BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0492.0004.p (419 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KFY7.1 Q9KFY7_BACHD BH0329 protein 357 1e-96 Q9RC64.1 Q9RC64_BACHD Putative uncharacterized protein 256 2e-66 C5BIW6.1 C5BIW6_9GAMM CRISPR-associated protein, Cmr3 family 186 3e-45 C1TQB7.1 C1TQB7_9BACT CRISPR-associated protein, Cmr3 family 171 1e-40 A5D0Z3.1 A5D0Z3_PELTS Uncharacterized protein 137 3e-30 >Q9KFY7.1 Q9KFY7_BACHD BH0329 protein Length = 423 Score = 357 bits (916), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 182/418 (43%), Positives = 260/418 (62%), Gaps = 36/418 (8%) Query: 1 MIRDGRPFGNTPGARAHTLGTVTPGVVAGTLRTLMQKNNTAGTFGTTTKDAEREGKPSTV 60 M+RDGRPF ++PGARA+++ V PGV+AGT+RT++ + + R T Sbjct: 17 MVRDGRPFKDSPGARAYSMMDVVPGVIAGTVRTMLAEQAAKSS---------RVVDYHTF 67 Query: 61 AKVDIRGPLYMLNDRLFLPMPYDLDLYEPGLQEDPASIHVNVRRPIEPAKNTPTHLHGFL 120 +KV + GPLY+ + F P+P D+ YE ++ ++ +N R+P +P N G+L Sbjct: 68 SKVPVYGPLYVWKGKPFFPLPSDIGFYE----DENGALQMNYRQP-QPLVNG----QGYL 118 Query: 121 GTGKTSLHEDKLWPVSMDTEIRKPLKSAPAYISAEWMIKWLCDNLQPEQWSAAIAEWRSK 180 G G HE LWP + ++ K PAY+S +WM +WL +L P+ W++ +AEW+ Sbjct: 119 GIGDKGDHE-SLWPAKVIPQMGKVSSIVPAYLSLDWMSRWLRGDLSPKSWTSPLAEWQQL 177 Query: 181 PEDK--------VHFIAPFVKEVRTHTAIQAETSTVQDKALFSTESLVIPPDVSLIASAS 232 D VHF+ PF + R H A++A T +D+ALF+T+S+V PPD+ + A Sbjct: 178 VRDNKLDSTDKDVHFLPPFALDERDHIAVEAGTRMAKDQALFTTQSIVFPPDLDMHARVE 237 Query: 233 IGSDVKWEGTISGIHSFGGKRRLAHFREIDDQEIQDNIWKCPEAIAQAVAENPRYLRMVL 292 I + W +S +HS GGKRRLAHF E D D W+CPEA+ Q++ E+ +Y+R+VL Sbjct: 238 IPQEAAWPMQLSTLHSLGGKRRLAHFSESQD----DQFWQCPEALEQSLDES-QYVRLVL 292 Query: 293 ATSAYFSKGWLPGWLDGDLRSTDRFDKQL----QIELCWACLPRWEGISGWSYRHNQPKA 348 AT AYF+KGW P WLD +L + + F K++ +++L WAC+ RW+ +SGWSY QPKA Sbjct: 293 ATPAYFAKGWKPRWLDDELCTNEIFAKEISREVKLQLVWACISRWQPVSGWSYSLKQPKA 352 Query: 349 VRRMVAAGSVYFFKVISGDPVEFVRRNWLTSMSDEDRRKAFFDKQDGYGLATWGVWTP 406 VRRMV AGSVYFFKV G+P E RR WL ++SD +RRK FDK+DG+GLA WG W P Sbjct: 353 VRRMVPAGSVYFFKVTEGNPRELARRGWLNTVSDANRRKGAFDKEDGFGLALWGTWRP 410 >Q9RC64.1 Q9RC64_BACHD Putative uncharacterized protein Length = 262 Score = 256 bits (655), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 123/254 (48%), Positives = 169/254 (66%), Gaps = 17/254 (6%) Query: 165 LQPEQWSAAIAEWRSKPEDK--------VHFIAPFVKEVRTHTAIQAETSTVQDKALFST 216 + P+ W++ +AEW+ D VHF+ PF + R H A++A T +D+ALF+T Sbjct: 1 MSPKSWTSPLAEWQQLVRDNKLDSTDKDVHFLPPFALDERDHIAVEAGTRMAKDQALFTT 60 Query: 217 ESLVIPPDVSLIASASIGSDVKWEGTISGIHSFGGKRRLAHFREIDDQEIQDNIWKCPEA 276 +S+V PPD+ + A I + W +S +HS GGKRRLAHF E D D W+CPEA Sbjct: 61 QSIVFPPDLDMHARVEIPQEAAWPMQLSTLHSLGGKRRLAHFSESQD----DQFWQCPEA 116 Query: 277 IAQAVAENPRYLRMVLATSAYFSKGWLPGWLDGDLRSTDRFDKQL----QIELCWACLPR 332 + Q++ E+ +Y+R+VLAT AYF+KGW P WLD +L + + F K++ +++L WAC+ R Sbjct: 117 LEQSLDES-QYVRLVLATPAYFAKGWKPRWLDDELCTNEIFAKEISREVKLQLVWACISR 175 Query: 333 WEGISGWSYRHNQPKAVRRMVAAGSVYFFKVISGDPVEFVRRNWLTSMSDEDRRKAFFDK 392 W+ +SGWSY QPKAVRRMV AGSVYFFKV G+P E RR WL ++SD +RRK FDK Sbjct: 176 WQPVSGWSYSLKQPKAVRRMVPAGSVYFFKVTEGNPRELARRGWLNTVSDANRRKGAFDK 235 Query: 393 QDGYGLATWGVWTP 406 +DG+GLA WG W P Sbjct: 236 EDGFGLALWGTWRP 249 >C5BIW6.1 C5BIW6_9GAMM CRISPR-associated protein, Cmr3 family Length = 395 Score = 186 bits (473), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 143/418 (34%), Positives = 201/418 (48%), Gaps = 56/418 (13%) Query: 1 MIRDGRPFGNTPGARAHTLGTVTPGVVAGTLRTLMQKNNTAGTFGTTTKDAEREGKPSTV 60 + RDGRPFG + G R +L P + +G+LR+L+ K G F T T A + Sbjct: 15 IARDGRPFGASSGNRMRSLPWFYPSLFSGSLRSLLGK--ATGGFSTETVAA--------L 64 Query: 61 AKVDIRGPLYMLNDRLFLPMPYDLDLYEPGLQEDPASIHVNVRRPIEPAKNTPTHLHGFL 120 ++I GPL +LN+ LF P P D ++E G A H RP G L Sbjct: 65 KALEIAGPLALLNNTLFFPRPLDARVFESG-----AERHCYGLRP------------GAL 107 Query: 121 GTGK-TSLHEDKLWPVSMDTEIRKPLKSAPAYISAEWMIKWLCDNLQPEQWSAAIAEWRS 179 G G+ L L P + + PA I+ W + D L S A + Sbjct: 108 GAGEGVDLPGPNLLPTLLPDGAN--MDGKPAEIAQFWSSARMADWLTRSSASGFTAPPDN 165 Query: 180 KP--EDKVHFIAPFVKEVRTHTAIQAETSTVQDKALFSTESL--VIPPDVSLIASASIGS 235 P D + AP V + RTHTA+ E D LF T L +P ++ S Sbjct: 166 TPTSADTGYLAAPPV-DFRTHTALSDEKGVADDGKLFITAGLDLNLPNTADIVQLVSRVG 224 Query: 236 DVKW-----EGTISGIHSFGGKRRLAHFREIDDQEIQDNIWKCPEAIAQAVAENPRYLRM 290 + W EG ++ +H GG RRLA+++ DDQ ++W+CP+ +A A+A+ + +R+ Sbjct: 225 NSGWCQQVLEG-LAQVHPMGGDRRLANWQHTDDQ----HLWECPQGVASALAKATK-IRL 278 Query: 291 VLATSAYFSKGWLPGWLDGDLRST--DRFDKQLQIELCWACLPRWEGISGWSYRHNQ--P 346 LAT F GWLPGWLD + + L++ L C RW+ +SGWS + P Sbjct: 279 YLATPGLFDGGWLPGWLDKTTFTGCPPGIEGGLKLTLRAVCSERWQPLSGWSLERDSWGP 338 Query: 347 KAVRRMVAAGSVYFFKVISGDPVEFVRRNWLTSMSDEDRRKAFFDKQDGYGLATWGVW 404 KA RRMV AGSVYFF GD + V R WL+S+ D+ + D+ DG+GLA WG W Sbjct: 339 KAARRMVPAGSVYFFTCDQGDAAQLVPR-WLSSVCDKAQ-----DRNDGFGLALWGTW 390 >C1TQB7.1 C1TQB7_9BACT CRISPR-associated protein, Cmr3 family Length = 394 Score = 171 bits (434), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 141/418 (33%), Positives = 201/418 (48%), Gaps = 52/418 (12%) Query: 1 MIRDGRPFGNTPGARAHTLGTVTPGVVAGTLRTLMQKNNTAGTFGTTTKDAEREGKPSTV 60 + RDGRPF + G R H+L P VAG+LRT++ K G+F D + Sbjct: 14 IARDGRPF--SFGNRMHSLDWPKPSTVAGSLRTMIGKL-AGGSFDKKMVDG--------L 62 Query: 61 AKVDIRGPLYMLN----DRLFLPMPYDLDLYEPGLQEDPASIHVNVRRPIEPAKNTPTHL 116 + I GPL + + D LF P P D+ + + E + RR +E + Sbjct: 63 KAMTILGPLPLESSSNGDFLFFPAPKDILVQKDNAAEKKGPFMLRPRR-VEDRCGS---- 117 Query: 117 HGFLGTGKTSLHEDKLWPVSMDTEIRKPLKSA--PAYISAEWMIKWLCDNLQPEQWSAAI 174 + T L E++ P M+ E + K P + ++ MIKWL Q A++ Sbjct: 118 -----SCGTDLGEEEYLPF-MEPEPEESFKPGKIPPFWRSDLMIKWLL-----AQDVASM 166 Query: 175 AEWRSKPED-KVHFIAPFV----KEVRTHTAIQAETSTVQDKALFSTESLVI-PPDVSLI 228 + K E+ K +PF KE R H +I ++ T +D LFST L + P +S+ Sbjct: 167 PKIDPKDENYKAKGSSPFKDFPRKEDRVHVSIDSQKGTAEDGMLFSTTGLDMREPGMSMS 226 Query: 229 ASASIGSDVKWEGTISGIHSFGGKRRLAHFREIDDQEIQDNIWKCPEAIAQAVA--ENPR 286 + + G +S H GG+RRLA + D+ + +W CP+ I A+ Sbjct: 227 LRVKCETYGEIIGDLSEHHPLGGERRLARW----DRSKDEALWSCPDEILDALKGLRPGD 282 Query: 287 YLRMVLATSAYFSKGWLPGWLDGDLRSTDRFDKQLQIELCWACLPRWEGISGWSYRHNQP 346 +RMVL TSA F KGWLPGWLD D + +++ L A RWE +SGWSY +P Sbjct: 283 GIRMVLGTSAVFEKGWLPGWLDDDKKGKIP-GTSVEVRLISAVTSRWEPLSGWSYETKRP 341 Query: 347 KAVRRMVAAGSVYFFKVISGDPVEFVRRNWLTSMSDEDRRKAFFDKQDGYGLATWGVW 404 K + RMV AGSVYF +++S D E WL +SDE K DGYGLA WG+W Sbjct: 342 KPLSRMVPAGSVYFLELLSEDASELA-EVWLEPVSDEK-----LYKNDGYGLALWGLW 393 >A5D0Z3.1 A5D0Z3_PELTS Uncharacterized protein Length = 396 Score = 137 bits (344), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 136/427 (31%), Positives = 192/427 (44%), Gaps = 75/427 (17%) Query: 1 MIRDGRPFGNTPGARAHTLGTVTPGVVAGTLRTLMQKNNTAGTFGTTTKDAEREGKPSTV 60 + RDGRPFG PGARA TL P +AG +RT + +G F + + + Sbjct: 13 IARDGRPFGPVPGARAFTLAFPFPSTIAGAVRT-RDGLDASGRF--------QPAEIPRL 63 Query: 61 AKVDIRGPLYM-LN------DRLFLPMPYDLDLYEPGLQEDPASIHVNVRRPIEPAKNTP 113 K+++ GPL + L+ DR +P P D +E P+ R + P + P Sbjct: 64 KKIEVLGPLLVELDAGTGDIDRWLVPAPADALFFE----LVPSDFTKAAIRQLTPLQLPP 119 Query: 114 THLHGFLGTGKTSLHEDKLWPVSM-DTEIRKPLKSAPAYISAEWMIKWLCDNLQPEQWSA 172 T+L ED L PV M D + RKP PAY + KWL D P Sbjct: 120 GS--------HTNLPEDSLAPVGMPDRDPRKPFSKTPAYWYWKLFEKWLVD---PFDGDV 168 Query: 173 AIAEWRSKPEDKVHFIAPFVKEVRTHTAIQAETSTVQDK--ALFSTESLVIPPDVSLIAS 230 + E V P V+EVR H +I+ +T T ++ ALF T L V A Sbjct: 169 QLVE--------VGHNGP-VQEVRMHASIRPDTQTADEEKGALFQTRGLEFACPVEDHAD 219 Query: 231 ASIGSDVKWEGTIS---------GIHSFGGKRRLAHFREIDDQEIQDNIWKCPEAIAQAV 281 I S V+ G + GI GG+RRLA +R+ + + CPEA+ + + Sbjct: 220 GGILSRVRRLGLAAAADAPNLKPGIAPLGGERRLAFWRQSNQV-----LPSCPEALKEKI 274 Query: 282 AENPRYLRMVLATSAYFSKGWLPGWLDGDLRSTDRFDKQLQIELCWACLPRWEGISGWSY 341 A + R++L T A+F GW P L L + LQ A L R + +SGW Sbjct: 275 AAL-GHCRLILLTPAHFGAGWKPSRL---LEEWEGVRPYLQA----AALKRHQTVSGWDL 326 Query: 342 R-HNQPKAVRRMVAAGSVYFFKVISGDPVEFVR---RNWLTSMSDEDRRKAFFDKQDGYG 397 + K RR+ AG+VYF K ++GD R WLT +SD ++ D++DG+G Sbjct: 327 EGKGRRKPTRRLAPAGTVYFLK-LNGDSAAIKRWVDSIWLTCVSDGEQ-----DRRDGFG 380 Query: 398 LATWGVW 404 LA GVW Sbjct: 381 LAVTGVW 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,810,631,456 Number of extensions: 121300988 Number of successful extensions: 269965 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 269900 Number of HSP's successfully gapped: 21 Length of query: 419 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 281 Effective length of database: 1,760,596,133 Effective search space: 494727513373 Effective search space used: 494727513373 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)