BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0032.p (510 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase 472 e-131 B8I5I0.1 B8I5I0_CLOCE Condensation domain protein 460 e-127 B8I978.1 B8I978_CLOCE Beta-ketoacyl synthase 405 e-111 B9E3Q1.1 B9E3Q1_CLOK1 Putative uncharacterized protein 393 e-107 A5MZS1.1 A5MZS1_CLOK5 Predicted hybrid nonribosomal peptide synt... 393 e-107 >B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase Length = 1613 Score = 472 bits (1214), Expect = e-131, Method: Compositional matrix adjust. Identities = 243/533 (45%), Positives = 332/533 (62%), Gaps = 39/533 (7%) Query: 3 IGIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNSI 62 IGI SSD K ++FDDSS+GTG GEG A+LLKPLKKA+ DRD+++AVIKGSAINQDGNSI Sbjct: 254 IGITSSDGKARSFDDSSDGTGIGEGCAAILLKPLKKAIADRDNVYAVIKGSAINQDGNSI 313 Query: 63 GITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKK 122 GITAP+ AQEEV+ +AW++AG+NPE +SYIEAHGTGT+LGDP+EI+GI+ AF RYT KK Sbjct: 314 GITAPNSAAQEEVIVKAWKDAGVNPETISYIEAHGTGTKLGDPLEIDGIESAFKRYTDKK 373 Query: 123 QFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVND 182 QFCA+G++KTNIGHLD+A+G+AG++K++ +L +K+L P LHF +PN+ N S VY+ND Sbjct: 374 QFCALGTVKTNIGHLDNASGMAGVIKMVMALTNKELPPMLHFKRPNRKTNIQESPVYIND 433 Query: 183 RLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAPPPPQKQQHGETMYL------LPLSAK 236 RL +W T G PR GISSFGLSGTNCHM+LEEAP + Q E + L +SA Sbjct: 434 RLTQWETDGFPRRCGISSFGLSGTNCHMILEEAPSSEKPQYPAEESIIPYRKNVLTISAN 493 Query: 237 EENVLRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLERLT 296 + L+ AY +L+ + LED+CYTA+TGR H+ R+AI+ T +DL +L LT Sbjct: 494 HVSALQSYIEAYRHMLDNIKNVTLEDICYTANTGRTHFPVRIAIITTDIKDLKQKLAFLT 553 Query: 297 EK--------------------------QALTPTLQSAQQLKGMEHSEKENSYRAIAELY 330 + LTP + L I + Y Sbjct: 554 GSIKYNIDTKCIYSAKEYATDHPGISTFENLTPEADTLPDLTSASGDIYNKHLDEICKSY 613 Query: 331 VSGGTIDWEDMYKGKACNRLSLPVYPFQKTRCWL---KPEAQAEPKRSASGGNV-HPLLA 386 +SG I W+ +Y GK ++SLP YPF++ RCWL P+A S + +PLL Sbjct: 614 ISGDHIQWDKVYDGKGMKKVSLPTYPFERLRCWLTANTPKAAEINAYEQSYNKLGYPLLD 673 Query: 387 HVAGETPEQVTYAVRMSAKEDWMLNEHKVGSEYVLPGTAYVELLLE-IGRQQGGRSV-IK 444 + ET E Y+ W+LNEHKV +++PGT+Y+E++ E +G + ++ + Sbjct: 674 SCSTETMEGAVYSTNFQVNRHWVLNEHKVNGNFIVPGTSYIEMVSEAVGHYKYYENLEFR 733 Query: 445 DLAFLQPFSVQEGETRELQITLKKGNGDNGFIITSKDYADGTWRRHAEGKLCL 497 D+ F P +V E E R++Q LK +G GF + S+ D W RH +GK+ L Sbjct: 734 DIIFEFPLAVGENEARDVQTVLKIEDGYLGFYVASRPDGD-KWVRHVQGKVFL 785 >B8I5I0.1 B8I5I0_CLOCE Condensation domain protein Length = 2006 Score = 460 bits (1183), Expect = e-127, Method: Compositional matrix adjust. Identities = 241/549 (43%), Positives = 342/549 (62%), Gaps = 45/549 (8%) Query: 3 IGIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNSI 62 +GI SSD K +TFDD+S+GTG GEG AVLLKPL++AL D+DHI+AVIKGSA N DG+SI Sbjct: 244 VGIASSDGKARTFDDNSDGTGIGEGVAAVLLKPLEQALNDKDHIYAVIKGSAANNDGSSI 303 Query: 63 GITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKK 122 GITAP+ AQ + + +AWE++GINPE LSYIEAHGTGT+LGDPIEI+GI RAF +YT KK Sbjct: 304 GITAPNSLAQRDAIVKAWEDSGINPETLSYIEAHGTGTKLGDPIEIDGIKRAFKKYTDKK 363 Query: 123 QFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVND 182 QFCAIGS+K+NIGHLD+ AGIAGL+K + ++ ++ P+LHF +PN+ I F S VYVND Sbjct: 364 QFCAIGSVKSNIGHLDNVAGIAGLIKAVLCIQYGQIPPTLHFKRPNRKIMFEDSPVYVND 423 Query: 183 RLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAPPPPQKQQHGET-MYLLPLSAKEENVL 241 RL EW T G PR GISSFG+SGTNCHM+LEEAP K ++ ++ + +L LSAK E L Sbjct: 424 RLHEWETMGYPRRCGISSFGISGTNCHMILEEAPNSKGKSENPQSKLQVLTLSAKNEKSL 483 Query: 242 RELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLERL------ 295 L ++ L+ + ++ +ED+C+T +TGR H++ R+A++ D+ ++ +L Sbjct: 484 YSLLKSFDEYLKKNKEVSIEDICFTQNTGRMHHSLRIALILENRHDMEEKISKLADLQPK 543 Query: 296 -----------------------------TEKQALTPTLQSAQQLKGMEHSEKEN--SYR 324 T+K+ L+ T ++++LK + S N + Sbjct: 544 GIREQGVYYGYHKVVRNSEGIKNDGYISETDKRELSKT--ASERLKELVTSGNYNRGTLE 601 Query: 325 AIAELYVSGGTIDWEDMYKGKACNRLSLPVYPFQKTRCWLKPEAQAEPKRSASGGNVHPL 384 I LY +GG IDW+ +YK + N++SLP YPF + R W+ + Q + S G + L Sbjct: 602 EICILYTTGGDIDWDFLYKSE--NKVSLPTYPFNRKRFWIDTKRQMKESVQESHGERYKL 659 Query: 385 LAHVAGETPEQVTYAVRMSAKEDWMLNEHKVGSEYVLPGTAYVELLLEIGRQQG-GRSV- 442 + +T Q Y +S + W+L EHKVG Y+L GTAY+EL ++ ++ G++V Sbjct: 660 PGYRISDTINQEVYTAEISVENQWLLREHKVGGNYMLVGTAYLELAMQTSKKYYPGKAVE 719 Query: 443 IKDLAFLQPFSVQEGETRELQITLKKGNGDNGFIITSKD-YADGTWRRHAEGKLCLPSQA 501 I++L L P V E + EL + K G F+I SK A+ W HA+G++ + Sbjct: 720 IRELTLLTPLIVGEEDKVELHTVIMKEYGFLKFVIASKSKTAENGWIEHAQGRIYGTEKL 779 Query: 502 RKPYDLREG 510 YD+ E Sbjct: 780 SDKYDMTEA 788 >B8I978.1 B8I978_CLOCE Beta-ketoacyl synthase Length = 1272 Score = 405 bits (1041), Expect = e-111, Method: Compositional matrix adjust. Identities = 196/399 (49%), Positives = 268/399 (67%), Gaps = 32/399 (8%) Query: 1 LKIGIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGN 60 L GI +SD + KTFDDSS+GTG GEG LLKPL +AL+D+D+IHAVIKGSAINQDG Sbjct: 237 LNFGINASDGRAKTFDDSSDGTGSGEGVITYLLKPLSRALKDKDNIHAVIKGSAINQDGR 296 Query: 61 SIGITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQ 120 SIG++AP+ AQE+V+ AW++AGI+PE +SYIEAHGTGT+LGDPIE++GI RAF RYT Sbjct: 297 SIGLSAPNVLAQEDVIVSAWKDAGIDPETVSYIEAHGTGTKLGDPIEVDGISRAFRRYTS 356 Query: 121 KKQFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYV 180 KKQFC+IGS+KTNIGHLD+AAG+AG++K + SLK+K+L SLH+ PN+ INF S VYV Sbjct: 357 KKQFCSIGSVKTNIGHLDNAAGVAGMLKAVLSLKNKQLPASLHYKVPNRRINFKDSPVYV 416 Query: 181 NDRLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAPPPPQKQQHGETMYLLPLSAKEENV 240 ND+L W T G PR G+SSFG SGTNCH++LEEAP K + +L LSAK + Sbjct: 417 NDKLTPWETHGFPRRCGVSSFGFSGTNCHIILEEAPSVVNKSIDRPCLEVLTLSAKTNDA 476 Query: 241 LRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLERLT---- 296 L+EL Y ++ + +D+ ++CYT +TGR H+++R+A++ +L N+L++L Sbjct: 477 LKELVKRYRLYIQKNEDVDIGNMCYTQNTGRWHFDYRIAVIARNTIELKNKLDKLVSIDF 536 Query: 297 --------------------------EKQALTPTLQSAQQLKGMEHSEK--ENSYRAIAE 328 E + + ++ ++L+ SEK E+ I + Sbjct: 537 EDINEKEIYFGIQLDNIKQKGQNGLFESKIKELSCEADKKLEEFILSEKTEEDVLYDICK 596 Query: 329 LYVSGGTIDWEDMYKGKACNRLSLPVYPFQKTRCWLKPE 367 YV G IDW+ +YKG+ R+SLP YPF++TRCW+ E Sbjct: 597 RYVEGANIDWDILYKGEDRTRISLPTYPFRRTRCWINAE 635 >B9E3Q1.1 B9E3Q1_CLOK1 Putative uncharacterized protein Length = 1190 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 192/393 (48%), Positives = 256/393 (65%), Gaps = 34/393 (8%) Query: 2 KIGIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNS 61 KIGIESSD KT+ FD++S+GTG GEG AVLLKPL KAL+D D+I+A+IKGSA+NQDG S Sbjct: 242 KIGIESSDGKTRAFDENSDGTGIGEGVAAVLLKPLNKALKDGDNIYAIIKGSAVNQDGKS 301 Query: 62 IGITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQK 121 IGITAP+ +AQ V+ +AW+ AGINPE +SYIEAHGTGT+LGDPIEI+GI +AF RYT K Sbjct: 302 IGITAPNVKAQSSVIIKAWKNAGINPETISYIEAHGTGTKLGDPIEIDGIKKAFERYTHK 361 Query: 122 KQFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVN 181 KQFC IGS+K+NIGHL AGIA +KV+ S++ + + PS++F PN++INF S +YVN Sbjct: 362 KQFCGIGSVKSNIGHLYDCAGIASFIKVVLSIRHRYIPPSINFFTPNKSINFEDSPLYVN 421 Query: 182 DRLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAP------PPPQKQQHGETMYLLPLSA 235 DRL++W P AG+SSFG SGTNCH+VLEEAP P +H + +SA Sbjct: 422 DRLKKWNIRSGPLRAGVSSFGFSGTNCHIVLEEAPFENNLEKPEVNFEHN----IFVISA 477 Query: 236 KEENVLRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLERL 295 K E L+EL Y L+ + I+ +D+CYTASTGR+HYN R+A++ DL +L+ L Sbjct: 478 KTEKTLKELILKYNTFLKNAKDINFKDICYTASTGRKHYNFRIAVISKDTLDLREKLDYL 537 Query: 296 T-----------------------EKQALTPTLQSAQQLKGMEHSEKENSYRAIAELYVS 332 E++ + +L+ + HS+ + + I +YV Sbjct: 538 CCTDSYESVKYNSNIFYGCNILTGEREKINISLEDVMN-RFFHHSKSMDVLKEICSMYVK 596 Query: 333 GGTIDWEDMYKGKACNRLSLPVYPFQKTRCWLK 365 G W YK + ++SLP Y FQK RCWL+ Sbjct: 597 GADFKWSRFYKNERRKKISLPTYSFQKERCWLE 629 >A5MZS1.1 A5MZS1_CLOK5 Predicted hybrid nonribosomal peptide synthetase/polyketide synthase Length = 1190 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 192/393 (48%), Positives = 256/393 (65%), Gaps = 34/393 (8%) Query: 2 KIGIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNS 61 KIGIESSD KT+ FD++S+GTG GEG AVLLKPL KAL+D D+I+A+IKGSA+NQDG S Sbjct: 242 KIGIESSDGKTRAFDENSDGTGIGEGVAAVLLKPLNKALKDGDNIYAIIKGSAVNQDGKS 301 Query: 62 IGITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQK 121 IGITAP+ +AQ V+ +AW+ AGINPE +SYIEAHGTGT+LGDPIEI+GI +AF RYT K Sbjct: 302 IGITAPNVKAQSSVIIKAWKNAGINPETISYIEAHGTGTKLGDPIEIDGIKKAFERYTHK 361 Query: 122 KQFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVN 181 KQFC IGS+K+NIGHL AGIA +KV+ S++ + + PS++F PN++INF S +YVN Sbjct: 362 KQFCGIGSVKSNIGHLYDCAGIASFIKVVLSIRHRYIPPSINFFTPNKSINFEDSPLYVN 421 Query: 182 DRLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAP------PPPQKQQHGETMYLLPLSA 235 DRL++W P AG+SSFG SGTNCH+VLEEAP P +H + +SA Sbjct: 422 DRLKKWNIRSGPLRAGVSSFGFSGTNCHIVLEEAPFENNLEKPEVNFEHN----IFVISA 477 Query: 236 KEENVLRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLERL 295 K E L+EL Y L+ + I+ +D+CYTASTGR+HYN R+A++ DL +L+ L Sbjct: 478 KTEKTLKELILKYNTFLKNAKDINFKDICYTASTGRKHYNFRIAVISKDTLDLREKLDYL 537 Query: 296 T-----------------------EKQALTPTLQSAQQLKGMEHSEKENSYRAIAELYVS 332 E++ + +L+ + HS+ + + I +YV Sbjct: 538 CCTDSYESVKYNSNIFYGCNILTGEREKINISLEDVMN-RFFHHSKSMDVLKEICSMYVK 596 Query: 333 GGTIDWEDMYKGKACNRLSLPVYPFQKTRCWLK 365 G W YK + ++SLP Y FQK RCWL+ Sbjct: 597 GADFKWSRFYKNERRKKISLPTYSFQKERCWLE 629 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,278,614,862 Number of extensions: 142252893 Number of successful extensions: 281313 Number of sequences better than 1.0e-05: 8854 Number of HSP's gapped: 268534 Number of HSP's successfully gapped: 10949 Length of query: 510 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 370 Effective length of database: 1,741,754,103 Effective search space: 644449018110 Effective search space used: 644449018110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)