BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0492.0003.p (604 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9KFY8.1 Q9KFY8_BACHD BH0328 protein 471 e-130 C1TQB6.1 C1TQB6_9BACT CRISPR-associated protein, Cmr2 family 244 3e-62 C0DRI5.1 C0DRI5_NEICI Putative uncharacterized protein 200 4e-49 A5D0V5.1 A5D0V5_PELTS Predicted hydrolase 195 1e-47 B1I5L0.1 B1I5L0_DESAP CRISPR-associated protein, Crm2 family 190 3e-46 >Q9KFY8.1 Q9KFY8_BACHD BH0328 protein Length = 604 Score = 471 bits (1211), Expect = e-130, Method: Compositional matrix adjust. Identities = 268/610 (43%), Positives = 367/610 (60%), Gaps = 22/610 (3%) Query: 1 MLISIGPVQEFIAQARKTRDLWFGSHLLSEISKEAARRLA--EAHNARMIFPYIHK-EKI 57 ML SIGPVQEFIAQARKTRDLWFGSHLLSE+ K AA +L+ EAH +IFPY+ EK+ Sbjct: 11 MLFSIGPVQEFIAQARKTRDLWFGSHLLSELGKAAAFKLSKLEAH---VIFPYVENLEKL 67 Query: 58 -AYGLDDLKVANKVLAIVPTNDPKAVALDVRRAVVTKWLGYANKAKEELGSGIIGPMWER 116 A + + N+V+A+V T++PKAVAL+V+ AV+ KW+ YAN A+ LG I +W+R Sbjct: 68 DAKEKEKINSPNRVMALVDTSEPKAVALEVKAAVIEKWMSYANGARRLLGRDINPALWKR 127 Query: 117 QVKDFIEFYAVWSELESEEKYAEVLQQTEQLMAARKTLRDFRPNEPADLFGDDKSGLDSG 176 QVKD IEF+AVW + + E YAEV Q+ E+L+AARKTLRDF+PN+P+ ++G+ KS LD G Sbjct: 128 QVKDLIEFHAVWMDCKEEAHYAEVRQKAEELLAARKTLRDFKPNQPSHMYGEVKSSLDPG 187 Query: 177 RESVLKPSQYMSYAGFGIKHKETLDAISLVKRLS-RFTVDKKEFKSVCDVAFQQFRDELG 235 RE VL+ QY YA +GIK E+LDAISLVKRLS + + + F SVCDVAF Q R ++ Sbjct: 188 RECVLEKHQYKRYAPYGIKKDESLDAISLVKRLSFHLSPNTRHFPSVCDVAFSQLRRKI- 246 Query: 236 ASRNQWMKTAVDEYYRDLKHKYGTALKLTGSDLQSYKSEMFYTSRXXXXXXXXXXXMKKP 295 ++ + Y + +KY K SDL + S +FY + Sbjct: 247 -EKDNLLTDLAKNYQEWVWNKYPKLKKRLFSDL--FDSRLFYVTHIKEMVEGAYP----- 298 Query: 296 INRVKQLEITQNILTSLEELYGKILKEPTPYYALVVGDGDRMGDALRKMKTPAEHQSFTE 355 N+ +Q +I + I LE Y + TPYYA +V DGD+MG ALR +K +HQ+F++ Sbjct: 299 -NKNEQEQIIKLIAQKLESFYKETKLNSTPYYAFLVCDGDQMGKALRNLKNIEDHQAFSK 357 Query: 356 HLSGFASEVERIVFKDDI-KGQMIYSGGDDLMALLPVHRCLAAAQEIRQAFTNTMTLALP 414 LS FA++ +IV D +G+++Y+GGDD+MA LP+HRCL A +++Q F M ALP Sbjct: 358 KLSEFAAKARKIVTTDKRDEGELVYAGGDDVMAYLPLHRCLDVAAKLQQLFGELMNEALP 417 Query: 415 DEDQRPTLSVGIAIVHMFEPLEDSLLIARAAEALAKVRRDELAIHFQKRSGSEEMRVSLP 474 E RPTLSVGI I HM EP+ + +A AAE AK +R+ELAI FQKRSG + MR S+ Sbjct: 418 KEAVRPTLSVGIVIAHMMEPMSEVRKLAMAAEKQAKRKRNELAIFFQKRSGGDRMRFSVS 477 Query: 475 FSENPVNTIVKLQSLYRNRYISTSFAYGLRQLHAEYLDLKAHGSLQNEGMGXXXXXXXXX 534 F P I K+ Y +S A+ ++ LH EY L + + +E Sbjct: 478 FDTEPTRNIKKILKFYYRNVMSVGIAHAIQSLHTEYSGLLQNQLVMSEERLEKLLEQEVS 537 Query: 535 XXXXKKPEFKSKDEIIKEVLSVFKGLEQTDSDRVKDPLVLLNRLAEQCVIAVTLEKVGGA 594 KK DE +K + GL + PL L+RL +Q ++A+ LEK G Sbjct: 538 RLVWKKRSSGIDDETVKSFETCILGLMRKGKG---SPLERLDRLVQQMMVAIQLEKAGDF 594 Query: 595 YGTNDTNSTS 604 D+NS++ Sbjct: 595 TDATDSNSST 604 >C1TQB6.1 C1TQB6_9BACT CRISPR-associated protein, Cmr2 family Length = 577 Score = 244 bits (622), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 182/539 (33%), Positives = 270/539 (50%), Gaps = 65/539 (12%) Query: 3 ISIGPVQEFIAQARKTRDLWFGSHLLSEISKEAARRLAEAHNARMIFPYIHKEKIAYGLD 62 ISIGPVQ FIA AR+TRDLWFGS LLSEI E AR + E + +IFP ++ G D Sbjct: 8 ISIGPVQPFIAAARRTRDLWFGSRLLSEICMETARSVRE-NGGVLIFPQKDSLNVSDGRD 66 Query: 63 DLKVANKVLAIVPTNDPKAVALDVRRAVVTKWLGYANKAKEELGSGIIGPMWERQVKDFI 122 VAN +LA +P DPK V R ++ GY ++ E G + W+ Q++ + Sbjct: 67 P-NVANVILAELPNGDPKEVVRQAREKALSVLKGYGDQVLGEYGELVNRDFWDDQIEQAL 125 Query: 123 EFYAVWSELESEEKYAEVLQQTEQLMAARKTLRDF-RPNEPADLFGDDKSGLDSGRESVL 181 EF++VW + + Y + + +LM ARK RDF +P +P D +KS LD ESV Sbjct: 126 EFFSVWVPYDGKSNYKDARDKVGRLMGARKNCRDFYQPGDP-DKRQVEKSSLDGRNESVF 184 Query: 182 KPSQYMSYA---------GFGIKHKETLDAISLVKRLSRFTVD-KKEFKSVCDVAFQQFR 231 K Q + +K E LDA+ L+KR++ D KK F SV D A + Sbjct: 185 KDRQLVEKVLKSDISKRLTLRLKSSEQLDALGLIKRVASPIDDSKKTFLSVDDFAASSWI 244 Query: 232 DELGASRNQWMKTAVDEYYRDLKHKYGTALKLTGSDLQSYKSEMFYTSRXXXXXXXXXXX 291 ++ + A +EY K+ AL LQ E + SR Sbjct: 245 EKA-------KRLAPEEY-----GKFEEALSKVKGLLQ---GEAAFPSR----ILELQDE 285 Query: 292 MKKPINRVKQLEITQNILTSLEELYGKILKEPTPYYALVVGDGDRMGDALRKMKTPAEHQ 351 MK+ ++ +K+ E K + EP PY+A++ DGD+MG A+ + P H+ Sbjct: 286 MKEDLSEIKE----------CREKLAKKIGEPNPYFAILKADGDKMGAAISSINDPEGHR 335 Query: 352 SFTEHLSGFASEVERIVFKDD---IKGQMIYSGGDDLMALLPVHRCLAAAQEIRQAFTNT 408 F+ L+GFA+E +I+ K D + + IY+GGDD++A LP+ C+ A+ + +AF + Sbjct: 336 VFSSDLAGFANEAHKILNKKDKERVIKRCIYAGGDDVLAFLPLDSCIECAKALAEAFEKS 395 Query: 409 MTLA---LPDEDQRPTLSVGIAIVHMFEPLEDSLLIARAAEALAK-VRRDELAIHFQKRS 464 + P D+ P+LSVGIA+ H EPLED L A AE LAK R+ LA+ + R Sbjct: 396 FEKSRGYFP-SDRAPSLSVGIAVGHFLEPLEDLLRFADEAETLAKEPDRNGLAVVVRSRG 454 Query: 465 GSEEMRVSLPFSE--------NPVNTIVKLQS-LYRNRYISTSFAYGLRQLHAEYLDLK 514 + + F E +PV+ ++ + L+ + IS+ F Y LR + Y + K Sbjct: 455 NA-----PIAFRERWDQYRGGSPVDVRLRCWAELFEDETISSRFPYELRSMVDFYRNWK 508 >C0DRI5.1 C0DRI5_NEICI Putative uncharacterized protein Length = 616 Score = 200 bits (508), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 183/640 (28%), Positives = 296/640 (46%), Gaps = 93/640 (14%) Query: 1 MLISIGPVQEFIAQARKTRDLWFGSHLLSEISKEAARRLAEAHNARMIFPYI---HKEKI 57 + +SIGPVQ FIA AR++RDLW GS LLSE +K AA+ L EA A M+FP++ K+++ Sbjct: 6 LTLSIGPVQGFIASARRSRDLWSGSWLLSETAKAAAKCLFEA-GALMVFPFVEEWQKDRL 64 Query: 58 AYG---LDDLKVANKVLAIVPTNDP---KAVALDVRRAVVTKWLGYANKAKEELGSGIIG 111 D+ V NK+ V D +A+ + A ++ +A+++L + Sbjct: 65 KPCDAMEDNFSVGNKIQVQVDAADDAEMRALVEQAKEAAQARFEAVVRQARQDLRAAARD 124 Query: 112 PMWERQVKDFIEFYAVWSELESEEKYAEVLQQTEQLMAARKTLRDFRPNEPADLFGD--- 168 +W+ QVKD++E A W+++ +E Y++ ++ + ARK RDF E + + + Sbjct: 125 EIWKVQVKDYLEIQAAWAQI-NEAGYSDASRRAASALVARKATRDFMQTEASGAWDERFM 183 Query: 169 -DKSGLDSGRESVLKPSQYMSYAG-----------FGIKHKETLDAISLVKRLSRFTVDK 216 KS LD RE+VL Y G G+ E LD + +VKRL + Sbjct: 184 LPKSSLDGARETVL----YEKADGDVDLTDAARRKLGLSRSEQLDCVGVVKRLGG---NS 236 Query: 217 KEFKSVCDVAFQQFRDELGASRNQWMKTAVDEYYRDLKHKYGTALKLTG-----SDLQSY 271 ++F + VA + EL + + A + A ++TG +D + Sbjct: 237 EQFTPITRVAADAWLQELPENELDRICKAYEPLV-----ALDLATRVTGNRGIYADF-PF 290 Query: 272 KSEMFYTSRXXXXXXXXXXXMKKPINRVKQLEITQNILTSLEELYGKILKEPTPYYALVV 331 +++ Y SR K + ++L N L + + YG EP Y+A+++ Sbjct: 291 DAQLLYRSRFDVALSDN----KYSADASEKLSDLGNALKPIWQKYG----EPCSYWAMLL 342 Query: 332 GDGDRMGDALRKMKTPAEHQSFTEHLSGFASEVERIVFKDDIKGQMIYSGGDDLMALLPV 391 DGDRMG+ L K KT HQ T LS FA V + + Q +Y+GGDD++ + + Sbjct: 343 ADGDRMGELLDKAKTIENHQEVTAALSKFAQGVPEKMRG--FRAQCVYAGGDDVLGFVSL 400 Query: 392 HRCLAAAQEIRQAFTNTMTLALPD--EDQRPTLSVGIAIVHMFEPLEDSLLIARAAEALA 449 +R + A +RQ F++ M D + PTLSVG+AI H+ PL + +A+ AE +A Sbjct: 401 NRAVDCADALRQTFSDGMKKVSADLGAENPPTLSVGLAICHINTPLGNVRRLAKRAEKVA 460 Query: 450 K-------VRRDELAIHFQKRSGSE-EMRVSLPFSENPVNTIVKLQSLYRNRYISTSFAY 501 K +R+ L I RSGS+ + RV + ++ + Q Y+ + IS+ AY Sbjct: 461 KGDGLPADRQRNGLGITLLVRSGSDSDWRVQWS-DKAGLDAFKQWQEYYQKKEISSRVAY 519 Query: 502 GLRQLHAEYLDLKAHGSLQNEGMGXXXXXXXXXXXXXKKPEFKS-------------KDE 548 R++ D L +E +K EF KD+ Sbjct: 520 DSREIFMR-TDFLPSADLSSE-----------LHQNIRKAEFDRMLDKARNMAGGELKDD 567 Query: 549 IIKEVLSVFKGLEQTDSDRVKDPLVLLNRLAEQCVIAVTL 588 + +++ + F L+Q DR D LN A + +IA L Sbjct: 568 VKRKLETRFAVLQQQLGDRQSDA---LNNFAVELIIARWL 604 >A5D0V5.1 A5D0V5_PELTS Predicted hydrolase Length = 602 Score = 195 bits (495), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 189/617 (30%), Positives = 288/617 (46%), Gaps = 86/617 (13%) Query: 5 IGPVQEFIAQARKTRDLWFGSHLLSEISKEAAR---RLAEAHNARMIFPYIHKEKIAYGL 61 IGPVQ+FIA AR++ DLW+GS LLSE++K AA R ++ +IFP + + Sbjct: 11 IGPVQDFIATARRSHDLWYGSWLLSELAKTAALEVVRYNGGDSSCLIFPASEGDSLEQLK 70 Query: 62 D-DLKVANKVLAIVPTNDPKAVALDVRRAVVTKWLGYANKAKEELGS----GIIGPMWER 116 D NKV+A+V N P + V+ ++ N+ +EL + GI G +ER Sbjct: 71 SFDFSAPNKVVAVVSCN-PVELGDAVKESI--------NRRLDELSNDVFRGING--FER 119 Query: 117 -----QVKDFIEFYAVWSELESEEKYAEVLQQTEQLMAARKTLRDFR-----PNEPADLF 166 QVKDFIEF+ W+ Y + E +++ARK RDF +EP Sbjct: 120 EVAVQQVKDFIEFF--WASYPLNGNYKQARDFAEAVLSARKATRDFAQVSWGSSEP---- 173 Query: 167 GDDKSGLDSGRESVLKPSQYMS------YAGFGIKHKETLDAISLVKRLSRFTVDKKEFK 220 K LD RESV+ + Y Y + ++ E L + L+KR + ++ F Sbjct: 174 ---KCSLDGCRESVIAETIYKQMNDDKLYKSYRVQRGEYLCGVCLLKRHGKRGREEYFFS 230 Query: 221 SVCDVAFQQFRDELGASRNQWMKTAVDEYYRDLKHKYGTALKLTGSDLQSYKSEMFYTSR 280 + A G R V+EY LK L + DL++ + Sbjct: 231 TSHVAALPLLERLTGQHR-----PLVEEYVGKLKE-----LGIPPGDLETVRPPHPVFGP 280 Query: 281 XXXXXXXXXXXMKKPINRVKQLEITQNILTS-LEELYGKILKEPTPYYALVVGDGDRMGD 339 + ++++I Q L L++ +G K+P PYYAL+ DGD MG Sbjct: 281 NDGHLLYEERFREFFFQEKEKIQIAQKALREFLKKAFGD--KKPLPYYALLCADGDHMGK 338 Query: 340 ALRKMKTPAEHQSFTEHLSGFASEVERIVFKDDIKGQMIYSGGDDLMALLPVHRCLAAAQ 399 + KTP +H+ + S FA E RIV +++ G +IYSGGDD++AL+P+H+ L A Sbjct: 339 VIDAQKTPEKHRELSRSQSSFALEARRIVKENN--GSLIYSGGDDVLALVPLHKVLDCAC 396 Query: 400 EIRQAFTNTMTLALPDEDQ----RPTLSVGIAIVHMFEPLEDSLLIARAAEALAKV--RR 453 + + F + ED PTLSVGIA+ H EPL D L +AR+AE AK + Sbjct: 397 RLSKTFRERLA-GFKGEDGGEEISPTLSVGIAVAHHLEPLSDVLELARSAEKAAKSVPGK 455 Query: 454 DELAIHFQKRSGSEEMRVSLPFSENPVNTIVK-LQSLYRNRYISTSFAYGL----RQLHA 508 + LA+ KRSGSE M + + ++ +K L +L+RN + AY L R+L Sbjct: 456 NALAVTLSKRSGSERM---VKGTWGDLDRQLKWLINLHRNEDVPDGAAYELYDLARRLET 512 Query: 509 EYLDLKAHGSLQNEGMGXXXXXXXXXXXXXKKPEFKSKDEIIKEVLSVFKGLEQTDSDRV 568 +LK G+LQ +K + + + +EVL+ + L ++D Sbjct: 513 RNEELK--GTLQEAACA------EAKRILRRKKARRGTERMAEEVLAGLESLLESD---- 560 Query: 569 KDPLVLLNRLAEQCVIA 585 K L +LA+ +IA Sbjct: 561 KFSFEKLKQLADGLIIA 577 >B1I5L0.1 B1I5L0_DESAP CRISPR-associated protein, Crm2 family Length = 607 Score = 190 bits (483), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 160/487 (32%), Positives = 235/487 (48%), Gaps = 44/487 (9%) Query: 1 MLISIGPVQEFIAQARKTRDLWFGSHLLSEISKEAARRLAEAHN---ARMIFPYIHKEKI 57 ++ IGPVQ+FIA AR++RDLWFGS LLSE++K AA + + + + ++FP K + Sbjct: 7 VVFHIGPVQDFIATARRSRDLWFGSWLLSELAKAAALEIVQQNGNDISCLVFPAPDKLEQ 66 Query: 58 AYGLDDLKVANKVLAIVPTNDPKAVALDVRRAVVTKWLGYANKAKEELGSGIIGPMWERQ 117 D NKV+A V DP + VR A++ + + A +++ G + +RQ Sbjct: 67 LKSF-DFNAPNKVVARVRC-DPAELRESVREAILRRLCEICDDAYKKIKGGFDREIAKRQ 124 Query: 118 VKDFIEFYAVWSELESEEKYAEVLQQTEQLMAARKTLRDFRP---NEPADLFGDDKSGLD 174 V+DF EF+ W+ Y + E L+ ARK R+F P PA K LD Sbjct: 125 VEDFPEFF--WASYPFNGNYKQARDFAEALLNARKVTRNFAPITWGSPA-----PKCSLD 177 Query: 175 SGRESVLKPSQYMS------YAGFGIKHKETLDAISLVKRLSRFTVDKKEFKSVCDVAFQ 228 RESV+ S Y Y + ++ E L + L+KR R ++ F S VA Sbjct: 178 GCRESVITGSVYSRMNESQLYENYRVQRGEYLCGVCLLKRHGR-RGSEEYFFSTSHVAAL 236 Query: 229 QFRDELGASRNQWMKTAVDEYYRDLKHKYGTALKLTGSDLQS--YKSEMFYTSRXXXXXX 286 + L NQ + VD Y LK L +T LQ+ +F Sbjct: 237 PLLERLT---NQ-HQPLVDAYIGKLKE-----LGITADALQTVWLHHPVFGPHDGQLLFE 287 Query: 287 XXXXXMKKPINRVKQLEITQNILTSLEELYGKILKEPTPYYALVVGDGDRMGDALRKMKT 346 K K L+ + + T L++ + K+P PYY L++ DGD +G + +T Sbjct: 288 ERLREFFKEEEEGKLLQAKEALRTFLKQAFDS--KKPLPYYTLLLADGDHVGKVIDTQET 345 Query: 347 PAEHQSFTEHLSGFASEVERIVFKDDIKGQMIYSGGDDLMALLPVHRCLAAAQEIRQAFT 406 P +HQ + S FA EV IV +G ++YSGGDD++AL+P+H LA A+ + + F Sbjct: 346 PEKHQELSRSQSRFALEVRDIV--QHHQGSLLYSGGDDVLALVPLHTVLACARSLAETFR 403 Query: 407 NTMTLALPDEDQRP----TLSVGIAIVHMFEPLEDSLLIARAAEALAKV--RRDELAIHF 460 ED TLSVGIA+VH +PL D+L +AR AE AK ++ LA+ Sbjct: 404 QRFA-GFKAEDGGKEISLTLSVGIAVVHHLDPLSDALELARRAEKAAKSVGGKNALAVTL 462 Query: 461 QKRSGSE 467 KR G E Sbjct: 463 SKRGGVE 469 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,297,302,693 Number of extensions: 129373201 Number of successful extensions: 315048 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 314940 Number of HSP's successfully gapped: 73 Length of query: 604 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 462 Effective length of database: 1,722,912,073 Effective search space: 795985377726 Effective search space used: 795985377726 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)