BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0010.p (425 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B9E2D2.1 B9E2D2_CLOK1 Putative uncharacterized protein 387 e-105 A5N8Z3.1 A5N8Z3_CLOK5 Predicted PKS acyltransferase domain 387 e-105 B8I5H7.1 B8I5H7_CLOCE Malonyl CoA-acyl carrier protein transacylase 384 e-105 C3HA40.1 C3HA40_BACTU PKS acyltransferase domain 317 2e-84 B8I5C8.1 B8I5C8_CLOCE Malonyl CoA-acyl carrier protein transacylase 313 2e-83 >B9E2D2.1 B9E2D2_CLOK1 Putative uncharacterized protein Length = 424 Score = 387 bits (994), Expect = e-105, Method: Compositional matrix adjust. Identities = 188/419 (44%), Positives = 277/419 (66%), Gaps = 8/419 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M K+ALLFPGQGSQY MGK YE A+E F ND+LG+D+ + EGN+++LT TE Sbjct: 1 MKKVALLFPGQGSQYIGMGKKFYEGYPIAKETFEEANDVLGFDIRKLCFEGNMEKLTMTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 QPAI A+F+ + PS++AGHSLGEITAL+CAG ++ A+++V R Sbjct: 61 NTQPAILTMGVAMFRVYMQEIGIEPSYLAGHSLGEITALSCAGAIQFQDAVKMVRNRGKF 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ+AT G G MAAI+GL +E+EC +V++NYN+ +QVVISG K V + Sbjct: 121 MQEATAFGTGSMAAISGLSWKEIEDECKIKREWGKLVVISNYNAWDQVVISGYKDAVEEV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 A NL + G IPL VSAPFH+P ++++++ F+ L + S + WPV+SN+ A PY D Sbjct: 181 AANLYEKGGGIIPLNVSAPFHSPLMKLASEKFENELTKYEYSKLKWPVMSNITALPYEDS 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYE-E 299 I L Q+V V+W+ TI+YL + V IELGPK+ L NL+K++ ++ +SY+ E Sbjct: 241 SRIVKNLKKQIVEPVKWQATIEYLEKNKVEIGIELGPKTVLKNLMKKNSKRIKVYSYDKE 300 Query: 300 HNQEIMAI---IDEQIA----EKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQ 352 + +++ ++ I + + ++++RS+AIAVCT+N NWD+ EY++GVI+PYRKV+ Sbjct: 301 EDVQVLKTELNMNRNIGSIEKDNKMSVITRSMAIAVCTKNNNWDDKEYEKGVIQPYRKVK 360 Query: 353 ALSNRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQRERFTQIFDETKNRHLFDDF 411 + +E+E +E + + M+EA++M+RSVF+TK+ P +EQ ERF QIF+ET RHLF DF Sbjct: 361 EIQAEIEKEEKEPTIEQMREALKMMRSVFITKRTPQKEQIERFNQIFNETGTRHLFSDF 419 >A5N8Z3.1 A5N8Z3_CLOK5 Predicted PKS acyltransferase domain Length = 424 Score = 387 bits (994), Expect = e-105, Method: Compositional matrix adjust. Identities = 188/419 (44%), Positives = 277/419 (66%), Gaps = 8/419 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M K+ALLFPGQGSQY MGK YE A+E F ND+LG+D+ + EGN+++LT TE Sbjct: 1 MKKVALLFPGQGSQYIGMGKKFYEGYPIAKETFEEANDVLGFDIRKLCFEGNMEKLTMTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 QPAI A+F+ + PS++AGHSLGEITAL+CAG ++ A+++V R Sbjct: 61 NTQPAILTMGVAMFRVYMQEIGIEPSYLAGHSLGEITALSCAGAIQFQDAVKMVRNRGKF 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ+AT G G MAAI+GL +E+EC +V++NYN+ +QVVISG K V + Sbjct: 121 MQEATAFGTGSMAAISGLSWKEIEDECKIKREWGKLVVISNYNAWDQVVISGYKDAVEEV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 A NL + G IPL VSAPFH+P ++++++ F+ L + S + WPV+SN+ A PY D Sbjct: 181 AANLYEKGGGIIPLNVSAPFHSPLMKLASEKFENELTKYEYSKLKWPVMSNITALPYEDS 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYE-E 299 I L Q+V V+W+ TI+YL + V IELGPK+ L NL+K++ ++ +SY+ E Sbjct: 241 SRIVKNLKKQIVEPVKWQATIEYLEKNKVEIGIELGPKTVLKNLMKKNSKRIKVYSYDKE 300 Query: 300 HNQEIMAI---IDEQIA----EKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQ 352 + +++ ++ I + + ++++RS+AIAVCT+N NWD+ EY++GVI+PYRKV+ Sbjct: 301 EDVQVLKTELNMNRNIGSIEKDNKMSVITRSMAIAVCTKNNNWDDKEYEKGVIQPYRKVK 360 Query: 353 ALSNRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQRERFTQIFDETKNRHLFDDF 411 + +E+E +E + + M+EA++M+RSVF+TK+ P +EQ ERF QIF+ET RHLF DF Sbjct: 361 EIQAEIEKEEKEPTIEQMREALKMMRSVFITKRTPQKEQIERFNQIFNETGTRHLFSDF 419 >B8I5H7.1 B8I5H7_CLOCE Malonyl CoA-acyl carrier protein transacylase Length = 423 Score = 384 bits (987), Expect = e-105, Method: Compositional matrix adjust. Identities = 185/418 (44%), Positives = 274/418 (65%), Gaps = 7/418 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M I LLFPGQGSQY MGK LY++ + A+ +F ND LG+DL + G+LD+LT+TE Sbjct: 1 MKNITLLFPGQGSQYEGMGKKLYDNYSVAKHVFDEANDALGFDLKELCFSGSLDELTKTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S A ++ K P++ AGHSLGE++AL CAG+F++ ++++V +R L Sbjct: 61 NAQPAILTCSIAAYEVYMKEFGILPTYYAGHSLGELSALACAGIFSFADSVKIVRQRGRL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ+A G G MAA++G+ +++ +C SND + +V++NYNS +Q+VISG K+ V + Sbjct: 121 MQEAAASGVGAMAAVSGIDKNILQAKCEEYSNDNNIVVISNYNSPSQIVISGHKSAVEKI 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + L I A+ +PL+VSAPFH+P +E F+ VL + V+SNV P+ Sbjct: 181 SLELQGINAMVVPLKVSAPFHSPLMETIKGKFREVLESYPCGDFKGTVISNVTGLPHKSK 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYE-- 298 + + L+ Q+V V+W+ T+ +L GV T +E+GPK+ L NL ++ ++AFSY+ Sbjct: 241 DILVDLLTEQLVQPVQWESTMKFLYENGVRTVMEIGPKTVLANLAIKNIPDIKAFSYDKP 300 Query: 299 EHNQEIMAIIDEQIAEK-----RPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQA 353 E ++ II + + +PT+V+R LAIAVCT+N+NW+ EYQ+GVIEPYR++Q Sbjct: 301 EDIEKFRKIITNNTSNRSENLPQPTVVTRCLAIAVCTKNKNWNNEEYQKGVIEPYRRIQR 360 Query: 354 LSNRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQRERFTQIFDETKNRHLFDDF 411 L L++ G + S + MKEA+EML+SVF TKK P++EQ ER+ Q+FDET RHLF DF Sbjct: 361 LQEELDKTGDKPSLEQMKEALEMLKSVFETKKTPVQEQVERYNQVFDETGTRHLFADF 418 >C3HA40.1 C3HA40_BACTU PKS acyltransferase domain Length = 418 Score = 317 bits (811), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 170/418 (40%), Positives = 247/418 (59%), Gaps = 9/418 (2%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M K+ LF GQGSQY MGK+LY + A A+E + +++LG+DL + EGN ++L++TE Sbjct: 1 MSKLGFLFSGQGSQYIGMGKNLYNNFAIAKETYDEASEVLGFDLGKLCFEGNANELSQTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 QPAI S A F+ + P ++AGHSLGE TAL CAGV + A++LV R Sbjct: 61 NTQPAILTTSMAQFRVYMEEIGEEPHYLAGHSLGEYTALVCAGVIDFSDAVKLVRARGMF 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 M++ +G G MA I + +VEEE ++S I ++NYN VVISG V Q Sbjct: 121 MKEIANDGLGAMAVIRNIDEKIVEEESYKISLGGRVIGISNYNGEKDVVISGEAEAVVQV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 E L GA L VSA FH+P +E++ + F+ L + +LSNV A PY D Sbjct: 181 CEELKSKGAEIFKLNVSAAFHSPIMELAAEKFKNELNRYAFKDPKYKILSNVTALPYEDK 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEEH 300 + I L Q+V V W + YL +Q ++ IE GPK+ L NL++ + S++ A+SY++ Sbjct: 241 DQILDILGEQIVKPVNWHAIMKYLGQQELDNVIEFGPKATLRNLVRNNISNIGAYSYDK- 299 Query: 301 NQEIMAIIDEQIAEK-------RPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQA 353 +E + + E+I K + +++SRSL IAV T+N NW+ EY RGVI+PY K+ Sbjct: 300 -KEDVKEMKEKIFAKEVSHQKNKYSIISRSLGIAVATKNNNWNNEEYMRGVIKPYEKIAM 358 Query: 354 LSNRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQRERFTQIFDETKNRHLFDDF 411 L LEE + + D M+ A+EML+SVF TK P +EQ+ERF Q+F+ET + F++F Sbjct: 359 LQTELEETNKMPTIDQMQMAVEMLKSVFQTKGTPKDEQQERFNQLFEETNTKRYFENF 416 >B8I5C8.1 B8I5C8_CLOCE Malonyl CoA-acyl carrier protein transacylase Length = 409 Score = 313 bits (802), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 161/410 (39%), Positives = 251/410 (61%), Gaps = 13/410 (3%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M KIA LFPGQG+QY MG L E+ A ++F N+ LG DL + +G++D LT+TE Sbjct: 1 MNKIAFLFPGQGAQYIGMGSKLCENFTVAGQVFDEANEALGLDLKKLCFQGSMDDLTKTE 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 QPAI S A F+ + P + AGHSLGE++AL CAG + A+ LV +R Sbjct: 61 NTQPAILTVSVAAFRVYMQEIGVKPDYAAGHSLGELSALCCAGGIEFTDAVSLVRQRGRF 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ+A EG G MAA++G+ ++E EC+ +S + +VV+NYNS Q VISG V Sbjct: 121 MQEAVPEGTGSMAAVSGVEKEIIERECSLVSKGGNIVVVSNYNSLEQTVISGHVKAVNDV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 E L +GA + L+VSAPFH+P ++ + F+ VL + WPV+SNV ++PY Sbjct: 181 GEQLKSMGARVVYLKVSAPFHSPLMQPAADRFRDVLKKYSFGEMKWPVISNVTSEPYPGK 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEEH 300 + I LS+Q+ + VRW+ T++YL G++ +E+GPK+ L NL++ + S + FS E Sbjct: 241 DKIIDYLSLQITNPVRWQSTMEYLQNSGIDKAVEMGPKAVLKNLLRSNKS-ITVFSSE-- 297 Query: 301 NQEIMAIIDEQI-----AEKRPT-----LVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRK 350 E +++I ++ K+P +++R +A AV T+N+NWDE YQ+GV+EPYRK Sbjct: 298 TTEDISLIKKKFDSDASGSKKPANNGLMVITRCIAAAVSTKNRNWDENAYQKGVVEPYRK 357 Query: 351 VQALSNRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQRERFTQIFD 400 + + L ++ + + + ++EA+++L+ +F TK + +EEQ ER +Q+F+ Sbjct: 358 LVEMKEELVKQRIQPTNEHIEEAVDLLKQIFNTKLLTIEEQNERLSQLFN 407 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,259,689,538 Number of extensions: 85925918 Number of successful extensions: 285878 Number of sequences better than 1.0e-05: 4427 Number of HSP's gapped: 273881 Number of HSP's successfully gapped: 5138 Length of query: 425 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 287 Effective length of database: 1,760,596,133 Effective search space: 505291090171 Effective search space used: 505291090171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)