BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0494.0007.p (239 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2XM53.1 C2XM53_BACCE CRISPR-associated protein Cas5 371 e-101 Q9KFY3.1 Q9KFY3_BACHD BH0337 protein 335 2e-90 C3BTZ8.1 C3BTZ8_9BACI CRISPR-associated protein Cas5 335 2e-90 C3FCD6.1 C3FCD6_BACTU CRISPR-associated protein Cas5 333 6e-90 Q5WBY1.1 Q5WBY1_BACSK Putative uncharacterized protein 332 1e-89 >C2XM53.1 C2XM53_BACCE CRISPR-associated protein Cas5 Length = 246 Score = 371 bits (952), Expect = e-101, Method: Compositional matrix adjust. Identities = 169/223 (75%), Positives = 205/223 (91%), Gaps = 1/223 (0%) Query: 1 MRNSIEFKVYGKYALFTDPLTKLGGEKLTYSVPTYQALKGIVESIYWKPTIHIIVEEVRI 60 +RNSIEF+V+G YALFTDPL K+GGEKLTY VPTYQA+KGIVESIYWKPT+ +IV+++RI Sbjct: 8 LRNSIEFEVFGDYALFTDPLMKMGGEKLTYQVPTYQAIKGIVESIYWKPTLLMIVDKIRI 67 Query: 61 MNAIRMESKGIRPMDYGGGNTLANYTYLKDVCYEVRAHFEFNMNRPDLEHDRNEHKHHNL 120 MNAI+MESKGIRP++YGGGNTLANYTYLK+V Y+V+AHF FN +RPDL DRNE+KHHN+ Sbjct: 68 MNAIKMESKGIRPIEYGGGNTLANYTYLKNVRYQVQAHFIFNPHRPDLAFDRNEYKHHNI 127 Query: 121 LKRSLQAGGRRDIFLGARECQAYVEPCVFGVERGFYNDY-GDIHLGSMVHGINYPDETGR 179 LKRSL+ GGRRDIFLG RECQ YVEPCVFG GFY++Y GDIHLG+MVHG+NYPDETGR Sbjct: 128 LKRSLKVGGRRDIFLGTRECQGYVEPCVFGEGEGFYDNYGGDIHLGTMVHGLNYPDETGR 187 Query: 180 DEMEVRLWSPVMRDGIIRFIRPEECTQVRRIAEMKPKSFNETN 222 +E+EVRLW+PVMRDGII+FIRPEECT++R+I++M+PK F+ +N Sbjct: 188 NELEVRLWNPVMRDGIIQFIRPEECTKIRKISKMEPKIFDSSN 230 >Q9KFY3.1 Q9KFY3_BACHD BH0337 protein Length = 236 Score = 335 bits (860), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 151/222 (68%), Positives = 188/222 (84%) Query: 1 MRNSIEFKVYGKYALFTDPLTKLGGEKLTYSVPTYQALKGIVESIYWKPTIHIIVEEVRI 60 MRN ++F+++G YALFTDPLTK+GGEKL+YSVPTYQALKGI ESIYWKPTI +++E+R+ Sbjct: 1 MRNEVQFELFGDYALFTDPLTKIGGEKLSYSVPTYQALKGIAESIYWKPTIVFVIDELRV 60 Query: 61 MNAIRMESKGIRPMDYGGGNTLANYTYLKDVCYEVRAHFEFNMNRPDLEHDRNEHKHHNL 120 M I+MESKG+RP++YGGGNTLA+YTYLKDV Y+V+AHFEFN++RPDL DRNE KH+++ Sbjct: 61 MKPIQMESKGVRPIEYGGGNTLAHYTYLKDVHYQVKAHFEFNLHRPDLAFDRNEGKHYSI 120 Query: 121 LKRSLQAGGRRDIFLGARECQAYVEPCVFGVERGFYNDYGDIHLGSMVHGINYPDETGRD 180 L+RSL+AGGRRDIFLGARECQ YV PC FG GFY+ G HLG+MVHG NYPDETG+ Sbjct: 121 LQRSLKAGGRRDIFLGARECQGYVAPCEFGSGDGFYDGQGKYHLGTMVHGFNYPDETGQH 180 Query: 181 EMEVRLWSPVMRDGIIRFIRPEECTQVRRIAEMKPKSFNETN 222 +++VRLWS VM +G I+F RPE+C VR + EM+PK FN N Sbjct: 181 QLDVRLWSAVMENGYIQFPRPEDCPIVRPVKEMEPKIFNPDN 222 >C3BTZ8.1 C3BTZ8_9BACI CRISPR-associated protein Cas5 Length = 219 Score = 335 bits (859), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 152/202 (75%), Positives = 185/202 (91%), Gaps = 1/202 (0%) Query: 22 KLGGEKLTYSVPTYQALKGIVESIYWKPTIHIIVEEVRIMNAIRMESKGIRPMDYGGGNT 81 K+GGEK +Y VPTYQA+KGIVESIYWKPT+ ++V+++RIMNAI+MESKGIRP++YGGGNT Sbjct: 2 KMGGEKFSYQVPTYQAIKGIVESIYWKPTLLMVVDKIRIMNAIKMESKGIRPIEYGGGNT 61 Query: 82 LANYTYLKDVCYEVRAHFEFNMNRPDLEHDRNEHKHHNLLKRSLQAGGRRDIFLGARECQ 141 LANYTYLKDV Y+++AHF FN +RPDL D NE KHHN+LKRSL+AGGRRDIFLG RECQ Sbjct: 62 LANYTYLKDVRYQIKAHFIFNPHRPDLAFDHNEFKHHNILKRSLKAGGRRDIFLGTRECQ 121 Query: 142 AYVEPCVFGVERGFYNDY-GDIHLGSMVHGINYPDETGRDEMEVRLWSPVMRDGIIRFIR 200 YVEPCVFG +GFY++Y GDIHLG+MVHG+NYPDETGR+E+EVRLW+PVMRDGII+FIR Sbjct: 122 GYVEPCVFGEGKGFYDNYGGDIHLGTMVHGLNYPDETGRNELEVRLWNPVMRDGIIQFIR 181 Query: 201 PEECTQVRRIAEMKPKSFNETN 222 PEECT+VR+IA+M+PK F+ +N Sbjct: 182 PEECTKVRKIAKMEPKVFDRSN 203 >C3FCD6.1 C3FCD6_BACTU CRISPR-associated protein Cas5 Length = 220 Score = 333 bits (855), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 149/202 (73%), Positives = 187/202 (92%), Gaps = 1/202 (0%) Query: 22 KLGGEKLTYSVPTYQALKGIVESIYWKPTIHIIVEEVRIMNAIRMESKGIRPMDYGGGNT 81 K+GGEKL+Y VPTYQA+KGIVESIYWKPT+ ++V+++RIMNAI+MESKGIRP++YGGGNT Sbjct: 2 KMGGEKLSYQVPTYQAIKGIVESIYWKPTLLMVVDKIRIMNAIKMESKGIRPIEYGGGNT 61 Query: 82 LANYTYLKDVCYEVRAHFEFNMNRPDLEHDRNEHKHHNLLKRSLQAGGRRDIFLGARECQ 141 LANYTYLKDV Y+V+A+F FN +RPDL DRNE KHHN+LKR+L+AGGRRDIFLG RECQ Sbjct: 62 LANYTYLKDVRYQVKAYFIFNPHRPDLAFDRNEFKHHNILKRALKAGGRRDIFLGTRECQ 121 Query: 142 AYVEPCVFGVERGFYNDY-GDIHLGSMVHGINYPDETGRDEMEVRLWSPVMRDGIIRFIR 200 YVEPCVFG ++GFY+DY GDIHLG+MVHG+NYPDETGR+++EVRLW+PV+RDGII+FI Sbjct: 122 GYVEPCVFGEDKGFYDDYGGDIHLGTMVHGLNYPDETGRNQLEVRLWNPVIRDGIIQFIL 181 Query: 201 PEECTQVRRIAEMKPKSFNETN 222 PEEC+Q+R+I++M+PK F+ +N Sbjct: 182 PEECSQIRKISKMEPKVFDRSN 203 >Q5WBY1.1 Q5WBY1_BACSK Putative uncharacterized protein Length = 236 Score = 332 bits (852), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 148/218 (67%), Positives = 185/218 (84%) Query: 1 MRNSIEFKVYGKYALFTDPLTKLGGEKLTYSVPTYQALKGIVESIYWKPTIHIIVEEVRI 60 M+N I+F+V+G YALFTDPLTK+GGEKL+YSVPTYQALKGIVESIYWKPTI ++++R+ Sbjct: 1 MKNEIQFEVFGDYALFTDPLTKIGGEKLSYSVPTYQALKGIVESIYWKPTIIFTIDKLRV 60 Query: 61 MNAIRMESKGIRPMDYGGGNTLANYTYLKDVCYEVRAHFEFNMNRPDLEHDRNEHKHHNL 120 M I+MESKG+RP+DYGGGNTLA+YTYLKDV Y+V+AHF+FN NRPDL DRNE KH+++ Sbjct: 61 MKPIQMESKGVRPIDYGGGNTLAHYTYLKDVHYQVKAHFDFNFNRPDLAFDRNEGKHYSI 120 Query: 121 LKRSLQAGGRRDIFLGARECQAYVEPCVFGVERGFYNDYGDIHLGSMVHGINYPDETGRD 180 L+RSL+AGGRRDIFLG RECQ YV PC FG G+Y+ G+ LG+MVHG NYPDETG++ Sbjct: 121 LQRSLKAGGRRDIFLGTRECQGYVAPCEFGSGSGYYDGQGEYRLGTMVHGFNYPDETGQN 180 Query: 181 EMEVRLWSPVMRDGIIRFIRPEECTQVRRIAEMKPKSF 218 +++VRLW VM DG I+F +PE+CT +R I EM+PK F Sbjct: 181 QLDVRLWPAVMNDGYIKFPKPEDCTMIRPIKEMEPKEF 218 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,418,768,530 Number of extensions: 60276385 Number of successful extensions: 100992 Number of sequences better than 1.0e-05: 93 Number of HSP's gapped: 100932 Number of HSP's successfully gapped: 93 Length of query: 239 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 107 Effective length of database: 1,817,122,223 Effective search space: 194432077861 Effective search space used: 194432077861 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)