BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0001.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase 245 2e-63 C0ZEG1.1 C0ZEG1_BREBN Putative polyketide synthase 222 3e-56 B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein 214 6e-54 B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein 209 2e-52 A5N8Z2.1 A5N8Z2_CLOK5 Predicted polyketide synthase 209 2e-52 >B8I5H4.1 B8I5H4_CLOCE Beta-ketoacyl synthase Length = 1613 Score = 245 bits (626), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 114/238 (47%), Positives = 165/238 (69%), Gaps = 1/238 (0%) Query: 5 DEIYSYILQEVGSGHLDKSIAAHLIQYLRQDQGKEADTDIAVIGVASRFSNTGSTSEVEQ 64 + ++ YI++ G+G++ K AA +I+ L+ D+ + +D DIA++G+A++ + E Sbjct: 2 NRVFQYIVENTGNGNISKKAAAQIIRLLKDDEIR-SDNDIAIVGMAAKLPFAPNIDEFWI 60 Query: 65 LLTLGVDSIQELPAGRKEQADLFLPLFQVEKDHAQYDKAGYLSEIDPFDFAFFNISPREA 124 + G D + + P RK+ D L ++ +Y +L E+D FD+ FF +SP+EA Sbjct: 61 NIRNGRDCVSDFPEERKKDTDALLGHMNLKPSEIEYSIGAFLDEVDKFDYKFFKLSPKEA 120 Query: 125 SLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGVFIGYSSDFGEEYKKGIQMLVPEAMG 184 SL+DPNQRLFLQ A++AIE++GYG +L+GSKTGV++GYSSDFGE YK+ I + P G Sbjct: 121 SLIDPNQRLFLQTAYQAIEDSGYGNGRLSGSKTGVYVGYSSDFGESYKRFIHTVDPGLSG 180 Query: 185 VAIPGNIKSVIASRISHYLDLKGPAVVFDTACSSSLVAIHFACRQLIEGSCDLXXXGG 242 +AIPGNIKS+IASRIS+YLDLKGP+++ DTACSSSLVAIH ACR + G C+ GG Sbjct: 181 IAIPGNIKSIIASRISYYLDLKGPSMLVDTACSSSLVAIHMACRAIRNGECETALAGG 238 >C0ZEG1.1 C0ZEG1_BREBN Putative polyketide synthase Length = 1122 Score = 222 bits (565), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 107/203 (52%), Positives = 146/203 (71%), Gaps = 6/203 (2%) Query: 43 DIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVE---KDHAQ 99 DIAVIG A RF + + + +LL G D ++ELP KE+ + +PL + + Q Sbjct: 30 DIAVIGAACRFGSAENPEDFWRLLQAGQDCVRELP---KERQNDLIPLLLAKGLTMEQIQ 86 Query: 100 YDKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGV 159 + +A YL+EID FD F +SP+EAS MDPNQRLFL WEAIE+AGYGGT L GS+TGV Sbjct: 87 FREAAYLNEIDKFDPQLFGLSPKEASQMDPNQRLFLLTCWEAIEDAGYGGTMLAGSRTGV 146 Query: 160 FIGYSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVVFDTACSSS 219 ++G+SSDFGEEY++ I+ L PEA+ +I GN+KSVIASR+S+ LDLKGP+++ DT+CSSS Sbjct: 147 YLGFSSDFGEEYRRMIRELEPEALSTSIIGNMKSVIASRVSYLLDLKGPSMLVDTSCSSS 206 Query: 220 LVAIHFACRQLIEGSCDLXXXGG 242 L+A+H AC+ + G C++ GG Sbjct: 207 LMAVHLACQAIRRGECEMALAGG 229 >B8I5H5.1 B8I5H5_CLOCE Amino acid adenylation domain protein Length = 3695 Score = 214 bits (545), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 96/200 (48%), Positives = 139/200 (69%) Query: 43 DIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLFLPLFQVEKDHAQYDK 102 D+A+IG++ +F++ G+ E L G + + + P RK+ D L + + Y + Sbjct: 31 DVAIIGISGKFASCGNMDEFYVSLRNGDNLVGKFPQRRKQDIDTILRYMGISEKETVYYE 90 Query: 103 AGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAGYGGTQLTGSKTGVFIG 162 +L+EID FD + FNISP+EA LMDPNQRLFLQ AW AIE++GYGG +L S+TGV++G Sbjct: 91 GAFLNEIDKFDCSLFNISPKEAGLMDPNQRLFLQTAWNAIEDSGYGGDRLRSSRTGVYVG 150 Query: 163 YSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLKGPAVVFDTACSSSLVA 222 Y DF E+Y+K +Q++ P +++ GNIKS++ASRIS+ LDLKGP+++ DTACSS+LVA Sbjct: 151 YCGDFNEDYRKYVQLMDPSMAALSVTGNIKSIVASRISYLLDLKGPSMLVDTACSSTLVA 210 Query: 223 IHFACRQLIEGSCDLXXXGG 242 IH AC+ L +G C+ GG Sbjct: 211 IHLACQALRKGECETAIAGG 230 >B9E2D1.1 B9E2D1_CLOK1 Putative uncharacterized protein Length = 1009 Score = 209 bits (532), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 98/216 (45%), Positives = 147/216 (68%), Gaps = 6/216 (2%) Query: 33 RQDQGKEADT---DIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLF-- 87 + D K DT DIA+IG++ RF+ + E +L G D I++ P R + + Sbjct: 18 KDDSIKFQDTSRHDIAIIGISGRFAEAENLKEYWNILKNGRDCIRKFPEDRASEITNYVE 77 Query: 88 -LPLFQVEKDHAQYDKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAG 146 L + K+ ++ +AG+L+ +D FD+ FF +SPREASLMDPNQR+FL+ W AIE+AG Sbjct: 78 QLYSLGLSKEGMKFIEAGFLNHVDQFDYNFFGLSPREASLMDPNQRIFLETVWSAIEDAG 137 Query: 147 YGGTQLTGSKTGVFIGYSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLK 206 Y ++ G+KTGV++G+S+DF ++Y+K +Q + E++G++ GNIKS+IASRIS+ LD K Sbjct: 138 YNSKKIRGTKTGVYLGFSNDFHDDYRKYVQTMSSESIGISTAGNIKSIIASRISYLLDFK 197 Query: 207 GPAVVFDTACSSSLVAIHFACRQLIEGSCDLXXXGG 242 GP++V DTACSS+LVA+H AC+ + +G CD GG Sbjct: 198 GPSLVVDTACSSALVAVHLACQSIRKGECDAAIAGG 233 >A5N8Z2.1 A5N8Z2_CLOK5 Predicted polyketide synthase Length = 1009 Score = 209 bits (532), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 98/216 (45%), Positives = 147/216 (68%), Gaps = 6/216 (2%) Query: 33 RQDQGKEADT---DIAVIGVASRFSNTGSTSEVEQLLTLGVDSIQELPAGRKEQADLF-- 87 + D K DT DIA+IG++ RF+ + E +L G D I++ P R + + Sbjct: 18 KDDSIKFQDTSRHDIAIIGISGRFAEAENLKEYWNILKNGRDCIRKFPEDRASEITNYVE 77 Query: 88 -LPLFQVEKDHAQYDKAGYLSEIDPFDFAFFNISPREASLMDPNQRLFLQLAWEAIEEAG 146 L + K+ ++ +AG+L+ +D FD+ FF +SPREASLMDPNQR+FL+ W AIE+AG Sbjct: 78 QLYSLGLSKEGMKFIEAGFLNHVDQFDYNFFGLSPREASLMDPNQRIFLETVWSAIEDAG 137 Query: 147 YGGTQLTGSKTGVFIGYSSDFGEEYKKGIQMLVPEAMGVAIPGNIKSVIASRISHYLDLK 206 Y ++ G+KTGV++G+S+DF ++Y+K +Q + E++G++ GNIKS+IASRIS+ LD K Sbjct: 138 YNSKKIRGTKTGVYLGFSNDFHDDYRKYVQTMSSESIGISTAGNIKSIIASRISYLLDFK 197 Query: 207 GPAVVFDTACSSSLVAIHFACRQLIEGSCDLXXXGG 242 GP++V DTACSS+LVA+H AC+ + +G CD GG Sbjct: 198 GPSLVVDTACSSALVAVHLACQSIRKGECDAAIAGG 233 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,567,306,810 Number of extensions: 63732602 Number of successful extensions: 156409 Number of sequences better than 1.0e-05: 5050 Number of HSP's gapped: 148848 Number of HSP's successfully gapped: 6244 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)