTK 437 >C5CS15.1 C5CS15_VARPD Extracellular solute-binding protein family 1 Length = 443 Score = 197 bits (500), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 123/402 (30%), Positives = 202/402 (50%), Gaps = 20/402 (4%) Query: 51 KYSGQEIKLAFASHPSTESFQKMTGDFEKLTGIKVKWEVMEETYLKNKQLLDFTGKTGSY 110 KY+GQ + L SHP ++ K+ +F + TGIKV+ + + LK+KQLL+ G Y Sbjct: 32 KYAGQTVTLNVPSHPHYDAMAKILPEFTRETGIKVEIDKLAMARLKDKQLLEMAKPQGDY 91 Query: 111 DVLMVDGFWMSEYGAKKVVEPLDTFIGNKDLTPAWFDYEDIVPAYRNGISKYND------ 164 D++ W EY AK +V PL+ ++ + L +D +DI+P Y + Sbjct: 92 DLVSYVVMWKGEYAAKNLVRPLEPYLSDAALADPAYDIKDIIPIYLENLGLVGGPKGYLP 151 Query: 165 ----KIYGIPTAGETRFVAYRKDLFEKYDKQPPKTMAEFLELAKFFNGKESGLYGVSMRA 220 K+YG+P ET +AYR+D+F K++ QPP+ +F +L + KE G+ ++ R Sbjct: 152 GAGAKLYGLPYGAETSVLAYRRDIFAKHNLQPPENYEQFGKLLRLIKDKE-GMGALASRG 210 Query: 221 QRGIHAASGLMTVMYNFGGGFMDQKTGAVTMTDPKTVEAMQFYVDLLKNAPKDVASYTHE 280 Q G + + GG D + D V A++F +++ P +A Y Sbjct: 211 QAGHQCVHAWLLHLNPMGGAIFDARW-EPRFNDSVGVAALKFLQEVVATGPAGIAGYGQG 269 Query: 281 EALSAFTSGKAAMWLDATALATRILDPSSSQVYDKVGFVPTPEGPAGKASALAGWNMSLS 340 E+ +AF G+A+M+LD+T LA + DP+ S+V KV + P G ++S G +++ Sbjct: 270 ESNTAFLQGQASMYLDSTLLAGLVNDPAKSRVAGKVSWALHPAG-TRRSSQSGGLGLAIP 328 Query: 341 ALSKNKEAAWAFIVYMNSKSNAKTYVQGGGVPVRTSIYKDEELVAADPSYPVQLEALNDA 400 +KN +A + + ++ SK+ K + GGVP+R S D +LV P Y V EAL + Sbjct: 329 KNAKNADAGFLLMQWLTSKAQDKAVARAGGVPIRNSTMADADLVRQYPEYIVFREALKYS 388 Query: 401 NVLVEKGISWIPPHEKLGQI-LDRVGYYVSAAMTGEMTVQEA 441 N W P +I + +G +S A+ G+ T + A Sbjct: 389 NP------DWRPIIAAWDKINVQALGVGISEALIGKKTAEAA 424 >Q1J2N4.1 Q1J2N4_DEIGD ABC-type sugar transport system, periplasmic component Length = 428 Score = 188 bits (477), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 127/404 (31%), Positives = 199/404 (49%), Gaps = 25/404 (6%) Query: 54 GQEIKLAFASHPSTESFQKMTGDFEKLTGIKVKWEVMEETYLKNKQLLDFTGKTGSYDVL 113 G +++ HP T + Q +FEKLTG+K+ E E + K L++ + TG ++ Sbjct: 31 GTTLRVLLNQHPWTTAMQPAFPEFEKLTGMKLAVETYPEAQFRQKVLVELS--TGGQNL- 87 Query: 114 MVDGFWMSE------YGAKKVVEPLDTFIGNKDLTPAWFDYEDIVPAYRNGISKYNDKIY 167 D F +S Y +E + T+I NK+LT + + D P+ ++YN + Sbjct: 88 --DAFMLSPGQEGLLYARSGWIEDMKTYINNKNLTAGNWGFSDFYPSVVRS-TEYNGIMT 144 Query: 168 GIPTAGETRFVAYRKDLFEKYDKQPPKTMAEFLELAKFFNGKESGLYGVSMRAQRGIHAA 227 G+P ET + YRKDLF KY PKTMA+ AK +GK+ G++G+++R +G A Sbjct: 145 GVPIQTETPMLFYRKDLFTKYKIPVPKTMAQLEAAAKALHGKD-GVFGIALRG-KGAAAT 202 Query: 228 SGLMTVMYNFGGGFMDQKTGAVTMTDPKTVEAMQFYVDLLKN-APKDVASYTHEEALSAF 286 S M+++G +++ K G TDPK V+AM Y LL+ P + + E + F Sbjct: 203 SQFSPYMFSYGSTWLN-KDGQANFTDPKFVQAMTMYTGLLRKYGPPAAVTMSWPEVTNLF 261 Query: 287 TSGKAAMWLDATALATRILDPSSSQVYDKVGFVPTPEGPAGKASALAGWNMSLSALSKNK 346 GK AM+ DA+ + + DP SS V KVG+ P P GPAG+ + W +S+ SKNK Sbjct: 262 AQGKVAMFTDASLFRSIVDDPKSSTVAGKVGYAPFPAGPAGRKPYVTTWALSIPKGSKNK 321 Query: 347 EAAWAFIVYMNSKSNAKTYVQGGGVPVRTSIYKDEEL--VAADPSYP-VQLEALNDANVL 403 AW F + ++ N + VR S++ D +P + L L AN L Sbjct: 322 PCAWLFTQWATNRQNQLRVLLQDVPAVRRSVWNDPAFKKQETNPEWTQAHLSQLASANPL 381 Query: 404 VEKGISWIPPHEKLGQILDRVGYYVSAAMTGEMTVQEAAQKSQT 447 W PP ++G++ D +G + + G T+ + QT Sbjct: 382 ------WNPPVSQVGEVRDALGQAIVGILQGGNTLDLLKRAEQT 419 >Q9ZC22.1 Q9ZC22_YERPE Similar to AF039956_5 Sinorhizobium fredii Length = 422 Score = 182 bits (462), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 128/405 (31%), Positives = 205/405 (50%), Gaps = 20/405 (4%) Query: 53 SGQE-IKLAFASHPSTESFQKMTGDFEKLTGIKVKWEVMEETYLKNKQLLDFTGKTGSYD 111 S QE +++ H +++S + + +FEK TGIKV+ E++ + L +K LL F+ +G YD Sbjct: 22 SAQETLRVLLEGHSTSDSIKALLPEFEKQTGIKVQAEIVPYSDLTSKALLAFSSHSGRYD 81 Query: 112 VLMVDGFWMSEYGAKKVVEPLDTFIGNKDLTPAWFDYEDIVPAYRNGISKYNDKIYGIPT 171 V+M D Y + + P+D ++ + A++D D V +Y + + +Y D YG+P Sbjct: 82 VVMDDWVHAVGYASAGYITPVDQWM---ESDTAFYDGADFVKSYADTL-RYKDGYYGLPV 137 Query: 172 AGETRFVAYRKDLFEKYDKQPPKTMAEFLELAKFFNGKESG-LYGVSMRAQRGIHAASGL 230 GE+ F+ YRKDLFE+Y PKT E AK K G + G+++R +GI Sbjct: 138 YGESTFLMYRKDLFEQYGIAVPKTFDELTAAAKTIKEKTEGKVAGITLRGAQGIQNTFAW 197 Query: 231 MTVMYNFGGGFMDQKTGAVTMTDPKTVEAMQFYVDLLKN-APKDVASYTHEEALSAFTSG 289 + ++ +GG ++D G +T P+ VEA + +V++LKN P A++ +E F G Sbjct: 198 ASFLWGYGGQWIDDN-GKSAITSPQAVEATKSFVNILKNYGPIGAANFGWQENRLVFQQG 256 Query: 290 KAAMWLDATALATRILDPSSSQVYDKVGFVPTPEGPA---GKASALAGWNMSLSALSKNK 346 KAAM +D+T DP S V KVG+ P P P G + AL + +S+ SK + Sbjct: 257 KAAMTIDSTVNGGFNEDPKESTVVGKVGYAPVPVQPGDHPGNSGALQVHGLYISSDSKKQ 316 Query: 347 EAAWAFIVYMNSKSNAKTYVQ---GGGVPVRTSIYKDEELVAADPSYPVQLEALNDANVL 403 +AAW FI + K V+ GV ++I D L AL + N Sbjct: 317 DAAWKFISWATDKQTQMKSVELNPNAGVSSLSAINSDAFTKRYGAFKDGMLAALQNGNA- 375 Query: 404 VEKGISWIPPHEKLGQILDRVGYYVSAAMTGEMTVQEAAQKSQTE 448 ++P + QI++ G +S A+ G TV+ A Q++ T Sbjct: 376 -----KYLPTIPQSTQIINITGIALSEALAGTQTVENALQQANTR 415 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.130 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,644,503,475 Number of extensions: 113537092 Number of successful extensions: 247557 Number of sequences better than 1.0e-05: 1405 Number of HSP's gapped: 245739 Number of HSP's successfully gapped: 1408 Length of query: 455 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 316 Effective length of database: 1,751,175,118 Effective search space: 553371337288 Effective search space used: 553371337288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)