BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0008.p (430 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I978.1 B8I978_CLOCE Beta-ketoacyl synthase 391 e-107 B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein 390 e-106 B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein 387 e-105 A5N8Z2.1 A5N8Z2_CLOK5 Predicted polyketide synthase 387 e-105 B8I5I0.1 B8I5I0_CLOCE Condensation domain protein 386 e-105 >B8I978.1 B8I978_CLOCE Beta-ketoacyl synthase Length = 1272 Score = 391 bits (1004), Expect = e-107, Method: Compositional matrix adjust. Identities = 192/402 (47%), Positives = 266/402 (66%), Gaps = 20/402 (4%) Query: 13 IGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQSI 72 GI +S R +TFD ++DGTG GEG LLK +A++D D+I+A+IKGSA+NQDG+SI Sbjct: 239 FGINASDGRAKTFDDSSDGTGSGEGVITYLLKPLSRALKDKDNIHAVIKGSAINQDGRSI 298 Query: 73 GITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRKK 132 G++APN AQE++I+ AWKDA + PET+S IEAHGT T+LGDP+E+DGI+RAF RYT KK Sbjct: 299 GLSAPNVLAQEDVIVSAWKDAGIDPETVSYIEAHGTGTKLGDPIEVDGISRAFRRYTSKK 358 Query: 133 QFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVND 192 QFC+IGS+KTNIGHLD+AAG++G+LK +L+L+++ LP ++H++ PN I+F DSPVYVND Sbjct: 359 QFCSIGSVKTNIGHLDNAAGVAGMLKAVLSLKNKQLPASLHYKVPNRRINFKDSPVYVND 418 Query: 193 AVRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAVERTGNLSADR--WNMLTLSAK 250 + WE+ PRRCG++SFG SGTNCH+ILE+ N S DR +LTLSAK Sbjct: 419 KLTPWETHGFPRRCGVSSFGFSGTNCHIILEEAPSVV------NKSIDRPCLEVLTLSAK 472 Query: 251 NSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQSLK 310 +D L+ LV Y +I ++E VD+ N+CYT NTGR H ++ A++ RN ++L KL L Sbjct: 473 TNDALKELVKRYRLYIQKNEDVDIGNMCYTQNTGRWHFDYRIAVIARNTIELKNKLDKLV 532 Query: 311 QAGTGKISMEHVYVRPGHEQANAKIPGSSPDVQGIVQEMRAAVDQH----------RGSL 360 I+ + +Y G + N K G + + ++E+ D+ + Sbjct: 533 SIDFEDINEKEIYF--GIQLDNIKQKGQNGLFESKIKELSCEADKKLEEFILSEKTEEDV 590 Query: 361 LQQLAQLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWI 402 L + + YV+G I W LY ISLP YPF+ TRCWI Sbjct: 591 LYDICKRYVEGANIDWDILYKGEDRTRISLPTYPFRRTRCWI 632 >B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein Length = 3695 Score = 390 bits (1003), Expect = e-106, Method: Compositional matrix adjust. Identities = 193/414 (46%), Positives = 264/414 (63%), Gaps = 15/414 (3%) Query: 3 LQPFDKS-STGIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIK 61 L P D + GIGI SS TR+FD+ +DGTG GEG ++LK ++AI+D D+IY++IK Sbjct: 235 LLPVDNNPQNGIGIESSSGVTRSFDEYSDGTGFGEGVAAIVLKPLKKAIEDRDNIYSIIK 294 Query: 62 GSAVNQDGQSIGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGI 121 GSAVNQDG S+GIT PN++AQ ++IIRAWKDA++ PETIS IEAHGT T LGDPVEIDGI Sbjct: 295 GSAVNQDGTSMGITVPNAQAQSDVIIRAWKDAKIDPETISYIEAHGTGTNLGDPVEIDGI 354 Query: 122 TRAFDRYTRKKQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNI 181 AF +YT KKQFCA+ S+KTNIGHLD AAGI+GLLK +L+L+H+ L P++HF PN I Sbjct: 355 EMAFAQYTNKKQFCAVSSVKTNIGHLDSAAGIAGLLKAVLSLKHKELFPSLHFDRPNQKI 414 Query: 182 SFPDSPVYVNDAVRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAVERTGNLSADR 241 +F +SPVY+ND++ WE+ PRRCGI+SFGLSGTNCH++LE+ + ++ Sbjct: 415 NFQNSPVYINDSLEQWENHGGPRRCGISSFGLSGTNCHLVLEEPPQIKSIPEES--PGCN 472 Query: 242 WNMLTLSAKNSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMD 301 +++ TLS++N + L + Y +++N+ V L +LC T NTGR H+ + A V ++ + Sbjct: 473 YHIFTLSSQNINGLMEQLKNYEKYLNREREVSLGSLCSTTNTGRGHYNFRLAFVITSKNE 532 Query: 302 LLEKLQSLKQAGTGKISMEHVYV------------RPGHEQANAKIPGSSPDVQGIVQEM 349 L EK+Q L G VY R +E + +I S + + E Sbjct: 533 LKEKIQKLSTGGLENFEKYGVYFGKHKIVDKEKESRGNNEYSQEEIADLSLQTETYLNEA 592 Query: 350 RAAVDQHRGSLLQQLAQLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIE 403 Q + L+ + LYV G + W LY G Y +SLP Y F + RCWIE Sbjct: 593 INNESQQKKVSLENICALYVNGANVQWNRLYKSGSYAKVSLPGYSFMKKRCWIE 646 >B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein Length = 1009 Score = 387 bits (993), Expect = e-105, Method: Compositional matrix adjust. Identities = 191/414 (46%), Positives = 282/414 (68%), Gaps = 22/414 (5%) Query: 8 KSSTGIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQ 67 ++S GIG SS FR +TFD ++DG GLGEGAGVV+LK +A+ DGD+I+A+IKGSA+NQ Sbjct: 244 EASWGIGTQSSDFRCKTFDNSSDGIGLGEGAGVVILKPLYKALNDGDNIHAVIKGSAINQ 303 Query: 68 DGQSIGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDR 127 DG S+G+T PN AQEE+II+AW+D+ V+PE+IS IEAHGT T+LGDP+EI G+ RAF + Sbjct: 304 DGNSLGLTTPNPIAQEEVIIKAWEDSHVNPESISYIEAHGTGTKLGDPIEISGVQRAFKK 363 Query: 128 YTRKKQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSP 187 YT KKQFCAIGS+KTN+GHLD +GI+ ++K ILAL+++ LPP+I+F++PN I+F +SP Sbjct: 364 YTNKKQFCAIGSVKTNVGHLDCCSGIASMMKVILALKNKKLPPSIYFKTPNSKINFEESP 423 Query: 188 VYVNDAVRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAVERTG-NLSADRWNMLT 246 VYVND WES ++P RCGI+SFGLSGTNCH+ILE E +++ N + N+LT Sbjct: 424 VYVNDRFIDWES-DYPLRCGISSFGLSGTNCHIILE---EAPVIKKNQINKTTQGLNVLT 479 Query: 247 LSAKNSDVLRRLVSEYHQFINQ-SESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEK 305 +SAK+ + L+ L+ Y++F N+ S+ + +DN+ YTAN GR+H ++ AI+F D+ K Sbjct: 480 ISAKSIESLKELIRRYNEFFNEKSDKISMDNIIYTANAGRNHFNYRLAILFDGFQDIKNK 539 Query: 306 LQSLKQAG--------TGKISMEHVYVRPGHEQ------ANAKIPGSSPDVQGIVQEMRA 351 L +L G E +V+ G ++ A K+ ++ + + E+ Sbjct: 540 LATLCLVDLNENLLNPKGIYFGEVRFVKGGRKKHKNELTAEEKVDMTNKADEKL--EIYM 597 Query: 352 AVDQHRGSLLQQLAQLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIEPK 405 + +++ +L +L +LY G I W+ +Y + + +SLP YPF + RCW+ + Sbjct: 598 SCRKNQSEVLGELCKLYTHGADIHWEKVYENKKVTRVSLPTYPFLQKRCWVRSR 651 >A5N8Z2.1 A5N8Z2_CLOK5 Predicted polyketide synthase Length = 1009 Score = 387 bits (993), Expect = e-105, Method: Compositional matrix adjust. Identities = 191/414 (46%), Positives = 282/414 (68%), Gaps = 22/414 (5%) Query: 8 KSSTGIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQ 67 ++S GIG SS FR +TFD ++DG GLGEGAGVV+LK +A+ DGD+I+A+IKGSA+NQ Sbjct: 244 EASWGIGTQSSDFRCKTFDNSSDGIGLGEGAGVVILKPLYKALNDGDNIHAVIKGSAINQ 303 Query: 68 DGQSIGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDR 127 DG S+G+T PN AQEE+II+AW+D+ V+PE+IS IEAHGT T+LGDP+EI G+ RAF + Sbjct: 304 DGNSLGLTTPNPIAQEEVIIKAWEDSHVNPESISYIEAHGTGTKLGDPIEISGVQRAFKK 363 Query: 128 YTRKKQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSP 187 YT KKQFCAIGS+KTN+GHLD +GI+ ++K ILAL+++ LPP+I+F++PN I+F +SP Sbjct: 364 YTNKKQFCAIGSVKTNVGHLDCCSGIASMMKVILALKNKKLPPSIYFKTPNSKINFEESP 423 Query: 188 VYVNDAVRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAVERTG-NLSADRWNMLT 246 VYVND WES ++P RCGI+SFGLSGTNCH+ILE E +++ N + N+LT Sbjct: 424 VYVNDRFIDWES-DYPLRCGISSFGLSGTNCHIILE---EAPVIKKNQINKTTQGLNVLT 479 Query: 247 LSAKNSDVLRRLVSEYHQFINQ-SESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEK 305 +SAK+ + L+ L+ Y++F N+ S+ + +DN+ YTAN GR+H ++ AI+F D+ K Sbjct: 480 ISAKSIESLKELIRRYNEFFNEKSDKISMDNIIYTANAGRNHFNYRLAILFDGFQDIKNK 539 Query: 306 LQSLKQAG--------TGKISMEHVYVRPGHEQ------ANAKIPGSSPDVQGIVQEMRA 351 L +L G E +V+ G ++ A K+ ++ + + E+ Sbjct: 540 LATLCLVDLNENLLNPKGIYFGEVRFVKGGRKKHKNELTAEEKVDMTNKADEKL--EIYM 597 Query: 352 AVDQHRGSLLQQLAQLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIEPK 405 + +++ +L +L +LY G I W+ +Y + + +SLP YPF + RCW+ + Sbjct: 598 SCRKNQSEVLGELCKLYTHGADIHWEKVYENKKVTRVSLPTYPFLQKRCWVRSR 651 >B8I5I0.1 B8I5I0_CLOCE Condensation domain protein Length = 2006 Score = 386 bits (992), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/415 (46%), Positives = 270/415 (65%), Gaps = 22/415 (5%) Query: 10 STGIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDG 69 S +GI SS + RTFD N+DGTG+GEG VLLK QA+ D DHIYA+IKGSA N DG Sbjct: 241 SENVGIASSDGKARTFDDNSDGTGIGEGVAAVLLKPLEQALNDKDHIYAVIKGSAANNDG 300 Query: 70 QSIGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYT 129 SIGITAPNS AQ + I++AW+D+ ++PET+S IEAHGT T+LGDP+EIDGI RAF +YT Sbjct: 301 SSIGITAPNSLAQRDAIVKAWEDSGINPETLSYIEAHGTGTKLGDPIEIDGIKRAFKKYT 360 Query: 130 RKKQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVY 189 KKQFCAIGS+K+NIGHLD+ AGI+GL+K +L +Q+ +PPT+HF+ PN I F DSPVY Sbjct: 361 DKKQFCAIGSVKSNIGHLDNVAGIAGLIKAVLCIQYGQIPPTLHFKRPNRKIMFEDSPVY 420 Query: 190 VNDAVRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAVERTGNLSADRWNMLTLSA 249 VND + WE+ +PRRCGI+SFG+SGTNCHMILE+ + ++ N + + +LTLSA Sbjct: 421 VNDRLHEWETMGYPRRCGISSFGISGTNCHMILEEAPNSKG--KSENPQS-KLQVLTLSA 477 Query: 250 KNSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQSL 309 KN L L+ + +++ +++ V ++++C+T NTGR HH + A++ NR D+ EK+ L Sbjct: 478 KNEKSLYSLLKSFDEYLKKNKEVSIEDICFTQNTGRMHHSLRIALILENRHDMEEKISKL 537 Query: 310 KQAGTGKISMEHVYVRPGHEQANAKIPG-------SSPDVQGI-------VQEMRAAVDQ 355 I + VY G+ + G S D + + ++E+ + + Sbjct: 538 ADLQPKGIREQGVYY--GYHKVVRNSEGIKNDGYISETDKRELSKTASERLKELVTSGNY 595 Query: 356 HRGSLLQQLAQLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIEPKAYMPK 410 +RG+ L+++ LY G I W LY +SLP YPF R WI+ K M + Sbjct: 596 NRGT-LEEICILYTTGGDIDWDFLYKSE--NKVSLPTYPFNRKRFWIDTKRQMKE 647 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,623,538,025 Number of extensions: 110874723 Number of successful extensions: 280160 Number of sequences better than 1.0e-05: 9255 Number of HSP's gapped: 265882 Number of HSP's successfully gapped: 10486 Length of query: 430 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 291 Effective length of database: 1,751,175,118 Effective search space: 509591959338 Effective search space used: 509591959338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)