BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0002.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7DQI8.1 B7DQI8_9BACL Integrase catalytic region 228 5e-58 C1PSZ1.1 C1PSZ1_ALIAC Transposase 224 8e-57 C2Z078.1 C2Z078_BACCE Transposase, IS21 214 1e-53 Q45618.1 TRA6_BACST Putative transposase for insertion sequence ... 212 6e-53 B9ISY7.1 B9ISY7_BACCQ Transposase (22) 211 8e-53 >B7DQI8.1 B7DQI8_9BACL Integrase catalytic region Length = 413 Score = 228 bits (582), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 133/385 (34%), Positives = 212/385 (55%), Gaps = 12/385 (3%) Query: 1 MRNEEYLTVQEMKQKGMSISRIAQLLGRDRKTIRRWLNGKPPDKQHHLPNL------QKL 54 MR +E + ++++ + G+SIS +A+ G DRKTIR LN +KQ + KL Sbjct: 1 MREDERMEIRQLYEAGVSISELARRFGHDRKTIRNALNSSVEEKQGERASRGERKKGSKL 60 Query: 55 DPYKDYIYQRMNEGCINAVMLLKEIRHKGYQGEATLLREYMRPLRSQIQAKGGNRREALP 114 +PYKDY+ QRM G +NA +L+EIR +GY G T+LRE+M+PLR + AK R E+ P Sbjct: 61 EPYKDYVKQRMQLGVLNAERILREIREQGYTGGITVLREFMKPLRPVVSAKATERYESDP 120 Query: 115 GEEARVDWGEIT-VNLHGIQTKLYLFVMTLEYSQIIYAEFMENQKLETLLNCQLHAMEYF 173 GE+A++D G + HG + ++ F M L YS+++Y EF++ +L +A+E+F Sbjct: 121 GEQAQIDLGAFPYYDSHGQRRTIWAFAMVLAYSRMLYVEFIKAADQLHILQALRNALEFF 180 Query: 174 GGVTRHVIYENLRSVSSGLDSQGRIVWNRRLIQFAGHYHFMMGFYGSRFNSSRG--ERGI 231 GGV R ++ +N + D QG + W + FA Y F+ R + ++G ER I Sbjct: 181 GGVPRVMLSDNCSPLVVANDGQGHVDWQPAYLDFAKFYGFVPKACRPRRSRTKGKVERPI 240 Query: 232 RFVKRNFRVNEYTFTQLGEMNDKVWDWMDDMANKSVHEASAERLVDRLAREELQQINRDR 291 R+++ +F F ++N +V W D +AN +H + E+ + R E+LQ + Sbjct: 241 RYIRDSFW--PVAFVDEEDLNRQVAWWRDHVANVRIHGTTHEQPIVRFQAEKLQPLPATP 298 Query: 292 FEIADHHFRKVSNDCLVSYQANHYSVPGHFAGQVVHILE-QDGCIRVFHGTQEIAVHQKA 350 + +A RKV NDC VS+ N Y+VP + G V + E + G +++ +G Q IA H+ Sbjct: 299 YVLACAGLRKVMNDCRVSWNTNLYTVPWPYVGHTVLVREFESGRLQIEYGGQVIAEHRVL 358 Query: 351 FGKQQIFRQEEHSRTGRKSDSNRVK 375 GK QI EH + + +N + Sbjct: 359 LGKHQISTDPEHYKNIPRDTANPTR 383 >C1PSZ1.1 C1PSZ1_ALIAC Transposase Length = 413 Score = 224 bits (572), Expect = 8e-57, Method: Compositional matrix adjust. Identities = 132/385 (34%), Positives = 212/385 (55%), Gaps = 12/385 (3%) Query: 1 MRNEEYLTVQEMKQKGMSISRIAQLLGRDRKTIRRWLNGKPPDKQHHLPNL------QKL 54 MR +E + ++++ + G+SIS +A+ G DRKTIR LN +KQ + KL Sbjct: 1 MREDERMEIRQLYEAGVSISELARRFGYDRKTIRNALNSSLEEKQGARASRGERKKGSKL 60 Query: 55 DPYKDYIYQRMNEGCINAVMLLKEIRHKGYQGEATLLREYMRPLRSQIQAKGGNRREALP 114 +PYKDY+ QRM G +NA +L+EIR +GY G T+LRE+M+PLR + AK R E+ P Sbjct: 61 EPYKDYVKQRMQLGVLNAERILREIREQGYTGGITVLREFMKPLRPVVSAKATERYESDP 120 Query: 115 GEEARVDWGEIT-VNLHGIQTKLYLFVMTLEYSQIIYAEFMENQKLETLLNCQLHAMEYF 173 GE+A++D G + HG + ++ F M L YS+++Y EF++ +L +A+E+F Sbjct: 121 GEQAQIDLGAFPYYDSHGQRRTIWAFAMVLAYSRMLYVEFIKAADQLHILQALRNALEFF 180 Query: 174 GGVTRHVIYENLRSVSSGLDSQGRIVWNRRLIQFAGHYHFMMGFYGSRFNSSRG--ERGI 231 GGV R ++ +N + D QG + W + FA Y F+ R + ++G ER I Sbjct: 181 GGVPRVMLSDNCSPLVVANDGQGHVDWQPAYLDFAKFYGFVPKACRPRRSRTKGKVERPI 240 Query: 232 RFVKRNFRVNEYTFTQLGEMNDKVWDWMDDMANKSVHEASAERLVDRLAREELQQINRDR 291 R+++ +F F ++N +V W D +AN +H + E+ + R E+LQ + Sbjct: 241 RYIRDSFW--PVAFVDDEDLNRQVAWWRDHVANVRIHGTTHEQPIVRFQAEKLQPLPATP 298 Query: 292 FEIADHHFRKVSNDCLVSYQANHYSVPGHFAGQVVHILE-QDGCIRVFHGTQEIAVHQKA 350 + +A RKV +DC VS+ N Y+VP + G V + E + G +++ +G Q IA H+ Sbjct: 299 YVLACAGLRKVMSDCRVSWNTNLYTVPWRYVGHTVLVREFESGRLQIEYGGQVIAEHRVL 358 Query: 351 FGKQQIFRQEEHSRTGRKSDSNRVK 375 GK QI EH + + +N + Sbjct: 359 SGKHQISTDPEHYKNIPRDSANPTR 383 >C2Z078.1 C2Z078_BACCE Transposase, IS21 Length = 420 Score = 214 bits (545), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 132/367 (35%), Positives = 205/367 (55%), Gaps = 11/367 (2%) Query: 5 EYLTVQEMKQKGMSISRIAQLLGRDRKTIRRWL--NGKPPDKQHHLPNLQKLDPYKDYIY 62 E+ ++E+ QKG + + IA+ G DRKTI ++L N P K KL+PYK YI Sbjct: 6 EFFMIRELFQKGWTKTAIAEATGFDRKTISKYLKSNQLPERKASEQQKESKLEPYKSYIL 65 Query: 63 QRMNEGCINAVMLLKEIRHKGYQGEATLLREYMRPLRSQIQAKGGNRREALPGEEARVDW 122 QR+ EG N V+LL+EI+ GY+G+ T+LR+++RPLR Q + + R E PG++A++DW Sbjct: 66 QRVKEGTTNCVVLLEEIQTMGYEGKMTILRDFVRPLRQQPKKQATLRFETPPGKQAQMDW 125 Query: 123 GEITVNL-HGIQTKLYLFVMTLEYSQIIYAEFMENQKLETLLNCQLHAMEYFGGVTRHVI 181 + L +G +Y FVM L YS++ Y EF N LETL+ C ++A YF GV ++ Sbjct: 126 AYVGKYLVNGNLQDIYAFVMILGYSRMKYVEFTTNMNLETLMKCHMNAFSYFNGVPEQIL 185 Query: 182 YENLRSVSSGLDSQGRIVWNRRLIQFAGHYHFMMGFYGSRFNSSRG--ERGIRFVKRNFR 239 Y+N+++V S I +NR+ F +Y + + ++G ER I+++K NF Sbjct: 186 YDNMKTVVIK-HSPVEIRFNRKFEDFLAYYGIVPKACRPKRPQTKGKVERTIKYLKDNFF 244 Query: 240 VNEYTFTQLGEMNDKVWDWMDDMANKSVHEASAERLVDRLAREE-LQQI--NRDRFEIAD 296 ++ T L +N+ V W+D +ANK ++ + E R +E+ QI + F + Sbjct: 245 QRKHEPT-LHALNEAVRTWLDQIANKKTNQTTNESPFQRFEQEQKFLQIWGVKPLFPTSH 303 Query: 297 HHFRKVSNDCLVSYQANHYSVPGHFAGQVVHILEQ-DGCIRVFHGTQEIAVHQKAFGKQQ 355 R+VS DC+VSY+ YSVP +AGQ+V I E D + ++ + IA H GK Sbjct: 304 WETREVSRDCVVSYRGKRYSVPYRYAGQMVKIKETLDHHLEIYDERECIAKHPVLTGKAT 363 Query: 356 IFRQEEH 362 + EH Sbjct: 364 THIRMEH 370 >Q45618.1 TRA6_BACST Putative transposase for insertion sequence element IS5376 Length = 400 Score = 212 bits (539), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 122/347 (35%), Positives = 203/347 (58%), Gaps = 8/347 (2%) Query: 5 EYLTVQEMKQKGMSISRIAQLLGRDRKTIRRWLNGK-PPDKQHHLPNLQKLDPYKDYIYQ 63 E+ ++EM ++GMSIS IA+ LG DRKT+R++++ PP K KLDP+K Y+ + Sbjct: 6 EFFMIKEMYERGMSISDIARELGIDRKTVRKYIHSPNPPSKSKRKQRKSKLDPFKPYLQK 65 Query: 64 RMNE-GCINAVMLLKEIRHKGYQGEATLLREYMRPLRSQIQAKGGNRREALPGEEARVDW 122 RM E G N+ L EIR +GY G T+L++YM+P R + K R E LPGE+ +VDW Sbjct: 66 RMLEDGVFNSEKLFFEIRQQGYTGGKTILKDYMKPFRETAKKKYTVRYETLPGEQMQVDW 125 Query: 123 GEI-TVNLHGIQTKLYLFVMTLEYSQIIYAEFMENQKLETLLNCQLHAMEYFGGVTRHVI 181 E+ V + G + KL LFV TL YS++ YA F +Q E L+ C + + +YFGGV + V+ Sbjct: 126 KEVGEVVIEGKKVKLSLFVATLGYSRMKYAVFTTSQDQEHLMECLIQSFKYFGGVPKKVL 185 Query: 182 YENLRSVSSGLDSQGRIVWNRRLIQFAGHYHFMMGFYGSRFNSSRG--ERGIRFVKRNFR 239 ++N+++V+ G + QG + WN+R +FA +Y F+ ++G ER I+++ +F Sbjct: 186 FDNMKTVTDGRE-QGVVKWNQRFSEFASYYGFIPKVCRPYRAQTKGKVERAIQYIMDHFY 244 Query: 240 VNEYTFTQLGEMNDKVWDWMDDMANKSVHEASAERLVDRLAREELQQINRDRFEIADHHF 299 V F + E+N + W+D +AN+ + + +R A E L+ + ++ + + Sbjct: 245 VGT-AFESIEELNFLLHRWLDQVANRKPNATTGISPQERWAEESLKPLPLKDYDTSYLSY 303 Query: 300 RKVSNDCLVSYQANHYSVPGHFAGQVVHILEQ-DGCIRVFHGTQEIA 345 RKV D SY+ + + +AG+ + + E+ +G IR++ +EI+ Sbjct: 304 RKVHWDGSFSYKGEQWLLSAEYAGKEILVKERLNGDIRLYFRGEEIS 350 >B9ISY7.1 B9ISY7_BACCQ Transposase (22) Length = 412 Score = 211 bits (538), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 131/363 (36%), Positives = 203/363 (55%), Gaps = 11/363 (3%) Query: 9 VQEMKQKGMSISRIAQLLGRDRKTIRRWL--NGKPPDKQHHLPNLQKLDPYKDYIYQRMN 66 ++E+ QKG + + IA+ G DRKTI ++L N P K KL+PYK YI QR+ Sbjct: 2 IRELFQKGWTKTAIAEATGFDRKTISKYLKSNQLPERKASEQQKESKLEPYKSYILQRVK 61 Query: 67 EGCINAVMLLKEIRHKGYQGEATLLREYMRPLRSQIQAKGGNRREALPGEEARVDWGEIT 126 EG N V+LL+EI+ GY+G+ T+LR+++RPLR Q + + R E PG++A++DW + Sbjct: 62 EGTTNCVVLLEEIQTMGYEGKMTILRDFVRPLRQQPKKQATLRFETPPGKQAQMDWAYVG 121 Query: 127 VNL-HGIQTKLYLFVMTLEYSQIIYAEFMENQKLETLLNCQLHAMEYFGGVTRHVIYENL 185 L +G +Y FVM L YS++ Y EF N LETL+ C ++A YF GV ++Y+N+ Sbjct: 122 KYLVNGNLQDIYAFVMILGYSRMKYVEFTTNMNLETLMKCHMNAFSYFNGVPEQILYDNM 181 Query: 186 RSVSSGLDSQGRIVWNRRLIQFAGHYHFMMGFYGSRFNSSRG--ERGIRFVKRNFRVNEY 243 ++V S I +NR+ F +Y + + ++G ER I+++K NF ++ Sbjct: 182 KTVVIK-HSPVEIRFNRKFEDFLAYYGIVPKACRPKRPQTKGKVERTIKYLKDNFFQRKH 240 Query: 244 TFTQLGEMNDKVWDWMDDMANKSVHEASAERLVDRLAREE-LQQI--NRDRFEIADHHFR 300 T L +N+ V W+D +ANK ++ + E R +E+ QI + F + R Sbjct: 241 EPT-LHALNEAVRTWLDQIANKKTNQTTNESPFQRFEQEQKFLQIWGVKPLFPTSHWETR 299 Query: 301 KVSNDCLVSYQANHYSVPGHFAGQVVHILEQ-DGCIRVFHGTQEIAVHQKAFGKQQIFRQ 359 +VS DC+VSY+ YSVP +AGQ+V I E D + ++ + IA H GK + Sbjct: 300 EVSRDCVVSYRGKRYSVPYRYAGQMVKIKETLDHHLEIYDERECIAKHPVLTGKATTHIR 359 Query: 360 EEH 362 EH Sbjct: 360 MEH 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,198,348,563 Number of extensions: 89229608 Number of successful extensions: 211672 Number of sequences better than 1.0e-05: 984 Number of HSP's gapped: 210057 Number of HSP's successfully gapped: 985 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)