BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0004.p (310 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1YLS8.1 B1YLS8_EXIS2 Diacylglycerol kinase catalytic region 251 5e-65 Q722E1.1 Q722E1_LISMF Putative uncharacterized protein 242 3e-62 C1L143.1 C1L143_LISMC Conserved hypothetical protein 242 3e-62 Q4ELW9.1 Q4ELW9_LISMO Putative uncharacterized protein 242 3e-62 Q92DP5.1 Q92DP5_LISIN Lin0768 protein 242 3e-62 >B1YLS8.1 B1YLS8_EXIS2 Diacylglycerol kinase catalytic region Length = 296 Score = 251 bits (642), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 127/295 (43%), Positives = 187/295 (63%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M++ +I+NPSSGKE + E L EV V+ T+KE DAT ++ A D Sbjct: 1 MSKVMLIINPSSGKELGKKHAELAEGTLAQRFTEVDVRFTEKELDATEFATQASLGHYDA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 +V++GGDGT++E I G++ QAH+P LGI+PLGTVND ARAL IP PEEA+ L + Sbjct: 61 VVAMGGDGTVNEVITGIAEQAHRPTLGIVPLGTVNDLARALHIPTDPEEAISILEDAPSR 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D G+ ++ F+NV+A G +AE++ VS + K+ LG++AYF EG+K ++ +P+ + Sbjct: 121 PLDIGKYDDHYFMNVIAVGLIAEAVEEVSVEQKTSLGSVAYFIEGLKAFKDHSPYPIRLT 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 + E++ GETPL + +LTNSVGGFE A +A VDDGL+H +I+K L FF+ L +L Sbjct: 181 AEEESFDGETPLLIISLTNSVGGFESFAKDARVDDGLLHVFIVKQLGFFDALQALPKLLT 240 Query: 255 GNLKNHKDVEYFTAGEVTISSSDTVRTNVDGEEGPALPIRLSVLPRHIRVVVPDS 309 G + + VEYFT EV + S + + N DG+ LP+ + VL +H+ V P S Sbjct: 241 GKISDADQVEYFTTKEVHVDSKERLPINADGDTAGHLPVTVQVLSQHLTVFAPVS 295 >Q722E1.1 Q722E1_LISMF Putative uncharacterized protein Length = 309 Score = 242 bits (618), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 124/294 (42%), Positives = 182/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E + Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N I+ Sbjct: 121 PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LTNSVGG E AP+A +DDG +H I+ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 >C1L143.1 C1L143_LISMC Conserved hypothetical protein Length = 309 Score = 242 bits (618), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 124/294 (42%), Positives = 182/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E + Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N I+ Sbjct: 121 PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LTNSVGG E AP+A +DDG +H I+ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 >Q4ELW9.1 Q4ELW9_LISMO Putative uncharacterized protein Length = 309 Score = 242 bits (618), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 124/294 (42%), Positives = 182/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E + Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N I+ Sbjct: 121 PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LTNSVGG E AP+A +DDG +H I+ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 >Q92DP5.1 Q92DP5_LISIN Lin0768 protein Length = 309 Score = 242 bits (618), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 122/294 (41%), Positives = 182/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E D Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWASEQGFDA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L + Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAKAI 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N ++ Sbjct: 121 PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKLE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LT SVGG E AP+A +DDG +H ++ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTKSVGGMESWAPDAKIDDGYLHVVVLTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,801,382,723 Number of extensions: 73059004 Number of successful extensions: 234477 Number of sequences better than 1.0e-05: 1435 Number of HSP's gapped: 232384 Number of HSP's successfully gapped: 1435 Length of query: 310 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 175 Effective length of database: 1,788,859,178 Effective search space: 313050356150 Effective search space used: 313050356150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)