BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0016.p (240 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I7C8.1 B8I7C8_CLOCE Thioesterase 219 2e-55 C1FRI1.1 C1FRI1_CLOBJ Linear gramicidin dehydrogenase LgrE EC=1.... 212 2e-53 C3AE80.1 C3AE80_BACMY Thioesterase 209 1e-52 Q2XP41.1 Q2XP41_BACSU SubT 209 2e-52 C3BKA1.1 C3BKA1_9BACI Thioesterase 208 3e-52 >B8I7C8.1 B8I7C8_CLOCE Thioesterase Length = 235 Score = 219 bits (557), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 104/229 (45%), Positives = 155/229 (67%), Gaps = 1/229 (0%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 + LFC PYAGGS++VF+K KR D I+++PVELAGRG+R EPFY ++++ + DI I Sbjct: 5 INLFCFPYAGGSSSVFSKLKRYADKNIKVVPVELAGRGQRIGEPFYKNMNEAINDIYDKI 64 Query: 71 MDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPP-HIQDKKKIHHLP 129 ++ ++ FGHSMG+ + EL +I N ++P HLF+SG PP + +D KKIH LP Sbjct: 65 SSFTYEQPFAFFGHSMGSSMVFELSHKILQNQKKEPIHLFISGRCPPINHKDDKKIHGLP 124 Query: 130 DDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLVG 189 D++F +EV LGGTPK+V EN EL +F P+LR+DYK VE Y K DI+V G Sbjct: 125 DNKFIEEVYELGGTPKEVFENMELRRVFLPILRADYKLVEEYQYFEKANKLSCDISVFNG 184 Query: 190 DEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTL 238 +D +T++ ++ W +YT+ +I F GGHFF++ + K+++D++N+TL Sbjct: 185 KDDRMTNLDGMKGWGDYTSGKTNIFEFDGGHFFLHNQMKEIMDIMNKTL 233 >C1FRI1.1 C1FRI1_CLOBJ Linear gramicidin dehydrogenase LgrE EC=1.1.-.- Length = 238 Score = 212 bits (540), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 101/230 (43%), Positives = 155/230 (67%), Gaps = 2/230 (0%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 M LFCLPYAGGS ++ KWK+ L+ IEL P+EL GRG+R+ E FY ++ + V DI I Sbjct: 1 MILFCLPYAGGSEAIYYKWKKYLNPSIELSPIELKGRGKRYNEIFYETIDEAVEDILKNI 60 Query: 71 MDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPH-IQDKKKIHHLP 129 D+I D Y+++GHSMG+L EL +I+ KP H+F SG P+ I+ K+ I+ LP Sbjct: 61 KDRIIDNDYAIYGHSMGSLLAYELYYKIKKQKLRKPIHIFFSGYKAPNIIRKKENIYTLP 120 Query: 130 DDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLVG 189 D +F ++VI LGGTP+++S NKEL++IF P++R+D+K +E+Y E ++K + D+++L G Sbjct: 121 DYDFIKKVIELGGTPEELSNNKELLDIFIPIIRNDFKILENYNYEKKEDKIECDVSILNG 180 Query: 190 DEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTLI 239 +D I ++ +I W ++ + + +F+G HFFIN K + +IN TLI Sbjct: 181 KQDSI-NLEEILAWKKHVGKGFKVYSFEGDHFFINNNVKNINSIINSTLI 229 >C3AE80.1 C3AE80_BACMY Thioesterase Length = 240 Score = 209 bits (533), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 98/231 (42%), Positives = 152/231 (65%) Query: 8 LDGMKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDIS 67 ++ KLFC P+AGGSA ++KWK + IE++P+ELAGRG R E Y S+ + V D+ Sbjct: 1 MNKTKLFCFPHAGGSAFSYSKWKNYFNPHIEVVPIELAGRGYRIEESLYQSMEEAVNDVY 60 Query: 68 TAIMDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQDKKKIHH 127 +I+ Q+ + Y L+GHSMG+L EL ++IQ + P L LSG + P+ + K H+ Sbjct: 61 NSIVKQLDNSPYILYGHSMGSLIAYELARKIQDSNNVLPEFLVLSGRNHPNSKIKNIRHN 120 Query: 128 LPDDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVL 187 LPD++F EVI +GGT +V +++ELM IF P+LR+D+K VE+YI D+D VL Sbjct: 121 LPDEQFKNEVIAMGGTSTEVLQSEELMGIFLPILRADFKIVETYIHNNNIRACDIDFLVL 180 Query: 188 VGDEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTL 238 G +DE+T+ ++++W +YT++SC+ +F+G HFF+NE +++ I R L Sbjct: 181 NGKKDELTTYDEVKKWKQYTSKSCTFNSFEGNHFFLNENIEEIASGIIRKL 231 >Q2XP41.1 Q2XP41_BACSU SubT Length = 234 Score = 209 bits (531), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 102/231 (44%), Positives = 148/231 (64%), Gaps = 2/231 (0%) Query: 11 MKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDISTAI 70 MKLFCLPYAGGS + F WK + IE+ P++L GRGRRF EP Y SL + V DI + Sbjct: 1 MKLFCLPYAGGSESAFYSWKGHMQPDIEIYPIQLKGRGRRFNEPCYESLEEAVQDIFEQV 60 Query: 71 MDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPH-IQDKKKIHHLP 129 + Y+LFGHSMG+L EL Q+ G EKP H+F SG P+ I+ +K+H LP Sbjct: 61 QAERKGDDYALFGHSMGSLLAYELYYQMSGAGAEKPVHIFFSGYKAPNRIRKTEKLHTLP 120 Query: 130 DDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVLVG 189 + F ++++ LGGTP++++ ++EL E+F P+L+SD+K VE+YI + K D DITVL G Sbjct: 121 NPIFKKKIVELGGTPEELTNHEELFELFIPILKSDFKMVENYIYQERNSKIDCDITVLNG 180 Query: 190 DEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTLIA 240 ED + S D+ W +T+ + F+G HFF++ +K+ ++IN +L A Sbjct: 181 KEDAM-SKEDVSDWKHHTSGHFTAYYFEGNHFFLHHHVEKITEIINHSLTA 230 >C3BKA1.1 C3BKA1_9BACI Thioesterase Length = 240 Score = 208 bits (530), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 98/233 (42%), Positives = 155/233 (66%) Query: 8 LDGMKLFCLPYAGGSATVFTKWKRELDSRIELIPVELAGRGRRFVEPFYPSLHDGVMDIS 67 + +KLFCLP+AGGSA ++KWK+ + IE+IP+ELAGRG R E Y S+ + V D+ Sbjct: 1 MTKIKLFCLPHAGGSAFNYSKWKKYFNPFIEVIPIELAGRGYRIEESLYHSIEEAVNDVY 60 Query: 68 TAIMDQIGDKGYSLFGHSMGTLYICELMKQIQANGWEKPRHLFLSGMHPPHIQDKKKIHH 127 I+ Q+ + Y L+GHSMG+L EL ++IQ + P L LSG + P+ + K H+ Sbjct: 61 KIIIKQLNNSPYILYGHSMGSLIAYELARKIQISNDVLPEFLVLSGRNHPNSKIKDIRHN 120 Query: 128 LPDDEFWQEVIRLGGTPKQVSENKELMEIFTPVLRSDYKNVESYITEAPQEKWDMDITVL 187 L +++F EVI +GGTP +V +++ELMEIF P+LR+D+K VE+YI + D+D +L Sbjct: 121 LSNEQFKNEVIAMGGTPSEVFQSEELMEIFLPILRADFKIVETYIHNNNIQVCDIDFLIL 180 Query: 188 VGDEDEITSMGDIRQWNEYTTQSCSIVTFQGGHFFINEEEKKVIDLINRTLIA 240 G DE+TS ++++W +YT + C+ +F+G HFF++E +++ +I R L++ Sbjct: 181 NGKNDELTSYEEVKKWEQYTNKMCTFHSFEGKHFFLHENIEEIASIIIRKLVS 233 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,583,707,507 Number of extensions: 67101721 Number of successful extensions: 152861 Number of sequences better than 1.0e-05: 799 Number of HSP's gapped: 151075 Number of HSP's successfully gapped: 800 Length of query: 240 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 108 Effective length of database: 1,817,122,223 Effective search space: 196249200084 Effective search space used: 196249200084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)