BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0494.0005.p (286 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2XM51.1 C2XM51_BACCE CRISPR-associated protein, Csd2 487 e-136 C3FCD3.1 C3FCD3_BACTU CRISPR-associated protein, Csd2 477 e-133 Q9KFY1.1 Q9KFY1_BACHD BH0339 protein 466 e-130 C3BU00.1 C3BU00_9BACI CRISPR-associated protein, Csd2 466 e-129 Q5WBY3.1 Q5WBY3_BACSK Putative uncharacterized protein 464 e-129 >C2XM51.1 C2XM51_BACCE CRISPR-associated protein, Csd2 Length = 286 Score = 487 bits (1253), Expect = e-136, Method: Compositional matrix adjust. Identities = 231/286 (80%), Positives = 258/286 (90%) Query: 1 MTILDHKIDFAVIVSVTKANPNGDPLNGNRPRQNYDGYGEISDVALKRKIRNRLQDMGES 60 M LDHKIDFAV++SVTKANPNGDPLNGNRPRQNYDG+GEISDVA+KRKIRNRLQDM ES Sbjct: 1 MKTLDHKIDFAVVLSVTKANPNGDPLNGNRPRQNYDGHGEISDVAIKRKIRNRLQDMEES 60 Query: 61 IFVQSNDRKSDSYNSLRERADANAELDKILKAKGSSNEEFARIACQEWLDVRSFGQVFAF 120 IFVQSNDRK D +NSLRERA+ NA+L K LK+K + +E+FA IAC+EW+DVRSFGQVFAF Sbjct: 61 IFVQSNDRKVDQFNSLRERAETNADLVKALKSKDNPHEQFAAIACKEWIDVRSFGQVFAF 120 Query: 121 KADKGAGVSVGVRGPVSIHTATSVSPIDITSMQITKSVNSEPGKDRGSDTMGMKHRVDFG 180 K G GVSVGVRGPVSIHTATSV PIDITSMQITKSVNSEPGKD+GSDTMGMKHRVDFG Sbjct: 121 KGGAGKGVSVGVRGPVSIHTATSVDPIDITSMQITKSVNSEPGKDKGSDTMGMKHRVDFG 180 Query: 181 VYVFYGSINTQLAEKTGFSNEDAEKIKQVLVTLFENDASAARPDGSMEVHKVYWWEHNSK 240 VYVFYGSINTQLAEKTGF+NEDAEKIK LVTLFENDASAARP+GS+EVHKV WW+HNSK Sbjct: 181 VYVFYGSINTQLAEKTGFTNEDAEKIKNALVTLFENDASAARPEGSIEVHKVCWWDHNSK 240 Query: 241 LGQYSSAKVHRSLVVRPKVDDPKTIADYEIVVSEMEGLKAEVIDGI 286 LGQYSSAKVHR L V+ +D+PKTI DY ++V E++ LK EVI+G+ Sbjct: 241 LGQYSSAKVHRLLEVKRNIDEPKTIDDYTVLVHELDDLKVEVINGM 286 >C3FCD3.1 C3FCD3_BACTU CRISPR-associated protein, Csd2 Length = 286 Score = 477 bits (1228), Expect = e-133, Method: Compositional matrix adjust. Identities = 224/286 (78%), Positives = 256/286 (89%) Query: 1 MTILDHKIDFAVIVSVTKANPNGDPLNGNRPRQNYDGYGEISDVALKRKIRNRLQDMGES 60 MTIL+HKIDFAV++SVTKANPNGDPLNGNRPRQNYDGYGEISDVA+KRKIRNRLQDM ES Sbjct: 1 MTILNHKIDFAVVLSVTKANPNGDPLNGNRPRQNYDGYGEISDVAIKRKIRNRLQDMEES 60 Query: 61 IFVQSNDRKSDSYNSLRERADANAELDKILKAKGSSNEEFARIACQEWLDVRSFGQVFAF 120 IFVQSNDR+ D +NSL+ERA+ N +L K+LK++ + E+FA IAC+EW+DVRSFGQVFAF Sbjct: 61 IFVQSNDRRVDQFNSLKERAENNVDLIKVLKSEENPYEQFAAIACKEWIDVRSFGQVFAF 120 Query: 121 KADKGAGVSVGVRGPVSIHTATSVSPIDITSMQITKSVNSEPGKDRGSDTMGMKHRVDFG 180 K G GVSVGVRGPVSIHTATS+ IDITSMQITKSVNSEPGKD+ SDTMGMKHRVDFG Sbjct: 121 KGGAGKGVSVGVRGPVSIHTATSIDVIDITSMQITKSVNSEPGKDKSSDTMGMKHRVDFG 180 Query: 181 VYVFYGSINTQLAEKTGFSNEDAEKIKQVLVTLFENDASAARPDGSMEVHKVYWWEHNSK 240 VYVFYGSINTQLAEKTGF+NEDAEKIK LVTLFENDASAARP+GS+E+ +V WWEHNSK Sbjct: 181 VYVFYGSINTQLAEKTGFTNEDAEKIKSALVTLFENDASAARPEGSIEIQRVCWWEHNSK 240 Query: 241 LGQYSSAKVHRSLVVRPKVDDPKTIADYEIVVSEMEGLKAEVIDGI 286 LGQYSSAKVHR L + +D+PKTI DY I+V E++GLK ++IDG+ Sbjct: 241 LGQYSSAKVHRLLDINRNIDEPKTIDDYTILVHELDGLKVDIIDGL 286 >Q9KFY1.1 Q9KFY1_BACHD BH0339 protein Length = 283 Score = 466 bits (1200), Expect = e-130, Method: Compositional matrix adjust. Identities = 223/285 (78%), Positives = 251/285 (88%), Gaps = 3/285 (1%) Query: 1 MTILDHKIDFAVIVSVTKANPNGDPLNGNRPRQNYDGYGEISDVALKRKIRNRLQDMGES 60 MTILDHKIDFAVI+SVTKANPNGDPLNGNRPRQNYDG+GEISDVA+KRKIRNRL DM E Sbjct: 1 MTILDHKIDFAVILSVTKANPNGDPLNGNRPRQNYDGHGEISDVAIKRKIRNRLLDMEEP 60 Query: 61 IFVQSNDRKSDSYNSLRERADANAELDKILKAKGSSNEEFARIACQEWLDVRSFGQVFAF 120 IFVQS+DRK+DS+ SLR+RAD+N EL K+LKAK +S +EFA+IACQEW+DVRSFGQVFAF Sbjct: 61 IFVQSDDRKADSFKSLRDRADSNPELAKMLKAKNASVDEFAKIACQEWMDVRSFGQVFAF 120 Query: 121 KADKGAGVSVGVRGPVSIHTATSVSPIDITSMQITKSVNSEPGKDRGSDTMGMKHRVDFG 180 K G+ +SVGVRGPVSIHTATS+ PIDI S QITKSVNS G R SDTMGMKHRVDFG Sbjct: 121 K---GSNLSVGVRGPVSIHTATSIDPIDIVSTQITKSVNSVTGDKRSSDTMGMKHRVDFG 177 Query: 181 VYVFYGSINTQLAEKTGFSNEDAEKIKQVLVTLFENDASAARPDGSMEVHKVYWWEHNSK 240 VYVF GSINTQLAEKTGF+NEDAEKIK+ L+TLFEND+S+ARPDGSMEVHKVYWWEH+SK Sbjct: 178 VYVFKGSINTQLAEKTGFTNEDAEKIKRALITLFENDSSSARPDGSMEVHKVYWWEHSSK 237 Query: 241 LGQYSSAKVHRSLVVRPKVDDPKTIADYEIVVSEMEGLKAEVIDG 285 LGQYSSAKVHRSL + K D PK+ DY + + E++GL EVIDG Sbjct: 238 LGQYSSAKVHRSLKIESKTDTPKSFDDYAVELYELDGLGVEVIDG 282 >C3BU00.1 C3BU00_9BACI CRISPR-associated protein, Csd2 Length = 286 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 228/286 (79%), Positives = 256/286 (89%) Query: 1 MTILDHKIDFAVIVSVTKANPNGDPLNGNRPRQNYDGYGEISDVALKRKIRNRLQDMGES 60 MT L+HKIDFAV++SVTKANPNGDPLNGNRPRQNYDG+GEISDVA+KRKIRNRLQDM ES Sbjct: 1 MTTLNHKIDFAVVLSVTKANPNGDPLNGNRPRQNYDGHGEISDVAIKRKIRNRLQDMEES 60 Query: 61 IFVQSNDRKSDSYNSLRERADANAELDKILKAKGSSNEEFARIACQEWLDVRSFGQVFAF 120 IFVQSNDRK D +NSLRERA+ N +L K LK+K + +E+FA +AC+EW+DVRSFGQVFAF Sbjct: 61 IFVQSNDRKVDQFNSLRERAETNVDLVKALKSKENPHEQFASVACKEWIDVRSFGQVFAF 120 Query: 121 KADKGAGVSVGVRGPVSIHTATSVSPIDITSMQITKSVNSEPGKDRGSDTMGMKHRVDFG 180 K G GVSVGVRGPVSIHTATSV PIDITSMQITKSVNSEPG D+GSDTMGMKHRVDFG Sbjct: 121 KGGSGKGVSVGVRGPVSIHTATSVDPIDITSMQITKSVNSEPGNDKGSDTMGMKHRVDFG 180 Query: 181 VYVFYGSINTQLAEKTGFSNEDAEKIKQVLVTLFENDASAARPDGSMEVHKVYWWEHNSK 240 VYVFYGSINTQLAEKTGF+NEDA KIK LVTLFENDASAARP+GS+EVHKV WWEHNSK Sbjct: 181 VYVFYGSINTQLAEKTGFTNEDALKIKNALVTLFENDASAARPEGSIEVHKVCWWEHNSK 240 Query: 241 LGQYSSAKVHRSLVVRPKVDDPKTIADYEIVVSEMEGLKAEVIDGI 286 LGQYSSAKVHR L ++ +D+PKTI DY I+V E++GLK +IDG+ Sbjct: 241 LGQYSSAKVHRLLDIKRNIDEPKTIDDYTILVHELDGLKVAIIDGL 286 >Q5WBY3.1 Q5WBY3_BACSK Putative uncharacterized protein Length = 285 Score = 464 bits (1193), Expect = e-129, Method: Compositional matrix adjust. Identities = 222/287 (77%), Positives = 252/287 (87%), Gaps = 5/287 (1%) Query: 1 MTILDHKIDFAVIVSVTKANPNGDPLNGNRPRQNYDGYGEISDVALKRKIRNRLQDMGES 60 M+ LDHKIDFAVI+SV+KANPNGDPLNGNRPRQNYDG+GEISDVA+KRKIRNRLQDMGE Sbjct: 1 MSTLDHKIDFAVILSVSKANPNGDPLNGNRPRQNYDGHGEISDVAIKRKIRNRLQDMGEP 60 Query: 61 IFVQSNDRKSDSYNSLRERADANAELDKILKAKGSSNEEFARIACQEWLDVRSFGQVFAF 120 +FVQS+DRK DS+ SLRERAD+N EL K+LKAK +S+++FA+IAC+EW+DVRSFGQVFAF Sbjct: 61 VFVQSDDRKVDSHKSLRERADSNPELAKMLKAKNASSDDFAQIACEEWIDVRSFGQVFAF 120 Query: 121 KADKGAGVSVGVRGPVSIHTATSVSPIDITSMQITKSVNSEPGKDRGSDTMGMKHRVDFG 180 K G+ +SVGVRGPVSIHTATS+ PIDI S QITKSVNS G R SDTMGMKHRVDFG Sbjct: 121 K---GSNLSVGVRGPVSIHTATSIDPIDIVSTQITKSVNSVTGDKRSSDTMGMKHRVDFG 177 Query: 181 VYVFYGSINTQLAEKTGFSNEDAEKIKQVLVTLFENDASAARPDGSMEVHKVYWWEHNSK 240 VYVF GSINTQLAEKTGF+NEDAEKIKQ LVTLFEND+S+ARPDGSMEVHKVYWWEH+SK Sbjct: 178 VYVFKGSINTQLAEKTGFTNEDAEKIKQALVTLFENDSSSARPDGSMEVHKVYWWEHSSK 237 Query: 241 LGQYSSAKVHRSLVVRPKVDDPKTI--ADYEIVVSEMEGLKAEVIDG 285 LGQYSSAKVHRSL V K D PK +Y + +S++EGL EV+DG Sbjct: 238 LGQYSSAKVHRSLKVEAKTDSPKAFDEENYSVELSDLEGLSVEVLDG 284 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,636,974,198 Number of extensions: 65284016 Number of successful extensions: 125781 Number of sequences better than 1.0e-05: 185 Number of HSP's gapped: 125294 Number of HSP's successfully gapped: 186 Length of query: 286 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 152 Effective length of database: 1,798,280,193 Effective search space: 273338589336 Effective search space used: 273338589336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)