BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0053.p (366 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3F903.1 A3F903_BACME GerUB 227 1e-57 B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB 223 2e-56 A3F904.1 A3F904_BACME GerVB 220 1e-55 A4IND9.1 A4IND9_GEOTN Spore germination protein GerKB 219 5e-55 B4BK03.1 B4BK03_9BACI Spore germination protein 219 5e-55 >A3F903.1 A3F903_BACME GerUB Length = 366 Score = 227 bits (579), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 137/363 (37%), Positives = 201/363 (55%), Gaps = 8/363 (2%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M KI Q F + +LFELG+AL+V L + A +DAW +F Y LY Sbjct: 1 MEKAKISASQLFILMVLFELGSALLVPLAIRAKQDAWLAILLGMIFSFVLFFVYHKLYTY 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP + T Y +K+LGK +GT +A +YI+ ++ A+R LRD ML +TPLF Sbjct: 61 YPNLLPTEYMQKILGKVLGTILAFIYILYFMYDASRVLRDFGEMLLTFAYPDTPLFIANA 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y + KG E +++ SG I + L PVLE+G P Sbjct: 121 LLMLVIIYTIRKGIEVIARSGELLFIFMYILAVAGFILIVSSGLIEIKNLQPVLEEGLLP 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 ++ L + FPF E I FTM++P+L++ KK + A +S + L+ TM +N+SVLG Sbjct: 181 SLKVALSETLYFPFTEAIVFTMILPYLNDSKKAKVTMLCATGLSGINLAITMLINVSVLG 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 ++ RS PL+ T+ I ++DF++R+D+F ++ ++IG+FFK+SV F AA+IG ++LF I Sbjct: 241 VNLTARSQFPLLSTVESIQVADFLERLDVFFMLAMVIGIFFKVSVLFYAAVIGTANLFKI 300 Query: 301 PY-RRMLYPCTLIILFTSMLDARSFTEHLDEGGRLLYTVYPFLMI----AIPAVLILVSV 355 RM YP +IILF SM A +F EHL EG LY L I IP++L+LVS Sbjct: 301 KSPSRMSYPLGIIILFMSMTIASNFQEHLHEG---LYAAKFVLHIPLFTVIPSLLLLVSF 357 Query: 356 VKS 358 +K+ Sbjct: 358 LKN 360 >B7GJD3.1 B7GJD3_ANOFW Spore germination protein KB Length = 370 Score = 223 bits (569), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 123/352 (34%), Positives = 204/352 (57%), Gaps = 4/352 (1%) Query: 10 QFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRKYPGMSFTAY 69 Q F + +LFE G+A+V+ LG+ A +D W + Y L+R YP + T+Y Sbjct: 10 QLFMLAILFEHGSAMVIPLGVDAKQDVWLAILCGMVLGGGLLFVYYRLFRYYPRLPLTSY 69 Query: 70 TRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXXXXXXXXAYV 129 +++++G + G + L+Y++ ++ +AAR LRD +L TPLF Y Sbjct: 70 SQRIVGSFFGRVLGLIYVLYFLYIAARVLRDFGELLLAFAYSETPLFILNAVMMLTVMYA 129 Query: 130 LHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEPVIRSVLHQN 189 ++KG E +++ +G+++L+RL P+LE+G P+ V Q Sbjct: 130 VYKGIEVIARASQLFFFSLYWILILGFVLIIITGAVDLHRLQPILEEGWGPIFHVVFTQT 189 Query: 190 YMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLGADIVERSPL 249 PFGEMI FTM+ P+L +K A+ +S + L+ MA+NI+VLGADIV RS Sbjct: 190 LYVPFGEMIVFTMIFPYLQKQEKVMKAGFLALFLSGINLAIIMAMNIAVLGADIVSRSTF 249 Query: 250 PLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI-PYRRMLYP 308 PL+ TI +I +++F++R+D+ ++ LI+G FFK+ VFF A +IG +DLF I Y+R++YP Sbjct: 250 PLLNTIQEIRVANFLERLDVLFMIALIVGGFFKVGVFFYAGVIGAADLFQIKEYQRLVYP 309 Query: 309 CTLIILFTSMLDARSFTEHLDEGGRLLYTVYPF--LMIAIPAVLILVSVVKS 358 + +LF S+ A +F EH+ EG +++ T+Y L I IP +L+L+++++ Sbjct: 310 LGISVLFLSIAIASNFAEHIKEGLQIV-TIYMHIPLQIVIPVLLLLIAMLRH 360 >A3F904.1 A3F904_BACME GerVB Length = 366 Score = 220 bits (561), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 130/360 (36%), Positives = 198/360 (55%), Gaps = 2/360 (0%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M KI Q F + +LFELG++L+V + M A +DAW +F Y L+ Sbjct: 1 MEKAKISASQLFILMVLFELGSSLLVPIAMDAKQDAWLAILIGMVGSFVLFLVYHKLHSY 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP + T Y +K++GK IGT +A VYI+ ++ AAR LRD ML + TPLF Sbjct: 61 YPDLLPTEYMQKIMGKGIGTVLAFVYILYFMYDAARVLRDFGAMLLTSAYPETPLFIAHT 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y + KG E +++ SG I L PVLE+G P Sbjct: 121 LLMLVVIYTIRKGIEVVARSGEILFIFIYIFAIMGFVLIVCSGLIEFTNLQPVLEEGVLP 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 V++ Q FPF E + FTM++P+L + KK + A +S + L+ TM +NISVLG Sbjct: 181 VLKVAFTQTIYFPFAEAMVFTMILPYLKDQKKAKMTMLCATGLSGINLTITMLINISVLG 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 D+ RS PL+ T+ I ++DF++R+D+F ++ L+IG F KISV AA+IG ++LF I Sbjct: 241 VDLTSRSQFPLLSTVQSIQVADFLERLDVFFMLALVIGGFIKISVLLYAAVIGTANLFKI 300 Query: 301 PY-RRMLYPCTLIILFTSMLDARSFTEHLDEGGRL-LYTVYPFLMIAIPAVLILVSVVKS 358 R+ YP +ILF + A +F EHL EG ++ ++ ++ ++ IP++L+LV+ +K+ Sbjct: 301 KSPSRLSYPLGFVILFMASTIASNFQEHLHEGLKVEMFILHMPILAIIPSLLLLVAFLKN 360 >A4IND9.1 A4IND9_GEOTN Spore germination protein GerKB Length = 367 Score = 219 bits (557), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 123/363 (33%), Positives = 207/363 (57%), Gaps = 2/363 (0%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M + KI + Q F + +LFE G+A+V+ LG++A +D W +F Y L+ Sbjct: 1 MESSKINVRQLFVLIVLFEHGSAVVIPLGVSAKQDVWISILLSLAIGLLLFFVYRRLFEY 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP TAY +++G +G +A+VYI ++ +AAR LRD +L TPLF Sbjct: 61 YPDQPLTAYLPQIVGAPLGKLLAIVYITYFLYIAARVLRDFGELLLTFAYPETPLFVLNA 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y ++KG E ++ SG +++ +L P+LE+G Sbjct: 121 IMAVTVMYGVYKGIEVLARTGELFLIVLYFLAIIGFVLVFVSGIVDVTQLQPMLEEGWRR 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 V ++V + PFGEMI FTML P+++ K M++S + L+ MA+N++VLG Sbjct: 181 VWKTVFTETLYVPFGEMIVFTMLFPYINTPTKVGRAGVVGMVLSGINLALIMAINVAVLG 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 D V RS PL+ TI ++ ++DF++R+D+F ++ LI G FFKIS+FF A ++G ++LFGI Sbjct: 241 PDTVSRSSFPLLDTIRRVQVADFLERLDVFFMITLITGGFFKISIFFYAGIVGAANLFGI 300 Query: 301 -PYRRMLYPCTLIILFTSMLDARSFTEHLDEGGRLL-YTVYPFLMIAIPAVLILVSVVKS 358 YRR++YP L+IL S++ A ++ EH+ EG +++ + ++ L + IP +L++++ ++ Sbjct: 301 SSYRRLVYPLGLLILILSVVIASNYAEHIQEGLKIVTFYLHVPLQVIIPVLLLIIAAIRR 360 Query: 359 YFS 361 F+ Sbjct: 361 RFN 363 >B4BK03.1 B4BK03_9BACI Spore germination protein Length = 367 Score = 219 bits (557), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 123/363 (33%), Positives = 207/363 (57%), Gaps = 2/363 (0%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M + KI + Q F + +LFE G+A+V+ LG++A +D W +F Y L+ Sbjct: 1 MESSKINVRQLFVLIVLFEHGSAVVIPLGVSAKQDVWISILLSLAIGLLLFFVYRRLFEY 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP TAY +++G +G +A+VYI ++ +AAR LRD +L TPLF Sbjct: 61 YPDQPLTAYLPQIVGAPLGKLLAIVYITYFLYIAARVLRDFGELLLTFAYPETPLFVLNA 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y ++KG E ++ SG +++ +L P+LE+G Sbjct: 121 IMAVTVMYGVYKGIEVLARTGELFLIVLYFLAIIGFVLVFVSGIVDVTQLQPMLEEGWRR 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 V ++V + PFGEMI FTML P+++ K M++S + L+ MA+N++VLG Sbjct: 181 VWKTVFTETLYVPFGEMIVFTMLFPYINTPTKVGRAGVVGMVLSGINLALIMAINVAVLG 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 D V RS PL+ TI ++ ++DF++R+D+F ++ LI G FFKIS+FF A ++G ++LFGI Sbjct: 241 PDTVSRSSFPLLDTIRRVQVADFLERLDVFFMITLITGGFFKISIFFYAGIVGAANLFGI 300 Query: 301 -PYRRMLYPCTLIILFTSMLDARSFTEHLDEGGRLL-YTVYPFLMIAIPAVLILVSVVKS 358 YRR++YP L+IL S++ A ++ EH+ EG +++ + ++ L + IP +L++++ ++ Sbjct: 301 SSYRRLVYPLGLLILILSVVIASNYAEHIQEGLKIVTFYLHVPLQVIIPVLLLIIAAIRR 360 Query: 359 YFS 361 F+ Sbjct: 361 RFN 363 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.144 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,685,728,629 Number of extensions: 59123944 Number of successful extensions: 192384 Number of sequences better than 1.0e-05: 179 Number of HSP's gapped: 192224 Number of HSP's successfully gapped: 179 Length of query: 366 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 229 Effective length of database: 1,770,017,148 Effective search space: 405333926892 Effective search space used: 405333926892 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)