BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0054.p (386 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KZH6.1 Q5KZH6_GEOKA Spore germination protein 215 5e-54 C3IZT9.1 C3IZT9_9BACI Germination protein, Ger(X)C family 211 1e-52 B7YCV1.1 B7YCV1_9BACI Germination protein, Ger(X)C family 211 1e-52 A3F902.1 A3F902_BACME GerUC 209 5e-52 B7GJD2.1 B7GJD2_ANOFW Spore germination protein KC 206 4e-51 >Q5KZH6.1 Q5KZH6_GEOKA Spore germination protein Length = 402 Score = 215 bits (548), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 118/385 (30%), Positives = 215/385 (55%), Gaps = 10/385 (2%) Query: 6 VLTGCWDSVELNRRAIVSGVAIDRGPSEDQKYVLSFQVIVAEEISGEQTRSNS----PVA 61 +L+GCW EL A + + +D+ +ED KY+++FQV+ ++G R PV+ Sbjct: 17 LLSGCWSQKELTDLAFIVAIGLDK--TEDGKYLVTFQVVNPGNVAGATQRGGGAAGVPVS 74 Query: 62 LYSGSGRTMFEALANASRQTARFLSLGHIRVLVISEKFAREGIKDMLDVLERESDMRLTS 121 +Y+ +G T+ EA AS + +R H ++V+ E+ AREG+ +LD +ER + +R T+ Sbjct: 75 IYTSTGDTLVEASRKASEKLSRLPYYAHTNLIVVGEEVAREGLSGVLDAMERGNQLRPTA 134 Query: 122 LVFISKDQPASEVLSTMTVFSKIPANDLVEKLDTTSKQFGYNYKMEVDDVIRGIQVRGGG 181 + I++ A +VL+ +T KI AN +++ ++ +SK +G +++ I + G Sbjct: 135 HLVIARTYLAKDVLTVLTPIDKISANQIIKTIEFSSKNWGETVHVDIWGAIHCLATAGRN 194 Query: 182 PVINGVFTTGNRDRANSNDNLKTITPDTILRISRLAVFKDDKLKGWVEGKTAFGTVLLLN 241 VI GV G+ R +N+++ PD +++ LA+FK+D+L WV+G ++ G + LL+ Sbjct: 195 LVITGVTVEGDLKRGKRQENVQSSVPDARIKLEGLALFKNDRLVDWVDGPSSRGVLWLLD 254 Query: 242 KLKE--FPVVIKKDNGYLAFNVYQSQVSVKAEAKDPEHPVILIKITQQAALKESPNSLDL 299 +++ V K + + V +++ VKA+ KD PVI + I + + E+ +DL Sbjct: 255 CIRQTNLTVSWKGKKEAINYQVVRAKTDVKAKVKDG-RPVISVHIQAEGDVIETRVPIDL 313 Query: 300 TSPEVLEKLSGQLTKEARSQIEGAVAAARKLESDYIGFGEAVERENPHGWKKIKDHWED- 358 + +L +L ++ K + ++E A+ A++ ++D GFGEAV R +P WKKI+ W D Sbjct: 314 SDASMLFQLEKEIKKNLKQEVEEAIETAQRDKTDVFGFGEAVHRTDPRLWKKIESKWHDR 373 Query: 359 IFAACEIKIDADTVIRHTDMRGNSF 383 F ++ + AD IR T +R + Sbjct: 374 YFPELDVTVTADMYIRRTGLRNKPY 398 >C3IZT9.1 C3IZT9_9BACI Germination protein, Ger(X)C family Length = 401 Score = 211 bits (536), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 120/390 (30%), Positives = 205/390 (52%), Gaps = 10/390 (2%) Query: 1 VLLPLVLTGCWDSVELNRRAIVSGVAIDRGPSEDQKYVLSFQVIVAEEISGEQTRSNS-- 58 V+ +L GCW EL A + V +D+ ++D KY+ SFQV+ ++G R Sbjct: 12 VVCTGMLAGCWSKKELTDLAFIIAVGLDK--TKDGKYLASFQVVNPGNVAGATQRGGGAA 69 Query: 59 --PVALYSGSGRTMFEALANASRQTARFLSLGHIRVLVISEKFAREGIKDMLDVLERESD 116 PV++Y+ +G T+ EA AS + +R L H ++VI E+ AREG+ + D LER S Sbjct: 70 GVPVSIYTSTGDTLVEASRKASEKMSRLLYYAHTNLMVIGEEVAREGLDGVFDALERNSQ 129 Query: 117 MRLTSLVFISKDQPASEVLSTMTVFSKIPANDLVEKLDTTSKQFGYNYKMEVDDVIRGIQ 176 R T+ + I+ A E+L+ +T KI AN +++ L+ + +G ++V VIR + Sbjct: 130 FRTTAHLVIAHGHSAKEILTVLTPVDKISANQIIKTLEFSEMAWGQTMNVDVWQVIRDLA 189 Query: 177 VRGGGPVINGVFTTGNRDRANSNDNLKTITPDTILRISRLAVFKDDKLKGWVEGKTAFGT 236 G PVI GV GN R N+++ PD + + LA+FK+ +L WV G TA G Sbjct: 190 SPGRNPVITGVRIEGNPARGKRQTNVQSSVPDARVSLDGLALFKNGRLVKWVNGPTARGV 249 Query: 237 VLLLNKLKEFPVVI--KKDNGYLAFNVYQSQVSVKAEAKDPEHPVILIKITQQAALKESP 294 V +L+++K+ V I + + + +++ V+A K P + + I + + E+ Sbjct: 250 VWVLDRIKQTNVTIPWNEKQEAIGYRTVRAKTDVEATVKRG-RPTVSVHIKAEGDISEAL 308 Query: 295 NSLDLTSPEVLEKLSGQLTKEARSQIEGAVAAARKLESDYIGFGEAVERENPHGWKKIKD 354 + L VL +L ++ ++ + +++ A+ A++ +D GFG+AV R P WKKI++ Sbjct: 309 VPIHLDDANVLFQLEKEIERDIKKEVKQAIETAQRARTDVFGFGDAVHRAAPRLWKKIQN 368 Query: 355 HWED-IFAACEIKIDADTVIRHTDMRGNSF 383 W D F ++ + AD IR +R + Sbjct: 369 EWHDRYFPELDVTVTADIYIRRIGLRNKPY 398 >B7YCV1.1 B7YCV1_9BACI Germination protein, Ger(X)C family Length = 401 Score = 211 bits (536), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 120/390 (30%), Positives = 205/390 (52%), Gaps = 10/390 (2%) Query: 1 VLLPLVLTGCWDSVELNRRAIVSGVAIDRGPSEDQKYVLSFQVIVAEEISGEQTRSNS-- 58 V+ +L GCW EL A + V +D+ ++D KY+ SFQV+ ++G R Sbjct: 12 VVCTGMLAGCWSKKELTDLAFIIAVGLDK--TKDGKYLASFQVVNPGNVAGATQRGGGAA 69 Query: 59 --PVALYSGSGRTMFEALANASRQTARFLSLGHIRVLVISEKFAREGIKDMLDVLERESD 116 PV++Y+ +G T+ EA AS + +R L H ++VI E+ AREG+ + D LER S Sbjct: 70 GVPVSIYTSTGDTLVEASRKASEKMSRLLYYAHTNLMVIGEEVAREGLDGVFDALERNSQ 129 Query: 117 MRLTSLVFISKDQPASEVLSTMTVFSKIPANDLVEKLDTTSKQFGYNYKMEVDDVIRGIQ 176 R T+ + I+ A E+L+ +T KI AN +++ L+ + +G ++V VIR + Sbjct: 130 FRTTAHLVIAHGHSAKEILTVLTPVDKISANQIIKTLEFSEMAWGQTMNVDVWQVIRDLA 189 Query: 177 VRGGGPVINGVFTTGNRDRANSNDNLKTITPDTILRISRLAVFKDDKLKGWVEGKTAFGT 236 G PVI GV GN R N+++ PD + + LA+FK+ +L WV G TA G Sbjct: 190 SPGRNPVITGVRIEGNPARGKRQTNVQSSVPDARVSLDGLALFKNGRLVKWVNGPTARGV 249 Query: 237 VLLLNKLKEFPVVI--KKDNGYLAFNVYQSQVSVKAEAKDPEHPVILIKITQQAALKESP 294 V +L+++K+ V I + + + +++ V+A K P + + I + + E+ Sbjct: 250 VWVLDRIKQTNVTIPWNEKQEAIGYRTVRAKTDVEATVKRG-RPTVSVHIKAEGDISEAL 308 Query: 295 NSLDLTSPEVLEKLSGQLTKEARSQIEGAVAAARKLESDYIGFGEAVERENPHGWKKIKD 354 + L VL +L ++ ++ + +++ A+ A++ +D GFG+AV R P WKKI++ Sbjct: 309 VPIHLDDANVLFQLEKEIERDIKKEVKQAIETAQRARTDVFGFGDAVHRAAPRLWKKIQN 368 Query: 355 HWED-IFAACEIKIDADTVIRHTDMRGNSF 383 W D F ++ + AD IR +R + Sbjct: 369 EWHDRYFPELDVTVTADIYIRRIGLRNKPY 398 >A3F902.1 A3F902_BACME GerUC Length = 409 Score = 209 bits (531), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 133/394 (33%), Positives = 224/394 (56%), Gaps = 16/394 (4%) Query: 1 VLLPLVLTGCWDSVELNRRAIVSGVAIDRGPSEDQKYVLSFQVI----VAEEISGEQTRS 56 +L+ +L+ CW EL AIVS V ID+ ++D KY L+ Q+I VA + G + Sbjct: 12 ILVTALLSSCWSKKELTDLAIVSAVGIDK--TKDGKYHLTLQIINPGNVAGGMQGGGGGT 69 Query: 57 NSP-VALYSGSGRTMFEALANASRQTARFLSLGHIRVLVISEKFARE-GIKDMLDVLERE 114 SP V +YS SG + EA AS + +R L H ++VI EK ARE G+ ++DVL+R+ Sbjct: 70 QSPPVTIYSASGDNIVEASRRASSRISRRLYYAHANLVVIGEKLARESGLNPLIDVLDRD 129 Query: 115 SDMRLTSLVFISKDQPASEVLSTMTVFSKIPANDLVEKLDTTSKQFGYNYKMEVDDVIRG 174 + R T+ + I+ A++++ T+T KIPAN +++ L+ T +++G N K+ + DVI G Sbjct: 130 PEFRTTATMVIANHSTAADLVQTLTPVDKIPANKVLKTLEFTERKWGENVKLSIQDVIIG 189 Query: 175 IQVRGGGPVINGVFTTGNRDRANSNDNLKTITPDTILRISRLAVFKDDKLKGWVEGKTAF 234 ++ GG ++ G GN +A +NL+ P+ LR S +AV K KL W+ GK A Sbjct: 190 LESPGGETIVGGFRLVGNPKQARMLENLQESAPEATLRASGIAVLKQGKLVDWLYGKPAR 249 Query: 235 GTVLLLNKLKEFPVVI----KKDNGYLAFNVYQSQVSVKAEAKDPEHPVILIKITQQAAL 290 GTV +L+K++ + I KK+ +A+ + + SV A+ K+ + P I + + + Sbjct: 250 GTVWILDKIQGTDINIDWAGKKE--AIAYQTVRQKTSVSAQVKNGQ-PHISVHTRVEGDI 306 Query: 291 KESPNSLDLTSPEVLEKLSGQLTKEARSQIEGAVAAARKLESDYIGFGEAVERENPHGWK 350 + +D+T+P+V+ K+ + KE + +++ A+ A+K ++D GFGE V R P+ +K Sbjct: 307 GDMEVPVDITNPKVITKIEQFVRKEIKKELKTAIEQAQKNKTDIFGFGEVVHRTRPNQFK 366 Query: 351 KIKDHWEDI-FAACEIKIDADTVIRHTDMRGNSF 383 K+K W D+ F ++ I + +R +R SF Sbjct: 367 KLKTEWNDVYFPKLDVDITVEAYVRRAGLRNKSF 400 >B7GJD2.1 B7GJD2_ANOFW Spore germination protein KC Length = 354 Score = 206 bits (523), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 117/351 (33%), Positives = 195/351 (55%), Gaps = 8/351 (2%) Query: 40 SFQVIVAEEISGEQTRSNS----PVALYSGSGRTMFEALANASRQTARFLSLGHIRVLVI 95 +FQ++ ++G R PVALY+ +G T+ EA AS+Q +R H ++VI Sbjct: 3 TFQIVNPGNVAGATQRGGGSAGLPVALYTSTGDTLVEASRKASKQISRIPYYSHTNLVVI 62 Query: 96 SEKFAREGIKDMLDVLERESDMRLTSLVFISKDQPASEVLSTMTVFSKIPANDLVEKLDT 155 E+ A+EGI+ +LD +ER R + + I++ Q A ++LS +T KIPAN +++ L Sbjct: 63 GEELAKEGIQQILDAVERNPQFRPAANIIIARKQTAKQLLSVLTPIDKIPANQIIKTLQF 122 Query: 156 TSKQFGYNYKMEVDDVIRGIQVRGGGPVINGVFTTGNRDRANSNDNLKTITPDTILRISR 215 T G N + ++I + G VI+ G+ + + DN++TI P LR + Sbjct: 123 TKDLLGENVDTRIREIIENFSLDGKEVVISSFRIVGDWQKGDEQDNVRTIEPSARLRAAE 182 Query: 216 LAVFKDDKLKGWVEGKTAFGTVLLLNKLKEFPVVIK--KDNGYLAFNVYQSQVSVKAEAK 273 LAVFK+ KL GW++ + A G + +LNK+K+ V I + L++ V ++Q V A+ K Sbjct: 183 LAVFKNGKLVGWMKNEEARGVLWVLNKVKQTSVNIDWGESKEGLSYKVMRAQAKVTAKLK 242 Query: 274 DPEHPVILIKITQQAALKESPNSLDLTSPEVLEKLSGQLTKEARSQIEGAVAAARKLESD 333 D +P+I + I + + E+ S+D T P+ + +L ++ +E + +++ AV A+K +SD Sbjct: 243 DG-NPIISVFIKTEGDISETFVSIDFTDPKQIIELEKKIQQEIKKEVKEAVHTAQKKKSD 301 Query: 334 YIGFGEAVERENPHGWKKIKDHWED-IFAACEIKIDADTVIRHTDMRGNSF 383 GFGE V R P WK++K W + F EI + D+ IR T +R S+ Sbjct: 302 IFGFGEVVHRTYPEEWKEMKKLWHNKYFPKLEIHVKVDSFIRRTGLRTRSY 352 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,141,927,616 Number of extensions: 85486432 Number of successful extensions: 239523 Number of sequences better than 1.0e-05: 270 Number of HSP's gapped: 239403 Number of HSP's successfully gapped: 289 Length of query: 386 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 249 Effective length of database: 1,770,017,148 Effective search space: 440734269852 Effective search space used: 440734269852 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)