BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0037.p (297 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ37.1 B3KJ37_9BACI Ribosome small subunit-dependent GTPase A 438 e-121 B1DHK8.1 B1DHK8_9BACL Ribosome small subunit-dependent GTPase A 345 3e-93 B7GGG4.1 B7GGG4_ANOFW Predicted GTPase 273 2e-71 B4BJ88.1 B4BJ88_9BACI Ribosome small subunit-dependent GTPase A 270 2e-70 A4IM52.1 RSGA_GEOTN Putative ribosome biogenesis GTPase rsgA EC=... 270 2e-70 >B3KJ37.1 B3KJ37_9BACI Ribosome small subunit-dependent GTPase A Length = 302 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 211/278 (75%), Positives = 242/278 (87%), Gaps = 1/278 (0%) Query: 1 MPEGIIIKALSGYYYVKPLRDG-MIAAEEEAVQCRGRGILKKKGTAPLVGDRIKYVLTEN 59 M EG+I+KALSGYYYVKPL+DG +I+ E+ AVQCR RGI KKKG +PLVGDR+KY LTEN Sbjct: 1 MLEGLIVKALSGYYYVKPLQDGNIISGEDPAVQCRARGIFKKKGISPLVGDRVKYSLTEN 60 Query: 60 GEGMVDELLPRESELIRPPVANVKLAVLLFSVREPDMNLNLLDKFLVHIEHSGLETLIVL 119 GEG VDE+LPR S+LIRPPVANV LAVLLFSV+EPD++L+LLDKFLVHIEHSGLE LI L Sbjct: 61 GEGTVDEILPRSSQLIRPPVANVDLAVLLFSVKEPDLSLHLLDKFLVHIEHSGLEALICL 120 Query: 120 TKQDLAEDDGDATNQVKALYEHIGYEVIVTSSLTGSGSEELRERLAGIISVFSGQSGVGK 179 TKQDLA+D D QVK YE IGYEV++TSSL G G+EE+++RLA ISVFSGQSGVGK Sbjct: 121 TKQDLADDSDDVLEQVKRQYEEIGYEVLITSSLQGIGTEEVKDRLAARISVFSGQSGVGK 180 Query: 180 STLLNRLVPGLGLETGEISLRLGRGRHTTRHVELMDIGGGGFVADTPGFSQLDFLELGVE 239 S+LLN L+PGL LET EISLRLGRGRHTTRHVEL+++ GG+VADTPGFSQLDFLELGVE Sbjct: 181 SSLLNALMPGLSLETSEISLRLGRGRHTTRHVELIELDNGGYVADTPGFSQLDFLELGVE 240 Query: 240 ELSTCFREFAPYAENCKFRGCSHIHEPGCRVVEAVNSG 277 ELSTCF+EFAPYA CKFRGCSH+ EPGCRV++A G Sbjct: 241 ELSTCFKEFAPYAAECKFRGCSHMQEPGCRVIQAKEDG 278 >B1DHK8.1 B1DHK8_9BACL Ribosome small subunit-dependent GTPase A Length = 308 Score = 345 bits (885), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 178/282 (63%), Positives = 213/282 (75%), Gaps = 10/282 (3%) Query: 3 EGIIIKALSGYYYVKPLRDGMIAAEEEAVQCRGRGILKKKGTAPLVGDRIKYVLTENGEG 62 +G I+KALSGYYYV P D + + VQCR RG+ KK+G PLVGD + Y LTENGEG Sbjct: 8 KGRIVKALSGYYYVLP-HDA--ESPGQTVQCRARGVFKKRGLTPLVGDDVVYSLTENGEG 64 Query: 63 MVDELLPRESELIRPPVANVKLAVLLFSVREPDMNLNLLDKFLVHIEHSGLETLIVLTKQ 122 V+ELLPR SELIRPPVANV LA+L+FSV EP +NL LLDKFLVHIEHSG+E ++ L+KQ Sbjct: 65 TVEELLPRSSELIRPPVANVDLAILVFSVTEPTLNLQLLDKFLVHIEHSGIEAVLCLSKQ 124 Query: 123 DLAED------DGDATNQVKALYEHIGYEVIVTSSLTGSGSEELRERLAGIISVFSGQSG 176 DL ++ A V +Y IGYEV TSS G G EEL +RL G ++VF+GQSG Sbjct: 125 DLEQNGEETEEAIAAAEAVNRIYTAIGYEVYGTSSRQGVGVEELHDRLKGHVAVFAGQSG 184 Query: 177 VGKSTLLNRLVPGLGLETGEISLRLGRGRHTTRHVELMDIGGGGFVADTPGFSQLDFLEL 236 VGKS+LLN LVPGL LET EIS RLGRG+HTTRHVEL++I GGG+VADTPGFSQLDF EL Sbjct: 185 VGKSSLLNALVPGLELETNEISNRLGRGKHTTRHVELVEI-GGGYVADTPGFSQLDFTEL 243 Query: 237 GVEELSTCFREFAPYAENCKFRGCSHIHEPGCRVVEAVNSGA 278 G+E+L CFRE A +CKFR C+H+HEPGC V++AV G+ Sbjct: 244 GIEDLGYCFREMRELAPDCKFRACTHVHEPGCAVLKAVEEGS 285 >B7GGG4.1 B7GGG4_ANOFW Predicted GTPase Length = 329 Score = 273 bits (698), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 142/278 (51%), Positives = 188/278 (67%), Gaps = 10/278 (3%) Query: 1 MPEGIIIKALSGYYYVKPLRDGMIAAEEEAVQCRGRGILKKKGTAPLVGDRIKYVLTENG 60 M EG IIKALSG+YYV L DG + QCRGRG+ +K+ PLVGD + + + Sbjct: 37 MAEGKIIKALSGFYYV--LSDGQV------YQCRGRGVFRKRKVTPLVGDYVSFQVENER 88 Query: 61 EGMVDELLPRESELIRPPVANVKLAVLLFSVREPDMNLNLLDKFLVHIEHSGLETLIVLT 120 EG + ++ R++EL+RPP+AN+ A+L+FS EPD + LLD+FLV IE + +IV++ Sbjct: 89 EGYILDVFERKNELVRPPIANIDQAILVFSAVEPDFSPGLLDRFLVLIEAKNIRPMIVVS 148 Query: 121 KQDLAEDDGDA-TNQVKALYEHIGYEVIVTSSLTGSGSEELRERLAGIISVFSGQSGVGK 179 K DL +D+ Q Y IGY+V+ S T +G + L L G +SVF+GQSGVGK Sbjct: 149 KMDLVDDETKPRIEQYIRDYRQIGYDVLEVSVKTRAGVDALLPYLEGKVSVFAGQSGVGK 208 Query: 180 STLLNRLVPGLGLETGEISLRLGRGRHTTRHVELMDIGGGGFVADTPGFSQLDFLELGVE 239 S+LLN L PGL L+TG+IS LGRG+HTTRHVE ++I GGGFVADTPGFS L+F E+ +E Sbjct: 209 SSLLNALRPGLQLKTGDISHHLGRGKHTTRHVEFIEI-GGGFVADTPGFSALEFDEIELE 267 Query: 240 ELSTCFREFAPYAENCKFRGCSHIHEPGCRVVEAVNSG 277 +L CF EF + +CKFRGC+H EP C V EA+ SG Sbjct: 268 QLPLCFPEFVARSADCKFRGCTHTAEPKCAVKEALASG 305 >B4BJ88.1 B4BJ88_9BACI Ribosome small subunit-dependent GTPase A Length = 293 Score = 270 bits (689), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 144/278 (51%), Positives = 184/278 (66%), Gaps = 10/278 (3%) Query: 1 MPEGIIIKALSGYYYVKPLRDGMIAAEEEAVQCRGRGILKKKGTAPLVGDRIKYVLTENG 60 M EG IIKALSG+YYV +E + QCRGRG+ +K+ PLVGDR+ + T Sbjct: 1 MAEGQIIKALSGFYYV--------LSEGKVFQCRGRGVFRKQKVTPLVGDRVVFTATGEA 52 Query: 61 EGMVDELLPRESELIRPPVANVKLAVLLFSVREPDMNLNLLDKFLVHIEHSGLETLIVLT 120 EG + E+ R++EL+RPPVANV+ A+L+FS PD + LLD+FLV +E + +IV++ Sbjct: 53 EGYILEICERQNELVRPPVANVEQAILVFSAVSPDFSAKLLDRFLVLVESKNITPVIVIS 112 Query: 121 KQDLAEDDGDAT-NQVKALYEHIGYEVIVTSSLTGSGSEELRERLAGIISVFSGQSGVGK 179 K DL + T Q Y HIGYEVI TS++T G +EL L G +SV +GQSGVGK Sbjct: 113 KIDLLDGGMKETIAQYVRDYRHIGYEVIETSTVTKEGLDELMSHLCGRVSVVAGQSGVGK 172 Query: 180 STLLNRLVPGLGLETGEISLRLGRGRHTTRHVELMDIGGGGFVADTPGFSQLDFLELGVE 239 S+LLN L P L L+TG+IS LGRG+HTTRHVEL+ I GG VADTPGFS L+F + ++ Sbjct: 173 SSLLNALRPDLQLKTGDISTHLGRGKHTTRHVELLAI-AGGLVADTPGFSALEFDHIELD 231 Query: 240 ELSTCFREFAPYAENCKFRGCSHIHEPGCRVVEAVNSG 277 EL F EF Y E CKFRGC H+ EP C V EA +G Sbjct: 232 ELPYYFPEFRAYGEGCKFRGCLHVAEPKCAVREAAEAG 269 >A4IM52.1 RSGA_GEOTN Putative ribosome biogenesis GTPase rsgA EC=3.6.1.- Length = 293 Score = 270 bits (689), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 144/278 (51%), Positives = 184/278 (66%), Gaps = 10/278 (3%) Query: 1 MPEGIIIKALSGYYYVKPLRDGMIAAEEEAVQCRGRGILKKKGTAPLVGDRIKYVLTENG 60 M EG IIKALSG+YYV +E + QCRGRG+ +K+ PLVGDR+ + T Sbjct: 1 MAEGQIIKALSGFYYV--------LSEGKVFQCRGRGVFRKQKVTPLVGDRVVFTATGEA 52 Query: 61 EGMVDELLPRESELIRPPVANVKLAVLLFSVREPDMNLNLLDKFLVHIEHSGLETLIVLT 120 EG + E+ R++EL+RPPVANV+ A+L+FS PD + LLD+FLV +E + +IV++ Sbjct: 53 EGYILEICERQNELVRPPVANVEQAILVFSAVSPDFSAKLLDRFLVLVESKNITPVIVIS 112 Query: 121 KQDLAEDDGDAT-NQVKALYEHIGYEVIVTSSLTGSGSEELRERLAGIISVFSGQSGVGK 179 K DL + T Q Y HIGYEVI TS++T G +EL L G +SV +GQSGVGK Sbjct: 113 KIDLLDGGMKETIAQYVRDYRHIGYEVIETSTVTKEGLDELMSHLCGRVSVVAGQSGVGK 172 Query: 180 STLLNRLVPGLGLETGEISLRLGRGRHTTRHVELMDIGGGGFVADTPGFSQLDFLELGVE 239 S+LLN L P L L+TG+IS LGRG+HTTRHVEL+ I GG VADTPGFS L+F + ++ Sbjct: 173 SSLLNALRPDLQLKTGDISTHLGRGKHTTRHVELLAI-AGGLVADTPGFSALEFDHIELD 231 Query: 240 ELSTCFREFAPYAENCKFRGCSHIHEPGCRVVEAVNSG 277 EL F EF Y E CKFRGC H+ EP C V EA +G Sbjct: 232 ELPYYFPEFRAYGEGCKFRGCLHVAEPKCAVREAAEAG 269 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,749,750,266 Number of extensions: 75548754 Number of successful extensions: 317101 Number of sequences better than 1.0e-05: 1472 Number of HSP's gapped: 313749 Number of HSP's successfully gapped: 1477 Length of query: 297 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 162 Effective length of database: 1,788,859,178 Effective search space: 289795186836 Effective search space used: 289795186836 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)