BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0005.p (541 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I5H3.1 B8I5H3_CLOCE Beta-ketoacyl synthase 488 e-136 C3HA42.1 C3HA42_BACTU Beta-ketoacyl synthase 469 e-130 B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein 463 e-128 C3HA44.1 C3HA44_BACTU Hybrid nonribosomal peptide synthetase/pol... 459 e-127 B8I979.1 B8I979_CLOCE Amino acid adenylation domain protein 450 e-124 >B8I5H3.1 B8I5H3_CLOCE Beta-ketoacyl synthase Length = 1620 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 239/495 (48%), Positives = 334/495 (67%), Gaps = 4/495 (0%) Query: 2 YTHLLDQTDELALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNE 61 Y +++ D LA+TGS++GIL+SRISY L+L GP++V+D+ACSSGL+A+H+AC A++ + Sbjct: 166 YKYMIQDRDPLAITGSWSGILASRISYILNLNGPALVIDTACSSGLVAVHEACNALKTGK 225 Query: 62 CDMAIAGGVCLIFFPMKNPAFGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVE 121 C MAIAGG+ +I+ P++N G+VES ++ FD +A GT+WGEG+G ++LKPL KA+E Sbjct: 226 CQMAIAGGIHIIYKPIENNEMGIVESNDSTIRAFDDKASGTVWGEGVGVVILKPLKKAME 285 Query: 122 DRDPIHAVIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTV 181 D+D I+AVIKGSAVNNDG SNG+TAP A AQEDL +AW A I PE I + E HGTGTV Sbjct: 286 DKDEIYAVIKGSAVNNDGASNGITAPKAEAQEDLLIKAWKEARINPENISYIETHGTGTV 345 Query: 182 LGDPIEVQSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVST 241 LGDPIE++ LS AFRK+T K QFCGIGSVK+N+GHL ASGIAS++KT+L++ + + T Sbjct: 346 LGDPIEIKGLSRAFRKFTKKNQFCGIGSVKTNLGHLVGASGIASLIKTVLAINKKVIPPT 405 Query: 242 LHFDDPNPFIDFVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAPVSP 301 L+F+ PN +IDF +PVY+ +K W+ A ++I L G+SSFGFSGTN H+V++E Sbjct: 406 LNFEYPNRYIDFSNAPVYVNDKTTFWE-ADQNIRL-CGVSSFGFSGTNCHVVIQEVKKEI 463 Query: 302 KLAPASRPYHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLA 361 + AS +FT+SAK + + E V RYL+ + R L D+C T A GRGHY YR A Sbjct: 464 RAETASHRPMVFTLSAKSQFSMKELVQRYLKFINRPEVPSLKDICSTTAVGRGHYNYRAA 523 Query: 362 LIVRDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHE 421 +IVR + EL+EKL ++ + I IY+GE KIV K +ITE + + Sbjct: 524 IIVRGMDELREKLIQLREVIDIKQTHVQNIYFGEHKIVSSKKGLNEANDITEHERREISQ 583 Query: 422 QARAALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCWPQA 481 +A L A+ ++ + E+C LY GA++DW Y+ G R + LP YPF++ W Sbjct: 584 KADLKLKAFLQGEVHELEELCRLYTIGADVDWNKFYEGTGGRTVHLPPYPFERNRYW--I 641 Query: 482 DQPPGSRTPLSSPSE 496 D G +T +P + Sbjct: 642 DNEKGEKTKAVTPEK 656 >C3HA42.1 C3HA42_BACTU Beta-ketoacyl synthase Length = 1604 Score = 469 bits (1207), Expect = e-130, Method: Compositional matrix adjust. Identities = 227/533 (42%), Positives = 351/533 (65%), Gaps = 11/533 (2%) Query: 6 LDQTDELALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMA 65 + +++ ++TG++ G+++ RI+Y L+LKG S+VLDSACSSGLL +H AC A+++ EC+MA Sbjct: 179 ITESESSSMTGNWPGLMAGRINYLLNLKGNSMVLDSACSSGLLGVHLACNALKSKECEMA 238 Query: 66 IAGGVCLIFFP-MKNPAFGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRD 124 I G+ L FFP + ++ESK + FDK ADGT + +G+ +++LKPL+KA D D Sbjct: 239 IVSGISLGFFPRFETSRKSMMESKSDKVNAFDKNADGTFFSDGVAAVILKPLNKARRDGD 298 Query: 125 PIHAVIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGD 184 I+AVIKGSA+NNDG SNGLTAPNA+AQE++ +AW NA++ PETI + EAHGTGT LGD Sbjct: 299 NIYAVIKGSAINNDGASNGLTAPNAIAQEEVLIKAWENAQVNPETIQYIEAHGTGTKLGD 358 Query: 185 PIEVQSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHF 244 PIE++ ++NAF KYT K QFCG+GSVKSNIGH A SG+AS++K +LS+KH + +++F Sbjct: 359 PIEIKGITNAFSKYTDKIQFCGVGSVKSNIGHTVAVSGLASLIKIVLSMKHNIIPPSINF 418 Query: 245 DDPNPFIDFVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAPVSPKLA 304 +PN I+FV SPV++ +K +W R +P RAGISSFGF GTN H+V+EE + Sbjct: 419 KEPNSLINFVNSPVFVTDKPHKWPRGI--VPRRAGISSFGFVGTNAHIVVEEPSIQSNDE 476 Query: 305 PASR-PYHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLALI 363 +S+ + +FT+SAK + L + + +Y + ++ L ++CYT TGRGHY YRLA+I Sbjct: 477 LSSKLTFEVFTLSAKTEKSLIQLLKKYNEFFKKGTQKSLKNICYTVNTGRGHYGYRLAII 536 Query: 364 VRDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHEQA 423 V+ +EL +K+ + ++ + +E G+Y+G KI + KK GEIT S ++ + Sbjct: 537 VKSYEELIQKIDLLCNQN-LNDISEKGVYFGYHKIGSDRKKVMDEGEITLSEHRKMNTKV 595 Query: 424 RAALGAYSGQD----IQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCWP 479 L S + + +++EIC LY++GA+++W+ Y + LP Y F++I CW Sbjct: 596 ENLLLDISNESNEYLVLNLTEICDLYIKGAKVNWKKIYVDEKRNIVSLPTYQFERIQCWG 655 Query: 480 QADQPPGSRTPL-SSPSEVFPGRKVLETYQQSVYEVELRVEEQWLLREHLMMG 531 + + TP+ + S + + ++ +++Y V++ W+L +H +MG Sbjct: 656 EV-KDRRDETPIQKNISHPLIDQCLADSMNETIYSTTFNVDKDWVLSDHKIMG 707 >B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein Length = 3695 Score = 463 bits (1191), Expect = e-128, Method: Compositional matrix adjust. Identities = 243/542 (44%), Positives = 342/542 (63%), Gaps = 31/542 (5%) Query: 2 YTHLLDQT-DELALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNN 60 Y L+D + L++TG+ I++SRISY LDLKGPS+++D+ACSS L+AIH ACQA+R Sbjct: 162 YVQLMDPSMAALSVTGNIKSIVASRISYLLDLKGPSMLVDTACSSTLVAIHLACQALRKG 221 Query: 61 ECDMAIAGGVCLIFFPM-KNPAFGL-VESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDK 118 EC+ AIAGGV LI P+ NP G+ +ES + + FD+ +DGT +GEG+ +++LKPL K Sbjct: 222 ECETAIAGGVKLILLPVDNNPQNGIGIESSSGVTRSFDEYSDGTGFGEGVAAIVLKPLKK 281 Query: 119 AVEDRDPIHAVIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGT 178 A+EDRD I+++IKGSAVN DG S G+T PNA AQ D+ +AW +A+I PETI + EAHGT Sbjct: 282 AIEDRDNIYSIIKGSAVNQDGTSMGITVPNAQAQSDVIIRAWKDAKIDPETISYIEAHGT 341 Query: 179 GTVLGDPIEVQSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKL 238 GT LGDP+E+ + AF +YT+KKQFC + SVK+NIGHL +A+GIA ++K +LSLKH++L Sbjct: 342 GTNLGDPVEIDGIEMAFAQYTNKKQFCAVSSVKTNIGHLDSAAGIAGLLKAVLSLKHKEL 401 Query: 239 VSTLHFDDPNPFIDFVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAP 298 +LHFD PN I+F SPVY+ + ++W+ P R GISSFG SGTN HLVLEE P Sbjct: 402 FPSLHFDRPNQKINFQNSPVYINDSLEQWE--NHGGPRRCGISSFGLSGTNCHLVLEEPP 459 Query: 299 VSPKLAPASRP---YHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGH 355 K P P YHIFT+S++ L E + Y +++ R ++ L LC T TGRGH Sbjct: 460 -QIKSIPEESPGCNYHIFTLSSQNINGLMEQLKNYEKYLNREREVSLGSLCSTTNTGRGH 518 Query: 356 YAYRLALIVRDIQELQEKLHRIIQEHPITGHTED----GIYYGEFKIVPESKKYKSTGEI 411 Y +RLA ++ EL+EK+ ++ TG E+ G+Y+G+ KIV + K+ + E Sbjct: 519 YNFRLAFVITSKNELKEKIQKL-----STGGLENFEKYGVYFGKHKIVDKEKESRGNNEY 573 Query: 412 TESGLNLLHEQARA----ALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRL 467 ++ + L Q A+ S Q S+ IC LYV GA + W Y+ G Y ++ L Sbjct: 574 SQEEIADLSLQTETYLNEAINNESQQKKVSLENICALYVNGANVQWNRLYKSGSYAKVSL 633 Query: 468 PVYPFQKISCWPQADQPP--GSRTPLSSPSEVFPGRKVLETYQQSVYEVELRVEEQWLLR 525 P Y F K CW + + S+ PL + +LE+ V+ +V+ QW+L Sbjct: 634 PGYSFMKKRCWIEHENSNSHNSKHPLID-------KHILESVDLDVFSTTFKVDRQWVLN 686 Query: 526 EH 527 EH Sbjct: 687 EH 688 >C3HA44.1 C3HA44_BACTU Hybrid nonribosomal peptide synthetase/polyketide synthase Length = 2216 Score = 459 bits (1180), Expect = e-127, Method: Compositional matrix adjust. Identities = 230/527 (43%), Positives = 335/527 (63%), Gaps = 31/527 (5%) Query: 2 YTHLLDQTDELALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNE 61 Y L+ D +A+TG+Y GIL+SR SY +LKGPS+V+D+ACSSGL+++H AC+A++N E Sbjct: 168 YDSLVKNADMIAMTGTYPGILASRTSYIFNLKGPSLVIDTACSSGLVSVHLACEALKNGE 227 Query: 62 CDMAIAGGVCLIFFPMKNPAFGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVE 121 C+MAIAGGV L P +E++ + FDK A GT WGEGI +++LKPL+KA+E Sbjct: 228 CNMAIAGGVNLFLRPENIGTMNEIEAENAHISIFDKNAKGTAWGEGISAIILKPLNKAIE 287 Query: 122 DRDPIHAVIKGSAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTV 181 DRD I+AVIKGSA+NNDG SNG+TAPNA AQE L ++AW NA+I+P+TI + E HGTGT+ Sbjct: 288 DRDNIYAVIKGSAINNDGASNGITAPNADAQEKLISRAWENAKISPDTISYIETHGTGTI 347 Query: 182 LGDPIEVQSLSNAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVST 241 LGDPIE++ ++NAF+KYT KKQFCGIGSVK NIGH ASG+AS++K +++LK Q L + Sbjct: 348 LGDPIEIKGITNAFKKYTDKKQFCGIGSVKPNIGHTVGASGMASLIKAVMALKEQVLPPS 407 Query: 242 LHFDDPNPFIDFVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAPVSP 301 ++F++PNP I+F SPVY+ ++ ++WK+ + P RAG+SSFGFSGTN H+VLEEAP+ Sbjct: 408 INFNEPNPLINFTTSPVYVNDRIRKWKK--NETPRRAGVSSFGFSGTNCHIVLEEAPIID 465 Query: 302 KLAPASRPYHIFTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLA 361 + + +FT+SAK K L + +Y+ + D+ L++LCYTA TGR H+ YR+ Sbjct: 466 EKIGQEQVMEVFTLSAKNKESLYNLIEKYMDFIIEETDLNLSNLCYTANTGRKHWEYRIC 525 Query: 362 LIVRDIQELQEKLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHE 421 L+V+D ++L+ K+ ++ + + + + E L + Sbjct: 526 LMVKDKEDLKRKIEKLRN------------------VSLDFQNVIKLSYMEERKLKNIAN 567 Query: 422 QARAALGAYSGQDIQSVSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCWPQA 481 Q +D+ + EIC LY +G ++DWE Y+ +I +PVY F + W Sbjct: 568 QKIEYYKQSGKKDMTVLHEICELYKRGIDIDWESLYEGEERTKISIPVYAFLRERHWVNL 627 Query: 482 DQ--PPGSRTPLSSPSEV----FPGRKVLETYQQSVYEVELRVEEQW 522 + P + ++ + V GRK YQ S E+E+ V W Sbjct: 628 HKKLPVQQKQNINIENRVEKICLNGRK---DYQYS--EIEMMVGRVW 669 >B8I979.1 B8I979_CLOCE Amino acid adenylation domain protein Length = 3739 Score = 450 bits (1157), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/469 (47%), Positives = 313/469 (66%), Gaps = 8/469 (1%) Query: 13 ALTGSYTGILSSRISYFLDLKGPSIVLDSACSSGLLAIHDACQAIRNNECDMAIAGGVCL 72 ++ G+ T +++SRISY LDLKGP++++D+ACSS L+A+H ACQA+R NEC+ AI GGV L Sbjct: 181 SVAGNVTSVIASRISYLLDLKGPAVIMDTACSSSLVALHIACQALRQNECEQAIVGGVKL 240 Query: 73 IFFPMKNPAFGLVESKLQLLKPFDKQADGTLWGEGIGSLLLKPLDKAVEDRDPIHAVIKG 132 P + ESK +K FD+ +DG +WGEG+ ++LLKPLDKA+ DRD I+AVIKG Sbjct: 241 NLLPAEGILEIGQESKNHRIKTFDEDSDGFVWGEGVAAILLKPLDKALRDRDNIYAVIKG 300 Query: 133 SAVNNDGRSNGLTAPNALAQEDLFTQAWINAEIAPETIGFYEAHGTGTVLGDPIEVQSLS 192 SA+N DG S G+ APN LAQE++ AW +A I PETI EAHGTGT +GDPIE+ ++ Sbjct: 301 SAINQDGNSVGIAAPNVLAQEEVILNAWKDANIDPETISCIEAHGTGTRIGDPIEIDAIQ 360 Query: 193 NAFRKYTSKKQFCGIGSVKSNIGHLAAASGIASVMKTILSLKHQKLVSTLHFDDPNPFID 252 AF++YT KKQFC +GSVK+ IGHL SG+A+++K IL++K++K+ + L+F PN I Sbjct: 361 RAFKRYTEKKQFCAVGSVKTAIGHLDNVSGMAALIKMILAMKNKKIPAHLNFKSPNHNIH 420 Query: 253 FVGSPVYMVNKQQEWKRAQEDIPLRAGISSFGFSGTNLHLVLEEAPVSPKLAPASRPYHI 312 F S VY+ ++ W + D P+R GI+SFG SGTN H++LEEAP + P + + Sbjct: 421 FHKSSVYVNSQLSNW---ETDGPMRCGINSFGISGTNCHVILEEAPSYERQEPIDKKLKV 477 Query: 313 FTVSAKEKTVLAEWVSRYLQHMQRAGDIELADLCYTAATGRGHYAYRLALIVRDIQELQE 372 +SAK L E V RY + + DI DLCYTA TGRGHY YRLALI D +L++ Sbjct: 478 LAISAKSSEALQELVVRYEDFLNKGYDINFDDLCYTANTGRGHYNYRLALIFYDEADLKK 537 Query: 373 KLHRIIQEHPITGHTEDGIYYGEFKIVPESKKYKSTGEITESGLNLLHEQARAALGAYSG 432 KL R I + + + GI++ K++ ES + K GE+T L + ++A L + Sbjct: 538 KL-RSINVNSLKKLNQKGIHFNIHKVINESTEPKK-GELTTKELREITKEADLVLRNFIE 595 Query: 433 QDIQS---VSEICGLYVQGAEMDWEVCYQPGGYRRIRLPVYPFQKISCW 478 ++ QS ++E+C LYV+GAE+ WE Y+ RR+RLPVYPF+K CW Sbjct: 596 KEEQSEDILNELCTLYVRGAEIKWEDLYKDENLRRMRLPVYPFEKKRCW 644 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,359,728,688 Number of extensions: 145202754 Number of successful extensions: 358686 Number of sequences better than 1.0e-05: 9641 Number of HSP's gapped: 332127 Number of HSP's successfully gapped: 10908 Length of query: 541 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 400 Effective length of database: 1,732,333,088 Effective search space: 692933235200 Effective search space used: 692933235200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)