BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0488.0003.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9VRW8.1 A9VRW8_BACWK Amidase 332 2e-89 C3A532.1 C3A532_BACMY Glutaminyl-tRNA synthase, glutamine-hydrol... 330 1e-88 C2SJ58.1 C2SJ58_BACCE Glutaminyl-tRNA synthase, glutamine-hydrol... 330 1e-88 C2PV47.1 C2PV47_BACCE Glutaminyl-tRNA synthase, glutamine-hydrol... 329 2e-88 C2XT57.1 C2XT57_BACCE Glutaminyl-tRNA synthase, glutamine-hydrol... 328 4e-88 >A9VRW8.1 A9VRW8_BACWK Amidase Length = 491 Score = 332 bits (852), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 158/285 (55%), Positives = 209/285 (73%), Gaps = 1/285 (0%) Query: 2 SVAGIKPTVGLISRTGIIPFSYSQDTAGPLARTVSDAAILLGVLAGRDESDPATWRYAGP 61 SV GIKPTVGLISR+GIIPF+YSQDTAGP ARTV+DAAILLG L G DE D AT + G Sbjct: 205 SVVGIKPTVGLISRSGIIPFTYSQDTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGM 264 Query: 62 DGGQDYTGYLNVEGLRGARIGIYCDVPEHVLSSGEIDEFLFDQAVSDLTKAGAQVVKPIE 121 QDYT YL+ GL+GA+IG+Y + P+ +GE DE LF++ + L GA VV+ I+ Sbjct: 265 -AYQDYTSYLDANGLKGAKIGVYSNAPKDYYENGEYDEKLFEETIQVLRSEGATVVEDID 323 Query: 122 IPSFHGPWKYNKINMEFKHGIQNFLHNLPAHMPIHSLSELIHWNQNHKEQALRYGQDILE 181 IPSFH W + E KH + N+L LP+ +P+HS+SEL+ +N+N E+AL+YGQ LE Sbjct: 324 IPSFHREWSWGVPLYELKHSLDNYLSKLPSTIPVHSISELMEFNKNIAERALKYGQTKLE 383 Query: 182 YRESLTDLLNDRKYIQESVLDLHLAQREGIDHAIDHYQLDAIMFPAYIGADLSARAGYPS 241 R+ + L + +Y+ + D++ +Q++GID A++ Y LDAI+FP+YIG+ + A+AGYPS Sbjct: 384 RRKDFPNTLRNPEYLNARLEDIYFSQKQGIDFALEKYNLDAILFPSYIGSTICAKAGYPS 443 Query: 242 IAVPAGYMENGRPFGVTFADKAFSEPALIAIAYSFEQHTKHRKQP 286 IA+PAGYM+NGRPFG+T A AFSE ALI +AY+FEQ TKHRK P Sbjct: 444 IAMPAGYMDNGRPFGITLASAAFSEGALIKLAYAFEQATKHRKMP 488 >C3A532.1 C3A532_BACMY Glutaminyl-tRNA synthase, glutamine-hydrolyzing, subunit A (Glutamyl-tRNA(Gln) amidotransferase, subunit A) Length = 493 Score = 330 bits (846), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 159/285 (55%), Positives = 210/285 (73%), Gaps = 1/285 (0%) Query: 2 SVAGIKPTVGLISRTGIIPFSYSQDTAGPLARTVSDAAILLGVLAGRDESDPATWRYAGP 61 SV GIKPTVGLISR+GIIPF+YSQDTAGP ARTV+DAAILLG L G DE D AT + G Sbjct: 207 SVVGIKPTVGLISRSGIIPFTYSQDTAGPFARTVTDAAILLGNLTGVDEMDVATHKSEGM 266 Query: 62 DGGQDYTGYLNVEGLRGARIGIYCDVPEHVLSSGEIDEFLFDQAVSDLTKAGAQVVKPIE 121 QDYT YL+V GL+GA+IG++ + P+ +GE DE LF++ + L GA VV+ I+ Sbjct: 267 -AYQDYTPYLDVNGLKGAKIGVFNNAPKDYYENGEYDEKLFEETIQVLRSEGATVVEDID 325 Query: 122 IPSFHGPWKYNKINMEFKHGIQNFLHNLPAHMPIHSLSELIHWNQNHKEQALRYGQDILE 181 IPSFH W + E KH + N+L LP+ +P+HS+SEL+ +N+N E+ALRYGQ LE Sbjct: 326 IPSFHREWSWGVPLYELKHSLDNYLSKLPSTIPVHSISELMEFNKNIAERALRYGQTKLE 385 Query: 182 YRESLTDLLNDRKYIQESVLDLHLAQREGIDHAIDHYQLDAIMFPAYIGADLSARAGYPS 241 R+ + L + +Y+ + D++ +Q++GID A++ Y LDAI+FP+YIG+ + A+AGYPS Sbjct: 386 RRKDFPNTLRNPEYLNARLEDIYFSQKQGIDFALEKYNLDAILFPSYIGSTICAKAGYPS 445 Query: 242 IAVPAGYMENGRPFGVTFADKAFSEPALIAIAYSFEQHTKHRKQP 286 IA+PAGYM+NGRPFG+T A AFSE ALI +AY+FEQ TKHRK P Sbjct: 446 IAMPAGYMDNGRPFGITLASAAFSEGALIKLAYAFEQATKHRKIP 490 >C2SJ58.1 C2SJ58_BACCE Glutaminyl-tRNA synthase, glutamine-hydrolyzing, subunit A (Glutamyl-tRNA(Gln) amidotransferase, subunit A) Length = 493 Score = 330 bits (845), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 158/285 (55%), Positives = 208/285 (72%), Gaps = 1/285 (0%) Query: 2 SVAGIKPTVGLISRTGIIPFSYSQDTAGPLARTVSDAAILLGVLAGRDESDPATWRYAGP 61 SV GIKPTVGLISR GIIPF+YSQDTAGP ARTV+DAAILLG L G DE D AT + G Sbjct: 207 SVVGIKPTVGLISRRGIIPFTYSQDTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGM 266 Query: 62 DGGQDYTGYLNVEGLRGARIGIYCDVPEHVLSSGEIDEFLFDQAVSDLTKAGAQVVKPIE 121 QDYT YL+ GL+GA+IG+Y + P+ +GE DE LF++ + L GA VV+ I+ Sbjct: 267 -AYQDYTSYLDANGLKGAKIGVYSNAPKDYYENGEYDEKLFEETIQVLRSEGATVVEDID 325 Query: 122 IPSFHGPWKYNKINMEFKHGIQNFLHNLPAHMPIHSLSELIHWNQNHKEQALRYGQDILE 181 IPSFH W + E KH + N+L LP+ +P+HS+SEL+ +N+N E+AL+YGQ LE Sbjct: 326 IPSFHREWSWGVPLYELKHSLDNYLSKLPSTIPVHSISELMEFNKNIAERALKYGQTKLE 385 Query: 182 YRESLTDLLNDRKYIQESVLDLHLAQREGIDHAIDHYQLDAIMFPAYIGADLSARAGYPS 241 R+ + L + +Y+ + D++ +Q++GID A++ Y LDAI+FP+YIG+ + A+AGYPS Sbjct: 386 RRKDFPNTLRNPEYLNARLEDIYFSQKQGIDFALEKYNLDAILFPSYIGSTICAKAGYPS 445 Query: 242 IAVPAGYMENGRPFGVTFADKAFSEPALIAIAYSFEQHTKHRKQP 286 IA+PAGYM+NGRPFG+T A AFSE ALI +AY+FEQ TKHRK P Sbjct: 446 IAMPAGYMDNGRPFGITLASAAFSEGALIKLAYAFEQATKHRKIP 490 >C2PV47.1 C2PV47_BACCE Glutaminyl-tRNA synthase, glutamine-hydrolyzing, subunit A (Glutamyl-tRNA(Gln) amidotransferase, subunit A) Length = 493 Score = 329 bits (844), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 157/285 (55%), Positives = 208/285 (72%), Gaps = 1/285 (0%) Query: 2 SVAGIKPTVGLISRTGIIPFSYSQDTAGPLARTVSDAAILLGVLAGRDESDPATWRYAGP 61 SV GIKPTVGLISR+GIIPF+YSQDTAGP ARTV+DAAILLG L G DE D AT + G Sbjct: 207 SVVGIKPTVGLISRSGIIPFTYSQDTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGM 266 Query: 62 DGGQDYTGYLNVEGLRGARIGIYCDVPEHVLSSGEIDEFLFDQAVSDLTKAGAQVVKPIE 121 QDYT YL+ GL+GA+IG++ + P+ SGE DE LF + + L GA VV+ I+ Sbjct: 267 -AYQDYTSYLDANGLKGAKIGVFSNAPKDYYESGEYDEKLFKETIQVLRSKGATVVEDID 325 Query: 122 IPSFHGPWKYNKINMEFKHGIQNFLHNLPAHMPIHSLSELIHWNQNHKEQALRYGQDILE 181 IPSFH W + E KH + N+L LP+ +P+HS+SEL+ +N+N E+AL+YGQ LE Sbjct: 326 IPSFHREWSWGVPLYELKHSLDNYLSKLPSTIPVHSISELMEFNKNIAERALKYGQTKLE 385 Query: 182 YRESLTDLLNDRKYIQESVLDLHLAQREGIDHAIDHYQLDAIMFPAYIGADLSARAGYPS 241 ++ + L + +Y++ + D++ +Q +GID A++ Y LDAI+FP+YIG+ + A+AGYPS Sbjct: 386 RKKDFPNTLRNPEYLKARLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPS 445 Query: 242 IAVPAGYMENGRPFGVTFADKAFSEPALIAIAYSFEQHTKHRKQP 286 IA+PAGYM+NGRPFG+T A AFSE ALI +AY+FEQ TKHRK P Sbjct: 446 IAMPAGYMDNGRPFGITLASAAFSEGALIKLAYAFEQATKHRKIP 490 >C2XT57.1 C2XT57_BACCE Glutaminyl-tRNA synthase, glutamine-hydrolyzing, subunit A (Glutamyl-tRNA(Gln) amidotransferase, subunit A) Length = 493 Score = 328 bits (841), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 157/285 (55%), Positives = 209/285 (73%), Gaps = 1/285 (0%) Query: 2 SVAGIKPTVGLISRTGIIPFSYSQDTAGPLARTVSDAAILLGVLAGRDESDPATWRYAGP 61 SV GIKPTVGLISR+GIIPF+YSQDTAGP ARTV+DAAILLG L G DE D AT + G Sbjct: 207 SVVGIKPTVGLISRSGIIPFTYSQDTAGPFARTVTDAAILLGSLTGVDEKDVATHKSEGM 266 Query: 62 DGGQDYTGYLNVEGLRGARIGIYCDVPEHVLSSGEIDEFLFDQAVSDLTKAGAQVVKPIE 121 QDYT YL+ GL+GA+IG+Y + P+ +GE DE LF++ + L GA VV+ I+ Sbjct: 267 -AYQDYTPYLDANGLKGAKIGVYSNAPKDYYENGEYDEKLFEETIQVLRSEGATVVEDID 325 Query: 122 IPSFHGPWKYNKINMEFKHGIQNFLHNLPAHMPIHSLSELIHWNQNHKEQALRYGQDILE 181 IPSFH W + E KH + N+L LP+ +P+HS+SEL+ +N+N E+AL+YGQ LE Sbjct: 326 IPSFHREWSWGVPLYELKHSLDNYLSKLPSTIPVHSISELMEFNKNIAERALKYGQTKLE 385 Query: 182 YRESLTDLLNDRKYIQESVLDLHLAQREGIDHAIDHYQLDAIMFPAYIGADLSARAGYPS 241 ++ + L + +Y++ + D++ +Q +GID A++ Y LDAI+FP+YIG+ + A+AGYPS Sbjct: 386 RKKDFPNTLRNPEYLKARLEDIYFSQEQGIDFALEKYNLDAILFPSYIGSTICAKAGYPS 445 Query: 242 IAVPAGYMENGRPFGVTFADKAFSEPALIAIAYSFEQHTKHRKQP 286 IA+PAGYM+NGRPFG+T A AFSE ALI +AY+FEQ TKHRK P Sbjct: 446 IAMPAGYMDNGRPFGITLASAAFSEGALIKLAYAFEQATKHRKIP 490 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,845,233,870 Number of extensions: 82115255 Number of successful extensions: 193409 Number of sequences better than 1.0e-05: 1587 Number of HSP's gapped: 192426 Number of HSP's successfully gapped: 1592 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)