BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0022.p (232 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6N7.1 B3K6N7_9BACI NLP/P60 protein 131 6e-29 C4FPM0.1 C4FPM0_9FIRM Putative uncharacterized protein 114 6e-24 C0V413.1 C0V413_9FIRM Cell wall-associated hydrolase, invasion-a... 113 2e-23 C0ZCP7.1 C0ZCP7_BREBN Putative gamma-DL-glutamyl hydrolase EC=3.... 110 1e-22 B8I8D4.1 B8I8D4_CLOCE NLP/P60 protein 108 4e-22 >B3K6N7.1 B3K6N7_9BACI NLP/P60 protein Length = 164 Score = 131 bits (329), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 61/135 (45%), Positives = 87/135 (64%), Gaps = 3/135 (2%) Query: 98 IQSLSAADSSELVAYAKKYLGVPYEFGAGPYEETHRFDCSSFTRHVFKKFGVDLPRLAKD 157 +++ S + + +V + K+Y+G PY+FGA T FDCSSF +H+FKK+GV+LPR + Sbjct: 33 VEAASVSTGTRVVNFGKQYMGTPYKFGAST-STTRVFDCSSFMKHIFKKYGVNLPRTSAA 91 Query: 158 QDNLGRRVSRSELEIGDLIFFTVPGRFESDAIPGHVGIYIGNGKFIHTWGDPGVQISDLD 217 Q +G+ VS+S L GDL+FF+ R + HV +Y+GN K +HT+G PGV IS L Sbjct: 92 QSKVGKAVSKSNLRAGDLVFFSSGSRANGRNVT-HVAVYMGNNKILHTYGKPGVTISSL- 149 Query: 218 SGYWSNVILHMQRVL 232 SG WS L +RVL Sbjct: 150 SGNWSKTYLKARRVL 164 >C4FPM0.1 C4FPM0_9FIRM Putative uncharacterized protein Length = 269 Score = 114 bits (286), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 71/201 (35%), Positives = 110/201 (54%), Gaps = 28/201 (13%) Query: 38 LPDAPVRRNGQLYITPTALSKLLQTEVGWNPSTGEINIASPSDHKDSGHAGTTEQPKSLR 97 L AP R+ + +T A +K + T+ + S + + + SG + T K+++ Sbjct: 91 LMSAP--RSTKAVLTQNAATKAVATKSAFTNS-------NATSRRISGQSIT---GKNIK 138 Query: 98 IQSLSAADSSE----LVAYAKKYLGVPYEFGAGPYEETHRFDCSSFTRHVFKKFGVDLPR 153 + +++ +++ ++A A KY GVPY FG FDCS + ++VF K G+ LPR Sbjct: 139 LNNITKSETPNSIHAILAEADKYRGVPYVFGG---TTPSGFDCSGYVKYVFAKQGISLPR 195 Query: 154 LAKDQDNLGRRVSRSELEIGDLIFFTV--PGRFESDAIPGHVGIYIGNGKFIHTWGDPGV 211 LA +Q N+G VSR+ L+ GDL+FF PG P H GIYIG+GKFI GV Sbjct: 196 LADEQYNVGVEVSRANLKAGDLVFFETYEPG-------PSHSGIYIGDGKFISATSSRGV 248 Query: 212 QISDLDSGYWSNVILHMQRVL 232 ++DLD+GYW + +RV+ Sbjct: 249 AVADLDTGYWGERYIGAKRVI 269 >C0V413.1 C0V413_9FIRM Cell wall-associated hydrolase, invasion-associated protein Length = 270 Score = 113 bits (282), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 61/152 (40%), Positives = 90/152 (59%), Gaps = 16/152 (10%) Query: 87 AGTTEQPKSLRIQSLSAADSSE----LVAYAKKYLGVPYEFGAGPYEETHRFDCSSFTRH 142 AG + K++++ +++ ++ ++A A KY GVPY FG FDCS + ++ Sbjct: 128 AGQSINGKNVKLNNITKNETPSSIHAILAEADKYRGVPYVFGG---TTPSGFDCSGYVKY 184 Query: 143 VFKKFGVDLPRLAKDQDNLGRRVSRSELEIGDLIFFTV--PGRFESDAIPGHVGIYIGNG 200 VF+K G+ LPRLA +Q N+G VSR+ L+ GDL+FF PG P H GIYIG+G Sbjct: 185 VFEKQGISLPRLADEQYNVGVEVSRANLKAGDLVFFETYEPG-------PSHSGIYIGDG 237 Query: 201 KFIHTWGDPGVQISDLDSGYWSNVILHMQRVL 232 KFI GV ++DLD+GYW + +RV+ Sbjct: 238 KFISATSSRGVAVADLDTGYWGERYIGAKRVV 269 >C0ZCP7.1 C0ZCP7_BREBN Putative gamma-DL-glutamyl hydrolase EC=3.4.19.- Length = 293 Score = 110 bits (275), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 52/125 (41%), Positives = 76/125 (60%), Gaps = 2/125 (1%) Query: 108 ELVAYAKKYLGVPYEFGAGPYEETHRFDCSSFTRHVFKKFGVDLPRLAKDQDNLGRRVSR 167 +++ +KY+G Y++G+ T FDCSSFT+ VFK+ G+ LPR ++ Q +G+ VS+ Sbjct: 171 QVIKNGEKYMGTNYKYGSSS-STTKTFDCSSFTQRVFKEAGITLPRDSRQQSTVGKSVSK 229 Query: 168 SELEIGDLIFFTVPGRFESDAIPGHVGIYIGNGKFIHTWGDPGVQISDLDSGYWSNVILH 227 S L+ GDLIF SD I HV IY G+GK +HT+G PGV S D W + Sbjct: 230 SNLQKGDLIFMKASVNSTSDRIT-HVAIYAGDGKILHTYGSPGVTYSKYDGTNWEKRTVK 288 Query: 228 MQRVL 232 ++RV+ Sbjct: 289 IKRVI 293 >B8I8D4.1 B8I8D4_CLOCE NLP/P60 protein Length = 265 Score = 108 bits (270), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 58/150 (38%), Positives = 87/150 (58%), Gaps = 16/150 (10%) Query: 89 TTEQPKSLRIQSLSA----ADSSELVAYAKKYLGVPYEFGAGPYEETHRFDCSSFTRHVF 144 T +P + R + +A + +S +++ AKKY+GVPY +G FDCS + ++VF Sbjct: 126 TVTKPSTSRSNTSTAPATTSKASVVISTAKKYIGVPYVWGG---TTPSGFDCSGYIKYVF 182 Query: 145 KKFGVDLPRLAKDQDNLGRRVSRSELEIGDLIFFTV--PGRFESDAIPGHVGIYIGNGKF 202 K G+ LPR A +Q N G VS++ L+ GDL+FFT PG P H+GIY+GNG F Sbjct: 183 AKHGISLPRTAAEQYNAGSYVSKANLKAGDLVFFTTYKPG-------PSHLGIYLGNGSF 235 Query: 203 IHTWGDPGVQISDLDSGYWSNVILHMQRVL 232 IH GV IS L + Y++ + +R++ Sbjct: 236 IHASSSQGVIISSLSNSYFAERYIGARRII 265 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,488,296,084 Number of extensions: 62375201 Number of successful extensions: 139415 Number of sequences better than 1.0e-05: 2553 Number of HSP's gapped: 136521 Number of HSP's successfully gapped: 2565 Length of query: 232 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 101 Effective length of database: 1,826,543,238 Effective search space: 184480867038 Effective search space used: 184480867038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)