BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0486.0010.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z4C9.1 C0Z4C9_BREBN Putative ABC transporter substrate binding... 222 3e-56 B1D4P1.1 B1D4P1_9BACL Periplasmic binding protein/LacI transcrip... 192 2e-47 B3KJF9.1 B3KJF9_9BACI Periplasmic binding protein/LacI transcrip... 188 5e-46 B1DIU2.1 B1DIU2_9BACL Periplasmic binding protein/LacI transcrip... 183 2e-44 C0C8B2.1 C0C8B2_9FIRM Putative uncharacterized protein 170 2e-40 >C0Z4C9.1 C0Z4C9_BREBN Putative ABC transporter substrate binding protein Length = 328 Score = 222 bits (566), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 119/299 (39%), Positives = 179/299 (59%), Gaps = 15/299 (5%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIIL 60 +IV+M GDYW TVKLGAE AAKE+ L F APD E D Q+ +V+++I ++ I++ Sbjct: 43 LIVRMKHGDYWRTVKLGAEMAAKEYERNLNFYAPDYEEDAQRQMELVQQAIADGSETIVV 102 Query: 61 AASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGY 120 A S L + + IP++ +D + VK+Y+G++ Y+ G+K+ E+++ L+ Sbjct: 103 APSDERVLREAIKLTRERAIPILVLDTVGNDSAVKSYIGTDNYDMGRKAFEKMVYLIEKK 162 Query: 121 GEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTAD 180 G+I ++ GT A +A RE+G L+ R VE+V S+ Sbjct: 163 GQIVLL-------------GTDRIKA-NAKRREQGVLDLLPREMQVELVANENAPSDKKQ 208 Query: 181 AEQLTRDMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSMLEMLQDG 239 + TR+++R HP+L+G+ L+ + + G + ++ G +K+VA DS P +LE LQ+G Sbjct: 209 IGEWTRELIRKHPQLKGVIALDSSTAIGVAEELESSGLRDKVKIVAIDSPPEVLEYLQEG 268 Query: 240 TVQATVIQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNMLWPENQKLLFPFV 298 + AT+IQ P + GYL VKYAVEA+ G VP VDTGTK+ID +NM W ENQKLLFPFV Sbjct: 269 IISATIIQKPLSMGYLGVKYAVEASNGEAVPSLVDTGTKVIDRENMFWSENQKLLFPFV 327 >B1D4P1.1 B1D4P1_9BACL Periplasmic binding protein/LacI transcriptional regulator Length = 332 Score = 192 bits (489), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 103/293 (35%), Positives = 170/293 (58%), Gaps = 17/293 (5%) Query: 9 DYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIILAASSYMGL 68 D+W + G E AA EF +T P SE++ID Q ++++I+QK D I++AA+ Y L Sbjct: 52 DFWKVLITGVEDAANEFGANVTVIGPPSETEIDTQTEQLKQAIRQKPDAIVMAATDYNRL 111 Query: 69 AQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLL--NGYGEIGII 126 V + I +++VD+ + S +++ ++ AG+K+ E + LL + ++ I+ Sbjct: 112 VPVAQQIVKAGIKLITVDSRINSDDALSFIATDNIAAGEKAGEEMAKLLPESSSSKVAIM 171 Query: 127 NFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTADAEQLTR 186 ++ TS Q EREKG + ++EV Y+ A +LT+ Sbjct: 172 SYVYGTSSQL--------------EREKGVRQWLEGHSHIEVASTLYSEGRLEKAYELTK 217 Query: 187 DMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSMLEMLQDGTVQATV 245 ++L P ++GI LNE ++ GAG+ I++LG G +K+V FDSS +++++ G +QAT+ Sbjct: 218 ELLSEDPGIKGIVGLNEPSTVGAGRAIRDLGLSGKVKLVGFDSSVDEVKLIEGGVLQATI 277 Query: 246 IQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNMLWPENQKLLFPFV 298 +Q P+N GYL+VK A+EA G VP+ +DT + +I+ DNM +NQKLLFPFV Sbjct: 278 VQKPYNMGYLSVKTAIEAVRGKKVPKLIDTESVIINKDNMYTEQNQKLLFPFV 330 >B3KJF9.1 B3KJF9_9BACI Periplasmic binding protein/LacI transcriptional regulator Length = 326 Score = 188 bits (478), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 103/295 (34%), Positives = 164/295 (55%), Gaps = 15/295 (5%) Query: 5 MNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIILAASS 64 ++ ++W + G A++EF V ++ P E ++D QI ++EE++KQK D I+LAA Sbjct: 44 LDENEFWRVLIEGVNTASREFGVDVSVVGPSQEIEVDLQIQLLEEAVKQKPDAIVLAAGD 103 Query: 65 YMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGYGEIG 124 Y L V + IP++ +D+ V + + ++ AGQK+ + L L + ++ Sbjct: 104 YNRLVPVAESIRKAGIPLIMIDSAVNGDYALSLIATDNVNAGQKAGKELSSRLPEHAKVA 163 Query: 125 IINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTADAEQL 184 II+F TS Q ERE+G + + E+V Y+ A ++ Sbjct: 164 IISFVQGTSTQI--------------EREQGVRSILEQRGGTEIVGTYYSEGKEERAYEI 209 Query: 185 TRDMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSMLEMLQDGTVQA 243 T+ +L P L GI LNE ++ GAG+ I+ELG G +K+V FDSS +++L++G +Q+ Sbjct: 210 TKKLLLERPDLNGIIGLNEPSTVGAGRAIRELGLVGKVKLVGFDSSVKEVKLLEEGVLQS 269 Query: 244 TVIQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNMLWPENQKLLFPFV 298 T++Q PFN GYL +K AV EG +P + T + +I+ DNM ENQKLLFPFV Sbjct: 270 TIVQKPFNIGYLGIKTAVSVIEGDRMPRHIYTDSVIINKDNMYTEENQKLLFPFV 324 >B1DIU2.1 B1DIU2_9BACL Periplasmic binding protein/LacI transcriptional regulator Length = 327 Score = 183 bits (464), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 113/301 (37%), Positives = 170/301 (56%), Gaps = 20/301 (6%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIIL 60 +I++ + GDYW V LGA+AA KEF + + A E+D D Q+ +++ K D I+L Sbjct: 43 VILRSSEGDYWQNVMLGAQAAVKEFGITMYTTASYDETDADGQMQAARQALATKPDAIVL 102 Query: 61 AASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGY 120 AAS A + A +IPV+++D+ + S K ++Y+G + YEAG ++ L D L Sbjct: 103 AASDDEIFAPFLKEAAERQIPVIAIDSVLTSGKTRSYIGMDNYEAGGQALRELADQLGRQ 162 Query: 121 GEIGIINFTNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVV-QISYTSSNTA 179 G+I II G + R +G L+ A+R+PN+ +V Q++ + Sbjct: 163 GQIAIIAHAA--------------GGINGRLRAQGILDAASRYPNIRIVDQVTCSGEQDI 208 Query: 180 DAEQLTRDMLRNHPR-LRGIATLNETASQGAGKVIQELGPGPL-KMVAFDSSPSMLEMLQ 237 ++ + ++ H R L GI LN AS GA ++ G L K++ FDSSP +LE+LQ Sbjct: 209 CSQTVAEEL---HKRQLDGIIALNTEASIGAAMELRNEQAGNLVKLIGFDSSPELLELLQ 265 Query: 238 DGTVQATVIQNPFNNGYLAVKYAVEATEGIDVPERVDTGTKLIDLDNMLWPENQKLLFPF 297 + +Q ++QNPF+ GYL +KYA+EA G VP RV+ KLID NM W +NQKLLFP Sbjct: 266 ENQLQKLIVQNPFSMGYLGMKYAMEAALGHKVPSRVEIQNKLIDKKNMFWKDNQKLLFPV 325 Query: 298 V 298 V Sbjct: 326 V 326 >C0C8B2.1 C0C8B2_9FIRM Putative uncharacterized protein Length = 324 Score = 170 bits (430), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 98/300 (32%), Positives = 166/300 (55%), Gaps = 19/300 (6%) Query: 1 MIVKMNRGDYWNTVKLGAEAAAKEFNVKLTFKAPDSESDIDEQITMVEESIKQKADVIIL 60 ++ K D+W V +GAEAAA E+N+ LT P++E D + Q M+ E+++ A ++ Sbjct: 38 LVAKSTNTDFWKAVFVGAEAAATEYNMDLTIDGPETEEDYETQNRMIAEAVENGAQALVF 97 Query: 61 AASSYMGLAQVVDRAEYYRIPVVSVDAEVGSAKVKTYVGSNGYEAGQKSAERLIDLLNGY 120 +A + A ++ A + +V +D+ V + V TY+G++ Y AGQ +AE + G Sbjct: 98 SAIDFDANAPAIEEAADKGVKIVVIDSAVNTEDVATYIGTDNYSAGQMAAEAALQGTEGT 157 Query: 121 GEIGIINF-TNATSGQTSSTGTIDFGARDADEREKGFLNYAARFPNVEVVQISYTSSNTA 179 ++GI+N+ N+ +GQ +RE+G ++ A E+V +T + + Sbjct: 158 LKVGIVNYDVNSANGQ---------------DRERGAVDTFAESGRAEIVATIHTLAEAS 202 Query: 180 DAEQLTRDMLRNHPRLRGIATLNETASQGAGKVIQELG-PGPLKMVAFDSSPSMLEMLQD 238 A++ TR ML++HP + + NE S GA + ++EL L MVAFDS+ ++ LQ Sbjct: 203 SAQEDTRKMLQDHPEINALLAFNEPTSVGAAQAVEELHLQNALWMVAFDSNLVTIDALQT 262 Query: 239 GTVQATVIQNPFNNGYLAVKYAVEATEG--IDVPERVDTGTKLIDLDNMLWPENQKLLFP 296 G V A VIQN + GY V+ A + G +V + VDT T++I+ +N+ ++QK+LFP Sbjct: 263 GVVDALVIQNTYEMGYFGVQSAYKLLSGQRSEVEKHVDTATRVINRENLFDLDSQKVLFP 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.131 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,662,984,561 Number of extensions: 66935629 Number of successful extensions: 213106 Number of sequences better than 1.0e-05: 1715 Number of HSP's gapped: 212104 Number of HSP's successfully gapped: 1719 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)