BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0492.0005.p (309 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9RC63.1 Q9RC63_BACHD Putative uncharacterized protein 278 6e-73 C1TQB8.1 C1TQB8_9BACT CRISPR-associated protein, Cmr4 family 196 2e-48 C5BIW5.1 C5BIW5_9GAMM CRISPR-associated ramp protein, Cmr4 family 193 2e-47 B1I5K7.1 B1I5K7_DESAP CRISPR-associated RAMP protein, Cmr4 family 181 9e-44 A9AWU4.1 A9AWU4_HERA2 CRISPR-associated RAMP protein, Cmr4 family 181 1e-43 >Q9RC63.1 Q9RC63_BACHD Putative uncharacterized protein Length = 303 Score = 278 bits (710), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 145/315 (46%), Positives = 201/315 (63%), Gaps = 25/315 (7%) Query: 1 MTNNRLYYVHCMSSVHIGTGQGVGIIDMPMIREKVTEWPYLPGSSMKGVHRVFFKSGIHK 60 + +++L++VHC+S VHIGTG+GVG+IDMP++RE+VT+WP +PGS+ KGVHR +F + Sbjct: 3 LNSSQLFFVHCLSPVHIGTGEGVGLIDMPIMRERVTKWPLIPGSTTKGVHRDYFVQ--QR 60 Query: 61 QPEKWLNSAFGKASNKGTNFNSDDGFELDDGNAGALVMSDAKILAFPVASRYGTFAYVTC 120 E W+ +AFG + +GNAG++VMSDA+ILAFPVAS YGTFAYVTC Sbjct: 61 DKESWIRTAFGAGKGEADA----------EGNAGSIVMSDARILAFPVASYYGTFAYVTC 110 Query: 121 PLVLKRFRRDTVAAGVD-----MPEFD-WAALESVVNSGVVMLHTDSKLDKNNE-VFVDE 173 P+V+KR RD AA V + + + W S ++ H D+NNE +++DE Sbjct: 111 PMVIKRLERDMGAANVSYFSNLIEKVEGWEKKLSTNEDKALIGHRCEVTDENNEKIYLDE 170 Query: 174 FTSGAVKDEAFAKWTDWLAGQIFVKDELSETMLKERMLLVSDEAFQYFVSMCSEVVPRIR 233 F V D F + T+ LA +F D S+ +L R++LVSDE+FQYFV+ CSE+ PRIR Sbjct: 171 FELSVVIDGTFGELTNSLAKLVFKDDHFSQELLASRLVLVSDESFQYFVTQCSEITPRIR 230 Query: 234 IGLETGSVEPGALWNEEYLPVESILYGVIWSDGI-SVKTLENRGLLDIFPEEAFLQIGGN 292 + E V+ GALW EEYLP E++LYG+IW + + VK R + LQ+GGN Sbjct: 231 LKAEEKVVDDGALWYEEYLPTETLLYGLIWCEKMDQVKESWKR-----LSKGRVLQVGGN 285 Query: 293 ATVGKGRIRCRYVKG 307 ATVG+GR+R Y G Sbjct: 286 ATVGRGRVRYLYTGG 300 >C1TQB8.1 C1TQB8_9BACT CRISPR-associated protein, Cmr4 family Length = 294 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 112/307 (36%), Positives = 170/307 (55%), Gaps = 25/307 (8%) Query: 3 NNRLYYVHCMSSVHIGTGQGVGIIDMPMIREKVTEWPYLPGSSMKGV----HRVFFKSGI 58 ++ Y++H +S +H+G+G+GVG ID+P+ REKVT WPY+PGS++KGV H G Sbjct: 5 TSKSYWIHALSPLHVGSGRGVGHIDLPIAREKVTNWPYIPGSALKGVIADHHDASAGKGR 64 Query: 59 HKQPEKWLNSAFGKASNKGTNFNSDDGFELDDGNAGALVMSDAKILAFPVASRYGTFAYV 118 +K +AFG A + D NAGALV SDA+I+ PV S YGTFA+ Sbjct: 65 EDCAKK--KAAFGTAGD-------------DSSNAGALVFSDARIVCLPVQSLYGTFAWC 109 Query: 119 TCPLVLKRFRRDTVAAGVDMPEFDWAALESVVNSGVVMLHTDSKLDKNNEVFVDEFTSGA 178 T P+VL++ RD + + +L SG L + + ++ +++ + Sbjct: 110 TSPMVLRQLIRDLTGIVPGLEDLKVPSL-----SGEKALASKKTVLGDDTLYLQDLDVPM 164 Query: 179 VKDEAFAKWTDWLAGQIFVKDELSETMLKERMLLVSDEAFQYFVSMCSEVVPRIRIGLET 238 + KW ++LAGQ+F +DE +R +++SDE F Y M ++V RIRI E Sbjct: 165 EGCDYVGKWAEFLAGQVFPEDEGWRGEFDKRFVVLSDEVFNYLSEMGTQVDARIRIDPEN 224 Query: 239 GSVEPGALWNEEYLPVESILYGVIWSDGISVKTLENRGLLDIFPE-EAFLQIGGNATVGK 297 G V G LW EE LP E++L G++W D ++VK + L+ F + LQ+GG ATVG+ Sbjct: 225 GVVATGGLWYEEALPAEAVLAGMVWCDKVTVKGVSEDELVKGFCSGKLCLQVGGKATVGR 284 Query: 298 GRIRCRY 304 GR+ + Sbjct: 285 GRVSLSF 291 >C5BIW5.1 C5BIW5_9GAMM CRISPR-associated ramp protein, Cmr4 family Length = 298 Score = 193 bits (490), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 104/306 (33%), Positives = 166/306 (54%), Gaps = 25/306 (8%) Query: 1 MTNNRLYYVHCMSSVHIGTGQGVGIIDMPMIREKVTEWPYLPGSSMKGVHRVFFKSGIHK 60 + ++RLY++H ++ VH+G G+G+ ID+P++REK+T WP +P S++KGV R ++ + Sbjct: 5 LKHSRLYWLHALTPVHVGVGRGLDYIDLPIMREKITHWPIVPSSAVKGVLRAHYE----E 60 Query: 61 QPEKWLNSAFGKASNKGTNFNSDDGFELDDGNAGALVMSDAKILAFPVASRYGTFAYVTC 120 ++ AFG + D NAG+L +DA I+ PV S YGTFA+ T Sbjct: 61 TENSLVDVAFGSGGDS-------------DANAGSLNFTDAHIVCLPVRSLYGTFAWCTS 107 Query: 121 PLVLKRFRRDTVAAGVDMPEFDWAALESVVNSGVVMLHTDSKLDKNNEVFVDEFTSGAVK 180 P+ L+RF RD G D + + +V N+ + +D+ L + N VF+++ Sbjct: 108 PMALRRFLRDITTYGGDGAGLE---IPAVANANASITSSDTALAERNTVFLEDLDFSNTA 164 Query: 181 DEAFAKWTDWLAGQIFVKDELSETMLKERMLLVSDEAFQYFVSMCSEVVPRIRIGLETGS 240 E W ++A +F + E + R +++SD+ F + +EV RIRI ET + Sbjct: 165 SEQAKNWALYIARNVFGESSWQEAFVS-RFIILSDDTFNFLCDTGTEVNARIRIEQETKT 223 Query: 241 VEPGALWNEEYLPVESILYGVIWSD----GISVKTLENRGLLDIFPEEAFLQIGGNATVG 296 V PGALW EE LP E++L G +W + G + T + + LQIGGNAT G Sbjct: 224 VVPGALWYEESLPAETLLAGGVWCERLYKGRAGSTTADELFSQYCCKPLHLQIGGNATTG 283 Query: 297 KGRIRC 302 +G++RC Sbjct: 284 QGQVRC 289 >B1I5K7.1 B1I5K7_DESAP CRISPR-associated RAMP protein, Cmr4 family Length = 288 Score = 181 bits (458), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 108/312 (34%), Positives = 168/312 (53%), Gaps = 39/312 (12%) Query: 5 RLYYVHCMSSVHIGTGQGVGIIDMPMIREKVTEWPYLPGSSMKGVHRVFFKSGIHKQPEK 64 RL +V+ +SS+H GT QGVG+ID+P+ REK T PYLPGSS+KGV R + ++ Sbjct: 6 RLLFVYALSSLHAGTEQGVGVIDLPVAREKATGLPYLPGSSLKGVLRDACRDNEMRK--- 62 Query: 65 WLNSAFGKASNKGTNFNSDDGFELDDGNAGALVMSDAKILAFPVASRYGTFAYVTCPLVL 124 FG ++D E +AG++ +SD ++L PV S GTFA+VT P +L Sbjct: 63 ---KVFGP--------DADSAHE----HAGSVQISDQRLLLLPVRSLKGTFAWVTSPYIL 107 Query: 125 KRFRRD-------TVAAGVDMPEFDWAALESVVNSGVVMLHTDSKLDKNNEVFVDEFTSG 177 +RF RD ++ +GV +P + + L S S +V+ NN+V +++ Sbjct: 108 RRFLRDAENVGVSSIPSGVPVPTLETSCLVSNDGSAIVL---------NNQVILEDLDLT 158 Query: 178 AVKDEAFAKWTDWLAGQIFVKDELSETMLKERMLLVSDEAFQYFVSMCSEVVPRIRIGLE 237 A + +W +W+ Q+F D + ML R +V D+ + + +EV R+R+ + Sbjct: 159 AETNSDAREWAEWIDRQVFPDDTSGQEMLNARFCVVHDDVMSFLLDTATEVFARVRLRED 218 Query: 238 TGSVEPGALWNEEYLPVESILYGVIWSDGISVKTLENRGLLDIFP--EEAFLQIGGNATV 295 +VE G LW EE LP E++L G+I ++ + K E + + F E LQ+GG ATV Sbjct: 219 AKTVETGGLWYEEALPAETVLAGLILANPVKAKPDE---VFETFSGLMEKPLQLGGKATV 275 Query: 296 GKGRIRCRYVKG 307 G+G R R + G Sbjct: 276 GRGLCRLRMIGG 287 >A9AWU4.1 A9AWU4_HERA2 CRISPR-associated RAMP protein, Cmr4 family Length = 293 Score = 181 bits (458), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 111/311 (35%), Positives = 165/311 (53%), Gaps = 35/311 (11%) Query: 3 NNRLYYVHCMSSVHIGTGQGVGIIDMPMIREKVTEWPYLPGSSMKGVHRVFFKSGIHKQP 62 +L +VH +S +H GTGQG G+ID+P+ REK T PYLPGSS+KG R + +G K Sbjct: 2 TTKLVFVHALSPLHAGTGQGAGVIDLPIAREKATNLPYLPGSSLKGPLRDGY-TGARK-- 58 Query: 63 EKWLNSAFGKASNKGTNFNSDDGFELDDGNAGALVMSDAKILAFPVASRYGTFAYVTCPL 122 + FG AS+ T GNAG + SD ++L PV S YGTFA+VT P Sbjct: 59 ----DEIFGTASDNAT------------GNAGFVQFSDLRLLCMPVRSLYGTFAWVTSPY 102 Query: 123 VLKRFRRD--------TVAAGVDMPEFDWAALESVVNSGVVMLHTDSKLDKNNEVFVDE- 173 +L R R+ T + ++ P+ D + V +G + K K ++++++ Sbjct: 103 ILNRLAREIKNLFTENTPPSWIENPDVD----QCFVVAGSALKSNLPKSAKRKDIYLEDL 158 Query: 174 -FTSGAVKDEAFAKWTDWLAGQIFVKDELSETMLKERMLLVSDEAFQYFVSMCSEVVPRI 232 F S A + E +W DWL +IF +TM ER +V+D+ F + ++ +E+ RI Sbjct: 159 DFESQADQAELVTQWADWLKKRIFGDSPEWQTMFVERFCIVNDDVFSFLLNTATEITARI 218 Query: 233 RIGLETGSVEPGALWNEEYLPVESILYGVIWSDGISVKTLENRGLLDIFPE--EAFLQIG 290 R+ ET +V G LW EE LP ESIL G++ + KT + + P + +Q+G Sbjct: 219 RLNDETKTVADGQLWYEEALPAESILAGLMLIAPPASKTDLIQSITTELPRLWQKPIQLG 278 Query: 291 GNATVGKGRIR 301 G ATVG+G R Sbjct: 279 GKATVGRGICR 289 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,972,225,538 Number of extensions: 84121332 Number of successful extensions: 190212 Number of sequences better than 1.0e-05: 75 Number of HSP's gapped: 190035 Number of HSP's successfully gapped: 76 Length of query: 309 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 174 Effective length of database: 1,788,859,178 Effective search space: 311261496972 Effective search space used: 311261496972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)