BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0051.p (605 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ24.1 B3KJ24_9BACI Methylenetetrahydrofolate reductase EC=1.5... 976 0.0 B1DHM7.1 B1DHM7_9BACL Methylenetetrahydrofolate reductase EC=1.5... 819 0.0 C0ZBM2.1 C0ZBM2_BREBN Putative 5,10-methylenetetrahydrofolate re... 650 0.0 A9U7N9.1 A9U7N9_PHYPA Methylenetetrahydrofolate reductase EC=1.5... 614 e-174 C5D741.1 C5D741_9BACI Homocysteine S-methyltransferase 598 e-169 >B3KJ24.1 B3KJ24_9BACI Methylenetetrahydrofolate reductase EC=1.5.1.20 Length = 623 Score = 976 bits (2523), Expect = 0.0, Method: Compositional matrix adjust. Identities = 469/605 (77%), Positives = 527/605 (87%), Gaps = 3/605 (0%) Query: 1 MGTFLYQKGFPVGISYEELNLTSPEVIEDVHRSYIGAGAVLLESNTYSANYDKLSKFGLE 60 MGT+LYQKGFPVGISYEELNLTSPEVI +VHR Y+ AGA ++E+NT+SANYDKLSK+GLE Sbjct: 20 MGTYLYQKGFPVGISYEELNLTSPEVIGNVHRQYVEAGAQVIETNTFSANYDKLSKYGLE 79 Query: 61 AKVGDINXXXXXXXXXXXXESGYVVGAVGSIRAGKRANLSGTELKRFYTEQFSALLEEQP 120 A+VG+IN + GYVVGAVGSIRAGKR+N+S +ELKR++ EQ S L+ E Sbjct: 80 ARVGEINRAGVRIAREAAGDQGYVVGAVGSIRAGKRSNISTSELKRYFEEQLSVLISEGV 139 Query: 121 DGILLETFYDVEELHLALRSVRKLSDLPVICQLAVDETARTLDGLTLPEAFHILEQDGAD 180 DGILLETFYDV+E+ LAL R LSDLPVICQ AV++ ARTLDG T+P+A+ IL +GAD Sbjct: 140 DGILLETFYDVDEMQLALAQARMLSDLPVICQFAVEDIARTLDGFTMPDAYRILSHEGAD 199 Query: 181 VIGFNCRTGPNGIKRALKSLQGNLKLPASIYPNAGIADYVDGQYRYGASPEYFGQMAPVF 240 VIGFNCRTGPNGI RAL++L G + LPAS+YPNAGIADYVDG+YRYGASPEYFG+ A F Sbjct: 200 VIGFNCRTGPNGIMRALETLNGIMNLPASVYPNAGIADYVDGEYRYGASPEYFGKTALEF 259 Query: 241 ADMGARIIGGCCGTTPQHIAEISAALRGYTPQPLPEPTGQRDPERLIVQEHLAED-EERD 299 AD GARIIGGCCGTTP+HIAEIS+AL GY P PLP + + R+++ E E ER Sbjct: 260 ADRGARIIGGCCGTTPEHIAEISSALSGYVPSPLP-AVEEAEINRIVIHETAQEQFYERS 318 Query: 300 GREPTLVDLVKERHTVIVELDPPRDLDIAKFMRGAEALRKAGADALTLADNSLAVTRMSN 359 G EPT+VD+VKERHTVIVELDPPRDLDI KFM+GAEAL+KAGADALTLADNSLAVTRMSN Sbjct: 319 G-EPTIVDIVKERHTVIVELDPPRDLDITKFMKGAEALKKAGADALTLADNSLAVTRMSN 377 Query: 360 MALGHLVQARTGLRPLVHIACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARFGDLPGS 419 MALGHLVQARTGLRPL+HIACRDRNLIGTQSH+MGFDALGIDHVLAVTGDPARFGDLP S Sbjct: 378 MALGHLVQARTGLRPLIHIACRDRNLIGTQSHMMGFDALGIDHVLAVTGDPARFGDLPDS 437 Query: 420 SSIYDLTSFEIIRMIKQLNDGIAFSGKPLKQNANFVIGAAFNPNVKHLDKAVERLEKKIA 479 SS+YD+TSFEIIRMIKQLNDGIAFSGKPLKQ A FV+GAAFNPNV+HLDKAV RLEKKIA Sbjct: 438 SSVYDMTSFEIIRMIKQLNDGIAFSGKPLKQKAKFVVGAAFNPNVRHLDKAVARLEKKIA 497 Query: 480 SGADYIMTQPVYDPELIARIAKATEHLEIPIFIGIMPLASGRNAEYLHNEVPGIQLSPEV 539 SGADY+MTQPVYDPEL+ R+ +AT HL+IPIFIGIMPLASGRNAEYLHNEVPGIQLS EV Sbjct: 498 SGADYVMTQPVYDPELMVRLREATAHLDIPIFIGIMPLASGRNAEYLHNEVPGIQLSDEV 557 Query: 540 RSRMQGLEGEAGRAEGVRIAKELLDAATAHFNGIYLMTPFMFYDMSVQLLEYVWAKQGRK 599 RSRM GLEGEAGRA GV+I KELLD AT HFNGIYLMTPFMFY+MSVQL+EYVW K GR+ Sbjct: 558 RSRMAGLEGEAGRAMGVKIGKELLDVATEHFNGIYLMTPFMFYEMSVQLMEYVWEKSGRR 617 Query: 600 LSPLF 604 L+PLF Sbjct: 618 LTPLF 622 >B1DHM7.1 B1DHM7_9BACL Methylenetetrahydrofolate reductase EC=1.5.1.20 Length = 632 Score = 819 bits (2116), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/609 (66%), Positives = 470/609 (77%), Gaps = 12/609 (1%) Query: 1 MGTFLYQKGFPVGISYEELNLTSPEVIEDVHRSYIGAGAVLLESNTYSANYDKLSKFGLE 60 MGT+LYQ GFPVG+SYEELN+T P+VIE VHR Y AGA ++E+NT+SAN KLSK+GLE Sbjct: 20 MGTYLYQMGFPVGVSYEELNMTRPDVIEGVHRRYYEAGARVIETNTFSANLVKLSKYGLE 79 Query: 61 AKVGDINXXXXXXXXXXXXESGYVVGAVGSIRAGKRANLSGTELKRFYTEQFSALLEEQP 120 + IN E YVVGAVGSIRAGK ++ +E+ + Q ALL E Sbjct: 80 PDMEAINRAGVRIAKAAAGEDAYVVGAVGSIRAGKLKDVRTSEVSHAFERQLDALLVEGV 139 Query: 121 DGILLETFYDVEELHLALRSVRKLSDLPVICQLAVDETARTLDGLTLPEAFHILEQDGAD 180 DGIL ETFYD +E+ LAL RKL+ LPVICQ A + RT DG L +AF +L+ +GAD Sbjct: 140 DGILFETFYDFDEMRLALGLARKLTQLPVICQFAAQDVGRTQDGTGLLDAFTLLKDEGAD 199 Query: 181 VIGFNCRTGPNGIKRALKSLQGNLKLPASIYPNAGIADYVDGQYRYGASPEYFGQMAPVF 240 V+GFNCR+GPNGI RA++S+ G ++LP SI+PNAGI DYVDG+Y+Y A P+YF + A F Sbjct: 200 VVGFNCRSGPNGIMRAVESIGGPVELPLSIFPNAGIPDYVDGKYKYSAGPDYFAETALQF 259 Query: 241 ADMGARIIGGCCGTTPQHIAEISAALRGYTPQPLPEPTGQRDP---ERLIVQEHLAE--- 294 AD GARIIGGCCGTTP+HIA ++ AL+ Y PQ D E ++++ AE Sbjct: 260 ADRGARIIGGCCGTTPEHIAAMAKALKDYKPQNGAAAVAIADSPEAEIIVIEAEPAEVVK 319 Query: 295 ---DEERD---GREPTLVDLVKERHTVIVELDPPRDLDIAKFMRGAEALRKAGADALTLA 348 E D EP+LVD V+ RH VIVELDPPRDLDI KFM GA A++ GADA+T+A Sbjct: 320 NASGERADTPINSEPSLVDTVRVRHAVIVELDPPRDLDITKFMAGAAAIKACGADAITMA 379 Query: 349 DNSLAVTRMSNMALGHLVQARTGLRPLVHIACRDRNLIGTQSHLMGFDALGIDHVLAVTG 408 DNSLAVTRMSN+AL LV+ R GLRPLVHIACRDRNLIGTQSHLMG DALGIDHVLAVTG Sbjct: 380 DNSLAVTRMSNLALAALVKERIGLRPLVHIACRDRNLIGTQSHLMGLDALGIDHVLAVTG 439 Query: 409 DPARFGDLPGSSSIYDLTSFEIIRMIKQLNDGIAFSGKPLKQNANFVIGAAFNPNVKHLD 468 DPARFGDLP SSS+YDLTSFE+IRM KQLNDGIAFSGKPLKQ A FV+GAAFNPNVKHLD Sbjct: 440 DPARFGDLPDSSSVYDLTSFEMIRMTKQLNDGIAFSGKPLKQKAKFVVGAAFNPNVKHLD 499 Query: 469 KAVERLEKKIASGADYIMTQPVYDPELIARIAKATEHLEIPIFIGIMPLASGRNAEYLHN 528 KAV+RLEKKIASGADYIMTQPVYDP LI +IA+AT+HL +P+FIGIMPLASGRNAEYLHN Sbjct: 500 KAVQRLEKKIASGADYIMTQPVYDPVLIEKIAEATKHLPVPVFIGIMPLASGRNAEYLHN 559 Query: 529 EVPGIQLSPEVRSRMQGLEGEAGRAEGVRIAKELLDAATAHFNGIYLMTPFMFYDMSVQL 588 EVPGIQLS EVR RM GLEG GRAEGVRI++ELLD A HF GIY MTPFMFY+M+ +L Sbjct: 560 EVPGIQLSDEVRKRMAGLEGAEGRAEGVRISRELLDTAIRHFKGIYFMTPFMFYEMAAEL 619 Query: 589 LEYVWAKQG 597 +YVWAK G Sbjct: 620 TDYVWAKTG 628 >C0ZBM2.1 C0ZBM2_BREBN Putative 5,10-methylenetetrahydrofolate reductase EC=1.5.1.20 Length = 621 Score = 650 bits (1678), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/612 (53%), Positives = 424/612 (69%), Gaps = 24/612 (3%) Query: 1 MGTFLYQKGFPVGISYEELNLTSPEVIEDVHRSYIGAGAVLLESNTYSANYDKLSKFGLE 60 M T L+ G PVG+S+EEL L++P ++ +VH SY AGA LE+NTYSAN D LS++GLE Sbjct: 23 MATQLHGLGVPVGVSFEELCLSNPRLVHEVHTSYYQAGARFLETNTYSANRDALSRYGLE 82 Query: 61 AKVGDINXXXXXXXXXXXXESGYVVGAVGSIRAGKRANLSGTELKRFYTEQFSALLEEQP 120 KV IN + YV G++GSI AG+ E + Y EQ ALL Sbjct: 83 HKVARINRLAVAIAREAAQDDAYVAGSIGSILAGRVKKKVLDEYRDQYEEQAIALLHAGV 142 Query: 121 DGILLETFYDVEELHLALRSVRKLSDLPVICQLAVDETARTLDGLTLPEAFHILEQDGAD 180 DG++LETF D+EEL LA+ +R L+D+PVI QLA E RT DG L +AF L+ GAD Sbjct: 143 DGLILETFLDLEELLLAIEVIRPLTDVPVIAQLATLEVGRTRDGYALTDAFSELKAAGAD 202 Query: 181 VIGFNCRTGPNGIKRALKSLQGNLKLPA----SIYPNAGIADYVDGQYRYGASPEYFGQM 236 V+G NCR GP + R+ + N +P S++PNAG DG+Y + +SPEYFGQ Sbjct: 203 VVGLNCRLGPAELLRSFE----NTVIPEDALLSVFPNAGRLGMTDGEYAFKSSPEYFGQS 258 Query: 237 APVFADMGARIIGGCCGTTPQHIAEISAALRGYTPQPLPEPT---GQRDPERLIVQEHLA 293 A + G R+IGGCCGTTP H+ I+ AL PQ PT G R ++ Sbjct: 259 ALRLREQGVRLIGGCCGTTPAHVKAIADALESLEPQARVNPTIVAGDRPT--------IS 310 Query: 294 EDEERDGREPTLVDLVKERHTVIVELDPPRDLDIAKFMRGAEALRKAGADALTLADNSLA 353 R+ +P++V+ VK T+IVE DPPRDLD +F+ G L KAGADA+TLADNSLA Sbjct: 311 VQNTREREKPSIVERVKTATTIIVEFDPPRDLDADQFLHGCCELHKAGADAITLADNSLA 370 Query: 354 VTRMSNMALGHLVQARTGLRPLVHIACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARF 413 RMSNMALG L+++R G+ PL+H+ACRDRNLIG QSHLMG +ALGID +L +TGDP+RF Sbjct: 371 TVRMSNMALGALMKSRHGIDPLLHLACRDRNLIGQQSHLMGLNALGIDQILVITGDPSRF 430 Query: 414 GDLPGSSSIYDLTSFEIIRMIKQLNDGIAFSGKPLKQNANFVIGAAFNPNVKHLDKAVER 473 GDLPG+SS++D+TSF++IRM+KQLN+G++FSG+PLKQ A F++GAAFNPNV+++D AV R Sbjct: 431 GDLPGASSVFDVTSFDLIRMVKQLNEGVSFSGRPLKQKAQFIVGAAFNPNVRNMDAAVAR 490 Query: 474 LEKKIASGADYIMTQPVYDPELIARIAKATEHLEIPIFIGIMPLASGRNAEYLHNEVPGI 533 LEKK+ +GADYIMTQPVYD E I + +AT+H+ IP+FIGIMPL S RNAE+LHNEVPGI Sbjct: 491 LEKKVEAGADYIMTQPVYDAESIRNVYEATKHIGIPVFIGIMPLTSSRNAEFLHNEVPGI 550 Query: 534 QLSPEVRSRMQGLEGEAGRAEGVRIAKELLDAATAHFNGIYLMTPFMFYDMSVQLLEYVW 593 +LS E RM+ ++GEA R EG+ IAKEL+D A +FNGIYL+TPF +Y+M+ +L++Y Sbjct: 551 KLSTEALERMKKVQGEAARQEGIAIAKELVDTAVRYFNGIYLITPFNYYEMAAELIQYT- 609 Query: 594 AKQGRKLSPLFR 605 R+ S L R Sbjct: 610 ----RQQSSLVR 617 >A9U7N9.1 A9U7N9_PHYPA Methylenetetrahydrofolate reductase EC=1.5.1.20 (Fragment) Length = 401 Score = 614 bits (1584), Expect = e-174, Method: Compositional matrix adjust. Identities = 300/393 (76%), Positives = 339/393 (86%), Gaps = 2/393 (0%) Query: 80 ESGYVVGAVGSIRAGKRANLSGTELKRFYTEQFSALLEEQPDGILLETFYDVEELHLALR 139 +GYVVGA+GSIR GKR N+S ELK+++ +Q ALL+E DGILLETFYDVEEL+LAL Sbjct: 9 SAGYVVGAIGSIRGGKRTNVSTAELKKYFEQQIQALLQEGVDGILLETFYDVEELYLALS 68 Query: 140 SVRKLSDLPVICQLAVDETARTLDGLTLPEAFHILEQDGADVIGFNCRTGPNGIKRALKS 199 VRKLS LPVICQ AV++ ARTLDG T+PEAF +++Q GADVIGFNCRTGPNGI A+K+ Sbjct: 69 IVRKLSPLPVICQFAVEDVARTLDGYTMPEAFRMMQQQGADVIGFNCRTGPNGIMSAMKT 128 Query: 200 LQGNLKLPASIYPNAGIADYVDGQYRYGASPEYFGQMAPVFADMGARIIGGCCGTTPQHI 259 L G++ +P S+YPNAG+ADYVDG+Y+YGA P+YFGQMA FAD+GARIIGGCCGTTP HI Sbjct: 129 LGGHMSVPMSVYPNAGVADYVDGEYQYGAKPDYFGQMAVKFADLGARIIGGCCGTTPAHI 188 Query: 260 AEISAALRGYTPQPLPEPTGQRDPERLIVQEHLA--EDEERDGREPTLVDLVKERHTVIV 317 A IS+AL+GY PQP+ P + + EHLA D E +G P LVDLVKERHTVIV Sbjct: 189 AAISSALQGYEPQPITYGPIDIAPAPIEIHEHLAPDGDGEGEGGSPNLVDLVKERHTVIV 248 Query: 318 ELDPPRDLDIAKFMRGAEALRKAGADALTLADNSLAVTRMSNMALGHLVQARTGLRPLVH 377 ELDPPRDLDIAKFM+GA+ L+ AGADALTLADNSLAVTRMSNMALGHLVQA TGLRPLVH Sbjct: 249 ELDPPRDLDIAKFMKGAQVLKDAGADALTLADNSLAVTRMSNMALGHLVQAETGLRPLVH 308 Query: 378 IACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARFGDLPGSSSIYDLTSFEIIRMIKQL 437 IACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARFGDLPGSSS+YDLTSFEIIRMIKQL Sbjct: 309 IACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARFGDLPGSSSVYDLTSFEIIRMIKQL 368 Query: 438 NDGIAFSGKPLKQNANFVIGAAFNPNVKHLDKA 470 N+GIAFSGKPLKQ ANFV+GAAFNP+ KHLDKA Sbjct: 369 NEGIAFSGKPLKQKANFVVGAAFNPHGKHLDKA 401 >C5D741.1 C5D741_9BACI Homocysteine S-methyltransferase Length = 616 Score = 598 bits (1543), Expect = e-169, Method: Compositional matrix adjust. Identities = 302/600 (50%), Positives = 404/600 (67%), Gaps = 16/600 (2%) Query: 1 MGTFLYQKGFPVGISYEELNLTSPEVIEDVHRSYIGAGAVLLESNTYSANYDKLSKFGLE 60 MGT LY G V +EELNL+ PE I +H +YI AGA ++++NTY ANY KL+++GLE Sbjct: 18 MGTLLYSHG--VDRCFEELNLSKPEDIIHIHEAYIAAGAEVIQTNTYGANYVKLARYGLE 75 Query: 61 AKVGDINXXXXXXXXXXXXESGYVVGAVGSIRAGKRANLSGTELKRFYTEQFSALLEEQP 120 +V IN YV+G +G +R+ ++ +S E+KR + EQ LL E Sbjct: 76 DEVPSINRAAVRLAKKAAGNKAYVLGTIGGLRSINKSAVSIEEVKRTFREQLFVLLNEDV 135 Query: 121 DGILLETFYDVEELHLALRSVRKLSDLPVICQLAVDETARTLDGLTLPEAFHILEQDGAD 180 DG+LLET+YD+EEL L RK +D P+I +++ E DG L EA LEQ GAD Sbjct: 136 DGLLLETYYDLEELETVLAIARKETDKPIIAHVSLHEVGVLQDGTPLSEALARLEQLGAD 195 Query: 181 VIGFNCRTGPNGIKRALKSLQGNLKLPASIYPNAGIADYVDGQYRYGASPEYFGQMAPVF 240 V+G NCR GP + R+L+ + K S YPNA + DY DG+ Y + +YF + A F Sbjct: 196 VVGLNCRLGPYHMIRSLEEVPLPKKAFLSAYPNASLPDYRDGRLIYETNTDYFKETALAF 255 Query: 241 ADMGARIIGGCCGTTPQHIAEISAALRGYTPQPLPEPTGQRDPERLIVQEH--LAEDEER 298 + G R+IGGCCGTTP+HI I+ AL TP E+++ Q H ++ Sbjct: 256 REQGVRLIGGCCGTTPKHIEAIANALTDRTPIT----------EKVVKQRHVPISIQTNE 305 Query: 299 DGREPTLVDLVKERHTVIVELDPPRDLDIAKFMRGAEALRKAGADALTLADNSLAVTRMS 358 P L D+V++R +VIVELDPP+ L IAKF+ GA+AL++A DALTLADNSLA R+S Sbjct: 306 PNAAPPLQDIVRKRRSVIVELDPPKKLGIAKFLEGAKALKEANIDALTLADNSLATPRIS 365 Query: 359 NMALGHLVQARTGLRPLVHIACRDRNLIGTQSHLMGFDALGIDHVLAVTGDPARFGDLPG 418 NMALG +V+ + G+RPL+HI CRDRNLIG QSHLMG LGI VLA+TGDP++ GD PG Sbjct: 366 NMALGSIVKEQLGIRPLIHITCRDRNLIGLQSHLMGLHTLGITDVLAITGDPSKIGDFPG 425 Query: 419 SSSIYDLTSFEIIRMIKQLNDGIAFSGKPLKQNANFVIGAAFNPNVKHLDKAVERLEKKI 478 ++S+YDL+SF++IR+I+Q N+G+++SGK L Q NF I AAFNPNV+HLDKAVERLEKKI Sbjct: 426 ATSVYDLSSFDLIRLIRQFNEGLSYSGKSLGQKTNFSIAAAFNPNVRHLDKAVERLEKKI 485 Query: 479 ASGADYIMTQPVYDPELIARIAKATEHLEIPIFIGIMPLASGRNAEYLHNEVPGIQLSPE 538 GA Y +TQP+Y + I + +AT+HLE PI+IGIMPL S RNA++LH+EVPGI LS E Sbjct: 486 QCGAHYFITQPLYSEQQIEEVYEATKHLETPIYIGIMPLVSARNADFLHHEVPGITLSDE 545 Query: 539 VRSRMQGLEGEAGRA--EGVRIAKELLDAATAHFNGIYLMTPFMFYDMSVQLLEYVWAKQ 596 +RSRM ++ EG+ IAK L+DAA FNGIYL+TPF+ Y+M+V+L+ Y+ K+ Sbjct: 546 IRSRMAACANNPVQSAREGIAIAKSLIDAAFDLFNGIYLITPFLRYEMTVELVRYIHEKE 605 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,660,263,392 Number of extensions: 163018054 Number of successful extensions: 448577 Number of sequences better than 1.0e-05: 1725 Number of HSP's gapped: 444729 Number of HSP's successfully gapped: 1727 Length of query: 605 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 463 Effective length of database: 1,722,912,073 Effective search space: 797708289799 Effective search space used: 797708289799 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)