BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0010.p (294 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5ESH6.1 C5ESH6_9FIRM Transport protein 322 2e-86 Q986P7.1 Q986P7_RHILO ABC transporter, permease protein 187 1e-45 C5CIF8.1 C5CIF8_9THEM Binding-protein-dependent transport system... 187 1e-45 A6WVT9.1 A6WVT9_OCHA4 Binding-protein-dependent transport system... 186 3e-45 B6A213.1 B6A213_RHILW Binding-protein-dependent transport system... 184 6e-45 >C5ESH6.1 C5ESH6_9FIRM Transport protein Length = 294 Score = 322 bits (826), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 163/293 (55%), Positives = 216/293 (73%), Gaps = 4/293 (1%) Query: 1 MIRTYFQKNPHLAYIMPAMIILGTLTFIPTLFLYVLSVSDYELGYP--AFQFVGLDNFIR 58 M++ Y +K P++ YI PAM I+ LT PT+FLY++S++DY+LG+ + V DN+IR Sbjct: 1 MMKRYIKKKPYIPYIAPAMAIMTVLTVFPTVFLYLISITDYQLGWDLGRVRLVWFDNYIR 60 Query: 59 LFSGGDAEFWYSVTISLGFMGIVTVIELLLGFWLAVLL-DREFKLKFLVFACMIVPIAMT 117 LFSG D +FW +V ISL FM I T IE++LGF + LL D EFKLK ++FA +I+PI MT Sbjct: 61 LFSGRDPDFWNAVYISLKFMVITTGIEMVLGFMIGKLLCDSEFKLKPVIFAILIIPIVMT 120 Query: 118 PSITGQVWKLMMNAESGVLNYLLHFIG-GKVIWLSGENAFWSTVLVDVWQNTPFVALIIY 176 PSI G +WKLM+N+E G++NY L+ +G V WL +F S VL DVWQ TPFV+LI Y Sbjct: 121 PSIAGNIWKLMLNSEYGIVNYFLNLVGISNVTWLDANLSFTSVVLADVWQWTPFVSLISY 180 Query: 177 AGIRSLPTDPYEAAAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQ 236 AG+ SLP D YEAA IDG + Q+FR ITLP L P+I L +IFR IDSLK +D+P+ LTQ Sbjct: 181 AGLCSLPADQYEAARIDGGNAFQLFRYITLPSLRPLIFLTLIFRTIDSLKIYDMPFVLTQ 240 Query: 237 GGPGSATEFLSLHVYRLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKG 289 GGPG++TEFLSLH+YRL AQ G +GR+AA ++IL++++T +S LLI RKG Sbjct: 241 GGPGNSTEFLSLHIYRLANAQNGLIGRAAANAIILMVISTMVSKLLIHYQRKG 293 >Q986P7.1 Q986P7_RHILO ABC transporter, permease protein Length = 307 Score = 187 bits (474), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 116/281 (41%), Positives = 168/281 (59%), Gaps = 9/281 (3%) Query: 16 MPAMII-LGTLTFIPTLFLYVLSVSDYELGYP-AFQFVGLDNFIRLFSGGDAEFWYSVTI 73 +PA+++ +G L IP L V S Y L P A QF DN+I F+ D +FW ++ + Sbjct: 27 LPALLVCIGIL--IPFLTSVVYSFQRYRLSQPWARQFNWGDNYISFFT--DPKFWNTLKV 82 Query: 74 SLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMTPSITGQVWKLMMNAES 133 SL + G V+ELLLG +A+LL + L + +++P+ P++ +WKLM N Sbjct: 83 SLLYAGTTVVLELLLGLAIAMLLQKRSTLNNFISIMLLMPLMTAPALAALMWKLMTNPGF 142 Query: 134 GVLNYLLHFIG-GKVIWLSG-ENAFWSTVLVDVWQNTPFVALIIYAGIRSLPTDPYEAAA 191 GVL+YL IG W S A ++ VLVD+W TPF+ +++ AG+RSLPT P+EAAA Sbjct: 143 GVLSYLASLIGLQDFRWASSPSTALFTVVLVDIWVYTPFIMILLLAGLRSLPTQPFEAAA 202 Query: 192 IDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEFLSLHVY 251 +DG R +F ITLP+L P IL A +FR +DS++ FDI Y++TQGGPG + Y Sbjct: 203 LDGVPRSFVFFRITLPMLTPYILTATLFRLLDSIQQFDIIYAMTQGGPGDTLTVFQVEAY 262 Query: 252 RLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKGVEN 292 L F Q+ VGRSAA+ +IL +T +S + IK + + E Sbjct: 263 -LNFFQSTNVGRSAALMIILWAITYFLSNIFIKNWLRLRER 302 >C5CIF8.1 C5CIF8_9THEM Binding-protein-dependent transport systems inner membrane component Length = 297 Score = 187 bits (474), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 114/292 (39%), Positives = 184/292 (63%), Gaps = 12/292 (4%) Query: 1 MIRTYFQKNPHLA---YIMPAMIILGTLTFIPTLFLYVLSVSDYELGYPAFQFVGLDNFI 57 MI Y KN L + P++I+LG + +P ++ +++S SDY P F FVG N++ Sbjct: 1 MISKY-MKNEKLTGWIFATPSLIVLGAVIAVPLIYSFIVSFSDYTFVSPFFNFVGFQNYL 59 Query: 58 RLFSGGDAEFWYSVTISLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMT 117 F D+ FW S+ ++L F+ +V +E +GF +A++L+RE +LK L + + +P+ M+ Sbjct: 60 AAFQ--DSYFWNSLKVTLKFVVLVVSMEFAIGFVIALMLNREIRLKKLYYTILTLPMVMS 117 Query: 118 PSITGQVWKLMMNAESGVLNYLLHFIGGK-VIWLSGE-NAFWSTVLVDVWQNTPFVALII 175 P G +WK++++ + GV+NY + +G K V WL+ NAFW+ ++VD+WQ F+ L++ Sbjct: 118 PVAVGLIWKMLLHPDLGVINYFITSLGLKPVNWLANSTNAFWTLIMVDIWQQVSFMILLL 177 Query: 176 YAGIRSLPTDPYEAAAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLT 235 AG+ SLP +PYEAA IDGA+ Q F ITLPL+ P+I A+ R I + +T+D+ Y LT Sbjct: 178 LAGLVSLPKEPYEAATIDGANETQKFLYITLPLMKPVIAAAITIRIILAFRTYDLVYVLT 237 Query: 236 QGGPGSATEFLSLHVYRLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYR 287 +GGPG TE LS +YR F + ++A S ILL++ + +++I A+R Sbjct: 238 KGGPGVKTEVLSYFIYRKTFMGLN-LSEASATSYILLLV---VMVIVILAFR 285 >A6WVT9.1 A6WVT9_OCHA4 Binding-protein-dependent transport systems inner membrane component Length = 312 Score = 186 bits (471), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 104/288 (36%), Positives = 176/288 (61%), Gaps = 6/288 (2%) Query: 8 KNPHLAYIMPAMIILGTLTFIPTLFLYVLSVSDYELGYPAFQFVGLDNFIRLFSGGDAEF 67 + + +++PA+I +G + P +F +S + ++LG FVG DN++RL D F Sbjct: 29 RPSYWPFVLPALITVGAVIVFPWVFTLWMSANQWQLGGEQ-SFVGFDNYLRL--AADMRF 85 Query: 68 WYSVTISLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMTPSITGQVWKL 127 W S+ +L + + V ++LG A++ D + ++ L+ ++P+ TP VW + Sbjct: 86 WESMWHTLVYTVLSVVAPMILGTIAALVFDSKLPMRGLLRGIFVMPMMATPVAVALVWTM 145 Query: 128 MMNAESGVLNYLLHFIG-GKVIWLSGENA-FWSTVLVDVWQNTPFVALIIYAGIRSLPTD 185 M + + GVLNYLL IG W+ ++ S VLV+ WQ TP V LI+ G+ S+P D Sbjct: 146 MYHPQLGVLNYLLSLIGIAPQEWIFNQSTVIPSLVLVETWQWTPLVMLIVLGGLASMPRD 205 Query: 186 PYEAAAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEF 245 P+E+A IDGA+ Q FR ITLP++ P I++AVI R+ID+LK+FDI Y++TQGGPG+A+E Sbjct: 206 PFESAEIDGANGWQKFRYITLPMILPFIMVAVIIRSIDALKSFDIIYAMTQGGPGTASET 265 Query: 246 LSLHVYRLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKGVENK 293 +++++Y + F+ +G +A++V+ I+ +SM+L+ ++ N Sbjct: 266 INIYLYNVAFSYYD-IGYGSAIAVVFFIVIIAMSMILLALRQRTKWNS 312 >B6A213.1 B6A213_RHILW Binding-protein-dependent transport systems inner membrane component Length = 313 Score = 184 bits (468), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 105/283 (37%), Positives = 175/283 (61%), Gaps = 6/283 (2%) Query: 14 YIMPAMIILGTLTFIPTLFLYVLSVSDYELGYPAFQFVGLDNFIRLFSGGDAEFWYSVTI 73 +++PA+I++ + P +F +SV+ + LG F GLDN+ RL D FW S+ Sbjct: 32 FVIPALIVIAAVIVFPWVFTLWMSVNSWTLGQSQV-FAGLDNYARL--AVDMRFWESLWH 88 Query: 74 SLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMTPSITGQVWKLMMNAES 133 ++ + + V L LG A++ D +F L+ L+ ++P+ TP VW +M + + Sbjct: 89 TVLYTTLSVVAPLFLGTLAALIFDTQFPLRGLLRGIFVMPMMATPVAIALVWTMMFHPQL 148 Query: 134 GVLNYLLHFIG-GKVIWLSGENAFW-STVLVDVWQNTPFVALIIYAGIRSLPTDPYEAAA 191 GVLNYLL FIG G W+ +N+ S VLV+ WQ TP + LI+ G+ ++P +PYE+A Sbjct: 149 GVLNYLLSFIGIGPQEWIYNQNSVIPSLVLVETWQWTPLIMLIVLGGLAAVPREPYESAE 208 Query: 192 IDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEFLSLHVY 251 IDGA+ Q FR +TLP++ P +++AVI R+ID++K+FDI Y++TQGGPG+A+E +++++Y Sbjct: 209 IDGANVWQKFRYLTLPMIAPFLMIAVIIRSIDAVKSFDIIYAMTQGGPGTASETINIYLY 268 Query: 252 RLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKGVENKG 294 FA +G +A++V+ IL +S +L+ ++ + G Sbjct: 269 NTAFAYYD-IGYGSAMAVVFFILIVLLSFVLLMLRQRANWSDG 310 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.144 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,769,227,892 Number of extensions: 73812173 Number of successful extensions: 261174 Number of sequences better than 1.0e-05: 6821 Number of HSP's gapped: 255698 Number of HSP's successfully gapped: 6826 Length of query: 294 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 160 Effective length of database: 1,798,280,193 Effective search space: 287724830880 Effective search space used: 287724830880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)