BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0494.0003.p (343 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KFX9.1 Q9KFX9_BACHD BH0341 protein 529 e-148 Q5WBY5.1 Q5WBY5_BACSK Putative uncharacterized protein 521 e-146 B1YHA7.1 B1YHA7_EXIS2 CRISPR-associated protein Cas1 493 e-137 C3BU02.1 C3BU02_9BACI CRISPR-associated protein, Cas1 471 e-131 C0BZ41.1 C0BZ41_9CLOT Putative uncharacterized protein 466 e-129 >Q9KFX9.1 Q9KFX9_BACHD BH0341 protein Length = 343 Score = 529 bits (1362), Expect = e-148, Method: Compositional matrix adjust. Identities = 249/343 (72%), Positives = 285/343 (83%) Query: 1 MKKLLNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYC 60 MKKLLNTLY+TQP YLSLDGDNVVLLKE+EKLGR PLHNLE+IV FGYTGASPALMGYC Sbjct: 1 MKKLLNTLYVTQPDTYLSLDGDNVVLLKEQEKLGRLPLHNLEAIVGFGYTGASPALMGYC 60 Query: 61 ADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHK 120 A+ NIS+ FLT NGR+LARV G+SRGNVVLRK QY +SE++ S KIARNFI GK+YN K Sbjct: 61 AERNISITFLTKNGRFLARVVGESRGNVVLRKTQYRISENDQESTKIARNFITGKVYNSK 120 Query: 121 WMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQM 180 WMLERMTR++PLRVNVE FK TSQ LS ++ E+R C+ L+ LRG EGQAA Y F+QM Sbjct: 121 WMLERMTREHPLRVNVEQFKATSQLLSVMMQEIRNCDSLESLRGWEGQAAINYNKVFDQM 180 Query: 181 ILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGR 240 ILQQKE+F FH R+RRPP DNVNAMLS AYTLL +D A+ALE VGLDAYVGF+H+DRPGR Sbjct: 181 ILQQKEEFAFHGRSRRPPKDNVNAMLSFAYTLLANDVAAALETVGLDAYVGFMHQDRPGR 240 Query: 241 VSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKK 300 SLALD+MEELRG++AD+FVL+LIN+K ++ F KKENGAV+MTDEARK FL AWQ+KK Sbjct: 241 ASLALDLMEELRGLYADRFVLSLINRKEMTADGFYKKENGAVLMTDEARKTFLKAWQTKK 300 Query: 301 QETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 QE ITHPYLGEK+SWGLVP DLDEYPPFLWK Sbjct: 301 QEKITHPYLGEKMSWGLVPYVQALLLARFLRGDLDEYPPFLWK 343 >Q5WBY5.1 Q5WBY5_BACSK Putative uncharacterized protein Length = 343 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 251/343 (73%), Positives = 287/343 (83%) Query: 1 MKKLLNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYC 60 MKKLLNTLY+TQP YLSLDGDNVVLLKE+EKLGR PLHNLESIV+FGYTGASPALMGYC Sbjct: 1 MKKLLNTLYVTQPDTYLSLDGDNVVLLKEQEKLGRLPLHNLESIVSFGYTGASPALMGYC 60 Query: 61 ADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHK 120 AD NIS+ FLT NGR+LARV G+SRGNVVLRK QY +SE E S IARNFIIGKIYN+K Sbjct: 61 ADRNISITFLTKNGRFLARVVGESRGNVVLRKTQYRISESEKESTNIARNFIIGKIYNNK 120 Query: 121 WMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQM 180 WMLERMTR++PLRV+VE FK SQ LS+++ EVR+C+ L+ LRG EGQAA Y FNQM Sbjct: 121 WMLERMTREHPLRVDVEQFKSISQLLSTILQEVRSCDNLESLRGWEGQAAINYNKVFNQM 180 Query: 181 ILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGR 240 ILQQK+ F FH R+RRPP DNVNAMLS AYTLL +D A+ALE VGLDAYVGF+H+DRPGR Sbjct: 181 ILQQKDSFTFHGRSRRPPKDNVNAMLSFAYTLLANDAAAALETVGLDAYVGFMHQDRPGR 240 Query: 241 VSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKK 300 SLALD+MEELRG++AD+FVL+LIN+K ++ DF KKENGAV+M+D+ARK FL AWQ+KK Sbjct: 241 ASLALDLMEELRGIYADRFVLSLINRKEVTADDFYKKENGAVLMSDDARKSFLKAWQTKK 300 Query: 301 QETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 QE I+HPYLGEKISWGLVP DLDEYPPFLWK Sbjct: 301 QEKISHPYLGEKISWGLVPYAQALLLARFLRGDLDEYPPFLWK 343 >B1YHA7.1 B1YHA7_EXIS2 CRISPR-associated protein Cas1 Length = 343 Score = 493 bits (1268), Expect = e-137, Method: Compositional matrix adjust. Identities = 231/343 (67%), Positives = 275/343 (80%) Query: 1 MKKLLNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYC 60 MKKLLNTL+I Q YL+LDG NV++ + + LGR PLHNLE IV FGYTGASPALMG+C Sbjct: 1 MKKLLNTLFINQSDSYLALDGGNVLVKADGQVLGRVPLHNLEGIVAFGYTGASPALMGHC 60 Query: 61 ADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHK 120 A++ I+L F+T NGR+LARV G+SRGNVVLRK QY S+DEV SA++A+NFI+GK+YN K Sbjct: 61 AENGIALTFMTKNGRFLARVIGESRGNVVLRKTQYRRSDDEVASARLAKNFILGKLYNQK 120 Query: 121 WMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQM 180 WMLERMTRDYPLR++VE FK TSQ ++ + E++ CE L+RLRGLEG A Y F+ M Sbjct: 121 WMLERMTRDYPLRLDVERFKTTSQDMTETMREIQVCENLERLRGLEGTIAVRYNRLFDSM 180 Query: 181 ILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGR 240 ILQQKEDF+F RTRRPPLD VNAMLS AY+LL D ++ALEAVGLDAYVGFLHR+RPGR Sbjct: 181 ILQQKEDFHFKGRTRRPPLDRVNAMLSFAYSLLARDVSAALEAVGLDAYVGFLHRERPGR 240 Query: 241 VSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKK 300 SLALDVMEELRG++AD+FVL+LINKK+I DFL+KENGAVIMT+E RK FL AWQ KK Sbjct: 241 ASLALDVMEELRGIYADRFVLSLINKKVIQGKDFLQKENGAVIMTEEGRKTFLTAWQKKK 300 Query: 301 QETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 QETI HPYL EK+ WGLVP +DL+ YPPFL+K Sbjct: 301 QETIQHPYLKEKMMWGLVPYEQALLLARHLRDDLEAYPPFLYK 343 >C3BU02.1 C3BU02_9BACI CRISPR-associated protein, Cas1 Length = 287 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 219/287 (76%), Positives = 252/287 (87%) Query: 57 MGYCADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKI 116 MGYCA+ NISL+FLTM G++LARV G+S+GNV+LRKKQY +SEDEV SAKIARNFIIGKI Sbjct: 1 MGYCAEKNISLVFLTMYGQFLARVIGKSKGNVILRKKQYRISEDEVMSAKIARNFIIGKI 60 Query: 117 YNHKWMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQ 176 YN+KW++ERMTRDYPLR++V+ FKE SQ LSS+ILEVR CE L+RLRGLEGQAA +Y Sbjct: 61 YNNKWIIERMTRDYPLRIDVDQFKEISQHLSSIILEVRECEDLERLRGLEGQAATSYNKL 120 Query: 177 FNQMILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRD 236 FNQMILQQKEDFYF+ R+RRPPLDNVNAMLS AYTLL +D SALE VGLDAYVGFLHRD Sbjct: 121 FNQMILQQKEDFYFNKRSRRPPLDNVNAMLSFAYTLLANDMTSALEGVGLDAYVGFLHRD 180 Query: 237 RPGRVSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAW 296 RPGRVSLALD MEELRGV+ADKFVL+LINK++++K +FL+KENG+VIMTDEARKKFLAAW Sbjct: 181 RPGRVSLALDTMEELRGVYADKFVLSLINKRVVNKGNFLQKENGSVIMTDEARKKFLAAW 240 Query: 297 QSKKQETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 Q+KK+E +THPYLGE+ISWGLVP NDLDEYPPFLWK Sbjct: 241 QNKKKEKMTHPYLGEQISWGLVPHAQALLLARYLRNDLDEYPPFLWK 287 >C0BZ41.1 C0BZ41_9CLOT Putative uncharacterized protein Length = 343 Score = 466 bits (1198), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/343 (63%), Positives = 271/343 (79%) Query: 1 MKKLLNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYC 60 MKKLLNTLY+T + YL LDG+NVV+L+++E++GR PLHNLE IVTFGYTGASPALMG C Sbjct: 1 MKKLLNTLYVTSTNRYLFLDGENVVILEDQEEIGRVPLHNLEGIVTFGYTGASPALMGAC 60 Query: 61 ADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHK 120 A+ NI L F++ NGR+LARV+G+ RGNV LRK+QY +SE + S KIARNFI GK+YN K Sbjct: 61 AERNIDLSFMSGNGRFLARVSGEVRGNVTLRKEQYRISEQKKESIKIARNFITGKVYNAK 120 Query: 121 WMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQM 180 W+LER RDYPLR++V+ KE S +S + ++R CE +RL G+EG++A+ Y+S F+++ Sbjct: 121 WVLERAARDYPLRLDVDRIKEKSAFMSGNLPKIRECEDAERLLGIEGESASLYFSVFDEL 180 Query: 181 ILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGR 240 ILQQK++F F R +RPPLDNVNAMLS AYTLLT ASALE+VGLD YVGF H DRPGR Sbjct: 181 ILQQKDEFSFGGRNKRPPLDNVNAMLSFAYTLLTGMCASALESVGLDPYVGFYHTDRPGR 240 Query: 241 VSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKK 300 VSLALD+MEELR V AD+FVLTLINKK+I + F KKE+GAVIM D+ R++FL+ WQ KK Sbjct: 241 VSLALDLMEELRSVMADRFVLTLINKKIIGASGFSKKESGAVIMDDDTRRQFLSHWQDKK 300 Query: 301 QETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 +ETITHP+L EK+ WGLVP DLDEYPPFLWK Sbjct: 301 KETITHPFLAEKMEWGLVPYVQAMLLARYIRGDLDEYPPFLWK 343 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,875,517,478 Number of extensions: 71086804 Number of successful extensions: 166327 Number of sequences better than 1.0e-05: 452 Number of HSP's gapped: 165688 Number of HSP's successfully gapped: 453 Length of query: 343 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 207 Effective length of database: 1,779,438,163 Effective search space: 368343699741 Effective search space used: 368343699741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)