BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0030.p (53 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFH4.1 B3KFH4_9BACI Regulatory protein TetR 99 1e-19 >B3KFH4.1 B3KFH4_9BACI Regulatory protein TetR Length = 219 Score = 99.4 bits (246), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 46/53 (86%), Positives = 51/53 (96%) Query: 1 MQFNLVMFFREFDIKDTKLQHKISKYFADFMGHGLLEDHKYSEIVSDFKKAKE 53 MQFNLVMFFREFDIKD+KLQHKISKYFADFM HGLLEDHKY+E+++D KKAKE Sbjct: 167 MQFNLVMFFREFDIKDSKLQHKISKYFADFMSHGLLEDHKYAEMLNDLKKAKE 219 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 292,796,809 Number of extensions: 7674076 Number of successful extensions: 24579 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 24579 Number of HSP's successfully gapped: 1 Length of query: 53 Length of database: 3,060,696,203 Length adjustment: 26 Effective length of query: 27 Effective length of database: 2,815,749,813 Effective search space: 76025244951 Effective search space used: 76025244951 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 125 (52.8 bits)