BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0009.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase 199 2e-49 B9E3Q1.1 B9E3Q1_CLOK1 Putative uncharacterized protein 189 2e-46 A5MZS1.1 A5MZS1_CLOK5 Predicted hybrid nonribosomal peptide synt... 189 2e-46 A6LQ11.1 A6LQ11_CLOB8 Erythronolide synthase., Aspartate racemas... 187 6e-46 B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein 185 3e-45 >B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase Length = 1613 Score = 199 bits (505), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 98/206 (47%), Positives = 137/206 (66%), Gaps = 13/206 (6%) Query: 34 DIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQAA 93 DIA++G+A KLP A N E+W NI GRD + P RK+D + ++ + + + + Sbjct: 39 DIAIVGMAAKLPFAPNIDEFWINIRNGRDCVSDFPEERKKDTDALLGHM---NLKPSEIE 95 Query: 94 YAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTGV 153 Y+ +LD +D FD ++F +SPKEA L+DPNQRLFLQ +A+ED+GYG G+L G+ TGV Sbjct: 96 YSIGAFLDEVDKFDYKFFKLSPKEASLIDPNQRLFLQTAYQAIEDSGYGNGRLSGSKTGV 155 Query: 154 FLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSSS 213 ++GYSSDFG YK + L+ +A+ GNIKSII +R++Y+LD+ GPSMLVDTACSSS Sbjct: 156 YVGYSSDFGESYKRFIHTVDPGLSGIAIPGNIKSIIASRISYYLDLKGPSMLVDTACSSS 215 Query: 214 LVAXXXXSSRLPVHPP-RRVRDGICR 238 LVA +H R +R+G C Sbjct: 216 LVA---------IHMACRAIRNGECE 232 >B9E3Q1.1 B9E3Q1_CLOK1 Putative uncharacterized protein Length = 1190 Score = 189 bits (479), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 89/185 (48%), Positives = 128/185 (69%), Gaps = 4/185 (2%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG+A P A + E+W+N+ G+D I P RK+D ++ ++ + KD+ Sbjct: 28 KDIAIIGMAATFPKAEDLNEFWSNLVDGKDCIGNFPEKRKEDIDKYLNYI---GMSKDKI 84 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 Y ++GYLD ID FDC +F ++PKEA LM+P+QRLFLQ + +EDAGYG +L G+ TG Sbjct: 85 KYLQAGYLDEIDKFDCRFFRLTPKEASLMNPSQRLFLQTAWKTIEDAGYGGNRLVGSNTG 144 Query: 153 VFLGYSSDFGGE-YKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACS 211 VF+GY DF G+ Y ++S + +++ GN+ SIIP+R++Y LD+ GPSML+DTACS Sbjct: 145 VFVGYIGDFQGDKYGDMISRIDESNSAISIVGNLSSIIPSRISYMLDLKGPSMLIDTACS 204 Query: 212 SSLVA 216 SSLVA Sbjct: 205 SSLVA 209 >A5MZS1.1 A5MZS1_CLOK5 Predicted hybrid nonribosomal peptide synthetase/polyketide synthase Length = 1190 Score = 189 bits (479), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 89/185 (48%), Positives = 128/185 (69%), Gaps = 4/185 (2%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG+A P A + E+W+N+ G+D I P RK+D ++ ++ + KD+ Sbjct: 28 KDIAIIGMAATFPKAEDLNEFWSNLVDGKDCIGNFPEKRKEDIDKYLNYI---GMSKDKI 84 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 Y ++GYLD ID FDC +F ++PKEA LM+P+QRLFLQ + +EDAGYG +L G+ TG Sbjct: 85 KYLQAGYLDEIDKFDCRFFRLTPKEASLMNPSQRLFLQTAWKTIEDAGYGGNRLVGSNTG 144 Query: 153 VFLGYSSDFGGE-YKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACS 211 VF+GY DF G+ Y ++S + +++ GN+ SIIP+R++Y LD+ GPSML+DTACS Sbjct: 145 VFVGYIGDFQGDKYGDMISRIDESNSAISIVGNLSSIIPSRISYMLDLKGPSMLIDTACS 204 Query: 212 SSLVA 216 SSLVA Sbjct: 205 SSLVA 209 >A6LQ11.1 A6LQ11_CLOB8 Erythronolide synthase., Aspartate racemase EC=2.3.1.94 EC=5.1.1.13 Length = 1225 Score = 187 bits (476), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 94/184 (51%), Positives = 124/184 (67%), Gaps = 9/184 (4%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG+AGK P A+N EYW NI G+D I+ P SRK+D E C+K++ Sbjct: 25 KDIAIIGIAGKWPLANNVEEYWENIKNGKDCIQNFPYSRKKDIEPF--------CEKNKE 76 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 Y E G+L+ ID FD +F ISPK+A LMDP+ RLFL+ EA+EDAGYG K+ GT TG Sbjct: 77 -YFECGFLNEIDKFDYSFFHISPKDANLMDPHHRLFLESAYEAIEDAGYGGKKIIGTNTG 135 Query: 153 VFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSS 212 VF+G S +Y L++ E A++ N K+I+P+R++Y D+HGPSML+DTACSS Sbjct: 136 VFVGMSDLGDIQYIDLINKSEPEFLVQALSSNTKAILPSRVSYTFDLHGPSMLIDTACSS 195 Query: 213 SLVA 216 SLVA Sbjct: 196 SLVA 199 >B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein Length = 1009 Score = 185 bits (469), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 85/185 (45%), Positives = 123/185 (66%) Query: 32 RKDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQ 91 R DIA+IG++G+ +A N +EYW + GRD IR+ P R + + L K+ Sbjct: 29 RHDIAIIGISGRFAEAENLKEYWNILKNGRDCIRKFPEDRASEITNYVEQLYSLGLSKEG 88 Query: 92 AAYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPT 151 + E+G+L+ +D FD +F +SP+EA LMDPNQR+FL+ A+EDAGY K+ GT T Sbjct: 89 MKFIEAGFLNHVDQFDYNFFGLSPREASLMDPNQRIFLETVWSAIEDAGYNSKKIRGTKT 148 Query: 152 GVFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACS 211 GV+LG+S+DF +Y+ + S+E ++ GNIKSII +R++Y LD GPS++VDTACS Sbjct: 149 GVYLGFSNDFHDDYRKYVQTMSSESIGISTAGNIKSIIASRISYLLDFKGPSLVVDTACS 208 Query: 212 SSLVA 216 S+LVA Sbjct: 209 SALVA 213 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,354,104,639 Number of extensions: 52629910 Number of successful extensions: 136292 Number of sequences better than 1.0e-05: 4782 Number of HSP's gapped: 128488 Number of HSP's successfully gapped: 5955 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)