BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0057.p (434 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WL19.1 Q5WL19_BACSK Lactose ABC transporter substrate-binding ... 236 3e-60 Q5WL28.1 Q5WL28_BACSK Lactose ABC transporter substrate-binding ... 236 4e-60 Q9KDU4.1 Q9KDU4_BACHD BH1117 protein 221 1e-55 B3KGV1.1 B3KGV1_9BACI Extracellular solute-binding protein family 1 218 8e-55 B5YC84.1 B5YC84_DICT6 Lactose ABC transporter substrate-binding ... 212 5e-53 >Q5WL19.1 Q5WL19_BACSK Lactose ABC transporter substrate-binding protein Length = 470 Score = 236 bits (603), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 139/426 (32%), Positives = 234/426 (54%), Gaps = 24/426 (5%) Query: 16 VMLVTLVGC-TGKGNEEVQKSDE-QITLNFMWWGKPSRKEITLKVIELFEKENPNIKINT 73 + L+ + GC G G E+ + + L WWG R +TL+VI+LFE++ P+I + Sbjct: 53 LALLAMAGCQVGTGGEDNRSTASGDNVLRVAWWGGQERHTMTLEVIDLFEEKYPDISVEP 112 Query: 74 EDYPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNILDLSDVD 133 E Y S ++L A PD++Q+DY Y++ +L++ L P ++ +DLS+VD Sbjct: 113 E-YTSWDNYWERLTTQAAGSNLPDVVQLDYTKMNEYISRNLLKDLAPLIEDGSIDLSNVD 171 Query: 134 KSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQQFKDKV 193 Y + YGI +GSNALGM Y+ +F ++GI+ L + YT +D++ +M + K + Sbjct: 172 DVYQDVNTIDDAIYGISLGSNALGMLYNAELFAEHGIE-LDEGYTYEDMKASMLKLKSAL 230 Query: 194 QTPDFYPL---NAMFDLSY-WLRMKGESFYNKEKTGLGYTDATMIEYLTLIKTWLDLGLL 249 FY N FDL + + R GES +N+ GLGY+D T++ Y I+ G++ Sbjct: 231 -GDGFYGYDFNNTEFDLFFAYARQNGESVFNETGDGLGYSDETLVSYFQFIQ-----GMV 284 Query: 250 NPGTASTTE---------ENPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQGAA 300 A E ++ +A G T+ L SNQ++ L + LP +G Sbjct: 285 EEEAAPPHELTMEYIQGGDSTIADGTTSMLLIASNQIIGQQALTEAELDLKPLPEMEGGT 344 Query: 301 AEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLSNE 360 EGN+I+PSM +S ++ +E A F+ F+TND EAN IL+ ERGVP++A++ L + Sbjct: 345 -EGNWIRPSMSFAISEHTNQEEHAALFIDFITNDPEANEILQAERGVPISAEIRSHLEGK 403 Query: 361 ATEQGKEQYSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLYAGSITPEAAASSFRA 420 + + ++ +++++Y+A++S P DP P ++ ++ + ++++L G TPE A FR Sbjct: 404 VSPEVEKTFAYLEYVAENSAPADPLPPPGESEVRAAFLRVVESLKYGQTTPEDGAEQFRQ 463 Query: 421 QAEDVL 426 QAE +L Sbjct: 464 QAESIL 469 >Q5WL28.1 Q5WL28_BACSK Lactose ABC transporter substrate-binding protein Length = 437 Score = 236 bits (601), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 133/428 (31%), Positives = 219/428 (51%), Gaps = 16/428 (3%) Query: 13 LAFVMLVTLVGCT--------GKGNEEVQKSDEQITLNFMWWGKPSRKEITLKVIELFEK 64 + V +V L C+ G+G E+ Q L WWG +R + T++ I+LFE Sbjct: 10 MCLVSVVALTACSSSETEVAEGEGGEKADLKSGQTELRVAWWGSQARHDRTIEAIKLFEA 69 Query: 65 ENPNIKINTEDYPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKS 124 ENP+I I TE + ++++ A + PD++QMD + YV+ DL+ L PYV+ Sbjct: 70 ENPDIAITTE-FTGWDGYWERMSTQAAGRNLPDVIQMDLQYINEYVSRDLLVDLTPYVED 128 Query: 125 NILDLSDVDKSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQ 184 LD SDVD YL G YGI +GSNA+ +AYD +F + G+ L+ YT D + Sbjct: 129 GTLDFSDVDDIYLEGGIVNEGLYGINLGSNAMSIAYDAEIFAEAGVPELEPGYTWDDYIE 188 Query: 185 TMQQFKDKVQTPDFYPLNAMFDL-SYWLRMKGESFYNKEKTGLGYTD-ATMIEYLTLIKT 242 ++ + + Y + ++LR YN++ TGLGY D +I++L + K Sbjct: 189 AVKTIHENLDGMYAYGFGDSLNFFKHYLRQHDLWLYNEDNTGLGYDDDKYLIDFLNMYKD 248 Query: 243 WLDLGLLNP----GTASTTEENPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQG 298 LD G+ P ++ + G+ + SNQ+V++ + AGR L++ LP +G Sbjct: 249 LLDSGVAAPPDVKAEVQGIQDELIVHGRASTFSPHSNQIVALNEAAGRKLQMTTLPAMEG 308 Query: 299 AAAEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLS 358 + E +FIKPS+ +S + E A +F++F N EAN IL ERGVP++ KV + L Sbjct: 309 GS-EASFIKPSLLFSATSQGDKSEEAARFINFFVNSIEANEILNAERGVPISEKVRDHLY 367 Query: 359 NEATEQGKEQYSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLYAGSITPEAAASSF 418 + +E + Q+ +++ + + + PI PP P I + +++ ++ E A F Sbjct: 368 DHVSENTQLQFDYVQLVEQRAAPIHPPDPAGTGQILDLYARMIEEFEYELLSAEEFAERF 427 Query: 419 RAQAEDVL 426 R + E +L Sbjct: 428 RGEVEIIL 435 >Q9KDU4.1 Q9KDU4_BACHD BH1117 protein Length = 434 Score = 221 bits (562), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 141/428 (32%), Positives = 233/428 (54%), Gaps = 18/428 (4%) Query: 12 ILAFVMLVTLVGCT-GKGNE---EVQKSDEQITLNFMWWGKPSRKEITLKVIELFEKENP 67 +L L+ L GC+ G NE E + +E + L WWG R ++T++ IELFE++ P Sbjct: 11 LLGVAFLLVLAGCSSGTSNEVDGEQEAGNEDVVLRVAWWGGQERHDMTIEAIELFEEKYP 70 Query: 68 NIKINTEDYPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNIL 127 +I+++ E + S ++L A PD++QMD + Y + L+ L + + Sbjct: 71 HIQVDPE-FTSWDNYWERLTTQAAGNNLPDVIQMDNSKLNEYSSRGLVIDLQSLIADGTI 129 Query: 128 DLSDVDKSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQ 187 +L DVD Y +G + + GSNAL Y+ M E+ GIQ L+ YT +D + MQ Sbjct: 130 NLDDVDDVYQDMNVQDGSVWAVSAGSNALAAIYNEEMLEEQGIQ-LEPGYTYEDFYEAMQ 188 Query: 188 QFKDKVQTPDFYPL---NAMFDLSY-WLRMKGESFYNKEKTGLGYTDATMIEYLTLIKTW 243 K+ + DFY NA +++ + + R GESFYN++ TGLG+ + T+I++LT +K Sbjct: 189 TLKENIDG-DFYGYDFGNAEYEMFFVYARQNGESFYNEDGTGLGFENQTLIDFLTFVKQM 247 Query: 244 LDLGLLNPGTASTTE-----ENPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQG 298 +D G+ P A T E E+ L + SN ++ + L +M LP+ G Sbjct: 248 VDDGVAPP-HALTLEYIQGGESLLGDQMAGAAMAASNGIIGLQQSTEHQLGLMLLPSLDG 306 Query: 299 AAAEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLS 358 GN+I+PSM +SS+SE +EAA F+ FLTND +AN ILK ERGVP+++KV E L+ Sbjct: 307 GV-HGNWIRPSMSYSISSHSEEQEAAALFIDFLTNDLQANEILKAERGVPISSKVREHLA 365 Query: 359 NEATEQGKEQYSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLYAGSITPEAAASSF 418 + + F++ +A +++P DP P ++ ++ + +++++ ++ E A F Sbjct: 366 PMVDGPIAKTFEFLELVADYTSPADPLPPPGESEVRGAFLRMIESVKYERLSIEEATEQF 425 Query: 419 RAQAEDVL 426 R +AE +L Sbjct: 426 RQEAEQIL 433 >B3KGV1.1 B3KGV1_9BACI Extracellular solute-binding protein family 1 Length = 446 Score = 218 bits (556), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 137/441 (31%), Positives = 226/441 (51%), Gaps = 27/441 (6%) Query: 9 VAKILAFVMLVTLVGCTGKGNEE---------------VQKSDEQITLNFMWWGKPSRKE 53 ++ +L F ++ +L+GC+G G E S + + L WWG SR + Sbjct: 5 LSAVLMFSLIGSLIGCSGGGAAEPPANGGTGTETPPATASDSKDPVKLRIAWWGGQSRHD 64 Query: 54 ITLKVIELFEKENPNIKINTEDYPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHD 113 TLKVIE++E +NP++ I E Y + +KLA PD++QMD ++ Y + Sbjct: 65 YTLKVIEMYESQNPHVTIEPE-YAPFDDYWKKLAPQAVAGGLPDIIQMDISYLNQYAGRN 123 Query: 114 LIEPLDPYVKSNILDLSDVDKSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPL 173 + L P+ +S LD++DV ++ L G+ GK + +G NAL +D ++ GI Sbjct: 124 QLADLKPFTESGELDVADVSENALRGGEVNGKLVAMNLGVNALQTTFDVETMKKNGIDIP 183 Query: 174 QDPYTIQDLQQTMQQFKDK-VQTPDFYPLNAMFDLSYWLRMKGESFYNKEKTGLGYTD-A 231 +T D+ + Q K+K +Q F + +F Y+LR G+ Y+ + +GY+D Sbjct: 184 SKEWTWDDMDKMGAQAKEKGLQIGGFAYRHDVF-FPYYLRTIGQKMYSADGKTIGYSDDQ 242 Query: 232 TMIEYLTLIKTWLDLGLL-----NPGTASTTEENPLAAGKTAFLQSVSNQMVSIGD-EAG 285 I+Y T + W D G + EE+ + G + SNQ +++ + Sbjct: 243 PFIDYFTRYQKWYDNGYILSLDKEAQKKGVAEEDEIVLGNSISGTGWSNQFLAVANLVTD 302 Query: 286 RTLKIMNLPTAQGAAAEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGER 345 R+L++ +P +G F+KPSM+ V+ S+HKE A KF+SF ND EAN ++KGER Sbjct: 303 RSLELNPMPGP--GTKDGLFLKPSMYFSVTETSKHKEEAAKFISFFINDIEANKLIKGER 360 Query: 346 GVPVAAKVAEQLSNEATEQGKEQYSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLY 405 GVPV+AKV E L +E + + ++ + +STP DPP P+ I+ L+ + + + Sbjct: 361 GVPVSAKVKEALKPLLSENEAKIFDYVTWAEANSTPGDPPNPIGAVEIEKLLRDLSERIL 420 Query: 406 AGSITPEAAASSFRAQAEDVL 426 I+ E AA+ FR +A +L Sbjct: 421 FKKISIEDAAAEFRKEANAIL 441 >B5YC84.1 B5YC84_DICT6 Lactose ABC transporter substrate-binding protein Length = 422 Score = 212 bits (540), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 127/405 (31%), Positives = 210/405 (51%), Gaps = 11/405 (2%) Query: 32 VQKSDEQITLNFMWWGKPSRKEITLKVIELFEKENPNIKINTEDYPSTGEVAQKLAMDTA 91 V S + I L WWG +R TL+VI+LFEK P IKI T +Y S + KL A Sbjct: 19 VSYSQQPINLRVAWWGSQTRHNQTLEVIKLFEKRYPYIKI-TPEYTSWSDYWTKLNTQAA 77 Query: 92 EQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNILDLSDVDKSYLVSGQYEGKQYGIPM 151 Q PD++Q DY + +V L+ PLD +VK+N+++L DV +Y+ G GK YGI + Sbjct: 78 AQNLPDVIQHDYQYITEWVNRGLLLPLDDFVKNNVINLKDVAPNYIKPGIVNGKLYGINL 137 Query: 152 GSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQQFKDKVQTPDFYPLNAMFD-LSYW 210 G NALG+ YDP +F+Q G+ ++ +T + + T+ + K+K+ + W Sbjct: 138 GVNALGLVYDPEIFKQAGVPTPKNDWTWDEFKDTVIRIKNKLGIYGVEGFTGQIECFKIW 197 Query: 211 LRMKGESFYNKEKTGLGYTDATMIEYLTLIKTWLDLGLLNPGTAST-------TEENPLA 263 L GE Y + +G++ T +++ ++ D G P A E+ + Sbjct: 198 LIENGEWLYASDGKSIGFSKDTFVKWWKMMLDIEDAGAA-PNIAVDVSRSGLGVEDQFIV 256 Query: 264 AGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQGAAAEGNFIKPSMFIMVSSYSEHKEA 323 K+A + SNQ+++I AGR LK++ P + G ++K SMF +++ +++ + Sbjct: 257 TKKSAMSLAWSNQLIAISRAAGRPLKMVLFPRLDKKSKSGTYLKASMFFSINARTKYPKE 316 Query: 324 AVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLSNEATEQGKEQYSFMKYIAKHSTPID 383 + F+ F TN EA+ I+K ERGVP++++V L KE ++F+K K +P Sbjct: 317 SAMFIDFFTNSLEAHKIMKAERGVPISSRVQTFLKPYLEAPQKEAFAFVKLAEKEGSPTP 376 Query: 384 PP-APLSDAVIQNSLKLILKNLYAGSITPEAAASSFRAQAEDVLD 427 PP P V N + I + + I+ E A ++ + +AE +L+ Sbjct: 377 PPDIPKHAEVSNNVIAPIWEQIRYKKISIEDAYNNLKKEAERILN 421 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.130 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,536,372,461 Number of extensions: 105374833 Number of successful extensions: 270463 Number of sequences better than 1.0e-05: 698 Number of HSP's gapped: 270316 Number of HSP's successfully gapped: 698 Length of query: 434 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 295 Effective length of database: 1,751,175,118 Effective search space: 516596659810 Effective search space used: 516596659810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)