BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0040.p (347 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ34.1 B3KJ34_9BACI Radical SAM enzyme, Cfr family 639 0.0 B1DHL2.1 B1DHL2_9BACL Radical SAM enzyme, Cfr family 603 e-171 C3HMM5.1 C3HMM5_BACTU Radical SAM family enzyme 501 e-140 C3GMW7.1 C3GMW7_BACTU Radical SAM family enzyme 501 e-140 C3G6Y5.1 C3G6Y5_BACTU Radical SAM family enzyme 501 e-140 >B3KJ34.1 B3KJ34_9BACI Radical SAM enzyme, Cfr family Length = 346 Score = 639 bits (1648), Expect = 0.0, Method: Compositional matrix adjust. Identities = 299/344 (86%), Positives = 322/344 (93%) Query: 1 MKPLIYDFSLEELQQWAKDNGEPAFRGGQIFDWLYVKRVNDFEAMSNLSKALRAKLDEQF 60 MKP IYD+SLEELQ WA++NGEPAFRG QI+DWLYVKRVNDF M+NLSK LRAKL+++F Sbjct: 1 MKPFIYDYSLEELQAWAQENGEPAFRGTQIYDWLYVKRVNDFAEMTNLSKELRAKLEQEF 60 Query: 61 RIAALTEITKLESKDGTVKFLFGLHDDHAIETVIMKHNYGNSVCVTTQVGCRIGCTFCAS 120 LTEITKLESKDGTVKFLFGLHDDHAIETVIM+HNYGNS+CVTTQVGCRIGCTFCAS Sbjct: 61 SFVTLTEITKLESKDGTVKFLFGLHDDHAIETVIMRHNYGNSICVTTQVGCRIGCTFCAS 120 Query: 121 TLGGLKRDLTAGEIVAQVVRSQQILDARGERVSSIVIMGTGEPFENYDATMRFLRLMIHE 180 TLGGLKR+LT+GEIVAQVV++Q+ILD GERVSSIVIMG+GEPFENY+ATM+FLR MIHE Sbjct: 121 TLGGLKRNLTSGEIVAQVVQAQKILDKTGERVSSIVIMGSGEPFENYEATMKFLRTMIHE 180 Query: 181 KGLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRYPFDDV 240 KGLNIGQRHITVSTSGIVPNIYKF +EDTQINLAISIHAPND LRSKLMPVNRR+PFDDV Sbjct: 181 KGLNIGQRHITVSTSGIVPNIYKFTEEDTQINLAISIHAPNDKLRSKLMPVNRRFPFDDV 240 Query: 241 IESLRYYQAKTGRRISFEYALIGGVNDQKEHAEELAGVLKTMLCHVNLIPVNHVPERKYV 300 IESLRYYQAKTGRRI+FEYALIGGVNDQ EHAEELA V+K M C VNLIPVNHVPERKYV Sbjct: 241 IESLRYYQAKTGRRITFEYALIGGVNDQVEHAEELADVIKDMNCFVNLIPVNHVPERKYV 300 Query: 301 RTSRNDIFEFQRILADHGVNVTIRREQGHDIAAACGQLRAKHME 344 RTSRNDIF+FQR LAD GVNVTIRREQGHDIAAACGQLRAKHME Sbjct: 301 RTSRNDIFKFQRALADKGVNVTIRREQGHDIAAACGQLRAKHME 344 >B1DHL2.1 B1DHL2_9BACL Radical SAM enzyme, Cfr family Length = 348 Score = 603 bits (1556), Expect = e-171, Method: Compositional matrix adjust. Identities = 280/344 (81%), Positives = 312/344 (90%) Query: 1 MKPLIYDFSLEELQQWAKDNGEPAFRGGQIFDWLYVKRVNDFEAMSNLSKALRAKLDEQF 60 MKP+IYD++L++LQ W K+NGEPAFRGGQ+FDWLYVKRV FE MSNL K LR KL+E F Sbjct: 1 MKPIIYDYNLDQLQDWMKENGEPAFRGGQLFDWLYVKRVKSFEEMSNLPKPLREKLEESF 60 Query: 61 RIAALTEITKLESKDGTVKFLFGLHDDHAIETVIMKHNYGNSVCVTTQVGCRIGCTFCAS 120 + L+EITK ESKDGTVKFLFGLHD+HAIETVIM+H YGNS+CVTTQVGCRIGCTFCAS Sbjct: 61 QFVTLSEITKFESKDGTVKFLFGLHDNHAIETVIMRHEYGNSICVTTQVGCRIGCTFCAS 120 Query: 121 TLGGLKRDLTAGEIVAQVVRSQQILDARGERVSSIVIMGTGEPFENYDATMRFLRLMIHE 180 TLGGLKR+LTAGEIVAQVV +QQ+LDA GERVSSIVIMG+GEPFENYDATM FLR+MIHE Sbjct: 121 TLGGLKRNLTAGEIVAQVVTAQQMLDATGERVSSIVIMGSGEPFENYDATMTFLRIMIHE 180 Query: 181 KGLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRYPFDDV 240 KGLNIGQRHITVSTSGIVP++YKF +E+TQINLAISIHAPND LRSKLMPVNRR+PF+DV Sbjct: 181 KGLNIGQRHITVSTSGIVPSMYKFTEENTQINLAISIHAPNDKLRSKLMPVNRRFPFEDV 240 Query: 241 IESLRYYQAKTGRRISFEYALIGGVNDQKEHAEELAGVLKTMLCHVNLIPVNHVPERKYV 300 + + R + AKTGRRI+FEYALIGGVNDQ EHA+ELA VL+ MLCHVNLIPVNHVPER YV Sbjct: 241 MAACRNHIAKTGRRITFEYALIGGVNDQAEHAQELADVLQGMLCHVNLIPVNHVPERNYV 300 Query: 301 RTSRNDIFEFQRILADHGVNVTIRREQGHDIAAACGQLRAKHME 344 RT RNDIFEFQRIL + +N TIRREQGHDIAAACGQLRAKHME Sbjct: 301 RTPRNDIFEFQRILEKNKINCTIRREQGHDIAAACGQLRAKHME 344 >C3HMM5.1 C3HMM5_BACTU Radical SAM family enzyme Length = 362 Score = 501 bits (1289), Expect = e-140, Method: Compositional matrix adjust. Identities = 233/340 (68%), Positives = 270/340 (79%) Query: 2 KPLIYDFSLEELQQWAKDNGEPAFRGGQIFDWLYVKRVNDFEAMSNLSKALRAKLDEQFR 61 KP IY L E+Q W K+ GEP FR GQIFDWLY KRV ++E MSNLSK LR KL F Sbjct: 16 KPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFD 75 Query: 62 IAALTEITKLESKDGTVKFLFGLHDDHAIETVIMKHNYGNSVCVTTQVGCRIGCTFCAST 121 I L + K S DGT+KFLF L+D ++IETV+M+H YGNS+CVTTQVGCRIGCTFCAST Sbjct: 76 ITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCAST 135 Query: 122 LGGLKRDLTAGEIVAQVVRSQQILDARGERVSSIVIMGTGEPFENYDATMRFLRLMIHEK 181 LGGLKR+L AGEIVAQVV Q+ LD ERVSS+V+MG GEPF+NYD M FLR++ HEK Sbjct: 136 LGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEK 195 Query: 182 GLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRYPFDDVI 241 GL+IG RH+TVSTSGI+P IYKFA+ED QIN AIS+HAPN LRSKLMP+NR Y D++ Sbjct: 196 GLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRSKLMPINRAYKLPDLM 255 Query: 242 ESLRYYQAKTGRRISFEYALIGGVNDQKEHAEELAGVLKTMLCHVNLIPVNHVPERKYVR 301 E+++YY +TGRRI+FEY L GG NDQ EHAEELA +LK + CHVNLIPVN+VPER YVR Sbjct: 256 EAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVR 315 Query: 302 TSRNDIFEFQRILADHGVNVTIRREQGHDIAAACGQLRAK 341 T R IF F++ L D GVNVTIRREQGHDI AACGQLRAK Sbjct: 316 TPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAK 355 >C3GMW7.1 C3GMW7_BACTU Radical SAM family enzyme Length = 362 Score = 501 bits (1289), Expect = e-140, Method: Compositional matrix adjust. Identities = 233/340 (68%), Positives = 270/340 (79%) Query: 2 KPLIYDFSLEELQQWAKDNGEPAFRGGQIFDWLYVKRVNDFEAMSNLSKALRAKLDEQFR 61 KP IY L E+Q W K+ GEP FR GQIFDWLY KRV ++E MSNLSK LR KL F Sbjct: 16 KPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFD 75 Query: 62 IAALTEITKLESKDGTVKFLFGLHDDHAIETVIMKHNYGNSVCVTTQVGCRIGCTFCAST 121 I L + K S DGT+KFLF L+D ++IETV+M+H YGNS+CVTTQVGCRIGCTFCAST Sbjct: 76 ITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCAST 135 Query: 122 LGGLKRDLTAGEIVAQVVRSQQILDARGERVSSIVIMGTGEPFENYDATMRFLRLMIHEK 181 LGGLKR+L AGEIVAQVV Q+ LD ERVSS+V+MG GEPF+NYD M FLR++ HEK Sbjct: 136 LGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEK 195 Query: 182 GLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRYPFDDVI 241 GL+IG RH+TVSTSGI+P IYKFA+ED QIN AIS+HAPN LRSKLMP+NR Y D++ Sbjct: 196 GLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRSKLMPINRAYKLPDLM 255 Query: 242 ESLRYYQAKTGRRISFEYALIGGVNDQKEHAEELAGVLKTMLCHVNLIPVNHVPERKYVR 301 E+++YY +TGRRI+FEY L GG NDQ EHAEELA +LK + CHVNLIPVN+VPER YVR Sbjct: 256 EAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVR 315 Query: 302 TSRNDIFEFQRILADHGVNVTIRREQGHDIAAACGQLRAK 341 T R IF F++ L D GVNVTIRREQGHDI AACGQLRAK Sbjct: 316 TPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAK 355 >C3G6Y5.1 C3G6Y5_BACTU Radical SAM family enzyme Length = 362 Score = 501 bits (1289), Expect = e-140, Method: Compositional matrix adjust. Identities = 233/340 (68%), Positives = 270/340 (79%) Query: 2 KPLIYDFSLEELQQWAKDNGEPAFRGGQIFDWLYVKRVNDFEAMSNLSKALRAKLDEQFR 61 KP IY L E+Q W K+ GEP FR GQIFDWLY KRV ++E MSNLSK LR KL F Sbjct: 16 KPSIYSLQLHEMQDWLKEQGEPKFRAGQIFDWLYKKRVKNYEDMSNLSKGLREKLSNSFD 75 Query: 62 IAALTEITKLESKDGTVKFLFGLHDDHAIETVIMKHNYGNSVCVTTQVGCRIGCTFCAST 121 I L + K S DGT+KFLF L+D ++IETV+M+H YGNS+CVTTQVGCRIGCTFCAST Sbjct: 76 ITTLNTLVKQTSSDGTIKFLFQLYDGYSIETVLMRHEYGNSICVTTQVGCRIGCTFCAST 135 Query: 122 LGGLKRDLTAGEIVAQVVRSQQILDARGERVSSIVIMGTGEPFENYDATMRFLRLMIHEK 181 LGGLKR+L AGEIVAQVV Q+ LD ERVSS+V+MG GEPF+NYD M FLR++ HEK Sbjct: 136 LGGLKRNLEAGEIVAQVVEVQRALDESEERVSSLVVMGIGEPFDNYDNLMGFLRIINHEK 195 Query: 182 GLNIGQRHITVSTSGIVPNIYKFADEDTQINLAISIHAPNDALRSKLMPVNRRYPFDDVI 241 GL+IG RH+TVSTSGI+P IYKFA+ED QIN AIS+HAPN LRSKLMP+NR Y D++ Sbjct: 196 GLHIGARHMTVSTSGIIPKIYKFAEEDLQINFAISLHAPNSELRSKLMPINRAYKLPDLM 255 Query: 242 ESLRYYQAKTGRRISFEYALIGGVNDQKEHAEELAGVLKTMLCHVNLIPVNHVPERKYVR 301 E+++YY +TGRRI+FEY L GG NDQ EHAEELA +LK + CHVNLIPVN+VPER YVR Sbjct: 256 EAIKYYVNRTGRRITFEYGLFGGENDQVEHAEELAALLKGVKCHVNLIPVNYVPERDYVR 315 Query: 302 TSRNDIFEFQRILADHGVNVTIRREQGHDIAAACGQLRAK 341 T R IF F++ L D GVNVTIRREQGHDI AACGQLRAK Sbjct: 316 TPREQIFLFEKTLKDRGVNVTIRREQGHDIDAACGQLRAK 355 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,972,132,412 Number of extensions: 78275550 Number of successful extensions: 191237 Number of sequences better than 1.0e-05: 1528 Number of HSP's gapped: 186997 Number of HSP's successfully gapped: 1542 Length of query: 347 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 211 Effective length of database: 1,779,438,163 Effective search space: 375461452393 Effective search space used: 375461452393 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)