BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0494.0004.p (218 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C3BU01.1 C3BU01_9BACI CRISPR-associated protein Cas4 349 1e-94 Q5WBY4.1 Q5WBY4_BACSK RecB family exonuclease 329 1e-88 B1YHA6.1 B1YHA6_EXIS2 CRISPR-associated protein Cas4 281 2e-74 C1TE34.1 C1TE34_9FIRM CRISPR-associated exonuclease, Cas4 family 257 4e-67 A6TNS5.1 A6TNS5_ALKMQ CRISPR-associated protein Cas4 253 7e-66 >C3BU01.1 C3BU01_9BACI CRISPR-associated protein Cas4 Length = 209 Score = 349 bits (895), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 159/209 (76%), Positives = 188/209 (89%) Query: 10 MLSGIQHFHFCKRQWALIHIEQQWEENVRTIEGQHLHRNADQPLIREKRVDKIIVRAMPV 69 MLSGIQHF FCKRQWALIHIEQQWEENVRTIEG++LH+ ADQP I+EKR +K+ VRA+P+ Sbjct: 1 MLSGIQHFQFCKRQWALIHIEQQWEENVRTIEGKYLHQKADQPFIKEKRGNKLTVRALPI 60 Query: 70 KSRELNISGICDVVEFIRDDSGVEINGADGKYAAYPVEYKRGKPKREDEDILQLAAQAMC 129 KS EL I+GICDVVEFI+D+SG+EINGA+GKY A+P+EYKRGKPK D D+LQL AQA+C Sbjct: 61 KSNELKITGICDVVEFIKDNSGIEINGAEGKYIAHPIEYKRGKPKMNDSDVLQLTAQAIC 120 Query: 130 LEEMLLCEVSTGYIFYNEIKHRIEVPLTAELKANVKAIVEVMQSYYHRRHTPKVKTGPFC 189 LEEMLLCE++TGYIFYNEIKHRIEVPLTA+ K V++IV+ M YY R+HTPKVKTG FC Sbjct: 121 LEEMLLCEINTGYIFYNEIKHRIEVPLTADDKNKVRSIVKEMYDYYKRKHTPKVKTGLFC 180 Query: 190 KSCSLQHICLPKLMNKQSVKSYVEGKIRE 218 K+CSLQ++CLPKLMNK+SVKSY+EGKI E Sbjct: 181 KNCSLQNVCLPKLMNKRSVKSYIEGKIHE 209 >Q5WBY4.1 Q5WBY4_BACSK RecB family exonuclease Length = 210 Score = 329 bits (843), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 147/210 (70%), Positives = 177/210 (84%) Query: 9 LMLSGIQHFHFCKRQWALIHIEQQWEENVRTIEGQHLHRNADQPLIREKRVDKIIVRAMP 68 +MLSG+QHF FCKRQWALIHIEQQWEENVRTIEGQHLH+ ADQP I+EKR K+ VRAMP Sbjct: 1 MMLSGLQHFQFCKRQWALIHIEQQWEENVRTIEGQHLHKKADQPFIKEKRGSKLTVRAMP 60 Query: 69 VKSRELNISGICDVVEFIRDDSGVEINGADGKYAAYPVEYKRGKPKREDEDILQLAAQAM 128 ++S++L ISGICDVVEF+ D GVE++G G Y A+PVEYKRGKPK+ DEDI+QL AQA+ Sbjct: 61 IQSKQLQISGICDVVEFVEDPDGVELSGVSGTYQAFPVEYKRGKPKKGDEDIVQLVAQAI 120 Query: 129 CLEEMLLCEVSTGYIFYNEIKHRIEVPLTAELKANVKAIVEVMQSYYHRRHTPKVKTGPF 188 CLEEML+C + GY+FYNEIKHR+EV +T L+ V + + M YY R+TPKVKTGPF Sbjct: 121 CLEEMLVCRIDKGYLFYNEIKHRVEVAITDSLREKVVEMAKEMHHYYDNRYTPKVKTGPF 180 Query: 189 CKSCSLQHICLPKLMNKQSVKSYVEGKIRE 218 CK+CSLQHICLPKLMNK+SVKSY+EG++ E Sbjct: 181 CKNCSLQHICLPKLMNKRSVKSYIEGRLSE 210 >B1YHA6.1 B1YHA6_EXIS2 CRISPR-associated protein Cas4 Length = 220 Score = 281 bits (720), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 135/214 (63%), Positives = 157/214 (73%) Query: 5 DEDELMLSGIQHFHFCKRQWALIHIEQQWEENVRTIEGQHLHRNADQPLIREKRVDKIIV 64 D+ L+LSGIQHF FC RQW LIHIEQQW ENVRTIEGQ LH+ AD P REKR +K+IV Sbjct: 7 DDTYLLLSGIQHFQFCPRQWTLIHIEQQWVENVRTIEGQALHQKADDPFQREKRKEKLIV 66 Query: 65 RAMPVKSRELNISGICDVVEFIRDDSGVEINGADGKYAAYPVEYKRGKPKREDEDILQLA 124 R M +KS +LNISG CDVVEFI D SGV I+G G + PVEYKRGKPK D LQLA Sbjct: 67 RGMAIKSEQLNISGQCDVVEFIEDASGVPIHGTPGTFIPVPVEYKRGKPKVNPADELQLA 126 Query: 125 AQAMCLEEMLLCEVSTGYIFYNEIKHRIEVPLTAELKANVKAIVEVMQSYYHRRHTPKVK 184 AQAMCLEEMLLC V GY++Y+E KHR +P+T EL+ V A V M YY RRHTP+ K Sbjct: 127 AQAMCLEEMLLCAVPIGYLYYHETKHRHPIPITKELRDLVLATVTKMNDYYSRRHTPRAK 186 Query: 185 TGPFCKSCSLQHICLPKLMNKQSVKSYVEGKIRE 218 GP CKSCSLQ ICLP+L ++SV Y+ ++ E Sbjct: 187 IGPHCKSCSLQEICLPRLTERESVADYMNRRLWE 220 >C1TE34.1 C1TE34_9FIRM CRISPR-associated exonuclease, Cas4 family Length = 225 Score = 257 bits (657), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 124/218 (56%), Positives = 155/218 (71%) Query: 1 MEYDDEDELMLSGIQHFHFCKRQWALIHIEQQWEENVRTIEGQHLHRNADQPLIREKRVD 60 MEY++ED L+LSGIQHF FCKRQWALIHIEQQW+EN+RT+EG LH I+EKR D Sbjct: 3 MEYNEEDFLLLSGIQHFSFCKRQWALIHIEQQWQENLRTVEGGILHEKTHDNTIKEKRGD 62 Query: 61 KIIVRAMPVKSRELNISGICDVVEFIRDDSGVEINGADGKYAAYPVEYKRGKPKREDEDI 120 II R+M + SR+L I+G CDVVEF + GV + G DG Y PVEYKRGKPK+++ D+ Sbjct: 63 LIISRSMAIFSRKLGITGACDVVEFHKSRDGVTLYGRDGTYKPVPVEYKRGKPKQDNMDV 122 Query: 121 LQLAAQAMCLEEMLLCEVSTGYIFYNEIKHRIEVPLTAELKANVKAIVEVMQSYYHRRHT 180 LQL AQAMCLEEMLLC + G++FY E K R++V L E++ VKAI + M Y RR+T Sbjct: 123 LQLCAQAMCLEEMLLCTIPEGFLFYGEPKRRLKVLLDEEIREQVKAICQEMHELYDRRYT 182 Query: 181 PKVKTGPFCKSCSLQHICLPKLMNKQSVKSYVEGKIRE 218 PKVK CK+CSL +CLPKL S +Y++ I E Sbjct: 183 PKVKPTKGCKACSLSELCLPKLCKNPSALAYMKKTISE 220 >A6TNS5.1 A6TNS5_ALKMQ CRISPR-associated protein Cas4 Length = 222 Score = 253 bits (646), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 120/218 (55%), Positives = 151/218 (69%) Query: 1 MEYDDEDELMLSGIQHFHFCKRQWALIHIEQQWEENVRTIEGQHLHRNADQPLIREKRVD 60 MEY +ED ++LSGIQHF FC+RQWALIHIEQQW+EN+RTIEGQ LH +REKR D Sbjct: 2 MEYKEEDFILLSGIQHFSFCRRQWALIHIEQQWQENLRTIEGQILHERTHDNTVREKRGD 61 Query: 61 KIIVRAMPVKSRELNISGICDVVEFIRDDSGVEINGADGKYAAYPVEYKRGKPKREDEDI 120 I+ R M + SR L I+G CDVVE + GV I G DG Y P+EYKRGKPK ++ DI Sbjct: 62 LIVSRGMAIFSRSLGITGACDVVELHKASDGVSIFGRDGNYKPVPIEYKRGKPKEDESDI 121 Query: 121 LQLAAQAMCLEEMLLCEVSTGYIFYNEIKHRIEVPLTAELKANVKAIVEVMQSYYHRRHT 180 LQL AQAMCLEEMLLCE+ ++FY E K R++V L EL+ V+ IV+ + Y +R+T Sbjct: 122 LQLCAQAMCLEEMLLCEIPEAFLFYGETKRRLKVTLEDELRQRVQTIVKEIHELYDKRYT 181 Query: 181 PKVKTGPFCKSCSLQHICLPKLMNKQSVKSYVEGKIRE 218 PKVK CK+CSL IC+PKL S +Y++ + E Sbjct: 182 PKVKPSKRCKACSLAEICMPKLCKNPSAVNYIKKNLLE 219 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,288,106,061 Number of extensions: 49682325 Number of successful extensions: 105753 Number of sequences better than 1.0e-05: 161 Number of HSP's gapped: 105559 Number of HSP's successfully gapped: 161 Length of query: 218 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 88 Effective length of database: 1,835,964,253 Effective search space: 161564854264 Effective search space used: 161564854264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)