BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0005.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4ICG9.1 C4ICG9_CLOBU Aminoglycoside N 346 1e-93 B1QUL3.1 B1QUL3_CLOBU Aminoglycoside N3'-acetyltransferase 346 1e-93 A6M2W6.1 A6M2W6_CLOB8 Aminoglycoside N(3')-acetyltransferase EC=... 321 4e-86 C4GBE1.1 C4GBE1_9FIRM Putative uncharacterized protein 291 4e-77 A6CL21.1 A6CL21_9BACI Aminoglycoside N3'-acetyltransferase 225 4e-57 >C4ICG9.1 C4ICG9_CLOBU Aminoglycoside N Length = 267 Score = 346 bits (888), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 158/266 (59%), Positives = 205/266 (77%) Query: 8 LTRSNETRMIFTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGE 67 + + + R I KEDLI+ F G+ +GQ I+VHTSL GF+ GGA+ I ALLE V + Sbjct: 1 MKNNEKERKIILKEDLIEGFKKLGVKEGQSIMVHTSLKSMGFVCGGAQIVIEALLESVKD 60 Query: 68 EGTLMMPSQTWKNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWP 127 +GT+MMP+Q+WKNLDP TGVHWEE EWW +IR +WPAY+K+ITP MG VAEMFRKWP Sbjct: 61 KGTIMMPTQSWKNLDPQTGVHWEEPKEWWQLIRDNWPAYNKDITPTNTMGQVAEMFRKWP 120 Query: 128 GAARSDHPARSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTS 187 GA RS HPARSVAA+G++++YLT+NHDLSNIFG SP+DKLY L+GYVLL GVG+DKNTS Sbjct: 121 GAKRSSHPARSVAALGRYSDYLTENHDLSNIFGKDSPIDKLYKLDGYVLLIGVGYDKNTS 180 Query: 188 LHLAEARADFPAKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQ 247 +HLA+A A++P K V +SSAVM+ G+R+W+TYET AV+ EDF +G +++K + + Sbjct: 181 IHLADAIAEYPGKHNVNESSAVMINGKREWITYETLAVDGEDFEDIGRDFEKAYQVNKIK 240 Query: 248 AGKAEIRFMEQRPLVDWATAWMEKNR 273 G EIRFM+QR +VD+A W+EK R Sbjct: 241 IGNGEIRFMKQRDIVDYAVKWIEKYR 266 >B1QUL3.1 B1QUL3_CLOBU Aminoglycoside N3'-acetyltransferase Length = 267 Score = 346 bits (888), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 158/266 (59%), Positives = 205/266 (77%) Query: 8 LTRSNETRMIFTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGE 67 + + + R I KEDLI+ F G+ +GQ I+VHTSL GF+ GGA+ I ALLE V + Sbjct: 1 MKNNEKERKIILKEDLIEGFKKLGVKEGQSIMVHTSLKSMGFVCGGAQIVIEALLESVKD 60 Query: 68 EGTLMMPSQTWKNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWP 127 +GT+MMP+Q+WKNLDP TGVHWEE EWW +IR +WPAY+K+ITP MG VAEMFRKWP Sbjct: 61 KGTIMMPTQSWKNLDPQTGVHWEEPKEWWQLIRDNWPAYNKDITPTNTMGQVAEMFRKWP 120 Query: 128 GAARSDHPARSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTS 187 GA RS HPARSVAA+G++++YLT+NHDLSNIFG SP+DKLY L+GYVLL GVG+DKNTS Sbjct: 121 GAKRSSHPARSVAALGRYSDYLTENHDLSNIFGKDSPIDKLYKLDGYVLLIGVGYDKNTS 180 Query: 188 LHLAEARADFPAKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQ 247 +HLA+A A++P K V +SSAVM+ G+R+W+TYET AV+ EDF +G +++K + + Sbjct: 181 IHLADAIAEYPGKHNVNESSAVMINGKREWITYETLAVDGEDFEDIGRDFEKAYQVNKIK 240 Query: 248 AGKAEIRFMEQRPLVDWATAWMEKNR 273 G EIRFM+QR +VD+A W+EK R Sbjct: 241 IGNGEIRFMKQRDIVDYAVKWIEKYR 266 >A6M2W6.1 A6M2W6_CLOB8 Aminoglycoside N(3')-acetyltransferase EC=2.3.1.81 Length = 263 Score = 321 bits (822), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 144/259 (55%), Positives = 193/259 (74%) Query: 15 RMIFTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGEEGTLMMP 74 R I K+D++ G+ GQ+I+VHTS+ FGF+ GG + I AL+E+VG++GT++MP Sbjct: 4 RKIVIKKDIVNALQQLGVKNGQNIIVHTSMKSFGFVCGGPQIIIEALIELVGKDGTIIMP 63 Query: 75 SQTWKNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSDH 134 +QTWKN+DP+ GVHWEE EWW +IR +WPAYDK+ITP MG VAEMFRKW G RSDH Sbjct: 64 TQTWKNMDPAYGVHWEEPIEWWDIIRDNWPAYDKDITPTNTMGAVAEMFRKWSGVLRSDH 123 Query: 135 PARSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTSLHLAEAR 194 P RS AA GK+A+YL NHDLS+IFG SP+ KLY L+GYVLL G G+DKNTS+HLA+ R Sbjct: 124 PVRSFAAWGKNAQYLINNHDLSDIFGESSPIGKLYDLDGYVLLLGTGYDKNTSIHLADER 183 Query: 195 ADFPAKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQAGKAEIR 254 AD+P+K +S A+M +G RKWVTY T V+ E+FI +G +++K+ + + G A++R Sbjct: 184 ADYPSKHMEKNSCAMMKDGERKWVTYSTLYVDGEEFIDIGIDFEKKYRVGNTKIGNADVR 243 Query: 255 FMEQRPLVDWATAWMEKNR 273 F+ QR +VD+A W+EKNR Sbjct: 244 FIRQREIVDFAVEWIEKNR 262 >C4GBE1.1 C4GBE1_9FIRM Putative uncharacterized protein Length = 261 Score = 291 bits (745), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 132/257 (51%), Positives = 178/257 (69%) Query: 17 IFTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGEEGTLMMPSQ 76 + K+D+++ GL KG ++VHTSL G++ GGA+ I AL+E+V EGT++MP+Q Sbjct: 4 VVCKKDIVQGLRGIGLKKGDIVMVHTSLKNIGYVCGGAQAVIEALMEVVTSEGTIVMPTQ 63 Query: 77 TWKNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSDHPA 136 +WKNLDP TGVHW+ W +IR++WPAYDKEITP MG VAEMFR+WPG+ R+DHPA Sbjct: 64 SWKNLDPETGVHWDADKSDWNIIRENWPAYDKEITPTNTMGAVAEMFRQWPGSIRTDHPA 123 Query: 137 RSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTSLHLAEARAD 196 RS+ A GKHA Y+T H LSNIFG GSP+ KLY L+ VLL GVG+DKNTS+HLA+ RA Sbjct: 124 RSLCAWGKHAAYITAEHSLSNIFGEGSPIAKLYELDAKVLLLGVGYDKNTSIHLADVRAT 183 Query: 197 FPAKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQAGKAEIRFM 256 +P K + SA++ +G R W Y T V+ DF +G +DK + G A+ RFM Sbjct: 184 YPGKHNCVEHSAILEKGVRVWKAYSTLFVDGADFEEIGSAFDKTGLASAGKVGNADSRFM 243 Query: 257 EQRPLVDWATAWMEKNR 273 QR +VD++ +W+E +R Sbjct: 244 RQRDIVDFSVSWIESHR 260 >A6CL21.1 A6CL21_9BACI Aminoglycoside N3'-acetyltransferase Length = 269 Score = 225 bits (573), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 115/255 (45%), Positives = 155/255 (60%), Gaps = 2/255 (0%) Query: 19 TKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGEEGTLMMPSQTW 78 T L+ F G+ KG ++VH+SLS G++ GGA + + AL+E VG+EGTL+MPSQ+ Sbjct: 15 TTSSLMDDFLRMGIEKGMTLIVHSSLSSLGWVCGGAVSVVTALMEAVGKEGTLIMPSQSA 74 Query: 79 KNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSDHPARS 138 N DP+ WWP IR PAYD + TPA GMGV+AE FR +PG RS HP S Sbjct: 75 DNSDPAEWQQPPVPEAWWPTIRDKMPAYDPDTTPARGMGVIAETFRSFPGVKRSLHPMYS 134 Query: 139 VAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTSLHLAEARADFP 198 A GKHAEY+ L FG SPL K+Y L+G VLL GV HD NTSLH AE P Sbjct: 135 FTAWGKHAEYILSEQPLEEGFGPRSPLAKIYELDGDVLLLGVTHDSNTSLHFAE--HSIP 192 Query: 199 AKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQAGKAEIRFMEQ 258 +++V +A+ G R W TYE + + F +LG+E++K+ +K G A+ R M+Q Sbjct: 193 HREKVQKGAAMKENGNRVWNTYEEILYDSDPFEKLGKEFEKQQPVKSAVIGNADCRLMKQ 252 Query: 259 RPLVDWATAWMEKNR 273 R VD+A W+++N+ Sbjct: 253 RAAVDFARNWLQQNQ 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,685,673,189 Number of extensions: 69475874 Number of successful extensions: 148561 Number of sequences better than 1.0e-05: 228 Number of HSP's gapped: 148296 Number of HSP's successfully gapped: 228 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)