BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0012.p (317 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DJR2.1 B1DJR2_9BACL Binding-protein-dependent transport system... 413 e-113 O31519.1 YESP_BACSU Probable ABC transporter permease protein yesP 402 e-110 Q65KZ1.1 Q65KZ1_BACLD YesP 402 e-110 B3KGV2.1 B3KGV2_9BACI Binding-protein-dependent transport system... 374 e-102 B3K420.1 B3K420_9BACI Binding-protein-dependent transport system... 335 2e-90 >B1DJR2.1 B1DJR2_9BACL Binding-protein-dependent transport systems inner membrane component Length = 297 Score = 413 bits (1062), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/292 (69%), Positives = 240/292 (82%) Query: 25 RKNLTGYAFISPFIIGFLCFTLVPMAISLYLSFTKYNLFAPPRWIGFDNYIKMFTGDPKY 84 +++LTGYAFISPF++GFL FT VP+ SLYLSFT Y+LF P W+G DNY + T D K+ Sbjct: 6 KRDLTGYAFISPFVVGFLVFTFVPILASLYLSFTDYDLFTTPSWVGIDNYHDLLTSDKKF 65 Query: 85 VHSLKVTLLYVFIGVPLRLGFALFVAMILNTRSRMVGAYRTVYYLPSIIGGSVAVSIMWR 144 SLKVT LYVFIGVPLRL FAL VAM+LNT SR VG YRT++YLPSIIGGSVAVSIMWR Sbjct: 66 WKSLKVTFLYVFIGVPLRLFFALMVAMLLNTASRAVGVYRTLFYLPSIIGGSVAVSIMWR 125 Query: 145 NIFSDTGIINGMLGFFGLGPVSWFGNPNAALFMLITLSVWQFGSSMLIFLAGLKNISGEM 204 N++ D GIING+L F GL V WF +P AAL+MLITLS WQFGSSMLIFLAGLKNI + Sbjct: 126 NLYGDKGIINGLLQFVGLDAVRWFADPMAALWMLITLSAWQFGSSMLIFLAGLKNIPVSL 185 Query: 205 YEAASVDGASFFRKFFKITLPLLSSVILFNMVMQTISAFMTFVPAYIISKGEGGPMDGTM 264 YEA+SVDGA+ F +FFKIT+P+L+ +ILFN +MQTI AFMTFVPAYIISKG GGP+DGT+ Sbjct: 186 YEASSVDGANGFNRFFKITVPMLTPIILFNAIMQTIGAFMTFVPAYIISKGTGGPLDGTL 245 Query: 265 LYSLYLFRQAFMFNNMGYASAMAWVMLLLVGIMTGILFKTSKSWVFYESGGG 316 LYSLYLFRQ F F +MG ASAMAW+ML++V ++T +LF +SK WV YES G Sbjct: 246 LYSLYLFRQGFQFFDMGTASAMAWIMLVIVSVLTAVLFLSSKYWVHYESKEG 297 >O31519.1 YESP_BACSU Probable ABC transporter permease protein yesP Length = 309 Score = 402 bits (1034), Expect = e-110, Method: Compositional matrix adjust. Identities = 202/298 (67%), Positives = 241/298 (80%), Gaps = 2/298 (0%) Query: 17 KKPLGQRLRKNLTGYAFISPFIIGFLCFTLVPMAISLYLSFTKYNLFAPPRWIGFDNYIK 76 KK R + NL GYAFISPFIIGFLCFT++PM SL+LSFT Y+LF P+WIG DN+ + Sbjct: 10 KKSRSIR-KDNLAGYAFISPFIIGFLCFTVIPMGASLFLSFTSYDLFTAPKWIGLDNFKE 68 Query: 77 MFTGDPKYVHSLKVTLLYVFIGVPLRLGFALFVAMILNTRSRMVGAYRTVYYLPSIIGGS 136 MFTGD KY SLKVT YV GVPLRLGFALF+A+ILN ++ YRT++YLPSIIGGS Sbjct: 69 MFTGDEKYWQSLKVTFTYVLAGVPLRLGFALFIAVILNNAAKGTAIYRTLFYLPSIIGGS 128 Query: 137 VAVSIMWRNIFSDTGIINGMLGFFGLGP-VSWFGNPNAALFMLITLSVWQFGSSMLIFLA 195 VAV+IMWRNIF + G+IN +L F G+ + W+ NP +AL+ LI LSVWQFGSSMLIFLA Sbjct: 129 VAVAIMWRNIFGNDGVINALLFFVGIDQKILWYQNPTSALWTLILLSVWQFGSSMLIFLA 188 Query: 196 GLKNISGEMYEAASVDGASFFRKFFKITLPLLSSVILFNMVMQTISAFMTFVPAYIISKG 255 GLKNI EAASVDGA+ ++FFKITLP+L+ +I FN+VMQTISAFMTF PAYIISKG Sbjct: 189 GLKNIPSSYLEAASVDGANRVQRFFKITLPILTPIIFFNLVMQTISAFMTFTPAYIISKG 248 Query: 256 EGGPMDGTMLYSLYLFRQAFMFNNMGYASAMAWVMLLLVGIMTGILFKTSKSWVFYES 313 EGGP+DGT+LYSLYLF++AF + MGYASAMAWVML++VG++T ILFKTS WV YES Sbjct: 249 EGGPLDGTLLYSLYLFQRAFNYFQMGYASAMAWVMLVIVGLITLILFKTSSYWVHYES 306 >Q65KZ1.1 Q65KZ1_BACLD YesP Length = 299 Score = 402 bits (1033), Expect = e-110, Method: Compositional matrix adjust. Identities = 203/296 (68%), Positives = 239/296 (80%), Gaps = 1/296 (0%) Query: 22 QRLRKNLTGYAFISPFIIGFLCFTLVPMAISLYLSFTKYNLFAPPRWIGFDNYIKMFTGD 81 ++ NL GYAFISPFIIGFLCFT++PM SL+LSFT Y+LF PP+W+GFDNY M T D Sbjct: 4 KKRIDNLAGYAFISPFIIGFLCFTVIPMGASLFLSFTSYDLFTPPKWVGFDNYKAMLTDD 63 Query: 82 PKYVHSLKVTLLYVFIGVPLRLGFALFVAMILNTRSRMVGAYRTVYYLPSIIGGSVAVSI 141 KY SLKVT YV GVPLRL FALF+A+ILN + VG YRT++YLPSIIGGSVAV+I Sbjct: 64 EKYWGSLKVTFYYVLAGVPLRLAFALFIAVILNRAEKGVGIYRTLFYLPSIIGGSVAVAI 123 Query: 142 MWRNIFSDTGIINGMLGFFGLGP-VSWFGNPNAALFMLITLSVWQFGSSMLIFLAGLKNI 200 MWRN+F + G+ING+L F G + W+ +P +AL+ LI LSVWQFGSSMLIFLAGLKNI Sbjct: 124 MWRNVFGNDGVINGLLFFLGFDKKIFWYQDPTSALWTLILLSVWQFGSSMLIFLAGLKNI 183 Query: 201 SGEMYEAASVDGASFFRKFFKITLPLLSSVILFNMVMQTISAFMTFVPAYIISKGEGGPM 260 EAASVDGA+ F++FF ITLPLLS +I FN+VMQTISAFMTF PAYIISKGEGGP+ Sbjct: 184 PPMYQEAASVDGANRFQRFFLITLPLLSPIIFFNLVMQTISAFMTFTPAYIISKGEGGPL 243 Query: 261 DGTMLYSLYLFRQAFMFNNMGYASAMAWVMLLLVGIMTGILFKTSKSWVFYESGGG 316 DGT+LYSLYLF++AF F MGYASAMAWVML+L+G++T ILFKTS WV YES G Sbjct: 244 DGTLLYSLYLFQRAFNFFQMGYASAMAWVMLILIGLVTFILFKTSSLWVHYESKEG 299 >B3KGV2.1 B3KGV2_9BACI Binding-protein-dependent transport systems inner membrane component Length = 299 Score = 374 bits (960), Expect = e-102, Method: Compositional matrix adjust. Identities = 183/297 (61%), Positives = 231/297 (77%), Gaps = 3/297 (1%) Query: 22 QRLRKN--LTGYAFISPFIIGFLCFTLVPMAISLYLSFTKYNLFAPPRWIGFDNYIKMFT 79 +RLR+N +TGY F +PF++GFL FTL P+ SLY SFT YNL PRW+G NY ++F Sbjct: 2 KRLRENEHITGYVFSAPFVLGFLIFTLYPILSSLYYSFTNYNLMEAPRWLGLGNYERLFL 61 Query: 80 GDPKYVHSLKVTLLYVFIGVPLRLGFALFVAMILNTRSRMVGAYRTVYYLPSIIGGSVAV 139 D K S +VT YVF VPLRL FALFVAMILNT ++ VG YR+ +YLPS+IGGSVAV Sbjct: 62 EDDKIGKSFQVTFTYVFASVPLRLIFALFVAMILNTATKAVGLYRSAFYLPSLIGGSVAV 121 Query: 140 SIMWRNIFSDTGIINGMLGFFGL-GPVSWFGNPNAALFMLITLSVWQFGSSMLIFLAGLK 198 +IMW+ IF D G++N L FG+ SW GNP+ AL+ LI LS+WQFGSSM+IFLAGLK Sbjct: 122 AIMWQQIFGDKGLVNSALSLFGIHSTTSWIGNPSTALWTLIALSIWQFGSSMIIFLAGLK 181 Query: 199 NISGEMYEAASVDGASFFRKFFKITLPLLSSVILFNMVMQTISAFMTFVPAYIISKGEGG 258 NI + YEAASVDGA+ +FFKITLP+LS +ILFN+ +QTISAF+TF PAYIIS+GEGG Sbjct: 182 NIPKDYYEAASVDGANAVHRFFKITLPMLSPIILFNLTLQTISAFLTFTPAYIISRGEGG 241 Query: 259 PMDGTMLYSLYLFRQAFMFNNMGYASAMAWVMLLLVGIMTGILFKTSKSWVFYESGG 315 P+D T+LYSLYL+R+AF MGYASA+AW+ML++VG++T ++F++S WV YES G Sbjct: 242 PLDQTLLYSLYLYRKAFSHFEMGYASALAWIMLIIVGVLTLLIFRSSTRWVHYESKG 298 >B3K420.1 B3K420_9BACI Binding-protein-dependent transport systems inner membrane component Length = 304 Score = 335 bits (860), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 164/298 (55%), Positives = 219/298 (73%), Gaps = 4/298 (1%) Query: 15 RAKKPL-GQRLRKNLTGYAFISPFIIGFLCFTLVPMAISLYLSFTKYNLFAPPRWIGFDN 73 + KPL GQ +N T Y F+ P++IGF C TL PM SLYLS TK+NL + P W G N Sbjct: 3 KLSKPLYGQ---QNKTAYLFLLPWLIGFFCLTLGPMIASLYLSMTKFNLLSSPTWTGLSN 59 Query: 74 YIKMFTGDPKYVHSLKVTLLYVFIGVPLRLGFALFVAMILNTRSRMVGAYRTVYYLPSII 133 Y+++FT D + SL +T YVF+ VPLRL FAL +AM LN R +G YRTVYY+PS++ Sbjct: 60 YVQIFTEDDTFQRSLWLTFYYVFLSVPLRLAFALLIAMALNKGIRGLGIYRTVYYIPSLL 119 Query: 134 GGSVAVSIMWRNIFSDTGIINGMLGFFGLGPVSWFGNPNAALFMLITLSVWQFGSSMLIF 193 GGSVA++I+WR IF +G++N LG+FG+ SW +P+ ++ +ITLSVWQFGS+M+IF Sbjct: 120 GGSVAIAIVWRQIFDGSGLVNQFLGWFGITGPSWIAHPDYVVYTIITLSVWQFGSAMVIF 179 Query: 194 LAGLKNISGEMYEAASVDGASFFRKFFKITLPLLSSVILFNMVMQTISAFMTFVPAYIIS 253 LAGLK I ++YEA+ VDGA R+FF ITLP+LS VI FN++M I++F F PAY+I Sbjct: 180 LAGLKQIPTDLYEASGVDGAGKIRQFFGITLPMLSPVIFFNLIMSMINSFQAFTPAYVIG 239 Query: 254 KGEGGPMDGTMLYSLYLFRQAFMFNNMGYASAMAWVMLLLVGIMTGILFKTSKSWVFY 311 G GGP+D TM Y+LYL+ + F F +MGYASA+AW+ML+++G+ TGI+F TSK WVFY Sbjct: 240 DGRGGPLDATMFYTLYLYLKGFSFFDMGYASALAWIMLVIIGVFTGIVFLTSKFWVFY 297 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,956,891,637 Number of extensions: 82087162 Number of successful extensions: 308895 Number of sequences better than 1.0e-05: 5998 Number of HSP's gapped: 296401 Number of HSP's successfully gapped: 6012 Length of query: 317 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 182 Effective length of database: 1,788,859,178 Effective search space: 325572370396 Effective search space used: 325572370396 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)