BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0055.p (555 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P49939.2 GERKA_BACSU Spore germination protein KA 494 e-138 A3F901.1 A3F901_BACME GerUA 488 e-136 Q9KBJ3.1 Q9KBJ3_BACHD Spore germination protein KA 483 e-134 O07504.1 O07504_BACME Germination protein 480 e-133 A7Z1B0.1 A7Z1B0_BACA2 GerKA 478 e-133 >P49939.2 GERKA_BACSU Spore germination protein KA Length = 544 Score = 494 bits (1273), Expect = e-138, Method: Compositional matrix adjust. Identities = 245/510 (48%), Positives = 347/510 (68%), Gaps = 16/510 (3%) Query: 42 QAELGNSPDLIIRQIQLGGPLRIQAAAVHLSGLADTTAVNQFVIGSLLGNTESLFADIXX 101 + E+G+S D+IIR+I++G + A +++SGL D +++ +I L+ + ES+ Sbjct: 48 KKEVGSSSDVIIREIKIGEQDHVHLAVIYISGLVDNNTIHESLIDPLVQD-ESI------ 100 Query: 102 XXXXXXRLPAMILSRALEIGEAEIKEEWKEMMLAVLSGDTAILLDGYAASILCATRGGEW 161 IL + L +G + ++ W ++ ++ G+ I DG+ +++C+T+GGE Sbjct: 101 ---QNTHAIQQILEKTLPLGGVKAEKSWDKLFSELMLGNALIFADGHDEALICSTQGGEQ 157 Query: 162 RSVSEPTSQLVVRGPKDGFVESVATNISLIRRRIKSSKLRLEYMKIGSETQTLVAMMYVK 221 RS+ EP++Q+ RGP+ GF ES+ TNIS+IRR IK+ L +E MK GS T T +A+MY++ Sbjct: 158 RSIQEPSTQVSFRGPRQGFTESLQTNISMIRRYIKNPNLWVEKMKKGSVTNTDIALMYIQ 217 Query: 222 DIAGEDLISEVRERLGKIAMNEVLESGYIEELIQDKTFTPFPTIYNTERPDVAAGNLLEG 281 I E ++ EV++RL KI ++ +LESGYIE+LI+D+TFT FPT+Y+TERPDV AGNLLEG Sbjct: 218 GICDEKVLKEVKQRLEKIDIDSILESGYIEQLIEDETFTTFPTMYHTERPDVVAGNLLEG 277 Query: 282 RVVIIVDGTPFVLILPAVFTQFFQSAEDYSQRFDIAILMRVVRYMSFIVLILGPSVYIAL 341 R IIVDGTPFVLI PA+F QFFQS EDY RFDIA +R++R + F + ++ P+VY+A Sbjct: 278 RFAIIVDGTPFVLIAPALFVQFFQSVEDYYSRFDIATSIRILRVLVFFISLVAPAVYVAA 337 Query: 342 TTYHYEMIPTTLLINLLAQRENVPFPALVEALLMEGAFEILREAGVRMPRVIGQTVSVVG 401 TT+H EMIPT LL+ + AQRE VPFPA+VEAL ME AFEILREAGVR+PRV+G VS+VG Sbjct: 338 TTFHQEMIPTQLLVVIAAQREIVPFPAVVEALTMEVAFEILREAGVRLPRVVGSAVSIVG 397 Query: 402 ALILGSAVVEAGIITPIMVIVVALTGIASFAIPAYNMSIAGRLIRXXXXXXXXXXXXYGV 461 AL++G A V+AGI++P MVI+VALT IASFA PA+ M+I+ RLIR YG+ Sbjct: 398 ALVIGQAAVQAGIVSPAMVIIVALTAIASFATPAFAMAISARLIRFIFIIASAVMGFYGL 457 Query: 462 ILGLIVMVAHMNSLRSFGVPYLSPFVPLSVKGQKDTILRLPLWLMTPRKSPQQMRAEQPE 521 ILG+I+M H+ SLRSFGVPY+SP P S +G KD + R+P W K P+ + E Sbjct: 458 ILGIIMMFVHLCSLRSFGVPYMSPLAPFSSQGVKDALFRVPWW--ADEKRPESVSKED-- 513 Query: 522 FMRLTTGNEELLAPLIGKADNPSEATEGDR 551 ++ G ++ P + + EGD+ Sbjct: 514 --KVRQGKDQRPEPAASRGMVNKDLEEGDQ 541 >A3F901.1 A3F901_BACME GerUA Length = 540 Score = 488 bits (1255), Expect = e-136, Method: Compositional matrix adjust. Identities = 234/502 (46%), Positives = 332/502 (66%), Gaps = 7/502 (1%) Query: 8 KRSDKAAHTDQKGSSQTGRLTGXXXXXXXXXAIIQAELGNSPDLIIRQIQLGGPLRIQAA 67 ++S K D K + II+ + GNS D++IR +++G I+ A Sbjct: 8 RKSKKNRQKDAKNQATDQSAEHIQDSLSANLNIIKQKTGNSSDVVIRHLKMGSNSNIRVA 67 Query: 68 AVHLSGLADTTAVNQFVIGSLLGNTESLFADIXXXXXXXXRLPAMILSRALEIGEAEIKE 127 V+L G+ D ++ +F++ S++ + E +I + +G Sbjct: 68 IVYLEGIVDNQSIQEFLLESMMKDNEK-------EKVNEHNALELISEDMMAVGNVTFIH 120 Query: 128 EWKEMMLAVLSGDTAILLDGYAASILCATRGGEWRSVSEPTSQLVVRGPKDGFVESVATN 187 W ++ A++ GDT L+DG ++ T+GGE RS++E T+Q+VVRGPK F ES+ TN Sbjct: 121 NWDDLFSALMVGDTLFLVDGIDQALGAGTQGGEKRSIAESTTQMVVRGPKGAFTESIGTN 180 Query: 188 ISLIRRRIKSSKLRLEYMKIGSETQTLVAMMYVKDIAGEDLISEVRERLGKIAMNEVLES 247 +++RR IK+ L E KIG T+T V +MY+ DIA + +I+E+R+RL KI ++ +LES Sbjct: 181 TAMVRRIIKTPDLWTESFKIGRVTKTDVTLMYIHDIANDKVINEIRQRLNKIDVDSILES 240 Query: 248 GYIEELIQDKTFTPFPTIYNTERPDVAAGNLLEGRVVIIVDGTPFVLILPAVFTQFFQSA 307 GYIE+LI+D+T T FPTIYNTERPDV AGNLLEGR+ I VDGTPFVLI PAVF QFFQSA Sbjct: 241 GYIEQLIEDETMTSFPTIYNTERPDVVAGNLLEGRIAIFVDGTPFVLIAPAVFMQFFQSA 300 Query: 308 EDYSQRFDIAILMRVVRYMSFIVLILGPSVYIALTTYHYEMIPTTLLINLLAQRENVPFP 367 EDY RFDIA +R++R F++ ++ P+ Y+A+TT+H EM+PTTL++ + AQRE VPFP Sbjct: 301 EDYYARFDIATSIRLLRIFMFLISLIAPATYVAVTTFHQEMVPTTLIVAIAAQREAVPFP 360 Query: 368 ALVEALLMEGAFEILREAGVRMPRVIGQTVSVVGALILGSAVVEAGIITPIMVIVVALTG 427 A VEALLME FEILREAG+R+P+ IG VS+VG+L++G A V+A I++P MVI+V++T Sbjct: 361 AFVEALLMEVTFEILREAGIRLPKAIGSAVSIVGSLVIGQAAVQASIVSPAMVIIVSITA 420 Query: 428 IASFAIPAYNMSIAGRLIRXXXXXXXXXXXXYGVILGLIVMVAHMNSLRSFGVPYLSPFV 487 IASFA P+++M+I+ RLIR YG+IL L++MV H+ SLRSFGVPY++PF Sbjct: 421 IASFATPSFDMAISARLIRFLFMLGAATFGFYGIILVLLMMVVHLCSLRSFGVPYMAPFA 480 Query: 488 PLSVKGQKDTILRLPLWLMTPR 509 P DT++RLP W + R Sbjct: 481 PFIPVNNGDTVMRLPWWTLRQR 502 >Q9KBJ3.1 Q9KBJ3_BACHD Spore germination protein KA Length = 515 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 229/466 (49%), Positives = 333/466 (71%), Gaps = 4/466 (0%) Query: 41 IQAELGNSPDLIIRQIQLGGPLRIQAAAVHLSGLADTTAVNQFVIGSLLGNTESLFADIX 100 I+ LGNS DL+IR++ + I AV ++GLAD V++ ++ + L ++ A Sbjct: 43 IKELLGNSSDLVIRELMIIHEPEISGVAVFINGLADEQLVSECIVSATLHPSKE--AHYF 100 Query: 101 XXXXXXXRLPAMILSRALEIGEAEIKEEWKEMMLAVLSGDTAILLDGYAASILCATRGGE 160 RL ++I + L + + + W ++ ++LSG++ +L+DG+ ++ C+T G + Sbjct: 101 KEDTKDKRL-SIIEKQILSVARVQAYDLWNPIIHSLLSGNSIVLIDGFDKALSCSTPGSK 159 Query: 161 WRSVSEPTSQLVVRGPKDGFVESVATNISLIRRRIKSSKLRLEYMKIGSETQTLVAMMYV 220 WRS+ EP+++ VRGPKD F ES+ TN+SLIRRR+KS++LR+E ++G T T VA++YV Sbjct: 160 WRSIEEPSTETTVRGPKDAFNESLQTNLSLIRRRVKSNRLRVENTELGETTHTDVAIVYV 219 Query: 221 KDIAGEDLISEVRERLGKIAMNEVLESGYIEELIQDKTFTPFPTIYNTERPDVAAGNLLE 280 D+ + L+ E+++RL ++ ++V+ IEE +QD+ TPFPT YNTERPD+ A L+E Sbjct: 220 DDLVDKKLLQEIKQRLNRVITHKVISEANIEEAMQDEMLTPFPTSYNTERPDMVAYALVE 279 Query: 281 GRVVIIVDGTPFVLILPAVFTQFFQSAEDYSQRFDIAILMRVVRYMSFIVLILGPSVYIA 340 G+V +I+DGTPF LILP F QFF++AEDY QR+D++ +R++RY +F V +LGPS+Y+A Sbjct: 280 GKVALIIDGTPFALILPTDFAQFFKAAEDYYQRYDLSTFIRLLRYFAFTVSLLGPSLYVA 339 Query: 341 LTTYHYEMIPTTLLINLLAQRENVPFPALVEALLMEGAFEILREAGVRMPRVIGQTVSVV 400 L T+H ++IPTTLLI L +QRE +PFPALVEAL+ME FE+LREAG+RMPR IGQ VS+V Sbjct: 340 LITFHQDLIPTTLLIRLASQREGIPFPALVEALIMEFTFELLREAGLRMPRAIGQAVSIV 399 Query: 401 GALILGSAVVEAGIITPIMVIVVALTGIASFAIPAYNMSIAGRLIRXXXXXXXXXXXXYG 460 GAL++G A V+AGI++ MVIVVA TGIASF PAYN+++A RL+R YG Sbjct: 400 GALVIGQAAVQAGIVSAAMVIVVAATGIASFTTPAYNLAVAARLLRFFIMLLAATFGLYG 459 Query: 461 VILGLIVMVAHMNSLRSFGVPYLSPFVPLSVKGQKDTILRLPL-WL 505 +ILGLI++VAH++SLRS G+PY +PF P S+K QKD + RLPL WL Sbjct: 460 IILGLIMLVAHLSSLRSVGMPYFAPFAPFSMKAQKDGLFRLPLKWL 505 >O07504.1 O07504_BACME Germination protein Length = 540 Score = 480 bits (1235), Expect = e-133, Method: Compositional matrix adjust. Identities = 234/526 (44%), Positives = 348/526 (66%), Gaps = 19/526 (3%) Query: 4 KGQLKRSDKAAHTDQKGSSQTGRLTGXXXXXXXXXAIIQAELGNSPDLIIRQIQLGGPLR 63 K Q ++ DQ + QT ++ +I+ + GNS D++IR ++G Sbjct: 6 KNQKSKNKLQPQADQTNNEQT--VSSLKNLLSENLTLIKEKTGNSSDIVIRHFKIGVNNS 63 Query: 64 IQAAAVHLSGLADTTAVNQFVIGSLLGNTESLFADIXXXXXXXXRLPAMILSRALEIGEA 123 + AA V++ G+ D A+ +++ SL+ + + D+ +I + +G Sbjct: 64 LAAAIVYIEGIVDNQAIQDYLLQSLMKDNQK--NDLNDQNAL-----ELISEDIVTMGNV 116 Query: 124 EIKEEWKEMMLAVLSGDTAILLDGYAASILCATRGGEWRSVSEPTSQLVVRGPKDGFVES 183 + W +++ +++SGD+ +++DG + +T+GGE R+++E T+Q+VVRGPK F ES Sbjct: 117 SFADNWNDLLSSLMSGDSLLIVDGINRVLSVSTQGGEKRAIAESTTQMVVRGPKGAFTES 176 Query: 184 VATNISLIRRRIKSSKLRLEYMKIGSETQTLVAMMYVKDIAGEDLISEVRERLGKIAMNE 243 + TN++++RR IK+ L LE MKIG T+T V +MY+ IA + ++ E+R+RL I ++ Sbjct: 177 IGTNLAMVRRIIKTPDLWLESMKIGRVTKTDVTLMYIHGIANDKVVKEIRKRLKNIDIDS 236 Query: 244 VLESGYIEELIQDKTFTPFPTIYNTERPDVAAGNLLEGRVVIIVDGTPFVLILPAVFTQF 303 +LESGY+E+LI+D+T TPFPTIYNTERPDV AGNLLEGR+ I VDGTPF LI PA+F QF Sbjct: 237 ILESGYVEQLIEDQTVTPFPTIYNTERPDVVAGNLLEGRIAIFVDGTPFGLIAPALFIQF 296 Query: 304 FQSAEDYSQRFDIAILMRVVRYMSFIVLILGPSVYIALTTYHYEMIPTTLLINLLAQREN 363 FQSAEDY RFDIA +R++R F++ ++ P+ Y+A+TT+H EM+PTTL++ L AQRE+ Sbjct: 297 FQSAEDYYARFDIATSIRLLRVFMFMISLIAPATYVAVTTFHQEMVPTTLIVALAAQRES 356 Query: 364 VPFPALVEALLMEGAFEILREAGVRMPRVIGQTVSVVGALILGSAVVEAGIITPIMVIVV 423 VPFPA VEA ME FEILREAG+R+P+ IG VS+VGAL++G A V+AGI++P MVI+V Sbjct: 357 VPFPAFVEAFFMEVTFEILREAGIRLPKAIGSAVSIVGALVIGQAAVQAGIVSPAMVIIV 416 Query: 424 ALTGIASFAIPAYNMSIAGRLIRXXXXXXXXXXXXYGVILGLIVMVAHMNSLRSFGVPYL 483 ++T IASFA P+++M+I+ RLIR YG+IL L++ V H+ SLRSFGVPY+ Sbjct: 417 SITAIASFATPSFDMAISARLIRFLFILGAATFGFYGIILILLMGVGHLCSLRSFGVPYM 476 Query: 484 SPFVPLSVKGQKDTILRLPLWLMTPRKSPQ--------QMRAEQPE 521 +P P +DT+LRLP W M R+ P+ Q + ++PE Sbjct: 477 TPLAPFIPVSNEDTLLRLPWWTM--RRRPKLISDNKVRQSKHQKPE 520 >A7Z1B0.1 A7Z1B0_BACA2 GerKA Length = 546 Score = 478 bits (1229), Expect = e-133, Method: Compositional matrix adjust. Identities = 237/460 (51%), Positives = 321/460 (69%), Gaps = 11/460 (2%) Query: 45 LGNSPDLIIRQIQLGGPLRIQAAAVHLSGLADTTAVNQFVIGSLLGNTESLFADIXXXXX 104 GNS D++IR++++G + AA V+++GL D V+Q ++ L I Sbjct: 53 FGNSADVVIRRLKIGKE-ELPAAIVYIAGLVDEQLVSQSLLEDL----------IDIPSV 101 Query: 105 XXXRLPAMILSRALEIGEAEIKEEWKEMMLAVLSGDTAILLDGYAASILCATRGGEWRSV 164 + +I + L IG + E+WK + +++G T I +DG +++ +T GGE RS+ Sbjct: 102 QPEQAVCLIYDKTLAIGGVKEAEKWKSLYAGLVAGSTLIFVDGADKALIASTEGGEHRSI 161 Query: 165 SEPTSQLVVRGPKDGFVESVATNISLIRRRIKSSKLRLEYMKIGSETQTLVAMMYVKDIA 224 EP++Q+ RGP+ GF ES+ TNIS+IRR +K+ + +E M+IG ET T VA+MY+ I Sbjct: 162 QEPSTQVSFRGPRQGFTESLQTNISMIRRYVKTPDVWVEKMEIGHETNTDVALMYINGIC 221 Query: 225 GEDLISEVRERLGKIAMNEVLESGYIEELIQDKTFTPFPTIYNTERPDVAAGNLLEGRVV 284 + LI EVR+RL I ++ +LESGYIE+LI+D F+ FPT+Y+TERPDV AGNLLEGR Sbjct: 222 DKKLIKEVRKRLKHIDIDSILESGYIEQLIEDAPFSVFPTVYHTERPDVVAGNLLEGRFA 281 Query: 285 IIVDGTPFVLILPAVFTQFFQSAEDYSQRFDIAILMRVVRYMSFIVLILGPSVYIALTTY 344 IIVDGTPFVLI PA+F QFFQS EDY RFDIA +RV+R + F + ++ P +Y+A TT+ Sbjct: 282 IIVDGTPFVLIAPALFIQFFQSVEDYYSRFDIATSIRVLRVLIFFISLVAPGIYVAATTF 341 Query: 345 HYEMIPTTLLINLLAQRENVPFPALVEALLMEGAFEILREAGVRMPRVIGQTVSVVGALI 404 H EMIPT LLI + AQRE VPFPA++EAL ME AFEILREAGVR+PRV+G VS+VGAL+ Sbjct: 342 HQEMIPTQLLIVIAAQRETVPFPAVIEALTMEVAFEILREAGVRLPRVVGSAVSIVGALV 401 Query: 405 LGSAVVEAGIITPIMVIVVALTGIASFAIPAYNMSIAGRLIRXXXXXXXXXXXXYGVILG 464 +G A V+AGI++P MVIVVALT IASFA PA++M+I+ RLIR YG+ILG Sbjct: 402 IGQAAVQAGIVSPAMVIVVALTAIASFATPAFDMAISARLIRFLFILSAAVMGFYGLILG 461 Query: 465 LIVMVAHMNSLRSFGVPYLSPFVPLSVKGQKDTILRLPLW 504 +I+M H+ LRSFG PY+SP PL+ G KD++LR PLW Sbjct: 462 IIMMFVHLCGLRSFGFPYMSPLAPLTPGGLKDSLLRAPLW 501 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,869,240,346 Number of extensions: 109434974 Number of successful extensions: 281720 Number of sequences better than 1.0e-05: 1091 Number of HSP's gapped: 280806 Number of HSP's successfully gapped: 1091 Length of query: 555 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 414 Effective length of database: 1,732,333,088 Effective search space: 717185898432 Effective search space used: 717185898432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)