BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0488.0001.p (390 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KHD5.1 B3KHD5_9BACI Threonyl/alanyl tRNA synthetase SAD 493 e-137 C3IZX3.1 C3IZX3_9BACI Threonyl/alanyl tRNA synthetase SAD 303 2e-80 B7YCY5.1 B7YCY5_9BACI Threonyl/alanyl tRNA synthetase SAD 303 2e-80 Q0W0A4.1 Q0W0A4_UNCMA Alanyl-tRNS synthetase-related protein 271 1e-70 Q65H19.1 Q65H19_BACLD Putative uncharacterized protein 265 7e-69 >B3KHD5.1 B3KHD5_9BACI Threonyl/alanyl tRNA synthetase SAD Length = 394 Score = 493 bits (1270), Expect = e-137, Method: Compositional matrix adjust. Identities = 233/390 (59%), Positives = 300/390 (76%), Gaps = 2/390 (0%) Query: 1 MTKKLYYESAYLKDWHTSVSQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLDV 60 MTKKLYYESAY ++W T +++ +EREDG YL+LEETAFYPHGGGQPCD G I IPVLDV Sbjct: 1 MTKKLYYESAYTREWETRITRKLEREDGTYLVLEETAFYPHGGGQPCDLGWIDKIPVLDV 60 Query: 61 ILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHLG 120 + E+ +LHK+E LP +V+C+LDW+RRFDHMQ HSGQHLLSA+ ++ +A TLSFHLG Sbjct: 61 VQEDGEILHKIERLPEREEVTCRLDWDRRFDHMQHHSGQHLLSAMCLQVCEAETLSFHLG 120 Query: 121 NDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTENI 180 DYATID+ +L QL E EVN Q+ L+R + +Y V+ E+ A+LPLVK PKVT++I Sbjct: 121 QDYATIDIARSDLPQHQLMMLELEVNDQIYLNRQVTNYVVSREQAAALPLVKQPKVTDDI 180 Query: 181 RIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRIL 240 RIVEIEG+E+NACGGTHVS+TG IGMIKLLRAEKQKG++R+ FKCG RAL E N IL Sbjct: 181 RIVEIEGIEYNACGGTHVSATGEIGMIKLLRAEKQKGHMRLYFKCGYRALKEFNAGMEIL 240 Query: 241 SALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRVVAH 300 +LSA+FNT K+EILDRI KW+ EQKQLQAE+ ++ +ND Y+A +LL+ E + R+V H Sbjct: 241 GSLSARFNTAKEEILDRIAKWDQEQKQLQAELNAVKAENDAYLAGKLLA--ERQGRLVKH 298 Query: 301 IFADKSLKDLQSLATKLTANTELPVLLLTAMENKAVLACSGSAGLSCGAFFKAHLGEFQG 360 +FADK +KD+Q+LA KLT E+ VL + ++K VLA G +G +CGAF K HL +++G Sbjct: 299 VFADKPMKDMQNLAAKLTEQAEVIVLFASNSDHKVVLAQGGESGPACGAFLKQHLADYRG 358 Query: 361 KGGGSDKMAQAGFPAWDEVLKFYEFAKVNL 390 KGGGSDK+AQAGF ++ + FY F +L Sbjct: 359 KGGGSDKIAQAGFSTVEDAMAFYRFTAQSL 388 >C3IZX3.1 C3IZX3_9BACI Threonyl/alanyl tRNA synthetase SAD Length = 389 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 164/384 (42%), Positives = 230/384 (59%), Gaps = 7/384 (1%) Query: 1 MTKKLYYESAYLKDWHTSVSQVVEREDG-LYLI-LEETAFYPHGGGQPCDAGTIGGIPVL 58 MT+KLYY + + W T+V+ V R+DG Y++ L ETAFYP GGGQP DAGTI G+PVL Sbjct: 1 MTEKLYYTAPDVAAWTTAVTSV--RQDGETYVVTLAETAFYPEGGGQPSDAGTIAGLPVL 58 Query: 59 DVILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFH 118 DV E V H++ + P VSC LD RR DH QQHSGQHLLSAVF E A T+ FH Sbjct: 59 DVFDENGEVYHRLPAHPGMGVVSCVLDMKRRRDHTQQHSGQHLLSAVFIEQLGAETVGFH 118 Query: 119 LGNDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTE 178 LG D TID+ + LT ++ A E N + + + +Y+VTPEE +P K P V Sbjct: 119 LGRDVVTIDITVDALTESEMLAVENRANELIYANLPVDTYWVTPEEAERMPFRKRPDVQG 178 Query: 179 NIRIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQR 238 N+R+VE++GV+ +AC GTHV TG IG+IKLL+AE+ +G R++F CGGRAL + Sbjct: 179 NVRVVEMKGVDLSACCGTHVKRTGEIGVIKLLKAERHRGMTRLSFLCGGRALADYQQTHH 238 Query: 239 ILSALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRVV 298 I+ A + +F T +D++L+ I KWE E+K+L+ LR+ +I A E R V Sbjct: 239 IVQAAAGRFGTSRDQLLEVIVKWEQEKKELEKR---LRQYEEIVFAVEAKRAAAESGRAV 295 Query: 299 AHIFADKSLKDLQSLATKLTANTELPVLLLTAMENKAVLACSGSAGLSCGAFFKAHLGEF 358 + K+LQ +A + LL + + +AV+A + S+ L G + K F Sbjct: 296 IGRYDQYGAKELQIIARLIANRYGKTALLASTPDWRAVIAKAPSSLLHAGNWLKEQAALF 355 Query: 359 QGKGGGSDKMAQAGFPAWDEVLKF 382 GKGGG+++ QA FP+ + + +F Sbjct: 356 GGKGGGNERQGQAAFPSLEALSQF 379 >B7YCY5.1 B7YCY5_9BACI Threonyl/alanyl tRNA synthetase SAD Length = 389 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 164/384 (42%), Positives = 230/384 (59%), Gaps = 7/384 (1%) Query: 1 MTKKLYYESAYLKDWHTSVSQVVEREDG-LYLI-LEETAFYPHGGGQPCDAGTIGGIPVL 58 MT+KLYY + + W T+V+ V R+DG Y++ L ETAFYP GGGQP DAGTI G+PVL Sbjct: 1 MTEKLYYTAPDVAAWTTAVTSV--RQDGETYVVTLAETAFYPEGGGQPSDAGTIAGLPVL 58 Query: 59 DVILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFH 118 DV E V H++ + P VSC LD RR DH QQHSGQHLLSAVF E A T+ FH Sbjct: 59 DVFDENGEVYHRLPAHPGMGVVSCVLDMKRRRDHTQQHSGQHLLSAVFIEQLGAETVGFH 118 Query: 119 LGNDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTE 178 LG D TID+ + LT ++ A E N + + + +Y+VTPEE +P K P V Sbjct: 119 LGRDVVTIDITVDALTESEMLAVENRANELIYANLPVDTYWVTPEEAERMPFRKRPDVQG 178 Query: 179 NIRIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQR 238 N+R+VE++GV+ +AC GTHV TG IG+IKLL+AE+ +G R++F CGGRAL + Sbjct: 179 NVRVVEMKGVDLSACCGTHVKRTGEIGVIKLLKAERHRGMTRLSFLCGGRALADYQQTHH 238 Query: 239 ILSALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRVV 298 I+ A + +F T +D++L+ I KWE E+K+L+ LR+ +I A E R V Sbjct: 239 IVQAAAGRFGTSRDQLLEVIVKWEQEKKELEKR---LRQYEEIVFAVEAKRAAAESGRAV 295 Query: 299 AHIFADKSLKDLQSLATKLTANTELPVLLLTAMENKAVLACSGSAGLSCGAFFKAHLGEF 358 + K+LQ +A + LL + + +AV+A + S+ L G + K F Sbjct: 296 IGRYDQYGAKELQIIARLIANRYGKTALLASTPDWRAVIAKAPSSLLHAGNWLKEQAALF 355 Query: 359 QGKGGGSDKMAQAGFPAWDEVLKF 382 GKGGG+++ QA FP+ + + +F Sbjct: 356 GGKGGGNERQGQAAFPSLEALSQF 379 >Q0W0A4.1 Q0W0A4_UNCMA Alanyl-tRNS synthetase-related protein Length = 390 Score = 271 bits (692), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 153/386 (39%), Positives = 210/386 (54%), Gaps = 4/386 (1%) Query: 1 MTKKLYYESAYLKDWHTSVSQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLDV 60 M + LYYES +W T V V+ ++ +++L ET FYP GGGQP D GTI GIPV V Sbjct: 1 MARLLYYESPRTTEWQTEVVNVLNKDGKYHVVLAETNFYPEGGGQPADRGTIAGIPVEHV 60 Query: 61 ILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHLG 120 E V H + PA V+C+LDW RRFD MQQHSGQHLLSAV T S H+ Sbjct: 61 YEEGGEVYHVLAFAPAEKTVACKLDWARRFDLMQQHSGQHLLSAVMYNNHGWETGSLHMS 120 Query: 121 NDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTENI 180 + ++D+ ++TP+ L E N V D I ++ VTPEE P K+P I Sbjct: 121 EEGLSVDVLTPDVTPEALKEIEDAANEVVTRDLEIKTHIVTPEEAKKYPNRKIPPTAGTI 180 Query: 181 RIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRIL 240 RIVEI + + C GTHV+ TG IG+IK+ +K K ++R+ FKCGGRAL + Q I+ Sbjct: 181 RIVEISSHDFSPCCGTHVARTGEIGIIKITGTQKMKDSMRLFFKCGGRALRDYQAKQEII 240 Query: 241 SALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELL-SGLEPETRVVA 299 + LS F + ++L RI EAE + E+ L+++ A L S P RV Sbjct: 241 TKLSQLFKADQGKLLSRIEAHEAEARAHWKELTELKDKLLKIEAGNLARSASTPVIRVA- 299 Query: 300 HIFADKSLKDLQSLATKLTANTELPVLLLTAMENKAVLACSGSAGLSCGAFFKAHLGEFQ 359 + DKS DL SL+ L + ++L + E + + A S + CG K HL F Sbjct: 300 --YEDKSFNDLMSLSKYLLQAGDFIMILASVPEKRLLFARSKKFDVDCGKLMKEHLAAFN 357 Query: 360 GKGGGSDKMAQAGFPAWDEVLKFYEF 385 GKGGG + A AGF + +++ +F F Sbjct: 358 GKGGGKPEFANAGFSSVEDLQRFEAF 383 >Q65H19.1 Q65H19_BACLD Putative uncharacterized protein Length = 410 Score = 265 bits (677), Expect = 7e-69, Method: Compositional matrix adjust. Identities = 153/381 (40%), Positives = 227/381 (59%), Gaps = 10/381 (2%) Query: 4 KLYYESAYLKDWHTSV-SQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLDVIL 62 KL+Y+ YLK + T + Q +++ +Y+ILEETAFYP GGGQP D GT+ G+ VL+V Sbjct: 7 KLFYKDPYLKTFETKILKQQQDKKGDVYIILEETAFYPTGGGQPHDTGTLNGVKVLNVDE 66 Query: 63 EENVVLHKVES-LPA-GSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHLG 120 + + H VE LP QV +DW RRFDHMQQH+GQH+LSA F +L+ T+ FHLG Sbjct: 67 IDGEIRHYVERPLPHLNDQVVGVIDWERRFDHMQQHTGQHILSAAFAQLYDLHTVGFHLG 126 Query: 121 NDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTENI 180 ND TIDL++ ELT + AE+ N + +R+IV+ +V +++ + P+ KLP V ENI Sbjct: 127 NDTVTIDLDVSELTEEMAGKAEKLANEIITENRSIVAKWVEYDDLVNYPIRKLPSVKENI 186 Query: 181 RIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRIL 240 R+V I+ ++N CGGTH S+TG +G IK+L E+Q+ IR+ F CG R + + + Q+ + Sbjct: 187 RLVMIDEFDYNGCGGTHPSATGEVGAIKVLNWERQRKKIRLYFVCGNRVITQFDQKQKAI 246 Query: 241 SALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELL--SGLEPETRVV 298 L+ N + E+ + I + K+L + E+N Y A ELL S ++ +T +V Sbjct: 247 VELTGLLNRPEGELSEAIKRLLENVKKLDKALLESYEKNLQYEANELLAKSAIQNDTVMV 306 Query: 299 AHIFADKSLKDLQSLATKLTANTELPVLLLTAMEN--KAVLACS-GSAG-LSCGAFFKAH 354 F+D+S+++LQ LA K+ A+ E L+ EN + L C G A ++ + Sbjct: 307 IETFSDRSIQELQKLA-KMIADREKRALIFFLSENDHRFQLVCGRGEANKMNMRDLMQEA 365 Query: 355 LGEFQGKGGGSDKMAQAGFPA 375 L GKGGG+ AQ G A Sbjct: 366 LSLIDGKGGGNKMFAQGGGTA 386 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,263,724,472 Number of extensions: 93237218 Number of successful extensions: 257786 Number of sequences better than 1.0e-05: 2133 Number of HSP's gapped: 253087 Number of HSP's successfully gapped: 2153 Length of query: 390 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 252 Effective length of database: 1,760,596,133 Effective search space: 443670225516 Effective search space used: 443670225516 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)