BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0010.p (263 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIU1.1 B3KIU1_9BACI Short-chain dehydrogenase/reductase SDR 312 2e-83 B1DH61.1 B1DH61_9BACL Short-chain dehydrogenase/reductase SDR 296 1e-78 C1P8U9.1 C1P8U9_BACCO Short-chain dehydrogenase/reductase SDR 295 4e-78 Q2B2W0.1 Q2B2W0_9BACI Putative uncharacterized protein 290 7e-77 C0UWD7.1 C0UWD7_9BACT Putative uncharacterized protein 287 6e-76 >B3KIU1.1 B3KIU1_9BACI Short-chain dehydrogenase/reductase SDR Length = 263 Score = 312 bits (799), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 149/237 (62%), Positives = 179/237 (75%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 L A GAK+ ++D E E V+K CDI++ + +++ QAA QWG+ Sbjct: 27 LLMAEQGAKLLLIDIDRERVENVKKTIEDGGGEVLTGNCDISKAESLKDCFSQAAEQWGK 86 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYLKANGGSILINSSI 145 +DIVFANAGING PIE M+++ WD T+N NLRGTFATVKYAIPYLK +GGSI+I SSI Sbjct: 87 IDIVFANAGINGTKAPIEIMELDEWDATINTNLRGTFATVKYAIPYLKDHGGSIIITSSI 146 Query: 146 NGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSED 205 NGNRVF+ GFSAYSTTKAGQ AFMKMAALELA+YKIRVNAICPGAI TNID++T + Sbjct: 147 NGNRVFTGFGFSAYSTTKAGQVAFMKMAALELARYKIRVNAICPGAIATNIDESTIAKPE 206 Query: 206 LKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGAESLLH 262 L EV I +E+P+G QPL GPG+P QVA+L FLASDD+ HITGTEIY DGAESL+H Sbjct: 207 LDEVTIPIEYPEGDQPLADGPGQPSQVAQLVAFLASDDASHITGTEIYIDGAESLIH 263 >B1DH61.1 B1DH61_9BACL Short-chain dehydrogenase/reductase SDR Length = 267 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 140/238 (58%), Positives = 176/238 (73%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 + FA GA + +LDR + V ++ +E DI++ + A +A +G+ Sbjct: 30 IRFAEEGANLVLLDRDRQTLSVVCREAEAFGAKSIAVEADISDEHSLAEAYDKAISAFGQ 89 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYLKANGGSILINSSI 145 LDIVFANAGING ++PIE M E W+ T+N+NL GTF TVK+AIP LK GGSI+I SSI Sbjct: 90 LDIVFANAGINGTISPIEHMSKEEWEATINVNLTGTFLTVKHAIPLLKEKGGSIIITSSI 149 Query: 146 NGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSED 205 NGNRVFSN GFSAYS++KAG+TAF +MAALELA+Y IRVNAICPGAI+TNID +T P + Sbjct: 150 NGNRVFSNFGFSAYSSSKAGETAFTRMAALELAEYGIRVNAICPGAISTNIDQSTKPKPE 209 Query: 206 LKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGAESLLHG 263 L+E++I VEFP+GG PLE GPG+P QVA L LFLASD++ HITGT +Y DGAESLL G Sbjct: 210 LEEIRIPVEFPEGGHPLEDGPGQPAQVANLVLFLASDEASHITGTSVYIDGAESLLRG 267 >C1P8U9.1 C1P8U9_BACCO Short-chain dehydrogenase/reductase SDR Length = 260 Score = 295 bits (754), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 136/238 (57%), Positives = 180/238 (75%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 L A GAKV ++D + A++V+++ +E D+ +P+QV+ AV + +W R Sbjct: 23 LKLAAEGAKVCVMDIEQKRADEVKRRIEQNGGEAMALEVDVTDPEQVKTAVDKTVREWDR 82 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYLKANGGSILINSSI 145 LDIVF+NAGING + PIE + + W++T++ NL+GTF KYAIP++K GGSI+I SSI Sbjct: 83 LDIVFSNAGINGTVAPIEDLTPDDWEKTLSTNLKGTFLLTKYAIPHMKNRGGSIIITSSI 142 Query: 146 NGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSED 205 NGNR+FSNIG SAYS++KAGQ AFMKMAALELA+YKIRVNA+CPGAI T I+ NT +E+ Sbjct: 143 NGNRIFSNIGMSAYSSSKAGQVAFMKMAALELARYKIRVNAVCPGAIETKIEQNTNRTEE 202 Query: 206 LKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGAESLLHG 263 L++VQI +EFP+G QPL PG+P++VA L LFLASDDS HI+GT+IY DG ESLL G Sbjct: 203 LEKVQIPIEFPEGDQPLSGRPGKPEEVADLVLFLASDDSSHISGTDIYIDGTESLLRG 260 >Q2B2W0.1 Q2B2W0_9BACI Putative uncharacterized protein Length = 262 Score = 290 bits (743), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 141/237 (59%), Positives = 173/237 (72%), Gaps = 1/237 (0%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 + A+ GAK+A++D E A++V+ Q IE D++ P QVE A ++ + +WGR Sbjct: 26 IRLAKEGAKIALVDVKEEFAKEVKDQIESAGGEAMIIETDVSVPLQVEEAFQKVSSKWGR 85 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYLKANGGSILINSSI 145 LDIVFANAGING + PIE + E WD T++ NL+GTF TVKYA+P LK GGSI+I SSI Sbjct: 86 LDIVFANAGINGVVAPIEDLTPEEWDTTISTNLKGTFLTVKYAVPLLKEEGGSIIITSSI 145 Query: 146 NGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSED 205 NGNR F N G SAYST+K GQ +F KMAALELA Y+IRVN ICPGAI TNI +NT+P E+ Sbjct: 146 NGNRTFRNFGMSAYSTSKGGQVSFGKMAALELAGYRIRVNLICPGAIRTNIGENTFPEEE 205 Query: 206 -LKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGAESLL 261 LK+V+I VEFP+G PLE PG +QVA L FLASD S HITGTE+Y DGAESLL Sbjct: 206 NLKKVKIPVEFPEGSMPLEDHPGSAEQVADLVHFLASDLSSHITGTEVYIDGAESLL 262 >C0UWD7.1 C0UWD7_9BACT Putative uncharacterized protein Length = 262 Score = 287 bits (735), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 145/263 (55%), Positives = 176/263 (66%), Gaps = 1/263 (0%) Query: 1 MADKKLEXXXXXXXXXXXXXXXXXXLAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXX 60 M D +L+ + A +GAKV +LD + AEKV KQ Sbjct: 1 MVDLRLDGKVSLVTGGGSGIGEAAAVLMADNGAKVGVLDFRQDAAEKVSKQIVNRGGSAI 60 Query: 61 XIECDIAEPQQVENAVKQAAGQWGRLDIVFANAGINGAMTPIETMDIESWDQTMNINLRG 120 + D++ P+Q+ +A+ Q +WGRLDIVFANAGING P+E +D + WD+T+ INL+G Sbjct: 61 PLVADVSNPEQMNSAIGQLVNEWGRLDIVFANAGINGVWAPLEELDPQEWDRTLAINLKG 120 Query: 121 TFATVKYAIPYLKANGGSILINSSINGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQY 180 TF TVKYAIPYLK GGSI+I SSING RVFSN G +AYS +K Q F +MAALELAQY Sbjct: 121 TFLTVKYAIPYLKERGGSIIITSSINGTRVFSNTGATAYSCSKGAQVVFTQMAALELAQY 180 Query: 181 KIRVNAICPGAITTNIDDNTYPSEDLKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLA 240 KIRVNAICPGAI TNID+ T D +EV++ VEFP+G PLE GPG P QVA L LFLA Sbjct: 181 KIRVNAICPGAIETNIDERT-EKRDTEEVEVPVEFPEGSFPLEGGPGSPQQVANLVLFLA 239 Query: 241 SDDSDHITGTEIYCDGAESLLHG 263 SD S HITGT I+ +GAESLL G Sbjct: 240 SDASSHITGTPIWIEGAESLLRG 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,289,498,255 Number of extensions: 49344829 Number of successful extensions: 197430 Number of sequences better than 1.0e-05: 22950 Number of HSP's gapped: 165616 Number of HSP's successfully gapped: 23015 Length of query: 263 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 130 Effective length of database: 1,807,701,208 Effective search space: 235001157040 Effective search space used: 235001157040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)